Food Products Research Articles

Integrated metabolomic and transcriptomic strategies to reveal adaptive mechanisms in barley plant during germination stage under waterlogging stress.

Barley (Hordeum vulgare L.) is an important cereal crop used in animal feed, beer brewing, and food production. Waterlogging stress is one of the prominent abiotic stresses that has a significant impact on the yield and quality of barley. Seed germination plays a critical role in the establishment of seedlings and is significantly impacted by the presence of waterlogging stress. However, there is a limited understanding of the regulatory mechanisms of gene expression and metabolic processes in barley during the germination stage under waterlogging stress. This study aimed to investigate the metabolome and transcriptome responses in germinating barley seeds under waterlogging stress. The findings of the study revealed that waterlogging stress sharply decreased seed germination rate and seedling growth. The tolerant genotype (LLZDM) exhibited higher levels of antioxidase activities and lower malondialdehyde (MDA) content in comparison to the sensitive genotype (NN). In addition, waterlogging induced 86 and 85 differentially expressed metabolites (DEMs) in LLZDM and NN, respectively. Concurrently, transcriptome analysis identified 1776 and 839 differentially expressed genes (DEGs) in LLZDM and NN, respectively. Notably, the expression of genes associated with redox reactions, hormone regulation, and other biological processes were altered in response to waterlogging stress. Furthermore, the integrated transcriptomic and metabolomic analyses revealed that the DEGs and DEMsimplicated in mitigating waterlogging stress primarily pertained to the regulation of pyruvate metabolism and flavonoid biosynthesis. Moreover, waterlogging might promote flavonoid biosynthesis by regulating 15 flavonoid-related genes and 10 metabolites. The present research provides deeper insights into the overall understanding of waterlogging-tolerant mechanisms in barley during the germination process.

Current State of Research on Health-Promoting Functional Properties in Berry-Based Foods.

This review aims to consolidate recent findings on the development and functional validation of berry-based foods while proposing guidelines for future advancements. Current investigations on berry-based functional foods (dairy and bakery products, snacks, etc.) emphasize their potential health benefits, including antioxidant effects, glycemic control, enzyme modulation, among others. Although there is valuable information on the capacity of berry-derived food products to confer health benefits, only 10% of the reviewed publications reached the final validation stage of the formulated product through in vivo assays. The analyzed publications were classified according to the approach used to study the functional potential of the developed berry-based products, considering simple spectrophotometric analysis, in vitro biological studies, and in vivo studies. Guidelines for a successful development of berry-based health enhancing foods were presented. Future research should include functional validation of final food products and confirm their bioactivity through in vivo studies.

Preparation of CHS-Fe3O4@@ZIF-8 peroxidase-mimic with an ultra-thin hollow layer for ultrasensitive electrochemical detection of kanamycin.

Ahighly sensitive and selective electrochemical biosensor was developedfor the detection of kanamycin using a core-hollow-shell structured peroxidase-mimic nanozyme, CHS-Fe₃O₄@@ZIF-8. The synthesized CHS-Fe3O4@@ZIF-8 was characterized with scanning electron microscopy, transmission electron microscopy, andX-ray photoelectron spectroscopy. Itwasfound that the CHS-Fe3O4@@ZIF-8 exhibits excellent peroxidase-like activity due to its ultra-thin hollow layer. Besides, CHS-Fe3O4@@ZIF-8 functionalized with complementary chains of kanamycin aptamer was anchored to the electrode surface via complementary base pairing with the kanamycin aptamer. Upon the presence of kanamycin, a strand displacement reaction was triggered leading to a reduction in the number of the CHS-Fe3O4@@ZIF-8, which slowed down the catalytic reaction of the substrate 3,3',5,5' -tetramethylbenzidine (TMB) facilitated by CHS-Fe3O4@@ZIF 8. Differential pulse voltammetry (DPV) was employed to measure and record changes in peak current resulting from catalytic oxidation product formation (oxidation product of TMB). The electrochemical signal exhibited a linear relationship with logarithmic variations in kanamycin concentration within a range spanning from 10 to 8000pM and achieved an impressive detection limit as low as 7.52pM. Furthermore, successful detection of kanamycin content in serum samples using this sensor demonstrated its good specificity and reproducibility. These findings indicate that theconstructed electrochemical kanamycin sensor holds significant potential for practical applications. The biosensor demonstrated high selectivity, distinguishing kanamycin from other antibiotics, and exhibited good reproducibility, making it reliable for practical applications. The successful detection of kanamycin in serum samples further underscores the sensor's potential for real-world applications, particularly in monitoring antibiotic residues in food products and clinical diagnostics.

