Sampling Program Research Articles

Size and resin fractionations of dissolved organic matter and characteristics of disinfection by-product precursors in a pilot-scale constructed wetland

Abstract Controlling the formation of disinfection by-products (DBPs) is a major issue in the drinking water industry, and understanding the characteristics of DBP precursors in treatment processes for micro-polluted raw water is key to improving water quality. In this study, a sampling program was undertaken to investigate the fate of dissolved organic matter (DOM) and the characteristics of DBP precursors in a pilot constructed wetland imitating the Yanlong Lake ecological project. Using XAD resin adsorption and ultrafiltration techniques, the dissolved organic carbon, UV254, and DBP formation potential (DBPFP) were measured in different DOM fractions in raw water and wetland effluents. After the constructed wetland treatment, the low molecular weight fraction (<3 kDa) of DOM and DBPFP generally showed a decreasing trend along the water path, while the high molecular weight fraction (>3 kDa) of DOM increased. The specific DBPFP (SDBPFP) was much higher in the <1 kDa fraction than in the other fractions. Although the hydrophobic fraction of DOM was the most abundant in all stages of the wetland treatment, the SDBPFP of the hydrophilic fraction was higher than that of the hydrophobic fraction. Furthermore, compared with raw water, the DOC, UV254 and DBPFP in the treated wetland effluents increased; however, all of the chemical DOM fractions exhibited decreased SDBPFP in accordance with a decrease in the specific ultraviolet absorbance during wetland treatment. These conclusions indicate that the DOM produced by the wetland system may generate DBPs less readily compared with the DOM of raw water.

Monitoring of Bioaerosol Emission from a Sludge Composting Facility

This paper presents results of one-year monitoring of bioaerosol dispersion from a full-scale sludge composting facility in the east coast of the USA. By using two-stage Andersen air samplers with a sequential sampling procedure developed in this study, a total of 24 sets of bioaerosol samples were collected on petri dishes for plate counting. The sampling program utilized a computer air dispersion model (ISCST) to predict the downwind distance at the maximum concentration. Field samplings were performed at upwind, onsite, and predicted downwind locations. The results of this study conclude that the 95% confidence intervals esti- mated for the background concentrations were: 75-173 cfu/m

Disentangling the links between habitat complexity and biodiversity in a kelp-dominated subantarctic community.

Habitat complexity is one of the most important factors modulating species diversity. This feature comprises several interrelated attributes, such as number, size, and spatial arrangement of complexity‐forming elements. However, the separate and joint effects of these attributes on diversity and community structure are still not well understood. Here, we assess the relationships between several structural‐complexity attributes of the subantarctic kelp Lessonia flavicans and species richness, total abundance, and structure of kelp‐associated macrobenthic communities. We predicted that longer thalli and larger holdfasts favor greater species richness and total abundance of invertebrate organisms. To test the prediction, an observational sampling program was established in two sites of the Strait of Magellan. Uni‐ and multivariate analyses revealed both positive and negative effects of kelp structural‐complexity attributes on diversity. Holdfast diameter and maximum frond length, followed by thallus wet weight, had the strongest positive fits to species richness and total abundance; the number of stipes, on the other hand, was negatively associated with both response variables. Longer fronds were associated with greater abundances of spirorbid polychaetes. Larger holdfasts supported larger abundances of Nereididae and Terebelidae polychaetes and the limpet Nacella mytilina. Contrarily, kelps with longer fronds and more stipes supported fewer amphipods. In this way, we demonstrate that different dimensions of habitat complexity can have contrasting effects on diversity and community structure, highlighting the fundamental role of multiple dimensions of kelp habitat complexity for local biodiversity.

Possible human health risk of some heavy metals from consumption of tilapia fish from Lake Mariut, Egypt.

