Professor Of Biology Research Articles

Future leader to watch – Nathan Harmston

ABSTRACTFirst Person is a series of interviews with the authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Nathan Harmston is last author on ‘ The importance of considering regulatory domains in genome-wide analyses ­– the nearest gene is often wrong!’, published in BiO. Nathan is Assistant Professor of Computational Biology at Yale NUS College, Singapore, investigating the rules and features of how gene expression is regulated in both development and disease.

More Pieces of the Puzzle: New Insights into Azoxystrobin Exposures and Neurotoxicity.

More Pieces of the Puzzle: New Insights into Azoxystrobin Exposures and Neurotoxicity.

Geobiology

Lourens Baas Becking (1895-1963) was a Dutch plant physiologist, trained in the Botanical Laboratory of Utrecht University. After graduating in 1919, he worked in America at Stanford University, where he obtained his Doctor’s degree in 1921. From 1928, he was Herzstein Professor of Biology and Director of the Jacques Loeb Physiological Laboratory at the Hopkins Marine Station in Palo Alto. In 1931, he became Professor of General Botany at the University of Leiden. There, he and his staff and students continued to work on the research of microorganisms under extreme saline conditions. In 1939, he was appointed Director of the institutes of the Botanic Garden at Buitenzorg (Bogor) in the Dutch East Indies (Indonesia). In May 1940, when the war broke out, he was in Leiden to retire from his professorship. The war prevented his return to his family and the institutes in the East Indies. Baas Becking made several failed attempts to escape to England. These resulted in imprisonments by the German occupying authorities in Scheveningen (1940-1941) and in Utrecht and the German Zuchthaus in Siegburg (1944-1945). An ordeal that he barely survived due to the inhuman situation in the penitentiary and typhus. In July and August 1944, as a prisoner of the German Kriegsmarine in Utrecht, he wrote in seven weeks a manuscript of Geobiology, an essay on the relationship between living organisms and the earth. It was an update of his earlier ideas. Baas Becking had been inspired by Lawrence Henderson’s The Fitness of the Environment (1913), Victor Moritz Goldschmidt's Der Stoffwechsel der Erde (1922) and Grundlagen der quantitativen Geochemie (1933), Alfred J. Lotka’s Elements of Physical Biology (1924) and Vladimir Vernadsky’s La Géochimie (1924). They were with Frank W. Clarke’s The Data of Geochemistry (1916), sources for his perception of The Universality of Life in 1927, which integrated Vernadsky’s concepts of biosphere and geosphere. Long before James Lovelock and Lynn Margulis defined the Gaia hypothesis in the early 1970s, Baas Becking discussed Gaia or Life and Earth in his inaugural address in 1931. In this tract he also succinctly summarised the ubiquity hypothesis, borrowed from the work of Martinus Beijerinck, as “Everything is everywhere, but the Milieu selects.” The biological “law” was further elaborated in Geobiologie of inleiding tot de milieukunde (1934, English version 2016, Baas Becking’s Geobiology). In the Utrecht prison Baas Becking wrote his scientific testament. In the ten years since the publication of Geobiologie, he “wished to do justice to the work that was performed in Leiden by so many workers”, in an English textbook. With a limited access to scientific literature, he wrote the manuscript Geobiology in a ledger in a barely legible handwriting. The document reflected his vast biological knowledge and his idea of mutual dependence of vital-units (cells, tissues, organs, organism, communities), either of a parasitic, mutualistic or commensalistic character. This relationship was elaborated in his model of symbiosis. His description of the role of man in Geobiology is a personal complaint of a geobiologist over the disastrous treatment of the earth by man. With his concept of “dissipation”, he introduced a material analogue for “the entropy lowering capacity of living systems”. It summarised his conviction that the human intellect and life condition were attributes of free will. Although Geobiology (1944) remained unfinished and had major gaps, it still is an inspiring memoir of a scientist who records his enlightened vision on the relationship between life and earth. In this issue of Geochemical Perspectives the manuscript of Geobiology is integrally transcribed, annotated, edited and introduced by Dr. Alexander J.P. Raat, who graduated in 1974 in Leiden as a plant physiologist. The transcript is published with the original illustrations. A sketch of Baas Becking’s life and works is part of the introduction. The annotation and introduction refer to many of his published and unpublished studies. Among these is an unpublished, further updated and revised version of Geobiology, which he completed in 1953 in Australia.

