Recent COVID-19 online dating research have investigated the strategies people used during the pandemic dating, however, the relationship between displaying health cues on online dating profiles and making online dating decisions has been understudied. A 2 (Profile Owner/Viewer’s Gender: Male vs. Female) x 2 (Smoking Status: Non-Smoker vs. Smoker) x 2 (COVID-19 Vaccination status: Not Vaccinated vs. Vaccinated) between-subjects online experiment (N = 250) was conducted to examine the impact of gender, smoking, and COVID-19 vaccination status on online dating decision. Results showed non-smoking profile owners were more likely to receive the video chat invitation compared with smoking profile owners. Male profile viewers were more likely to send message, video chat, meet offline, make short-term and long-term dating decisions toward profile owners compared with female profile viewers. Vaccinated females and unvaccinated males were more likely to receive the offline meeting invitation. An online dating profile owner’s perceived intelligence and perceived similarity would vary as a function of his/her vaccination status and smoking status. In addition, loneliness moderated the relationship between smoking/vaccination status and long/short term dating decisions. This study contributed to the literature of online dating and provided a roadmap for the online dating website designers.