Project Title Research Articles

Concerns About Fertility Found to Be Major Cause Of COVID-19 Vaccine Hesitancy in the United States

IntroductionAs of July 2021, three vaccines have been issued Emergency Use Authorization (EUA) by the U.S. Food and Drug Administration (FDA) to combat SARS-CoV-2 with over 65% of U.S. adults having received at least one vaccine dose. However, up to 35% of the population are hesitant or refuse to get vaccinated. As reproductive toxicity studies were not conducted prior to EUA, adults have expressed concern about potential adverse effects of the vaccines on fertility and reproductive health.ObjectiveWe evaluated the current reasons for COVID-19 vaccine hesitancy among the unvaccinated U.S. population and identified their demographic characteristics.MethodsAmazon Mechanical Turk (MTurk) was used to survey the vaccine hesitancy reasons amongst unvaccinated U.S. adult population between June 30, 2021-July 1, 2021. The project title listed for survey participants during distribution was “Covid-19 Vaccine Hesitancy Survey”. The study was reviewed by the Institutional Review Board, and it was deemed an exemption. Users with addresses in the U.S., over the age of 18, and received no doses of any coronavirus vaccine at any time were invited to complete an anonymous 32-question survey with an estimated completion time of less than 10 minutes. The first part of the survey focused on identifying attitudes toward the COVID-19 vaccines while the latter queried demographic information such as age, race/ethnicity, and relationship status. Quantitative data was analyzed using by two-sample Z-test on Microsoft Excel (version 16.44) and MATLAB (version R2021a).ResultsA total of 914 unvaccinated adults completed our survey (response rate 91.4%) with 53% of respondents identifying as cis-male and 42% as cis-female. Of the participants, 58% indicated ‘COVID-19 vaccine side effects or other potential unknown long-term effects’ as their reason for remaining unvaccinated and 39% of them believed that ‘COVID-19 vaccines can negatively impact reproductive health and/or fertility’. Among those participants that were concerned that COVID-19 vaccines could impact fertility, 42% (p = 0.010) lived in urban settings, 46% (p > 0.001) were married, and 38% (p = 0.020) of individuals were born outside of the U.S. About 1/2 of the participants stated that more information and research conducted on the COVID-19 vaccines would encourage them to get vaccinated.ConclusionsA large portion of the U.S. population remain fearful of the potential side effects associated with the coronavirus vaccines and more specifically negative impacts to their future reproductive health. These results objectively evidence that fertility concerns are significantly contributing to vaccine hesitancy and may continue to be a barrier for years to come if no interventions are made. With almost half of the participants yearning for more information and research this highlights the need for intense investigation and publicly available data on the effect of coronavirus vaccines on fertility.DisclosureNo

Advising, an Indispensable Element in Training Students. Usefulness of POSDRU Grants

The present work aims at improving the ability of innovation of institutions for education and training through advisory activities for students in order to prepare them for society and for the environment. In this paper are presented efficiency analysis of career counseling activities in the framework of a European project. Demonstration of the appropriateness of professional counselors will be made on the basis of a pilot group of students advising in career as part of a European project, co-financed by the EUROPEAN SOCIAL FUND, Sectoral operational program Human Resources Development 2007-2013, Priority axis no.2 ""Linking learning with lifelong labor market"", Major field of intervention no. 1.3 “Transition from school to life”, Project title: ""Partnership developed for advice and practice of students in order to increase their employability- CONPRACTIS"", Contract no.: POSDRU/189/2.1/G/156607. Counselling and career guidance are important for students in order to gain the relevant skills and competencies for a job and the right attitude towards work, sense of responsibility, gaining practical experience in the workplace, the ability to solve new problems and to understand the context in which action must be taken; the ability to adapt to any working conditions, the provision of ""affective competence"" relevant, appropriate social skills. Many studies and European statistics highlight the relationship of dependency between the welfare economy and education level but, at the same time, draw attention to the danger of ""scatter"" one of the most valuable resources-human resources. Correspondence between the quality of vocational training, on the one hand, and the requirements of the labor market on the other hand, is essential in a knowledge-based economy. Research shows that the unemployment rate among young people may cause permanent sequelae, such as an increase in the risk of being unemployed in the future, low levels of future earnings, loss of human capital, the transmission of poverty from one generation to another or a lesser motivation. One of the reasons identified is the lack of experience to submit to the interview, on condition that the student does not have the experience of another job, but neither-and leverages and presents it in a way convincing powers and abilities held when â when attending the interview.