Lake Mariut Main Basin (MB) is not only one of the three basins composing Lake Mariut (LM) but is also the main source of the popular tilapia fish to Alexandrian people. Unfortunately, this basin was consistently for about 50 successive years receiving a continuous discharge of agricultural, sewage, and industrial effluents. This has led to contamination of the flesh of its tilapia fish living there particularly with some toxic heavy metals like Cd and Pb. Lately, in 2010, a rehabilitation program was adopted and carried out to save this vital MB from such intensive pollution. This had been achieved by diverting all those polluting sources. The present work is made after elapsing about 7years from the diversion process date, to assess and evaluate the levels of those two metals (in addition to the other four one's Fe, Cu, Cr, and Zn) in the edible flesh part besides the liver and gills of this fish (Nile tilapia spec., Oreochromis niloticus), and in ambient water of this restored basin to measure the efficiency of the rehabilitation program on quality of endogenous fish. A simultaneous parallel sampling program was also commenced for the other two basins of LM. The obtained results revealed that the concentration level of the studied metals in each of the muscles of the fish and the ambient water of the restored MB becomes now not only almost alike the metal levels of the corresponding compartments of the other two basins of LM, but also they became at concentration levels lay below those of their counterpart permissible limits in fish and water recommended by national and international standards. Health risk assessment indices: bioaccumulation factor (BAF), metal pollution index (MPI), estimated daily intake (EDI), hazardous index (HI), and relative risk (RR) for the present case were estimated, assessed, and subsequently evaluated. All are referring to a fact that MB is currently in good environmental condition and producing safe fish for human consumption.

Automating a Festo Manufacturing Machine with an Allen-Bradley PLC

Industry equipment such as machinery utilising programmable logic controllers (PLCs) become outdated and obsolete over time. Support for older machines and controllers becomes limited and they become incompatible with new computer operating systems. In the end, they are no longer used by industry. However, obsolete machines can be refurbished and used for teaching or demonstration purposes. Hence, this paper presents the reconditioning of a Festo manufacturing machine by replacing the old Festo PLC with an Allen-Bradley PLC so that it’s compatible with the mini-industrial network in the mechatronics lab. The machine is a Festo MPS storage and retrieval station featuring three axis electromechanical gantries, a gripper, DC motors with encoder feedback, reed switches, and a pneumatic actuator. The I/O connections from these components to the old PLC is traced and a new interface to the Allen-Bradley PLC is established while keeping connectivity with the old Festo PLC for legacy control. This upgrade allows the machine to be used for student training in the automation courses. It also leaves an option for utilising the old Festo PLC if needed. A sample program has been developed to test and verify correct interfacing and operation of the Allen-Bradley PLC and Festo storage and retrieval station.

Code Transform Model Producing High-Performance Program

This paper introduces a novel transform method to produce the newly generated programs through code transform model called the second generation of Generative Pre-trained Transformer (GPT-2) reasonably, improving the program execution performance significantly. Besides, a theoretical estimation in statistics has given the minimum number of generated programs as required, which guarantees to find the best one within them. The proposed approach can help the voice assistant machine resolve the problem of inefficient execution of application code. In addition to GPT-2, this study develops the variational Simhash algorithm to check the code similarity between sample program and newly generated program, and conceives the piecewise longest common subsequence algorithm to examine the execution’s conformity from the two programs mentioned above. The code similarity check deducts the redundant generated programs, and the output conformity check finds the best-performing generative program. In addition to texts, the proposed approach can also prove the other media, including images, sounds, and movies. As a result, the newly generated program outperforms the sample program significantly because the number of code lines reduces 27.21%, and the program execution time shortens 24.62%.

In Faith and Practice: How Christian Doctoral Programs Address Religious Diversity Needs

Many Americans identify as religious or spiritual, with a large subset of these individuals identifying as Christian. However, many doctoral training programs neglect to incorporate religion and spirituality into their curricula in an intentional and integrated way. Past national research has documented that many professionals report feeling incompetent, ill-prepared, or reticent to attend to religion and spirituality matters in their teaching and practices. In contrast, Christian doctoral programs address this problem by intentionally hiring qualified faculty who can articulate a clear faith commitment, and through curricular offerings that emphasize the integration of faith and professional practice. By doing so, they offer an approach to doctoral education that incorporates an additional dimension in terms of secular professional diversity standards. Programs like the ones outlined in this article enhance their students’ and trainees’ competency by attending to religion and spirituality as diversity factors. Such programs also attend to religious diversity as an essential personal dimension of life for many clientele. Furthermore, the integration of faith with academic endeavors has the potential to deepen professional identity development and to offer the added layer of integrating occupation and spiritual vocation. Three sample programs are described in this article, highlighting how each doctoral program—one in clinical psychology, one in law, and one in business administration—distinctively practices Christian integration. The benefits of these programs to students and other professional training programs are also discussed.

