Embryogenic callus which has maintained its embryogenic ability on media without growth regulators for three years has been induced at the base of shoots of a genotype with CMS propagated for a long timein vitro by transferring the shoots onto media richer in inorganic and organic components. The effect of two basal media (MS and PG0) on the intensity and completeness of the proliferation of somatic embryos was examined with different combinations of growth regulators. Pollen fertility was evaluated in 87 plants regenerated from somatic embryos. Cytoplasmic male sterility was conserved in all of them.
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