Cues that Work: Designing the Optimal Restaurant Crowdfunding Campaign in the US

ABSTRACT This study explores how restaurant entrepreneurs incorporate various intrinsic and extrinsic cues into the design of reward-based crowdfunding campaigns to maximize their probability of success. This study aims to identify the optimal levels of each of these cues to guide entrepreneurs’ strategic behavior. Based on the cue utilization theory and the concept of optimal arousal, this study collected restaurant project data from Kickstarter. Linguistic Inquiry and Word Count (LIWC) was also used to quantify linguistic cues in the project title. Linear and quadratic terms of cues were included in the logistic regression model. An optimal campaign should have 13 pictures, 2 videos, a detailed but succinct description, and many rewards. The project title should have a neutral and conservative style instead of being extremely positive.

Using single cell type proteomics to identify Al-induced proteomes in outer layer cells and interior tissues in the apical meristem/cell division regions of tomato root-tips

Aluminum (Al) toxicity primarily targets the root tips, inhibiting root growth and function and leading to crop yield losses on acidic soils. Previously we reported using laser capture microdissection (LCM) proteomics to identify Al-induced proteins in the outer layer cells in the transitional zone of tomato root-tips. This study aims to further characterize Al-induced proteomic dynamics from the outer to interior tissues, thus providing a panoramic view reflecting Al resistance in the root tip as a whole in tomatoes. Three types of cells were isolated via LCM from the basal 350–400 μm (below cell elongation regions) of root tips using tomato (Solanum lycopersicum) ‘Micro-Tom’ plants. Type I and Type II were from Al-treated plants. Type I included cells of the outer three layers, i.e., the epidermis and cortex initials and the quiescent center (QC) in root apical meristem (RAM), and Type II possessed the interior tissues of the same region. Type III contained cells from the non-Al-treated root tips collected in the same region as Type I. Two tandem mass tag (TMT) proteomics analyses with three biological replicates for each sample type were conducted. The TMTexp1 (comparing Type I and Type II) identified 6575 quantifiable proteins and 178 different abundance proteins (DAPs). The TMTexp2 (comparing Type I and Type III) identified 7197 quantifiable proteins and 162 DAPs. Among all quantified proteins (7685) from the two TMT experiments, 6088 (79%) proteins, including 313 DAPs (92% of the 340 total), were identified in all tissues. A model reflecting the tissue-specific Al-resistance mechanism was proposed, in which the level of the citrate transporter MATE protein, involved in Al exclusion, accumulated to the highest level in the outer-layer cells but decreased toward the interior of root-tips (which concurs with the tissue-specific importance in Al resistance). Proteins for biosynthesis of ethylene and jasmonic acid, proteolytic enzymes, stress-responsive proteins, and cell wall modeling were affected by Al treatment, some in a cell type-specific manner. The KEGG metabolite pathways enriched with these DAPs changed depending on the cell types. This study demonstrated the advantage of using the tissue/cell-specific analysis for identifying proteins and their dynamic changes directly associated with Al resistance in the root-tip region. The proteomics datasets have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository (https://www.ebi.ac.uk/pride/) with the dataset identifier as PXD021994 under project title: Proteomics studies of outer and inner cellular layers of tomato root-tips for Al stress, Project DOI: 10.6019/ PXD021994; and PXD018234 under Project title: Al-induced root proteomics changes in stress-acclimated tomato plant, Project DOI: https://doi.org/10.6019/PXD018234. SignificanceThis paper presents the method of using laser capture microdissection (LCM) to collect homogenous cell-type specific tissue samples from the outer layers and inner central regions of tomato root-tips. The tandem mass tag-proteomics analysis showed that the outer-layer cells expressed proteomes that were different from the inner tissues of Al-treated root-tips; proteins related to resistance/tolerance to Al toxicity were highly accumulated in the outer-layer cells. Furthermore, the Al-treated outer-layer cells expressed proteomes which were different from the non-Al treated counterpart cells. This study has provided the first dataset of proteins differentiating from the outer to inner layers of cells in Al-treated root-tips. It provided convincing experimental evidences demonstrating the single-cell type proteomics as a powerful analytical approach to identify Al tolerance mechanisms in plants. The analytical procedure of LCM-tandem mass tag-quantitative proteomics analysis has a broad application for proteomics analysis of spatially separated cells in complex tissues.