Comparison of eDNA and visual surveys for rare and cryptic bromeliad-dwelling frogs

Surveys of rare or cryptic species may miss individuals or populations that are actually present. Despite the increasing use of environmental DNA (eDNA) analysis to survey species in ponds, rivers, and lakes, very few studies have attempted to use eDNA for the detection of species using very small water bodies such as those accumulated within plants. Our aim was to investigate the feasibility of an eDNA sampling method for detecting Crossodactylodes itambe, an endemic bromeliad-dwelling frog from a remote location in Brazil. We collected water samples from 19 bromeliads for which we had observational data from direct visual surveys. We compared occupancy estimated from direct observations with the results from quantitative real-time PCR based eDNA assays. For observational surveys, we used a single season occupancy model. We applied a novel Bayesian occupancy model to estimate occupancy from eDNA samples, as well as false positives and false negatives at different stages of the workflow. eDNA from bromeliad tanks provided reliable estimates, with very low error levels and improved detection when compared to detectability from direct observation. Estimated occupancies using eDNA and visual survey methods were similar. The method is feasible for species restricted to small water bodies and exposed to direct UV radiation, and particularly useful to survey remote locations and confirm species presence. eDNA analysis provides a viable alternative to destructive sampling of bromeliads or direct observation methods that require logistically challenging repeated observations. Therefore, eDNA methods may be widely applicable to sampling programmes of other amphibians that live in plants.

Permian source rocks of the onshore and nearshore Carnarvon Basin

A new sampling program of Permian potential source rocks was undertaken to improve knowledge of the Permian petroleum prospectivity in new parts of the Southern Carnarvon and inboard Northern Carnarvon Basins. Presented here are new Rock-Eval data from previously unsampled wells, drillholes and outcrop and new infill sampling between existing data points. Kerogen assemblages of selected intervals were also determined from palynofacies analysis or organic petrography, which suggests the good Permian source rocks are generally dominated by gas-prone kerogens. Possibly terrestrial-derived oil-prone kerogen can also be frequently found in thin intervals of the upper Permian and more rarely in lower Permian in the onshore northern Carnarvon Basin.

Sample-size requirements for accurate length-frequency distributions of mesophotic reef fishes from baited remote underwater stereo video

Accurate descriptions of size structure are important for adaptive management of marine fish populations subject to anthropogenic and environmental pressures. This requires monitoring programs that can measure the length of enough individuals within each focal fish population. This study assessed the number of length measurements required to accurately describe the size structure in a range of common mesophotic demersal fish species observed from baited remote underwater stereo video (stereo BRUV) sampling programs. Here, we use a resampling approach from an empirical length dataset collected as a part of ongoing monitoring efforts to characterize mesophotic reef fish assemblages across the continental shelf of eastern and southern Tasmania, Australia. The results suggest that, on average, between 60 and 120 individuals length measurements are needed from at least 20 to 2000 independent deployments to be 95% confident that samples reflected the “real” size structure of fishes captured using stereo BRUVs. It is important to note that the “real” size-structure of each fish species here is unknown but was parameterized by pooling all measurements across the stereo BRUV dataset. It should also be noted that the exact number of length measurements differs across species, with some less abundant species requiring substantial sampling effort. This study helps to inform initial sampling requirements for length measurements for monitoring programs using stereo BRUVs. It provides a methodology that can aid researchers to further refine the overall sampling effort for future fisheries and marine park monitoring applications.

High-Resolution Sampling of a Broad Marine Life Size Spectrum Reveals Differing Size- and Composition-Based Associations With Physical Oceanographic Structure

Observing multiple size classes of organisms, along with oceanographic properties and water mass origins, can improve our understanding of the drivers of aggregations, yet acquiring these measurements remains a fundamental challenge in biological oceanography. By deploying multiple biological sampling systems, from conventional bottle and net sampling to in situ imaging and acoustics, we describe the spatial patterns of different size classes of marine organisms (several microns to ∼10 cm) in relation to local and regional (m to km) physical oceanographic conditions on the Delaware continental shelf. The imaging and acoustic systems deployed included (in ascending order of target organism size) an imaging flow cytometer (CytoSense), a digital holographic imaging system (HOLOCAM), an In Situ Ichthyoplankton Imaging System (ISIIS, 2 cameras with different pixel resolutions), and multi-frequency acoustics (SIMRAD, 18 and 38 kHz). Spatial patterns generated by the different systems showed size-dependent aggregations and differing connections to horizontal and vertical salinity and temperature gradients that would not have been detected with traditional station-based sampling (∼9-km resolution). A direct comparison of the two ISIIS cameras showed composition and spatial patchiness changes that depended on the organism size, morphology, and camera pixel resolution. Large zooplankton near the surface, primarily composed of appendicularians and gelatinous organisms, tended to be more abundant offshore near the shelf break. This region was also associated with high phytoplankton biomass and higher overall organism abundances in the ISIIS, acoustics, and targeted net sampling. In contrast, the inshore region was dominated by hard-bodied zooplankton and had relatively low acoustic backscatter. The nets showed a community dominated by copepods, but they also showed high relative abundances of soft-bodied organisms in the offshore region where these organisms were quantified by the ISIIS. The HOLOCAM detected dense patches of ciliates that were too small to be captured in the nets or ISIIS imagery. This near-simultaneous deployment of different systems enables the description of the spatial patterns of different organism size classes, their spatial relation to potential prey and predators, and their association with specific oceanographic conditions. These datasets can also be used to evaluate the efficacy of sampling techniques, ultimately aiding in the design of efficient, hypothesis-driven sampling programs that incorporate these complementary technologies.