A Comprehensive Review of Phenolic Compounds in Chia Seeds and Their Applications in the Food Industry.

Chia seeds (Salvia hispanica L.) have emerged as a significant focus in the food industry due to their rich nutritional profile and health-promoting attributes. They are a major powerhouse of bioactive compounds such as flavonoids, phenolic acids, and tocopherols that have been shown to possess anti-inflammatory, anti-diabetic, anti-cholesterol functions, enhance cognitive performance, and improveheart health. This article provides an in-depth review of the phenolic compounds in chia seeds and various fractions such as oil, and chia meal, their bioaccessibility, along with unique applications in food products. Additionally, 'green techniques' for extracting chia oil, as a sustainable alternative to conventional methods, have also been discussed. The findings presented in this review suggest that chia seeds, due to their bioactive components and versatile functional properties, are well-positioned to be a valuable ingredient in the development of novel foods, contributing to better health outcomes and innovation in food processing.

Analysis of agricultural land condition in Western Kazakhstan from 1991 to 2023: the impact of climate change.

Kazakhstan's insufficient food production contributes to its dependency on food imports, highlighting the need for science-based technologies to address land degradation and boost domestic production. The privatisation of land and the establishment of a market economy led to the division of collective farms and significant land fragmentation, resulting in a reduction of agricultural land by 10.6 million ha in the West Kazakhstan region, particularly between 1991 and 2000. Desertification and soil degradation have led to decreased soil fertility, adversely affecting the agricultural industry. Over the last 30years, the area of eroded soils has increased by 5-9%. As of 2022, over 16.7% of agricultural land remains unused, a substantial rise from 1991. This study aims to investigate the qualitative and quantitative transformations of agricultural land in the region over the past three decades and to assess the impact of climate change on land degradation processes. To achieve this, cartographic analysis of NDVI3g (Global Inventory Monitoring and Modelling System [GIMMS]) data for 1990-2022 was conducted, employing linear ordinary least squares and median Theil-Sen trend methods to identify long-term vegetation trends. The results showed a negative trend in agricultural lands with a decline rate of 0.0025 per year (P = 0.009). However, in the past 13years, a positive trend was observed in only three regions, with an average increase of 0.007 per year (P = 0.03). These findings are statistically significant and highlight the growing impact of climatic factors on agricultural and natural ecosystems.

Vancomycin-resistant Enterococcus prevalence and its association along the food chain: a systematic review and meta-analysis.

Vancomycin-resistant Enterococcus (VRE) are present across the One Health continuum and pose a considerable risk for transmission along the food chain. This systematic review and meta-analysis estimates the prevalence of VRE colonization in livestock, food of animal origin, and in human populations. Embase, MEDLINE and CAB Abstracts were searched for eligible literature. A total of 54 manuscripts passed inclusion criteria by providing prevalence estimates of VRE in a human population and at least one of either livestock or food. Random effects meta-analysis was conducted to determine prevalence estimates, and risk of bias in pooled estimates was assessed using funnel plots and Egger regression. Global pooled prevalence of VRE colonization was highest in poultry and poultry meat at 16% (95% CI: 6%-28%) and 15% (95% CI: 1%-39%), respectively. Human-associated VRE colonization was highest in livestock workers, with a pooled prevalence of 11% (95% CI: 2%-25%), and lowest in the general public at 2% (95% CI: 0%-3%). Meta-regression demonstrated that human VRE prevalence increased at a rate of 0.75% (95% CI: 0.46%-1.04%; P < 0.001) per 1% increase in livestock VRE colonization. This meta-analysis established a clear link of VRE across One Health sectors. VRE colonization is likely elevated for those in contact with colonized animals or contaminated food products. Quality of evidence in pooled prevalence estimates was limited by publication bias and heterogeneity. The results of this study enhance calls for a One Health approach for mitigating the global burden of priority antimicrobial resistance pathogens.

Bibliometric Analysis of Probiotic Bacillus in Food Science: Evolution of Research Trends and Systematic Evaluation.