Structural variations on the surface of metallic products at laser marking

Laser marking of products and details is used more and more wide in different production areas, because it has undisputable advantages in comparison with other marking methods. Action of laser beam on the surface of marking product is the principal feature of laser marking. It is accompanied with local heating of this surface and its partial melting together with material evaporation from the surface contact point. Influence of laser impulse action on efficiency of marking on the surface of machine-building details is examined in this work. The samples of 08Kh18N10 steel were used as material. Marking was conducted by the system “MiniMarker 2-20A4”, QR code was used as a marker. The marking parameters were optimized via the method of experimental design. The optimal marking procedure for getting maximal contrast of QR code was calculated and experimentally confirmed. Steel surface roughness was analyzed using profilograms. Contrasting effect and roughness of code were determined and relationship between these parameters was revealed. Contrast effect of marking increases with elevation of roughness. However, roughness has less input in increase of contrast effect comparing with colour hue of the surface of an information block and substrate. The link between surface roughness and contrast effect of marking is shown. Possibility of contrast effect assessment of QR code using profilometer is suggested. The reported study was financially supported by the Ministry of Science and Higher Education of the Russian Federation, research agreement No. 075-11-2019-066 of 22.11.2019, project title “Development of high-tech production of equipment and technologies for laser coding of transported goods and their optical identification for the implementation in modern material flow management systems” (within the framework of decree of the Government of the Russian Federation, No. 218 of 09.04.2010).

Vicki Funk's legacy continues – The IAPT Small Collections Grant

Vicki Funk's legacy continues – The IAPT Small Collections Grant

Correction to: Perceptions of Support in Chinese Mothers of a Child with Intellectual Disability

In the original published version of this article the Funding Information was not captured. Thus, the authors wish to add this information ‘National Office for Education Sciences Planning in China (Project Title: Parenting and support for families of children with disabilities from an ecological perspective, Project Number: CHA170264)’. This erratum is presented to fix the error. The original article has been corrected.

Effect of Textual Features on the Success of Medical Crowdfunding: Model Development and Econometric Analysis from the Tencent Charity Platform.

BackgroundMedical crowdfunding utilizes the internet to raise medical funds. Medical crowdfunding has developed rapidly worldwide; however, most medical crowdfunding projects fail to raise the targeted funds. Therefore, a very important research problem that has not received sufficient attention from the existing literature is identifying which factors affect the success of medical crowdfunding projects.ObjectiveThe aim of this study was to examine the effect of textual features of medical crowdfunding projects on their success rate using 4903 real projects from the Tencent Charity platform, a well-known medical crowdfunding platform in China. In particular, according to Aristotle’s theory of persuasion, we divided the project text of medical crowdfunding into the project title and project details, which were analyzed from two perspectives (existence and extent) to explore their respective impacts.MethodsWe established a research framework to meet our research goals. The process was divided into five main parts. We first collected data from Tencent Charity using Python programs and cleaned the datasets. Second, we selected variables and built the research model based on previous studies and the theory of persuasion. Next, the selected variables were extracted from the project text. We then performed econometric analysis using multiple regression analysis. Finally, we evaluated the results of econometric analysis to extract knowledge.ResultsIn the project title, the presence of the patient’s disease (P=.04) and occupation (P=.01) had a positive impact on the success rate of fundraising, whereas the presence of age (P<.001), gender (P=.001), and negative emotions (P=.04) had a negative impact. In the project details, the presence of the patient’s occupation (P=.01), monetary evidence (P=.02), and negative emotions (P=.04) played a positive role in the fundraising success rate, whereas the presence of age (P<.001) and positive emotions (P<.001) played a negative role. Moreover, in the project details, high-frequency monetary evidence (P=.02) and negative words (P=.02), as well as a short narrative length (P=.01) were conducive to succeeding in medical crowdfunding. Younger patients were more likely to obtain a higher success rate in medical crowdfunding. For patients whose occupations were national civil servant, professional skill worker, clerk, business and service worker, solider, child, student, and public-spirited person, the success rate of fundraising decreased sequentially.ConclusionsThis study collected 4903 valid data from Tencent Charity, and identified which factors in the project text play an important role in the success rate of medical crowdfunding from the perspective of existence and extent. We found that in addition to the project details, the features of the project title also have an important impact on the success rate of fundraising. These findings provide important theoretical and managerial implications for medical crowdfunding.

