Progesterone Sponges Research Articles

The use of FSH and GnRH as alternative compounds to PMSG for springtime breeding of ewes

During early springtime, ewes were treated with a single injection of PMSG (500 IU) administered at the time of progesterone sponge withdrawal (Group I) or four, twice daily injections of FSH (2 mg each) administered from twelve hours before, to 24 hours after sponge withdrawal (Group II). One hundred μg of GnRH was administered 36 hours after sponge withdrawal in Group II. The two groups were compared with respect to estrous display, conception rate and other reproductive parameters. There were no significant differences (p>.05) between the two treatment group in any of the reproductive parameters measured. The occurrence of estrus during the first 96 hours after synchronization was low ( X = 45% of all treated ewes). Conception of all treated ewes during this estrous period was also low ( X = 32% of all treated ewes). The potential use of FSH and GnRH in estrous synchronization regimes was discussed. Possible reasons for the poor estrous and conception rates in present experiment were also considered.

Ovarian response, ova recovery and fertility in merino ewes superovulated either during the luteal phase of their oestrous cycle or after intravaginal progestagen treatment

Five groups of merino ewes were treated with 1000 i.u. of pregnant mare serum gonadotropin (PMSG) as a single injection per ewe. Three of these groups received treatment on days 7,9 and 11 of their oestrous cycle. Oestrus was synchronized with 125 mg of prostaglandin F2 α (PG) given two days after PMSG. Oestrus in the other two groups was synchronized by intravaginal progesterone sponges inserted for 14 days. In one group, the sponges were inserted nine days after oestrus onset. In the other group the stage of the oestrous cycle was unknown. In both these groups, PMSG was given a day prior to sponge removal. No significant differences were recorded for either the mean numbers of corpora lutea, unovulated follicles or ova recovery between the five groups. However, progestagen synchronized ewes yielded significantly more fertilized ova (p < 0.05) than PG synchronized ewes.

Artificial insemination and natural service at a predetermined time in cyclic sheep treated with ‘SC-9880’-progesterone sponges

SUMMARYResults are given of an experiment in which 61 cyclic Finnish Landrace × Dorset Horn ewes were synchronized for oestrus by intravaginal treatment for 14 days using sponges impregnated with 30 mg ‘Cronolone’ (9a-fluro-11-hydroxy-17a-acetoxy-progesterone; SC-9880, G. D. Searle) and 400 mg progesterone. Groups of 31 and 30 sheep were inseminated twice, either artificially or naturally, at predetermined intervals of 48 and 58 hr after sponge withdrawal. All 61 ewes were in oestrus within 48 hr of removing sponges; 26 of 30 (87%) artificially inseminated and 29 of 31 (94%) hand-mated sheep conceived at the controlled oestrus. The incidence of multiple births was not significantly affected by the method of breeding.