With the in-depth and comprehensive research on probiotic Bacillus, it has become a hot topic in food science. However, the current status of research using bibliometric analysis to assess the application of probiotic Bacillus in food science has not been comprehensively reviewed. The Web of Science (WOS) database was used in this review's bibliometric analysis to determine the hotspots for research as well as the extent of completed experiments. Furthermore, a systematic review was conducted on the research hotspots of probiotic Bacillus in food science. The comprehensive analysis showed it was a growing and global research field. The keywords with high frequency mainly included "spore," "strain," and "production," which were research hot topics in the last decade. The application of the spore form or nutrient cells of probiotic Bacillus in industrialized food production through nutrient fortification, fermentation agents, and highly efficient synthesis of metabolites showed great development potential.

Certified reference materials for GMO analysis-more than 25 years of GMO CRM production at EC JRC.

Certified reference materials (CRMs) play a crucial role in ensuring the quality of analytical measurements. Particularly, the EU labelling legislation on genetically modified organisms (GMOs) in food and feed products explicitly requires CRMs for its implementation. The CRMs are used to calibrate and control the quantitative real-time polymerase chain reaction (qPCR) method and support official control laboratories, such as National Reference Laboratories (NRLs), in maintaining their ISO/IEC 17025 accreditation. The Joint Research Centre of the European Commission (EC JRC) is specialized in the production of reference materials and has been producing GMO CRMs since 1998. Together with a validated event-specific qPCR method, these GMO CRMs form the EU reference system for the quantification of EU-authorized GMO events in food and feed products and ensure a common GMO labelling threshold. This article gives a retrospective view on the more than 25 years of GMO CRM production at JRC. It describes requirements for GMO CRMs in view of an EU market authorization under (EC) No. 1829/2003. The evolution and major improvements of the production of GMO CRMs at JRC are summarized as well as the current understanding of theEU's GMO reference system for GMO quantification and its impact on commutability. It provides insights into GMO CRM sales and their worldwide distribution. This information may be useful for policymakers and researchers in understanding the current EU GMO measurement landscape and to anticipate possible future demands related to GMO events based on new genomic techniques (NGTs).

The Impact of Rural Alimentation on the Motivation and Retention of Indigenous Community Health Workers in India: A Qualitative Study.

Rural health care delivery remains a global challenge and India is no exception, particularly in regions with Indigenous populations such as the state of Jharkhand. The Community Health Centres in Jharkhand, India, are staffed by Indigenous workers who play a crucial role in bridging the health care gap. However, their motivation and retention in these challenging areas are often influenced by a complex mix of sociocultural and environmental factors. One such significant but understudied influencing factor is alimentation, or nutrition, in rural settings. Previous studies have identified several motivators, including community ties, cultural alignment, job satisfaction, and financial incentives. However, the role of alimentation in their motivation and retention in rural areas has not been sufficiently explored. This study aims to explore how the strong bond with locally produced food products impacts the retention of Indigenous community health workers (CHWs) in Jharkhand, India, and shed light on a crucial aspect of rural health care workforce sustainability. This study adopted a phenomenological research design to explore the lived experiences and perspectives of Indigenous CHWs in Jharkhand. A purposive sampling method was used to select CHWs who had worked in rural areas for at least five years. Data were collected through semistructured interviews, focusing on the participants' experiences of rural alimentation and how it influences their motivation and retention for rural health care. The interviews were audio recorded, transcribed, and analyzed using thematic analysis to identify common themes and patterns in their experiences related to nutrition and retention. The study revealed that rural alimentation plays a significant role in both the motivation and retention of CHWs in Jharkhand. CHWs who experienced consistent access to local food reported higher job satisfaction, better physical well-being, and a stronger commitment to their roles. It has also been perceived that consuming nutrient-dense food products decreases the risk of chronic illness among rural populations. Additionally, community support systems related to alimentation were found to be crucial in maintaining motivation, with many CHWs emphasizing the importance of local food availability and cultural ties. The findings suggest that improving access to organic nutrition can positively influence the retention of CHWs in rural areas. Indigenous communities have unique food habits and preferences deeply rooted in agriculture and arboriculture. Their traditional eating practices are integral to their rich cultural heritage, with significant social, symbolic, and spiritual importance. This study highlights the critical role of rural alimentation in motivating and retaining CHWs in rural Community Health Centres in Jharkhand. Therefore, addressing organic versus conventional food in rural health care policies plays a vital role in improving the retention rates of CHWs. By recognizing the interconnectedness of nutrition and workforce sustainability, health care systems can better support Indigenous CHWs and continue delivering health care services.