Uji Kemiripan Kalimat Judul Tugas Akhir dengan Metode Cosine Similarity dan Pembobotan TF-IDF

Deli Husada Health Institute is a health campus that has been established for 34 years, currently it has 30000 students, each student at the final level will submit a final project of study program every year, each student before doing his final project report must provide the title of an assignment report. Finally, to the study program, to reduce the level of similarity in the title of the student's final report, the study program usually conducts a manual check, the result that appears is that it is not effective in determining the title of the final project for students, so that it creates quite a lot of similarities between students. So that many final project reports look the same. With the above conditions, the Sentence Similarity Test of the Final Project Title was carried out with the Cosine Similarity Method and TF-IDF Weighting at the Deli Husada Delitua Health Institute Campus. At the end of the test results on the training data against the training data, the results obtained were 43% of the titles in Submitted is not eligible to be submitted again and 53% is eligible to be submitted as the title of the final project because it has high similarities to the title of the final project report. And get the average time 0.12117 in minutes

The effect of vitamin C on sociability in a juvenile zebrafish pesticide-induced model of autism spectrum disorder

IntroductionAutism spectrum disorder (ASD) is a multi-factorial disease characterized by impairments in social interaction, communication and repetitive behaviors. The necessity of developing an adequate treatment for ASD is essential. There is an increase in clinical studies assessing the positive effects of vitamins in ASD children. Vitamin C (vit. C) is implicated in biosynthesis of neurotransmitters and in protein metabolism.ObjectivesThis study evaluated the possible effect of vit. C on zebrafish sociability after a single insecticide mixture administration as inductor for ASD.MethodsA single dose of insecticide mixture (600 μg L-1 fipronil and 600 μg L-1 pyriproxyfen) was administrated to zebrafish juvenile. Vit. C (25 μg L−1) was daily administrated during 14 days. A control group simulated the administration of insecticide mixture and vitamin. Each animal was tested in the experimental tank designed for the social interaction test. The trials were recorded and analysed using EthoVision XT 11 (NOLDUS, Netherlands). The locomotor activity parameters and the time spent next to the group were measured. Each trial had 4 minutes duration.Results We have found no significant differences in the average levels between pre-treatment and treatment days (P&lt; 0.05 ANOVA) regarding the locomotor activity parameters. Significant changes in sociability were observed for the group exposed to insecticide mixture and for vit. C group (P &gt; 0.05 ANOVA). It was also found that 14 days vitamin administration can lead to sociability improvements after a single administration of mixture insecticide.ConclusionsThe results of the current study bring some positive insights for the future of ASD therapy.Conflict of interestThis work was co-funded by the European Social Fund, through Operational Programme Human Capital 2014-2020, project number POCU/380/6/13/123623, project title

Perancangan Arsitektur Sistem Informasi Tugas Akhir dan Skripsi Berbasis Website di Universitas Ma’soem

One of the activities at Ma'soem University is the Registration of Final Projects for Diploma Three (D3) students and Thesis for Undergraduate students (S1). Currently, the registration process for the Final Project and Thesis is still done manually, where students must come directly to the campus to register, pay and fill out the title submission form. Meanwhile, for the preparation of seminar schedules, honoraria and final and thesis report, data processing is still using Ms. Excel. From these activities, several obstacles were found regarding information on Final Projects and Thesis, including: 1. Limited payment and registration services for Final Projects and Thesis, which can only be served on campus during working hours and days from Monday to Saturday 07.30 to 21.30 WIB. 2. Filling in the Final Project and Thesis forms manually, there is a risk of damage and loss of data. 3. Do not have a database yet, so that it is difficult to validate data on the same Final Project Title and Thesis submitted by students. 4. The process of making seminar and final project honorarium reports and thesis sessions takes a long time, and cannot be generated automatically. In this study, the system development method used is the OOAD (Object Oriented Analysis and Design) method with the RUP (Rational Unified Process) model, which consists of several stages, namely: Business Modeling, Requirments, Analysis and Design, Implementation, Test, Deployment. As for the system development tools using UML (Unified Modeling Language) which consists of: Use Case Diagrams, Activity Diagrams and Class Diagrams. With the design of the information system architecture of this Final Project and Thesis, it produces a blueprint that can: Provide payment and registration services for Final Projects and Theses that are not limited by space and time, minimize the occurrence of damage and data loss, make it easier to validate submissions for the title of Final Project the same and can facilitate the process of making seminar and final project honorarium reports and thesis reports so that they can be generated automatically.