Population structure of <i>Paralonchurus brasiliensis</i> (Steindachner, 1875) (Perciformes: Sciaenidae) in the coast of Sergipe, northeastern Brazil

Paralonchurus brasiliensis is one of the most common fish species found in the bycatch of shrimp trawling in Sergipe. We analyzed its population structure through four sampling programs, two using hired shrimp trawlers (2013-2014 and 2017-2018) and two from the commercial shrimp fleet (2015-2016 and 2018-2019). The codend mesh size varied among programs (18, 20, and 21 mm). All specimens of P. brasiliensis were identified and had their standard and total lengths (SL and TL; cm) and their total weight (TW; g) measured. A total of 1509 specimens were collected measuring 4.7-22.9 cm TL and 0.4-114.8 g. About 67% of all specimens were below the length at first maturity (14.7 cm). The length-length and weight-length relationships estimated were TL=1.141+1.176×SL and TW=0.00270×TL3.391, respectively. The recruitment probably occurs in September-October and May. This species appeared to be more abundant at 15 m, and larger individuals were found at 5 m. Although P. brasiliensis is frequently caught in shrimp fisheries, it is not reported in catch statistics. Here, for the first time, we have documented its presence in Aracaju’s market under the categories ‘miunça’ and ‘pescadinha’ (97% adults), with the largest specimens landed and commercialized as ‘pescadinha’ together with other species. Smaller individuals are probably discarded by commercial fishers. The low selectivity of the fishing gear used leads to the capture of many juveniles, which may impact the population dynamics of this species as well as other species found in the same fishing ground.

Modeling of Trihalomethanes in Benha Water Supply Network (Dept.P)

The presence of total trihalomethane (THMs) in Benha Water Supply Network (WSN) is determined and evaluated in this research. Six sampling points located an Different distances from the main water reservoirs, were selected in BWSN in order to follow the evolution | THMs. The gained information from the monthly sampling and laboratory analysis program was used to formulate relationship of total trihalomethanes (THMs) concentration in terms of initial chlorine dose, total organic carbon, bromide ion concentration, contact time, temperature, algae. and pH. To define the accuracy level of the developed model. the predicted (Mx concentrations from the developed model were compared to actual TUMS concentrations measured during the monitoring period. The developed mathematical model and software (EPANET), which was used to determine water age. were used to predict THMs concentrations throughout BWSN. The results showed close agreement between the measured and calculated THMs concentration. Hence the difference in the average calculated values and measured values during the study period ranged from (4.6% to 9.9 %). The model can provide reasonable results for most of the functioned variables and can be used to have a rapid assessment ol'llie trihalomethanes formation.

Characterising sediment physical property variability for bench-scale dewatering purposes

A field sampling programme was undertaken to assess the variability of physical characteristics of contaminated sediments in a large (160 ha) effluent stabilisation lagoon. The objective of this paper is to use this ‘field lab’ as a basis for comparing different sampling techniques (i.e. discrete and composite) for remediation-based evaluations (i.e. sediment volume estimates and bench-scale dewatering studies). The distribution of sediment thickness measured throughout the lagoon by gravity core sampling is presented for context. Selected gravity core sediment samples are evaluated with respect to physical property (water/solids content, bulk density and particle size) variability in both the vertical (i.e. within a single gravity core) and spatial directions (among gravity cores). Composite samples are created by way of homogenisation of a single entire gravity core to compare their properties to the discrete and average physical properties of a nearby gravity core. Vacuum-based samples are also compared to gravity core samples in terms of particle size. It is demonstrated that by understanding sediment variability, composite samples can be shown to be an efficient method of obtaining representative samples. When large samples for dewatering trials are required, vacuum sampling can produce samples with similar mean particle size to discrete and composite samples.