Neuroblastoma in childhood and its potential viral involvement: A webinar by the Paediatric Virology Study Group

Neuroblastoma represents the most common and lethal solid tumour of early childhood. In view of variations in genetic elements, MYCN amplification is certainly the most prominent genetic factor occurring in 1/4 or 1/5 of children with neuroblastoma; however, overall, the pathogenesis of neuroblastoma remains to be resolved. Rare, sporadic infections with Epstein‑Barr, hepatis C virus and varicella‑zoster virus have been detected in children with neuroblastoma, while the presence of BK virus was initially claimed, but later falsified as a triggering factor for the development of high‑risk neuroblastoma. The proposed model by Professor Ugo Rovigatti, Professor of Molecular Biology at the University of Florence in Italy, is based upon infection with micro‑foci inducing virus and its potential tumorigenic role as trigger of i) high and persistent inflammation; ii) chromothripsis and genetic instability; and iii) <em>in vitro</em> cell transformation and <em>in vivo</em> carcinogenesis. Modelling is still under‑utilized and under-developed in clinical virology and cancer research; however, it is expected to play a significant role in the future, aiming to elucidate the cancer enigma. This article is based on a webinar on neuroblastoma in children, which was organised virtually on December 12, 2020, by the Institute of Paediatric Virology.

The “Rs” of Contemporary Biology Education

Editorial| March 01 2022 The “Rs” of Contemporary Biology Education Gordon Uno Gordon Uno GORDON UNO (guno@ou.edu) is a professor of plant biology at the University of Oklahoma, Norman, OK 73019. Search for other works by this author on: This Site PubMed Google Scholar The American Biology Teacher (2022) 84 (3): 119. https://doi.org/10.1525/abt.2022.84.3.119 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Gordon Uno; The “Rs” of Contemporary Biology Education. The American Biology Teacher 1 March 2022; 84 (3): 119. doi: https://doi.org/10.1525/abt.2022.84.3.119 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentThe American Biology Teacher Search School days, school days Dear old Golden Rule days Reading and ‘riting and ‘rithmetic Taught to the tune of the hick’ry stick —(Cobb and Edwards, 1907) These phrases from the old song “School Days” proclaimed the importance of three fundamental “Rs” of primary education: reading, writing, and arithmetic, with the hickory stick providing “incentive” to learn. Fast forward 115 years, American instructors—now appropriately without the stick—feel challenged to help students learn ever-increasing amounts of complex biological content and prepare students for high-stakes standardized exams, potential science careers, and decision-making as science-literate citizens. Here, I offer 10 Rs that may serve as a foundation for contemporary biology education. Modern biology should include the three original Rs, which are still essential for all students. (1) Reading, of course, relates to interpreting and understanding (not memorizing) the results and explanations of science research found in textbooks, primary literature, and written news reports.... You do not currently have access to this content.

Lessons in chromatin organization and gender equity in research: an interview with Susan Gasser.

