Estimated glomerular filtration rate (eGFR) is a measure of renal filtration and clearance of serum creatinine and is conventionally used to characterize the progressive decline in renal function (RF). Assessment of RF and renal health (RH) is traditionally believed to be age-dependent. However, in the absence of cardiometabolic diseases (hypertension, diabetes, hyperlipemia, etc.), this may not be the case. Recently, novel markers of RH and RF support the notion that age is a secondary factor influencing renal decline. PURPOSE: To determine the magnitude of age as an influencing factor involved in the decline of RF with novel markers of RH and function in the absence of cardiometabolic risk factors. METHODS: Fifty-four participants (n = 27 men; n = 27 women; age 33.4 + 12.5 yr; height 171.7 + 10.9 cm; weight 77.9 + 15.9 kg; BMI 26.5 + 5.5; SBP 120 + 10.4; DBP 77.7 + 6.7; CHOL 174 + 30; and GLU 95 + 7) completed a single health assessment to quantify renal health and function. Blood and urine samples were collected by the same technician under standardized conditions and stored at -80 °C until project completion. Serum creatinine (sCR), urine creatinine (uCr), cystatin C (CyC), and urine epidermal growth factor (uEGF) were used to calculate estimates of renal health and function via uEGF/uCr ratio (uEGFR), eGFR - modification of diet in renal disease (MDRD), CKD-EPI, and SCr/CyC eGFR. Renal health and function markers were analyzed and compared in age groups (20s, 30s, 40s, 50s) using 4 (group) by 1 (sample) ANOVAs. RESULTS: There were no significant differences between age groups in the biomarkers and estimates of RH and RF. sCR (F = 0.17, p = 0.91), uEGF (F = 0.87, p = 0.46), CyC (F = 1.98, p = 0.13), CyC eGFR (F = 2.81, p = 0.10), MDRD (F = 2.08, p = 0.12), CKD-EPI (F = 0.34, p = 0.80), and SCr/CyC eGFR (F = 2.05, p = 0.12). uEGFR was significantly different between (F = 1.93, p = 0.14) different between age groups. uCr was significantly different ((F = 5.33, p = 0.003) between 40s and 50s. CONCLUSION: Changes in RH and RF appear to be independent of age in the absence of cardiometabolic diseases, indicates RH and RF could potentially be maintained in adulthood, middle age, and possibly attenuated in the senior years with the continued absence of cardiometabolic diseases.