Perancangan Arsitektur Sistem Informasi Tugas Akhir dan Skripsi Berbasis Website di Universitas Ma’soem

One of the activities at Ma'soem University is the Registration of Final Projects for Diploma Three (D3) students and Thesis for Undergraduate students (S1). Currently, the registration process for the Final Project and Thesis is still done manually, where students must come directly to the campus to register, pay and fill out the title submission form. Meanwhile, for the preparation of seminar schedules, honoraria and final and thesis report, data processing is still using Ms. Excel. From these activities, several obstacles were found regarding information on Final Projects and Thesis, including: 1. Limited payment and registration services for Final Projects and Thesis, which can only be served on campus during working hours and days from Monday to Saturday 07.30 to 21.30 WIB. 2. Filling in the Final Project and Thesis forms manually, there is a risk of damage and loss of data. 3. Do not have a database yet, so that it is difficult to validate data on the same Final Project Title and Thesis submitted by students. 4. The process of making seminar and final project honorarium reports and thesis sessions takes a long time, and cannot be generated automatically. In this study, the system development method used is the OOAD (Object Oriented Analysis and Design) method with the RUP (Rational Unified Process) model, which consists of several stages, namely: Business Modeling, Requirments, Analysis and Design, Implementation, Test, Deployment. As for the system development tools using UML (Unified Modeling Language) which consists of: Use Case Diagrams, Activity Diagrams and Class Diagrams. With the design of the information system architecture of this Final Project and Thesis, it produces a blueprint that can: Provide payment and registration services for Final Projects and Theses that are not limited by space and time, minimize the occurrence of damage and data loss, make it easier to validate submissions for the title of Final Project the same and can facilitate the process of making seminar and final project honorarium reports and thesis reports so that they can be generated automatically.

Harnessing Technology for Research During the COVID-19 Pandemic: A Mixed Methods Diary Study Protocol

Project Title:Psychosocial Reactions to a Global Pandemic: A Diary Study During COVID-19.Design:The study uses a mixed methods design that includes both a cross-sectional quantitative survey and qualitative diary entries submitted online eight times by student participants.Context of Study:The study timeline spans the full shutdown, phase 1 reopening, and phase 2 reopening of New Orleans Louisiana.Objectives:The study has three primary objectives: (1) To better understand the experiences of Social Work Students during the COVID-19 pandemic; (2) To explore risk and protective factors that influences Social Work Students’ ability to cope with stressors and changes associated with the COVID-19 pandemic; and (3) to track changes over time in coping among Social Work Students during the COVID-19 pandemic.Study Population:Current Master of Social Work (MSW) Students enrolled at Tulane University during the COVID-19 Pandemic will be invited to participate in this study.

The 15th Medical Quality and Safety Society Academic Meeting Project title &quot;Symposium: Safety Management of Medical Radiation&quot; -Current status and issues after the enforcement of the revised law- &quot; Words of the planner and chair

The 15th Medical Quality and Safety Society Academic Meeting Project title &quot;Symposium: Safety Management of Medical Radiation&quot; -Current status and issues after the enforcement of the revised law- &quot; Words of the planner and chair

Predictors of death and heart transplantation in biopsy-proven myocarditis: a machine-learning approach

