AbstractThe sulfated polysaccharide fucoidan was extracted from brown algae Turbinaria conoides using hot water and purified by anion‐exchange chromatography. The biochemical and monosaccharide composition of purified fucoidan was studied by colorimetric assays and Gas Chromatography Mass Spectrometry (GCMS). The purity of fucoidan was confirmed by agarose gel electrophoresis and the structure was characterized by using spectroscopic techniques such as Fourier transform infrared spectroscopy (FT‐IR) and solid‐state nuclear magnetic resonance (NMR) techniques. Further, the bioactivity properties of fucoidan such as antioxidant, anticoagulant and anti‐inflammatory were evaluated. The results showed profound antioxidant and anti‐inflammatory activities of fucoidan. The anti‐coagulant activity of fucoidan demonstrated striking inhibition of intrinsic and extrinsic coagulants involved in coagulation pathway.