The purpose of the current study is to identify the level of psychological flow and its relationship to the professional ambition of students of scientific colleges who are expected to graduate from public Jordanian universities. The study sample consisted of (315) male and female students expected to graduate. Two scales were developed: Psychological Flow Scale, and professional ambition, and their psychometric characteristics were verified. The results of the study concluded that there was an average level of psychological flow and professional ambition among students in scientific faculties. It was also found that there was a positive correlational relationship between psychological flow and professional ambition, and they do not differ according to gender. It is necessary to recommend the development of skills and positive phenomena for students.