Point-of-need diagnostics in a post-Covid world: an opportunity for paper-based microfluidics to serve during syndemics.

Zoonotic outbreaks present with unpredictable threats to human health, food production, biodiversity, national security and disrupt the global economy. The COVID-19 pandemic-caused by zoonotic coronavirus, SARS-CoV2- is the most recent upsurge of an increasing trend in outbreaks for the past 100 years. This year, emergence of avian influenza (H5N1) is a stark reminder of the need for national and international pandemic preparedness. Tools for threat reduction include consistent practices in reporting pandemics, and widespread availability of accurate detection technologies. Wars and extreme climate events redouble the need for fast, adaptable and affordable diagnostics at the point of need. During the recent pandemic, rapid home tests for SARS-CoV-2 proved to be a viable functional model that leverages simplicity. In this perspective, we introduce the concept of syndemnicity in the context of infectious diseases and point-of-need healthcare diagnostics. We also provide a brief state-of-the-art for paper-based microfluidics. We illustrate our arguments with a case study for detecting brucellosis in cows. Finally, we conclude with lessons learned, challenges and opportunities for paper-based microfluidics to serve point-of-need healthcare diagnostics during syndemics.

Unveiling the Potential of Southern Elderberry (Sambucus australis): Characterization of Physicochemical Properties, Carbohydrates, Organic Acids and Biophenols.

Sambucus australis is a wild species with purple fruits like berries. It is native from South America and can be found in Argentina, Uruguay, Paraguay, Bolivia, and Brazil. A comprehensive characterization of S. australis fruiting period and ripening could provide valuable information on the stage of development suitable for formulating typical and new food and cosmetic products, as well as it could contribute to reveals its nutritional value. This study aimed to examine the evolution of fruit size and weight alongside the accumulation patterns of sugars, organic acids, biophenols and antioxidant activity during different development stages of S. australis fruit, assessing its potential as a source of health-promoting compounds. The increase in total sugar (269.59g/kg dry weight), together with the decrease in the total organic acids (321.63g/kg dry weight) at the fully ripe stage, indicates that the fruit is sweet. This suggests that it is an appealing product to be consumed when it is fully ripe. The fatty acid composition contains significant levels of α-linolenic, linoleic, oleic and palmitic acids. It is characterized by high ratios of polyunsaturated fatty acids to saturated fatty acids, ranging from 5.8 to 6.4. S. australis fruits appeared to possess good levels of biophenols at fully ripe fruits (4709.7µg/g dry weight) together with antioxidant activity (higher than 80%), so, it could be considered as a functional food.

Mucilage-based composites films and coatings for food packaging application: A review.

Developing sustainable and eco-friendly packaging solutions has garnered significant interest in recent years. Mucilage-based coatings and composites offer a promising approach due to their biodegradability, renewable nature, and ability to enhance food quality protection. This review paper discusses the impact of mucilage-based composites and coatings on various packaging applications, focusing on their physical, mechanical, morphological, barrier, and functional properties. These materials' adaptability, flexibility, transparency, and compatibility with various food products make them highly suitable for food packaging. The morphological structure of mucilage-based films contributes to improved adhesion, surface roughness, and homogeneity. Enhanced barriers against moisture, oxygen, and other gases extend the shelf life of packaged food while maintaining its quality. Mucilage from different plant sources exhibits functional properties such as antioxidant and antimicrobial activities, which enhance food preservation. These attributes and mucilage's biocompatibility and biodegradability align with the growing demand for environmentally friendly packaging options. The review also addresses cost-effectiveness, regulatory compliance, consumer acceptance, recycling infrastructure compatibility, supply chain considerations, and the need for ongoing innovation. Future advancements in mucilage-based packaging will depend on optimizing performance, scalability, and sustainability. By understanding the effects on physio-mechanical, morphological, barrier, and functional attributes, mucilage-based composites and coatings hold great potential for advancing sustainable food packaging solutions.

An integrated multiphase dynamic genome-scale model explains batch fermentations led by species of the Saccharomyces genus.