NVESTIGATION ON TUNA FISHERIES ASSOCIATED WITH FISH AGGREGATING DEVICES (FADs) IN INDONESIA FMA 572 AND 573

The use of anchored fish aggregating devices (a-FADs) in the Indonesia-Indian Ocean has increased rapidly. Since 2004, the Government of Indonesia has issued various FAD related fisheries regulations; however, its implementation was difficult, largely due to the lack of such information. As an endeavor to improve the management of tuna fisheries associated with FADs in this area, an Indonesia–Australia research collaboration project conducted a port sampling program from November 2013 to December 2015 in three key fishing ports in the western Indonesia, i.e., Pelabuhanratu (West Jawa), Muara Padang (West Sumatera), and Bungus (West Sumatera). Data were collected through daily enumeration and interviews with skippers, which consisted of catch, trip duration, biological data, and number of FADs visited. These data were analyzed to estimate catch rate, success rate, and length frequency distribution. The success rate of hand line/trolling line (HL/TR) at Muara Padang showed much lower than that at Pelabuhanratu. This may be due to more a-FADs or higher density in the Padang region, competing with purse seine (PS) boats operating in the same area, than those in the Pelabuhanratu region. The species composition caught by HL/TR and PS associated a-FADs in Indonesian FMA 572 and 573 include skipjack (SKJ, Katsuwonus pelamis), yellowfin (YFT, Thunnus albacares) and bigeye tuna (BET, T. obesus). A large proportion of the SKJ, YFT and BET caught at both Indonesian FMA 572 and 573 were juvenile fish, below the reported length at maturity (Lm) for those species.

The forgotten feeding ground: patterns in seasonal and depth-specific food intake of adult cod Gadus morhua in the western Baltic Sea.

This study presents the diet composition of western Baltic cod Gadus morhua based on 3150 stomachs sampled year-round between 2016 and 2017 using angling, gillnetting and bottom trawling, which enhanced the spatio-temporal coverage of cod habitats. Cod diet composition in shallow areas (<20 m depth) was dominated by benthic invertebrate species, mainly the common shore crab Carcinus maneas. Compared to historic diet data from the 1960s and 1980s (limited to depth >20 m), the contribution of herring Clupea harengus decreased and round goby Neogobius melanostomus occurred as a new prey species. Statistical modelling revealed significant relationships between diet composition, catch depth, fish length and season. Generalized additive modelling identified a negative relationship between catch depth and stomach content weight, suggesting reduced food intake in winter when cod use deeper areas for spawning and during peak summer when cod tend to avoid high water temperatures. The results of this study highlight the importance of shallow coastal areas as major feeding habitats of adult cod in the western Baltic Sea, which were previously unknown because samples were restricted to deeper trawlable areas. The results strongly suggest that historic stomach analyses overestimated the role of forage fish and underestimated the role of invertebrate prey. Eventually, this study shows the importance of a comprehensive habitat coverage for unbiased stomach sampling programmes to provide a more reliable estimation of top predator diet, a key information for food web analyses and multispecies models.

Long-term changes of ichthyoplankton communities in an Iberian estuary are driven by varying hydrodynamic conditions

Abstract Ichthyoplankton assemblages are key components of estuaries worldwide, playing a vital role as nurseries for fish larvae. Nonetheless, estuaries can be highly affected by ongoing climate change. Impacts of climate variability on ichthyoplankton assemblages will have consequences for marine pelagic food webs and fish populations biology, namely recruitment. This study aims to investigate the influence of environmental variability on an interannual abundance of ichthyoplankton assemblages of the Mondego estuary (Portugal). For this, an ichthyoplankton sampling programme of 13 years (2003–2015) along six distinct sampling stations was analysed to evaluate spatial, seasonal and interannual changes of ichthyoplankton distribution over periods of wet, regular and dry conditions. The ichthyoplanktonic community was dominated by Pomatoschistus spp. across all seasons and conditions, with higher larval abundances during summer and spring. Main changes were related to species seasonality and phenology as well as an increase in the number of marine species during extreme events. The larval fish community showed a strong relationship with the regional and local environment over the study, presenting a distinct yet highly variable structure during the 2009–2013 period. Reported changes will likely trigger major changes in species dominance and abundance, with clear ecological and socio-economic implications.

