This study explores the impact of Indoor Environment Quality (IEQ) on the health and productivity of office workers in an office building in Fujisawa, Kanagawa, Japan. Previous studies have shown that IEQ can affect the physiological responses of occupants, such as of skin temperature, heart rate, and metabolic rate, which are indicators of health and productivity. However, most studies took place in controlled laboratory environments, which may not accurately represent real-life experiences. The study collected subjective and objective data from actual occupied office space, including on perceptions of IEQ, health, and productivity, and measurements of IEQ parameters such as on the thermal environment, light environment, indoor air quality, and acoustics. The study used correlation and linear regression methods to examine the relationship between IEQ, physiological data, and subjective responses to health and productivity. The stable thermal environment and low physical intensity of office work may contribute to the weak correlation between physiological data, thermal responses, and health–productivity variables. The results of this study can provide insights into how IEQ affects the psychological responses, well-being, and performance of office workers in real-world settings.