Abstract Background Risk stratification for death and heart transplantation (HTx) in myocarditis is complex. A random forest (RF) is a tree-based machine learning technique (MLT) which is being increasingly used for clinical data analysis; it allows the detection of complex relationships between the outcome of interest and the covariates, overcoming the limits of traditional statistical analysis (i.e. regression approaches). Purpose To assess the potential role of clinical and diagnostic features at presentation as predictors of death and HTx in biopsy (Bx)-proven myocarditis using RF. Methods From January 1993 to August 2019, we consecutively enrolled 357 patients with Bx-proven myocarditis (65% male, median age 39 years, interquartile range (IQR) 26–51). An RF approach for survival data was used. Variables included in the analysis were: histology type by Bx, NYHA, type of presentation (infarct-like, arrhythmia, heart failure), viral genome detection on Bx, serum antiheart (AHA), antiintercalated disk (AIDA), anticardiac endothelial cells (AECA), antinuclear (ANA) autoantibodies, immunosuppressive therapy, cardiac catheterisation (left ventricular enddiastolic volume (LVEDV), mean capillary wedge pressure, right and left ventricular enddiastolic pressure) and 2-D echocardiographic measures (LVEDV, left ventricular ejection fraction (LVEF) at presentation and at follow-up, right ventricular fractional area change (FAC%), right ventricular diastolic area). Results The median follow-up time was of 1352 days (IQR 423.25–2535.75). At the end of follow-up, 42 patients were dead or transplanted. The 1-year, 5-year, and 10-year survival probabilities were of 0.928, 0.854, and 0.817, respectively. The most relevant predictors of death or HTx identified by the RF algorithm (according to the variable importance measure) were histological type, NYHA, clinical presentation, LVEF, and FAC%. Among the circulating auto-antibodies AECA were found to be the most important. Histological type was the strongest predictor of death/HT (100% relative importance, (RI)), giant cell myocarditis having a lower survival probability compared to other types. The next stronger predictors were advanced (III-IV) NYHA and heart failure presentation with lower survival probabilities (90% and 84% RI respectively). AECA-positive patients had lower survival probability compared to AECA negative ones (20% RI). The RF algorithm revealed an excellent predictive performance in the correct identification of all alive patients, with only 5 dead patients being misclassified (balanced accuracy 94%). Conclusions Autoimmune features, i.e Giant cell myocarditis and AECA, as well as severity of heart failure and of left ventricular disfunction at presentation were the strongest predictors of dismal prognosis. Our RF approach provides a new automated powerful tool for accurate risk stratification for death/HTx in Bx-proven myocarditis. Funding Acknowledgement Type of funding source: Public Institution(s). Main funding source(s): Budget Integrato per la Ricerca dei Dipartimenti (BIRD, year 2019), Padova University, Padova, Italy (project Title: Myocarditis: genetic background, predictors of dismal prognosis and of response to immunosuppressive therapy.)

International R&D Collaboration for a Global Aging Society: Focusing on Aging-Related National-Funded Projects.

An international research and development (R&D) collaboration for aging-related projects is necessary to alleviate the severe economic/healthcare/humanitarian challenges of a global aging society. This study presents a practical/systematic framework that enables the provision of information on the research goals, the status of science and technology, and action plans of aging-related program development processes. We used data on aging-related national-funded projects from the United States of America, the European Union, the United Kingdom, Japan, and Korea. We collected data on 6318 national-funded projects, subsequently designating research fields to each project. By analyzing the content of the projects, their representative research fields, and the associated keywords, we assessed the general goals of six different research fields. To recognize the current scientific capabilities of these research fields, we divided the projects by clusters. We provided information on research organizations, specific goals (i.e., project title), project periods, and the funding related to the projects. These may be used by stakeholders in various governments/institutions/industries during future discussions regarding the establishment of an international R&D collaboration strategy. The approach we proposed may facilitate the linkage between knowledge and action during strategy development by maximizing scientific legitimacy, developing consensual knowledge, and minimizing diverging opinions among stakeholders.

0107 Neurocognitive Functioning in Individuals with Comorbid Insomnia and Sleep Apnea: Baseline Functioning and Impact of Treatment