During batch fermentation, a variety of compounds are synthesized, as microorganisms undergo distinct growth phases: lag, exponential, growth-no-growth transition, stationary, and decay. A detailed understanding of the metabolic pathways involved in these phases is crucial for optimizing the production of target compounds. Dynamic flux balance analysis (dFBA) offers insight into the dynamics of metabolic pathways. However, explaining secondary metabolism remains a challenge. A multiphase and multi-objective dFBA scheme (MPMO model) has been proposed for this purpose. However, its formulation is discontinuous, changing from phase to phase; its accuracy in predicting intracellular fluxes is hampered by the lack of a mechanistic link between phases; and its simulation requires considerable computational effort. To address these limitations, we combine a novel model with a genome-scale model to predict the distribution of intracellular fluxes throughout batch fermentation. This integrated multiphase continuous model (IMC) has a unique formulation over time, and it incorporates empirical regulatory descriptions to automatically identify phase transitions and incorporates the hypotheses that yeasts might vary their cellular objective over time to adapt to the changing environment. We validated the predictive capacity of the IMC model by comparing its predictions with intracellular metabolomics data for Saccharomyces uvarum during batch fermentation. The model aligns well with the data, confirming its predictive capabilities. Notably, the IMC model accurately predicts trehalose accumulation, which was enforced in the MPMO model. We further demonstrate the generalizability of the IMC model, explaining the dynamics of primary and secondary metabolism of three Saccharomyces species. The model provides biological insights consistent with the literature and metabolomics data, establishing it as a valuable tool for exploring the dynamics of novel fermentation processes.IMPORTANCEThis work presents an integrated multiphase continuous dynamic genome-scale model (IMC model) for batch fermentation, a crucial process widely used in industry to produce biofuels, enzymes, pharmaceuticals, and food products or ingredients. The IMC model integrates a continuous kinetic model with a genome-scale model to address the critical limitations of existing dynamic flux balance analysis schemes, such as the difficulty of explaining secondary metabolism, the lack of mechanistic links between growth phases, or the high computational demands. The model also introduces the hypothesis that cells adapt the FBA objective over time. The IMC improves the accuracy of intracellular flux predictions and simplifies the implementation process with a unique dFBA formulation over time. Its ability to predict both primary and secondary metabolism dynamics in different Saccharomyces species underscores its versatility and robustness. Furthermore, its alignment with empirical metabolomics data validates its predictive power, offering valuable insights into metabolic processes during batch fermentation. These advances pave the way for optimizing fermentation processes, potentially leading to more efficient production of target compounds and novel biotechnological applications.

Accumulation of heavy metals in food products from territories adjacent to the Alaverdi copper‐chemical combine, Armenia

Accumulation of heavy metals in food products from territories adjacent to the Alaverdi copper‐chemical combine, Armenia

Exploring Nexus between food security and the loss of agro-biodiversity: a study in Madhya Nepal, Lamjung

Nepal has made significant strides in enhancing food security, achieving a moderate hunger index of 15.0 in 2023. However, the relationship between food security and reduced agro-biodiversity remains underexplored. This study investigates this interplay in Madhya Nepal Municipality, Lamjung District, Gandaki Province. Using Food Consumption Score (FCS) and Reduced Coping Strategies Index (rCSI), the study assesses food availability, nutrition sources, and coping mechanisms. The methodology includes field surveys, interviews, and analysis of 30 years of climate data. Ward-wise investigations reveal challenges and opportunities through quantitative and qualitative approaches. Motivated by the complex dynamics affecting food security and agricultural practices amid evolving environmental conditions, this research highlights community struggles and opportunities. It emphasizes the need for a balance between traditional and hybrid agriculture, calling for adaptive strategies to address climate change impacts. Integrating traditional knowledge with scientific innovations is crucial for sustainable food production. Results show higher food stock in some wards due to drought-resistant crops, while others face challenges with traditional farming reliance. Climate data analysis indicates significant temperature increases, affecting rice adaptation and traditional practices. Increased adoption of hybrid crops, supported by irrigation and improved technical knowledge, boosts cereal productivity. The study concludes a positive correlation between agro-biodiversity and food security, highlighting diverse agricultural systems' importance in mitigating climate change challenges.

Ecotoxicological impact of succinate dehydrogenase inhibitor (SDHI) fungicides on non-targeted organisms: a review.