Pilot implementation of a home-care programme with chlamydia, gonorrhoea, hepatitis B, and syphilis self-sampling in HIV-positive men who have sex with men

BackgroundNot all men who have sex with men (MSM) at risk for sexually transmitted infections (STIs) and human immunodeficiency virus (HIV) infection currently receive sexual healthcare. To increase the coverage of high-quality HIV/STI care for MSM, we developed a home-care programme, as extended STI clinic care. This programme included home sampling for testing, combined with treatment and sexual health counselling. Here, we pilot implemented the programme in a hospital setting (HIV-positive MSM) to determine the factors for the successful implementation of STI home sampling strategies.MethodsHealthcare providers from the HIV hospital treatment centre (Maastricht) were invited to offer free STI sampling kits (syphilis, hepatitis B, [extra]genital chlamydia and gonorrhoea laboratory testing) to their HIV-positive MSM patients (March to May 2018). To evaluate implementation of the program, quantitative and qualitative data were collected to assess adoption (HIV care providers offered sampling kits to MSM), participation (MSM accepted the sampling kits) and sampling-kit return, STI diagnoses, and implementation experiences.ResultsAdoption was 85.3% (110/129), participation was 58.2% (64/110), and sampling-kit return was 43.8% (28/64). Of the tested MSM, 64.3% (18/28) did not recently (< 3 months) undergo a STI test; during the programme, 17.9% (5/28) were diagnosed with an STI. Of tested MSM, 64.3% (18/28) was vaccinated against hepatitis B. MSM reported that the sampling kits were easily and conveniently used. Care providers (hospital and STI clinic) considered the programme acceptable and feasible, with some logistical challenges. All (100%) self-taken chlamydia and gonorrhoea samples were adequate for testing, and 82.1% (23/28) of MSM provided sufficient self-taken blood samples for syphilis screening. However, full syphilis diagnostic work-up required for MSM with a history of syphilis (18/28) was not possible in 44.4% (8/18) of MSM because of insufficient blood sampled.ConclusionThe home sampling programme increased STI test uptake and was acceptable and feasible for MSM and their care providers. Return of sampling kits should be further improved. The home-care programme is a promising extension of regular STI care to deliver comprehensive STI care to the home setting for MSM. Yet, in an HIV-positive population, syphilis diagnosis may be challenging when using self-taken blood samples.

Establishment of environmental sampling baseline of a nuclear facility using large geometry-secondary ion mass spectrometry (LG-SIMS)

The Korea Institute of Nuclear Nonproliferation and Control (KINAC) operates the “environmental sampling program” which includes the collection, screening, analysis of samples, and evaluation of the resulting data. The goal of this project is to set up the nuclear material baseline in domestic nuclear facility, so that any future sampling results can then be evaluated using the facility baseline as a reference. For this purpose, 18 swipe samples from three points of the nuclear facility were collected, and the screening test using monochromatic micro-focusing X-ray fluorescence was conducted, followed by particle analysis of the collected swipe samples. The particle analysis was conducted using large geometry-secondary ion mass spectrometry. The MMXRF map was effectively applied for making a decision to select particles from samples for further particle analysis. Based on the results of particle analysis, the baseline for each selected point of the nuclear facility was established, and these datasets will be used as reference for verifying the absence of undeclared activities at the facility in the future. The results showed that the established baseline of the nuclear facility was consistent with the declared activities.

Two new species of the genus Ancyronyx Erichson, 1847 from the island of Negros, Philippines (Insecta, Coleoptera, Elmidae)

Two new Elmidae species from the Visayan island of Negros, Philippines, Ancyronyx berghaueri sp. n. and Ancyronyx negrosensis sp. n., are described utilizing an integrative taxonomic approach. The new species are characterized by the color of the femora, body size, and the male and female genitalia. Their habitus and diagnostic characters are described and illustrated in detail with microscopic images and digital line drawings. The morphological species delimitation is supplemented by and congruent with COI and cob mtDNA data of several known species of Ancyronyx from the Philippines and Borneo. The material was obtained in connection with a student thesis, the sampling program of the Ateneo de Manila University Biodiversity Laboratory and from repositories. Specimens were collected at altitudes between 30 m and 800 m above sea level in pristine and slightly disturbed habitats. Their suitability as bioindicators and the use of cob sequences for integrative taxonomic studies are briefly discussed.