Abstract Introduction Neurocognitive impairments are common in individuals with insomnia and sleep apnea separately, however, the evidence for impairment in comorbid insomnia and sleep apnea (COMISA) is much less clear. We aim to determine a neurocognitive profile for individuals with COMISA, and evaluate the benefits of treatment on neurocognitive performance. Methods Participants with COMISA (n=45; 51.1% female; mean age=52.07±13.29) were from a two-phase, 3-arm randomized clinical trial combining Cognitive Behavioral Therapy for insomnia (CBT-I) with Continuous Positive Airway Pressure (CPAP) administered sequentially or concurrently compared to CPAP alone. The unique groups from this factorial design were used to establish baseline impairment and the effects of treatment on neurocognitive functioning. Results Individuals with COMISA had a slower reaction time (365.66±157.39) and more lapses (9.70±16.16) on the Psychomotor Vigilance Task than has been reported in the literature for insomnia and OSA separately. Our COMISA participants had mean scores indicating mild impairment for their age, based on normative data, on digit span (9.71±2.47 forward; 3.95±2.63 backward), verbal paired associates (27.71±11.54 immediate; 8.69±3.16 delayed; 37.17±2.97 recognition), Digit Symbol Substitution (68.83±12.33) and the Stroop (35.46±7.26 color/word; -3.19±7.5 interference). The only significant treatment outcome was in the acute effect after CBT-I on digit span forward (1.7 fewer digits remembered after CBT-I vs. 0.2 fewer after no treatment, t(32)=-2.1, p=0.047). This effect was no longer significant after controlling for multiple comparisons. Conclusion This is the first study to report baseline neurocognitive impairments and effects after treatment for individuals with COMISA. Baseline impairments appear to be more significant than reported in the literature for conditions individually and published norms. Results indicate a worsening in neurocognitive functioning after CBT-I, however the numbers within the groups are small, and this effect disappears after controlling for multiple comparisons. Future studies are necessary to provide a conclusive answer regarding the effects of treatment on neurocognition. Support NIH/NHLBI (R01 HL114529-03S1) Ong 1/7/2015 - 6/30/2017 Project Title: Multidisciplinary Treatment for Obstructive Sleep Apnea and Insomnia - Research Supplement to Promote Diversity in Health-Related Research

IOAT in the ED – Lessons Learned

Emergency Strategic Clinical NetworkTM &#x0D; Quality and Innovation Forum Presentation Proposal&#x0D; Name: xx&#x0D; Position (e.g. patient care manager, professor): Manager&#x0D; Primary Affiliation: (AHS) Other: AHS&#x0D; Project Title: iOAT in the ED – Lessons Learned&#x0D; Hospital: All adult sites in Calgary &#x0D; Location: Calgary&#x0D; Team Members: xx &amp; xx&#x0D; Background&#x0D; Deaths related to opioid poisoning have continued to climb over the last few years. The Injectable Opioid Agonist Treatment program (iOAT) provides injectable hydromorphone to those individuals with moderate to severe opioid use disorder and a history of injection drug use who have been unsuccessful with oral OAT and continue to be at high risk for opioid poisoning. Working with the emergency departments (ED) was identified as a critical step in the initial roll out of iOAT.&#x0D; Implementation &#x0D; The iOAT program began operating in October 2018. The clinic provides prescribed hydromorphone to clients within the program. Additionally, the team is comprised of physicians, nurse practitioners, nurses, social worker, peer support workers and administrative support to provide comprehensive wrap around care to every client that is registered to the program. It was recognized early on that the clients that were being served by iOAT were also high users of the ED and UCCs. Being part of iOAT became a factor that needed to be considered when these clients presented to the ED due to their prescription of hydromorphone. Working with management, medical leadership, and nurse educators, support and education were provided to ensure that iOAT clients were provided with optimal care when in the ED. Ongoing communication has been the primary strategy that has been used.&#x0D; Evaluation Methods &#x0D; The evaluation for this project has been informal and ongoing. The medical team at iOAT has worked with the medical team for the Calgary EDs to develop a detailed treatment plan that is visible on SCM. Telephone and emails have been the primary mode of feedback for both parties, and the plan is adjusted as necessary along the way.&#x0D; Results&#x0D; Improving the knowledge and understanding for all staff involved to understand iOAT and the role of the ED has been demonstrated to be effective when clients stay in the ED and don’t leave against medical advice, which likely occurred before. Additionally, the trust that is built within the iOAT clinic is maintained when the ED is a partner in care and as appropriate, provides them with the dosing that they would normally receive at iOAT.&#x0D; Advice and Lessons Learned &#x0D; 1) Involve the emergency department management in planning or initial implementation&#x0D; 2) Communicate, Communicate, Communicate&#x0D; 3) Use continuous feedback to adjust to find the best strategies to provide patient care