In this interview, Professor Susan Gasser speaks with Storm Johnson, commissioning editor for Epigenomics, on her research on genome stability, epigenetic regulation and chromatin organization, as well as her work supporting women in research. Susan Gasser completed her BA at the University of Chicago, with an honors thesis in biophysics, and her PhD in biochemistry at the University of Basel in 1982, with Gottfried Schatz. She was a postdoc with Ulrich Laemmli at the University of Geneva, which initiated her career-long interest in chromosomes and chromatin structure. She established her own laboratory at the Swiss Institute for Experimental Cancer Research (ISREC) in 1986, focusing on chromatin organization in budding yeast, combining genetics, microscopy and biochemical approaches to understanding silent chromatin and telomeres. In 2001, she was named professor of molecular biology at the University of Geneva and expanded her laboratory's pioneering use of high-resolution time-lapse fluorescence microscopy to study single locus dynamics in the nucleus. From 2004 to 2019, Susan was the Director of the Friedrich Miescher Institute for Biomedical Research in Basel, where she also led a research group until the end of 2020. In Basel, she extended her research interests into heterochromatin in Caenorhabditiselegans. Her laboratory identified the mechanisms that position tissue-specific genesin the nuclei ofembryos and ofdifferentiated tissues, combining high throughput molecular analyses with cell biology to determine structure-function relationships in chromatin. Since January 2021, Susan Gasser has been professor invité at the University of Lausanne and Director of the ISREC Foundation, where she is helping shape the new Agora Institute of Translational Cancer Research. She was elected to the Académie de France, Leopoldina, European Molecular Biology Organization (EMBO), American Association for the Advancement of Science and Swiss Academy of Medical Sciences, and she received the French National Institute of Health and Medical Research (INSERM) International Prize in 2011, the Federation of European Biochemical Societies | EMBO Women in Science Award in 2012, the Weizmann Institute Women in Science Award in 2013 and honorary doctorates from the University of Lausanne, the University of Fribourg and Charles University in Prague. In Switzerland, she was the recipient of the Friedrich Miescher Award, the National Latsis Prize and the Otto Naegeli Award for the promotion of medical research. She participates in numerous review boards and advisory committees in Switzerland, across Europe and in Japan; she currently serves on the governing board of the Swiss Federal Institutes of Technology and the Swiss Science Council. From 2000 to 2004, she was vice chairperson, then chairperson of the EMBO Council. Susan led the Gender Committee of the Swiss National Science Foundation from 2014 to 2019 and initiated the Swiss National Science FoundationPrima program for the Promotion of women in academia. She has actively promotedthe careers of women scientists in Europe and Japan.

A different kind of smart: What pathologists can learn from an octopus: Cephalopods may depend on a vastly different form of perception, but their intelligent problem-solving can supply plenty of relatable lessons.: Cephalopods may depend on a vastly different form of perception, but their intelligent problem-solving can supply plenty of relatable lessons.

A different kind of smart: What pathologists can learn from an octopus: Cephalopods may depend on a vastly different form of perception, but their intelligent problem-solving can supply plenty of relatable lessons.: Cephalopods may depend on a vastly different form of perception, but their intelligent problem-solving can supply plenty of relatable lessons.

Scientific Societies Team Up to Foster a Diverse STEM Workforce

The Science, Technology, Engineering, and Math (STEM) communities have a long history of exclusion and underrepresentation of women, African American, Latinx, American Indian and LGBTQIA+ students. In order for our STEM enterprise to be truly equitable, everyone that wants to become a scientist must have an equitable opportunity to do so, regardless of their gender, ethnicity or sexual orientation. In the movement toward equity, the demographic diversity of the STEM workforce must mirror that of the general population. STEM workforce diversity can accelerate innovation in scientific disciplines, and, if coupled with systemic cultural equity, can also support a STEM enterprise where everyone can thrive. Dr Verónica A. Segarra, Interim Chair and Assistant Professor of Biology at High Point University, has been exploring how scientific societies could help their disciplines be more equitable. Her efforts have helped to establish numerous alliances and collaborations among societies and diversity-focused organisations, with the mission of building a more diverse and inclusive STEM workforce.

Editorial 2022

Editorial 2022

Dieter Eckstein, 1939-2021 and his rich legacy of dendrochronology in Slovenia and the world

Prof. Dr. Dieter Eckstein (1939-2021) was a leading scientist, teacher, mentor, leader, promoter and motivatorin the field of dendrochronology and wood biology. After graduating in wood science and receiving a PhD indendrochronology, he was professor of wood biology at the University of Hamburg. From 1995-2004, he was Director of the Department of Wood Biology, University of Hamburg, and of the Institute of Wood Biology and Wood Protection at the Federal Research Centre for Forestry and Forest Products in Hamburg, Germany. His work had a decisive influence on the development of wood anatomy, wood biology and dendrochronology and his laboratory was a reference point for dendrochronology worldwide. He supported dendrochronologists throughout Europe and around the world in their pioneering work to establish dendrochronology laboratories and develop dendrochronology in numerous countries, including Slovenia.

Curing Incurable Cancer

Novel Approaches in Cancer Study Curing Incurable Cancer Doug Dix* Department of Health Science and Nursing, USA *Corresponding author: Doug Dix, Professor of Biology and Medical Technology, Department of Health Science and Nursing, West Hartford, Connecticut-06117, USA Submission: December 13, 2021 Published: December 20, 2021 DOI: 10.31031/NACS.2021.06.000645 ISSN:2637-773XVolume6 Issue4

Baltimore Wins Lasker-Koshland Award.

