Proliferator-activated Receptor Gamma mRNA Research Articles

Effect of olive leaf extract on the gene expression for lipid metabolism in ovariectomized rats (814.6)

There have not been enough studies regarding estrogen deficiency and lipid metabolism. Even though many postmenopausal women gain excess adipose tissues, the actual mechanism has not been yet fully understood. Thus, we measured mRNA expression after oral administration of olive leaf extract, which has been reported as an antioxidant, hepatoprotective, and hypolipidemic agent, in ovariectomized rats. Ovariectomized rats were administered either 200 or 400 mg/kg body weight of olive leaf extract (n=7 for each group) every day for 10 weeks. Expression of peroxisome proliferator‐activated receptor alpha and acyl‐CoA oxidase mRNA in the liver were significantly higher in the rats administered lower and higher dose of olive leaf extract compared with ovariectomized control. However, mRNA expression of liver carnitine palmitoyltransferase1a was not different among the groups. Expression of peroxisome proliferator activated receptor gamma mRNA in adipose tissue was decreased in the rats with 400 mg/kg body weight of olive leaf extract than ovariectomized control but it was not significantly different among the groups. Adipose triglyceride lipase expression and lipoprotein lipase mRNA did not change compared to ovariectomized control. These results suggest that genes involved in lipid metabolism may be regulated by olive leaf extract administration in ovariectomized rats. Transcription factors for further analyses to expand current results might include sterol regulatory element binding protein 1c and acetyl CoA carboxylase.

PM077 Is The same expression receptor of Peroxisome proliferator-activated receptor gamma (PPARγ) mRNA in human aorta and left ventricle in patients with coronary artery diease ?

PM077 Is The same expression receptor of Peroxisome proliferator-activated receptor gamma (PPARγ) mRNA in human aorta and left ventricle in patients with coronary artery diease ?

Effect of Dietary Conjugated Linoleic Acid (CLA) on Abdominal Fat Deposition in Yellow-feather Broiler Chickens and Its Possible Mechanism

A total of 60 one-day-old Yellow-feather broiler chickens were allotted into treatment and control groups. The treatment group was fed with the diet supplemented with 3% conjugated linoleic acid (CLA) for 48 d, while control group was fed with the diet supplemented with 3% rapeseed oil. Chickens were slaughtered in each group at the age of 49 d, and the blood and the abdominal adipose tissue were sampled. Serum cLeptin and serum cAdiponectin were measured by ELISA. The total RNA was extracted from adipose tissue to measure the abundance of the chicken growth hormone receptor (cGHR), insulin-like growth factor 1 (cIGF-1), insulin-like growth factor I receptor (cIGF-IR), peroxisome proliferator-activated receptor gamma (), cAdiponectin and cAdipoIR mRNA by RT-PCR using -actin as an internal standard. Results showed that the CLA decreased the abdominal fat index by 20.93% (p mRNA in abdominal adipose tissue by 24.74% (p0.05) in the relative abundance of cIGF-1, cIGF-IR, cAdiponectin, and cAdipoIR mRNA in abdominal adipose tissue. The data suggested that CLA inhibited abdominal fat deposition in broiler chicken may be determined by decreasing the GHR available for GH, and by inhibiting the differentiation of preadipocytes via down-regulation of , but independent of IGF and (or) GH-IGF pathway or adiponectin action.

Gene expression of resistin and the effect of conjugated linoleic acid on its expression of obese rat fed with high fat diet

To study the gene expression of the resistin and the effects of conjugated linoleic acid on its expression in white adipose tissue of obese rats fed with high fat diet during the formation of insulin resistance. Male Wistar rats were randomly separated in control group, high-fat group and high fat + conjugated linoleic acid (CLA) group (0.75 g, 1.50 g, 3.00 g per 100 g diet weight), using reverse transcription polymerase chain reaction (RT-PCR) technique to measure the expression level of resistin and peroxisome proliferator-activated receptor-gamma (PPARgamma) mRNA expression. the serum insulin and glucose levels of obese rats were (11.11 +/- 2.73) mIU/L, (5.09 +/- 0.66) mmol/L, and supplement of CLA might decrease hyperinsulinemia and hyperglycemia, in CLA group (0.75 g, 1.50 g, 3.00 g per 100 g diet weight) the serum insulin levels were (6.99 +/- 1.77) mIU/L, (7.36 +/- 1.48) mIU/L, (7.85 +/- 1.60) mIU/L, and glucose levels were (4.28 +/- 0.72) mmol/L, (4.18 +/- 0.55) mmol/L, (4.06 +/- 0.63) mmol/L. The expression of resistin in adipose tissue of obese rat fed with high fat diet was increased as compared with those fed with basic diet. CLA might increase the expression of resistin and PPARgamma in adipose tissue of obese rat. The expression of resistin mRNA of obese rat fed with high fat diet was higher than those fed with basic diet, and CLA might improve the insulin resistance in obese rats and possibly upregulate the expression of resistin through activing PPARgamma.

Effect of conjugated linoleic acid on gene expression of adiponectin of obese rat fed with high fat diet

To study the effect of conjugated linoleic acid (CLA) on expression of adiponectin in white adipose tissue of obese rats. Male Wistar rats were randomly divided into control group, high-fat group and high fat + CLA group (0.75 g, 1.50 g, 3.00 g per hundred gram diet weight), we observed the effect of CLA on serum insulin and glucose levels of obese rats, and the reverse transcription polymerase chain reaction (RT-PCR) technique was used to measure the expression level of adiponectin and peroxisome proliferator-activated receptor-gamma (PPARgamma) mRNA. The serum insulin and glucose levels of obese rats were (11.11 +/- 2.73) microIU/ml, (5.09 +/- 0.66) mmol/L. The supplement of CLA decreased the hyperinsulinemia and hyperglycemia, the serum insulin in CLA group (0.75 g, 1.50 g, 3.00 g per hundred gram diet weight) were (6.99 +/- 1.77) microIU/ml, (7.36 +/- 1.48) microIU/ml, (7.85 +/- 1.60) microIU/ml (P < 0.05), and glucose were (4.28 +/- 0.72) mmol/L, (4.18 +/- 0.55) mmol/L (P < 0.05), (4.06 +/- 0.63) mmol/L (P < 0.05), CLA can increase the expression of adiponectin and PPARgamma in adipose tissue of obese rat. The CLA might improve the insulin resistance of the obese rat and increase the expression of adiponectin mRNA, which might possibly act through activating PPARgamma.