A 12-month follow-up of a transdiagnostic indicated prevention of internalizing symptoms in school-aged children: the results from the EMOTION study

BackgroundAnxious and depressive symptoms in youth are highly prevalent, are often comorbid and have a high rate of relapse. Preventive interventions are promising, but follow-up results are lacking. The transdiagnostic EMOTION program is an indicated preventive cognitive behavioral therapy (CBT) intervention targeting children aged 8–12 years.MethodsThe present study investigates the 12 months follow-up effects of the EMOTION intervention in a cluster randomized controlled trial (RCT) with 795 children that included both child self-reports and parental reports.ResultsMixed model analyses showed a larger decrease of symptoms in the intervention group than in the control group for child self-reported anxious symptoms (The Multidimensional Anxiety Scale for Children (MASC) difference 4.56, CI 1.83 to 7.29, p = .001). Parental reports for both anxious (MASC difference 2.50, CI .26 to 4.74, p = .029) and depressive (The Mood and Feelings Questionnaire-short form (SMFQ) difference 1.55, CI .83 to 2.26, p ≤ .001) symptoms in children also showed a reduction. No statistically significant difference was found for child self-reported depressive symptoms (SMFQ difference .69, CI − .22 to 1.60, p = .139).ConclusionThe transdiagnostic EMOTION program has shown the potential for long-term reductions in symptoms of both anxiety and depression in school-aged children. However, results regarding depressive symptoms must be considered preliminary as only parental report indicated effect.Trial registration The regional ethics committee (REC) of Norway approved the study. Registration number: 2013/1909; Project title: Coping Kids: a randomized controlled study of a new indicated preventive intervention for children with symptoms of anxiety and depression. ClinicalTrials.gov Identifier; NCT02340637.

Al-induced proteomics changes in tomato plants over-expressing a glyoxalase I gene

Glyoxalase I (Gly I) is the first enzyme in the glutathionine-dependent glyoxalase pathway for detoxification of methylglyoxal (MG) under stress conditions. Transgenic tomato ‘Money Maker’ plants overexpressing tomato SlGlyI gene (tomato unigene accession SGN-U582631/Solyc09g082120.3.1) were generated and homozygous lines were obtained after four generations of self-pollination. In this study, SlGlyI-overepxressing line (GlyI), wild type (WT, negative control) and plants transformed with empty vector (ECtr, positive control), were subjected to Al-treatment by growing in Magnavaca’s nutrient solution (pH 4.5) supplemented with 20 µM Al3+ ion activity. After 30 days of treatments, the fresh and dry weight of shoots and roots of plants from Al-treated conditions decreased significantly compared to the non-treated conditions for all the three lines. When compared across the three lines, root fresh and dry weight of GlyI was significant higher than WT and ECtr, whereas there was no difference in shoot tissues. The basal 5 mm root-tips of GlyI plants expressed a significantly higher level of glyoxalase activity under both non-Al-treated and Al-treated conditions compared to the two control lines. Under Al-treated condition, there was a significant increase in MG content in ECtr and WT lines, but not in GlyI line. Quantitative proteomics analysis using tandem mass tags mass spectrometry identified 4080 quantifiable proteins and 201 Al-induced differentially expressed proteins (DEPs) in root-tip tissues from GlyI, and 4273 proteins and 230 DEPs from ECtr. The Al-down-regulated DEPs were classified into molecular pathways of gene transcription, RNA splicing and protein biosynthesis in both GlyI and ECtr lines. The Al-induced DEPs in GlyI associated with tolerance to Al3+ and MG toxicity are involved in callose degradation, cell wall components (xylan acetylation and pectin degradation), oxidative stress (antioxidants) and turnover of Al-damaged epidermal cells, repair of damaged DNA, epigenetics, gene transcription, and protein translation. A protein–protein association network was constructed to aid the selection of proteins in the same pathway but differentially regulated in GlyI or ECtr lines. Proteomics data are available via ProteomeXchange with identifiers PXD009456 under project title ‘25Dec2017_Suping_XSexp2_ITAG3.2’ for SlGlyI-overexpressing tomato plants and PXD009848 under project title ‘25Dec2017_Suping_XSexp3_ITAG3.2’ for positive control ECtr line transformed with empty vector.