The Albert and Mary Lasker Foundation has bestowed its prestigious Lasker-Koshland Award for Special Achievement in Medical Science upon David Baltimore, PhD, president emeritus and distinguished professor of biology at the California Institute of Technology (Caltech) in Pasadena, and 1975 Nobel Laureate in Physiology or Medicine, calling him "one of the premier biomedical scientists of the last five decades."

"But what are you doing to help them learn?" Reflections on a Career Teaching Human anatomy and Physiology

This narrative study tells the teaching and learning story of the recently retired Dr. Peggy LePage, a longtime member of the Human Anatomy and Physiology Society with a career as a community college anatomy and biology professor spanning nearly three decades. Drawn from a lengthy narrative interview, this paper describes Dr. LePage’s background as a nontraditional student and explores her resilience as a woman in science, technology, engineering, and mathematics (STEM). When Dr. LePage neared a breaking point early in her career, an encounter with a well-known advocate for active learning in science classrooms transformed her pedagogy. She also became involved with HAPS and never looked back. Narrative passages from her interview emphasize Dr. LePage’s persistence in STEM, her shift toward a teacher as learner mentality, and the benefits of learning communities for anatomy and physiology instructors.

Mission net-zero America: The nation-building path to a prosperous, net-zero emissions economy

Jesse D. Jenkins is an assistant professor at Princeton University in the department of mechanical and aerospace engineering and the Andlinger Center for Energy and the Environment. He is a macro-scale energy systems engineer with a focus on the rapidly evolving electricity sector and leads the Princeton ZERO Lab, which focuses on improving and applying optimization-based energy systems models to evaluate low-carbon energy technologies and generate insights to guide policy and planning decisions. Jesse earned a PhD and SM from the Massachusetts Institute of Technology and was previously a postdoctoral environmental fellow at Harvard University. Erin N. Mayfield is an assistant professor at the Thayer School of Engineering at Dartmouth College focusing her research on sustainable-systems engineering and public policy. She develops and applies multi-objective computational models that integrate techno-economic, environmental, and social equity objectives to inform energy and industrial infrastructure transitions. Prior to her academic research career, her work with other organizations included regulatory assessments of environmental rule-makings, hazardous waste remediation in environmental justice communities, and ecosystem services valuation related to petrochemical and pesticide contamination. Erin has a PhD in engineering and public policy from Carnegie Mellon University and was previously a postdoctoral scholar at Princeton University. Eric Larson is a senior research engineer at Princeton University leading the Energy Systems Analysis Group in the Andlinger Center for Energy and the Environment. He holds courtesy appointments with the High Meadows Environmental Institute and the Center for Policy Research on Energy and the Environment in the School of Public and International Affairs. His research, intersecting engineering, environmental science, economics, and public policy, aims to identify sustainable, engineering-based solutions to major energy-related problems and to help inform public and private decision-making in the U.S. and elsewhere. Eric has a PhD in mechanical engineering from the University of Minnesota. Stephen Pacala is the Frederick D. Petrie professor in ecology and evolutionary biology at Princeton University and director of the Princeton Carbon Mitigation Initiative. His research interests are in the design and testing of mathematical models to investigate interactions between greenhouse gases, climate, and the biosphere and to predict their effects on local and global ecosystems. He is a member of the U.S. National Academy of Sciences, the American Association for the Advancement of Science, and the American Academy of Arts and Sciences. Steve has a PhD in biology from Stanford University. Chris Greig is the Theodora D. and William H. Walton III senior research scientist in the Andlinger Center for Energy and the Environment at Princeton University. His research interests lie in energy transitions, economics, and policy; capital project investment decision-making and implementation; and carbon capture, utilization, and storage. Chris has a PhD in chemical engineering from the University of Queensland and is a fellow of the Australian Academy of Technology and Engineering. His academic career was preceded by 25 years in senior project and executive roles in the resources and energy sectors.