As the global population continues to grow, the use of pesticides to increase food production is projected to escalate. Pesticides are critical in plant protection, offering a powerful defense against fungal diseases such as apple scab, leaf spot, sclerotinia rot, damping off, sheath blight, and root rot, which threaten crops like cereals, corn, cotton, soybean, sugarcane, tuberous vegetables, and ornamentals. Succinate Dehydrogenase Inhibitor (SDHI) fungicides represent a novel class essential for controlling fungal pathogens and bolstering food security. However, the impact of SDHIs on non-target organisms, including freshwater and terrestrial invertebrates, crustaceans, and oligochaetes, remains insufficiently understood. Empirical studies indicate that SDHIs can induce mortality, mitochondrial dysfunction, oxidative stress, and developmental delays in non-target organims. Additionally, the environmental persistence of these compounds raises concerns about their potential for ecological disruption. The effects of SDHIs on pollinating species and the possible transgenerational transmission of harmful effects warrant further investigation. Comprehensive transcriptomic analyses are necessary to elucidate the molecular disturbances and adverse outcome pathways triggered by SDHIs. Furthermore, there are emerging concerns about the endocrine-disrupting potential of SDHIs in aquatic organisms. For the first time, this review aims to synthesize existing knowledge on the ecotoxicological impacts of SDHIs on non-target organisms and identify critical research directions to address the ecological challenges posed by their use.

A Novel Solar Rooftop Agriculture System Integrated with CNT Nanofluid Spectral Splitter for Efficient Food Production

A Novel Solar Rooftop Agriculture System Integrated with CNT Nanofluid Spectral Splitter for Efficient Food Production

New opportunities for biologically and chemically mediated adsorption and precipitation of phosphorus from wastewater.

Biologically mediated adsorption and precipitation of phosphorus (P) from waste streams can restrict environmental P discharges. Here, we appraise progress in this field over the past decade. The research discipline has grown considerably in recent years. Industry 'wastes', including steel slags, continue to show promise as adsorbents with exceptionally high P retention capacities (>500mgPg-1). Hydrotalcite, a nanomineral, offers prospects as a P removal technology with imbedded climate change mitigation capacity. Biomineral struvite formation, driven by microbial processes, offers an exciting P removal and recovery approach that can be applied to diverse wastewater types due to its feedstock-independent mechanisms, emerging immobilisation techniques and adaptability to mixed cultures. All of these factors facilitate efficient nutrient recycling and scalable application to the wastewater industry. Adsorbed and precipitated P can be applied to cropland to offset dependence on conventional fertiliser inputs. Therefore, in addition to water treatment, these biologically mediated processes also offer opportunities to support food production. Moreover, as many of the input materials covered in this review are industry byproducts and common organic materials, the removal of P from waste streams by adsorption and precipitation offers strong circularity potential that aligns with the UN's Sustainable Development Goals. We call for future work to focus on long-term full-scale trials involving community, government and industry partners.

Computational simulation of graphene/h-BN nanopores for single-molecule herbicide sensing.

The growing world population and climate change are key drivers for the increasing pursuit of more efficient and environmentally-safe food production. In this scenario, the large scale use of herbicides demands the development new technologies to control&#xD;and monitor the application of these compounds, due to their several environmental and health-related problems. Motivated by all these issues, in this work, a hybrid graphene/boron nitride nanopore is explore to detect/identify herbicide molecules (Glyphosate, AMPA, Diuron, and 2,4-D). Solid-state nanopores based on 2D materials have been widely explored as novel generation sensors capable of single-molecule resolution. The present investigation combines the density functional theory (DFT) and non-equilibrium Green's function (NEGF) method to assess the interaction of each herbicide with the nanopore and how its interaction modulates the device's electronic&#xD;transport properties. The device's sensitivity spreads from 9.0 up to 27.0% when probed at different gate voltage values. Overall, the proposed device seems to be sensitive and selective to be considered as a promising single-molecule herbicide sensor.

Unraveling the nutritional potential of millet by-products through extraction of high value compounds for the development of novel food products.

Millets are drought-resistant crops that generate significant amount of by-products (bran, husk, stalk etc.) during harvesting and processing. These by-products are storehouse of nutrients and high value compounds including polyphenols, dietary fiber, proteins etc. However, these by-products remain underutilized and generally discarded, burned or used as feedstock causing adverse impact on the environment and human health in addition to loss of valuable nutrients. Therefore, the valorization of millet by-products offers sustainable approach to enhance food product innovation while reducing agricultural waste. Green extraction techniques can be employed to recover antioxidants, phenolics, and bioactive peptides from these by-products. The incorporation of these ingredients into food products can significantly improve the nutritional profile, functional characteristics, like antioxidant, prebiotic, anti-diabetic, and anticarcinogenic properties. The review highlights the feasibility of upcycling millet by-products into high-value components, which can address the growing demand for health-oriented food products contributing towards food security, sustainability and circular economy.