Resilience forms pact with Harvard

National Resilience, a company launched last year to advance disruptive technologies in drug manufacturing, is widening its mandate. It has signed a 5-year R&D partnership with Harvard University to develop novel biologics, vaccines, cell and gene therapies, and other complex medicines. A $30 million investment from Resilience will fund faculty research, coordinated by Harvard’s office of technology development, into therapeutic and manufacturing technologies with an eye toward commercialization. The partners claim to have identified one technology to culture millions of in vitro cells that behave like skeletal muscle stem cells for the treatment of skeletal muscle disorders. Resilience has formed a company, Circle Therapeutics, to license the technology, which was developed in the lab of Lee Rubin, a professor of stem cell and regenerative biology at Harvard. Resilience debuted last November as a venture-backed start-up with $800 million in the bank. Industry watchers initially viewed Resilience as a contract development

Editor Profile: Nektarios Tavernarakis.

In this special interview series, we profile members of The FEBS Journal editorial board to highlight their research focus, perspectives on the journal and future directions in their field. Nektarios Tavernarakis is Chairman of the Board of Directors at the Foundation for Research and Technology-Hellas (FORTH), and Professor of Molecular Systems Biology at the University of Crete Medical School, Greece. In 2020, he was elected Vice President of the European Research Council (ERC). He has served as Editorial Board Member of The FEBS Journal since 2018.

Cell scientist to watch – Prachee Avasthi

ABSTRACT Prachee Avasthi studied Molecular and Integrative Physiology at the University of Illinois at Urbana-Champaign. She received her PhD in neuroscience in 2009 from the lab of Wolfgang Baehr at the University of Utah for her work on the control of membrane protein trafficking in photoreceptors. Prachee then moved to Wallace Marshall's group at the University of California, San Francisco, for her postdoc, where she studied ciliary assembly and the regulation of ciliary length. She set up her lab at the University of Kansas Medical Center in 2015, and relocated to the Geisel School of Medicine at Dartmouth in 2020, where she is an Associate Professor of Biochemistry and Cell Biology. Her group investigates the biogenesis of cilia and the coordination of actin- and microtubule-based trafficking.

In Their Own Words: Nalini Nadkarni

Abstract In Their Own Words chronicles the stories of scientists who have made great contributions to their fields. These short histories provide our readers a way to learn from and share their experiences. We will publish the results of these conversations in the pages of BioScience and on our podcast, BioScience Talks (http://bioscienceaibs.libsyn.com). This history is with Nalini Nadkarni, professor of biology at the University of Utah.

An interview with Nobel Laureate David Baltimore, PhD.

In an online interview, Nobel Laureate David Baltimore, Ph.D., reflected on his contributions to biomedical science that have had a major influence on the fields of molecular biology, virology, cancer, and immunology. Dr. Baltimore is President Emeritus and Distinguished Professor of Biology at the California Institute of Technology. Among other notable works, he discovered the critical nuclear transcription factor NF Kappa B and the Rag1 and Rag2 proteins that rearrange adaptive immune cell receptors.&#x0D; His career path, he says, evolved naturally, as math and science came easily to him. As a high school student, he participated in a summer program at the Jackson Lab in Bar Harbor, Maine, where he says he came away feeling that experimental biology was exciting and rewarding.&#x0D; “That's where I discovered that the frontiers of science, were not so distant; that I could actually make a discovery that nobody else in the world knew about,” he says. &#x0D; And that he did. Independently, he and Howard Temin discovered the viral enzyme reverse transcriptase revising the canon of cellular information transfer. They published back-to-back papers in Nature demonstrating that this enzyme in virus particles could transcribe RNA to DNA. Both received a Nobel Prize for this work.&#x0D; In reflecting on his early experience evaluating how to work with recombinant DNA and how we should scientifically and safely approach gain of function research, he says, “We have to be very honest with ourselves about what might hold danger, and we have to control our instinct … to do anything we can to generate progress and understanding of life. …At the same time, we don’t want to hold back progress, and so there is a balancing.”&#x0D; Dr. Baltimore also discussed his optimism about vectored immunoprophylaxis as a strategy for prevention of HIV and his doubt that scalable strategies will be able to cure HIV. He also reflected on his philosophy for the training of young scientists and the successful training program that he developed at the Whitehead Institute.

The ultra-long study: a randomized controlled trial evaluating long-term GnRH downregulation prior to ART in women with endometriosis.

Does ultra-long downregulation with a GnRH agonist (triptorelin depot) in previously operated patients with endometriosis improve the rate of clinical pregnancy with positive fetal heart beat (CPHB) in the subsequent initiated fresh ART cycle? Ultra-long downregulation with a GnRH agonist prior to ART did not improve the rate of CPHB in the subsequent fresh ART cycle in previously completely operated patients but the trial was underpowered due to early termination. Administration of GnRH agonists for a period of 3-6 months prior to ART in women with endometriosis may increase the odds of clinical pregnancy. However, the quality of the studies on which this statement is based is questionable, so these findings need confirmation. A controlled, randomized, open label trial was performed between 1 June 2013 and 31 December 2016 (start and end of recruitment, respectively). Patients with prior complete laparoscopic treatment of any type or stage of endometriosis and an indication for ART were randomized (by a computer-generated allocation sequence) into two groups: the control group underwent ART stimulation in a classical long agonist protocol using preparation with oral contraceptives, the ultra-long group first underwent at least 3 months downregulation followed by a long agonist protocol for ART stimulation. The sample size was calculated to detect a superiority of the ultra-long downregulation protocol, based on the hypothesis that baseline CPHB rate in the control group of 20% would increase to 40% in the ultra-long group. For a power of 20% at a significance level of 5%, based on two-sided testing, including 5% of patients lost to follow-up, the necessary sample size was 172 patients (86 per group). This trial was conducted at the Leuven University Fertility Center, a tertiary care center for endometriosis and infertility, and a total of 42 patients were randomized (21 in the control group and 21 in the ultra-long group). Baseline characteristics were similar in both groups. The primary outcome studied-CPHB after the initiated ART treatment-did not differ and was 25% (5/20) in the control group, and 20% (4/20) in the ultra-long group (P > 0.999; relative risk (RR) 1.25, 95% CI 0.41-3.88). Cumulative (fresh + associated frozen) CPHB rates were also similar in the control versus ultra-long group (8/20, 40% vs 6/20, 30%, P = 0.7411; RR = 1.33, 95% CI 0.57-3.19). When other secondary outcomes were compared with the ultra-long group, patients from the control group had a shorter duration of stimulation (mean 11.8 days (SD ± 2.4) versus 13.2 days (SD ± 1.5), P = 0.0373), a lower total dose of gonadotrophins used (mean 1793 IU/d (SD ± 787) vs 2329 (SD ± 680), P = 0.0154), and a higher serum estradiol concentration (ng/ml) at the end of ovarian stimulation on the day of ovulation triggering or cycle cancellation (mean1971 (SD ± 1495) vs 929 (± 548); P = 0.0326), suggesting a better ovarian response in the control group. Due to a strong patient preference, nearly exclusively against ultra-long downregulation (even though patients were thoroughly informed of the potential benefits), the targeted sample size could not be achieved and the trial was stopped prematurely. Conditional power analysis revealed that the probability of confirming the study hypothesis if the study were completed would be low. We hypothesize that in patients with prior complete surgical treatment of endometriosis, the ultra-long protocol does not enhance ART-CPHB rates. Patient's concerns and preferences regarding possible side-effects, and delay of ART treatment start with the ultra-long protocol should be taken into account when considering this type of treatment in women with endometriosis. C.T. was during 2 years funded by a grant from the Clinical research Foundation of UZ Leuven (KOF) and during 2 years by the Research Foundation-Flanders (FWO grant number: 1700816N). C.T. reports grants from Clinical Research Foundation of the University Hospitals of Leuven (KOF), grants from Fund for Scientific Research Flanders (FWO), during the conduct of the study; grants, non-financial support and other from Merck SA, non-financial support and other from Gedeon Richter, non-financial support from Ferring Pharmaceuticals, outside the submitted work. T.D. is vice president and head of Global Medical Affairs Fertility, Research and Development, Merck KGaA, Darmstadt, Germany. He is also a professor in Reproductive Medicine and Biology at the Department of Development and Regeneration, Group Biomedical Sciences, KU Leuven (University of Leuven), Belgium and an adjunct professor at the Department of Obstetrics and Gynecology in the University of Yale, New Haven, USA. Neither his corporate role nor his academic roles represent a conflict of interest with respect to the work done by him for this study. A.C. reports personal fees from Merck S.p.A., outside the submitted work. The other co-authors have no conflict of interest. UZ Leuven trial registry SS55300, EudraCT number 2013-000993-32, clinicaltrials.gov NCT02400801. Registration for EudraCT on 1 March 2013. 4 September 2013.