Chicken Production Research Articles

Evaluating Farmers' Awareness and Adoption of Housefly Maggots as Alternative Protein for Broiler Chicken Production in Mbarara District, Western Uganda

Evaluating Farmers' Awareness and Adoption of Housefly Maggots as Alternative Protein for Broiler Chicken Production in Mbarara District, Western Uganda

Quantitative prediction of minced chicken gel strength under ultrasonic treatment by NIR spectroscopy coupled with nonlinear chemometric tools evaluated using APaRPs

Achieving the ideal gel strength is essential for desired texture in minced chicken products. This study developed a rapid, non-destructive method using near-infrared (NIR) spectroscopy and nonlinear chemometric modeling to predict minced chicken gel strength under ultrasonic treatment. Initially, minced chicken samples were subjected to high-intensity ultrasound for 0–50 min. This was followed by heat-induced gelation. Gel strength was conventionally measured, and NIR spectra were collected. Nonlinearity between gel strength and spectral data was confirmed using augmented partial residual plots (APaRPs). Subsequently, nonlinear support vector machine (SVM) and extreme learning machine (ELM) models were developed using full NIR spectra and variable selection methods, including uninformative variable elimination (UVE), competitive adaptive reweighting (CARS), and genetic algorithms (GA). GA proved most effective for enhancing model performance, achieving the highest predicted coefficient of determination (Rp2 = 0.8772) with the ELM model, demonstrating potential for rapid, non-destructive prediction of minced chicken gel strength quality.

Empowerment of Chicken Farmers Based on Cleaner Production and Strengthening of Product Added Value through Entrepreneurial Knowledge Enhancement

Given the environmental and health challenges, chicken poultry farms are now facing an increasing urgency to implement cleaner production. The application of the Cleaner Production concept is expected to be a solution to increase business efficiency and sustainability. One way is to process chicken poultry farm waste into valuable products, such as organic fertilizers, thereby reducing environmental negative impacts. This research method uses a Research and Development (R&D) approach with a three-box method. The community participates in a training program focusing on the innovation of processed chicken products, namely the manufacture of dimsum. This training covers theory and practices about Cleaner Production and dimsum-making techniques. The method used in building a complete solution in community service was obtained by Borg and Gall with the calculation of a questionnaire used to measure the achievement of induction of knowledge and skills based on the three-box method. The implementation of community service programs has shown an increase in the skills of partner Small and Medium Enterprises (SMEs) by 89%, an increase in knowledge by 97.6%, and the development of ideas for sustainability by 98.8%. This community service provides a complete solution to increase cleaner production, effectively improving skills and adding value to UD Sumber Mandiri processed chicken products.

Influence of Corona Virus Disease (COVID-19) on Consumption and Purchasing Patterns of Poultry Products from Three Selected Districts in Sri Lanka

The study reported herein investigated the effects of COVID-19 pandemic on purchasing and consumption patterns of poultry products among the communities in Kandy, Gampaha and Galle districts of Sri Lanka. A pre-tested questionnaire was used to collect data from 629 respondents. The questionnaire explored different socio-economic and health factors affecting on consumption and purchasing frequencies of chicken meat, meat products and eggs before and during the pandemic. The results revealed that the pandemic has made an impact on consumption frequencies of chicken or processed chicken products. Weekly purchasing amount of chicken or processed chicken products has been declined during the pandemic. Many respondents increased their egg consumption during the pandemic. Stocking poultry products during the pandemic has also increased. The study concluded that though the purchasing frequencies and the amount of chicken or processed chicken products have been changed consequently to the pandemic, neither the purchasing method nor purchasing brand has been changed. The pandemic had no influence on buying patterns for eggs but has contributed to increased egg consumption frequency among the public.

Baby Foods: 9 Out of 62 Exceed the Reference Limits for Acrylamide.

Acrylamide (AA) is a contaminant resulting from the Maillard reaction and classified by the International Agency for Research on Cancer (IARC) as a probable carcinogen in Group 2A, with proven neurotoxic effects on humans. European Union (EU) Regulation No. 2017/2158 is currently in force, which establishes measures meant to reduce AA levels in food and sets reference values, but not legal limits, equal to 40 and 150 μg/kg AA in processed cereal-based foods intended for infants and young children and in biscuits and rusks, respectively. For this reason, sixty-two baby foods were analyzed using ultra-high performance liquid chromatography with diode array detector and quadrupole time-of-flight mass spectrometry (UHPLC-DAD-Q-TOF/MS) to check whether industries were complying with these values, even though AA control is not legally mandatory. In total, 14.5% of the samples exceeded the reference values; these were homogenized chicken products (211.84 ± 16.53, 154.32 ± 12.71, 194.88 ± 7.40 μg/kg), three biscuits (276.36 ± 0.03, 242.06 ± 0.78, 234.78 ± 4.53 μg/kg), a wheat semolina (46.07 ± 0.23 μg/kg), a homogenized product with plaice and potatoes (45.52 ± 0.28 μg/kg), and a children's snack with milk and cocoa (40.95 ± 0.32 μg/kg). Subsequently, the daily intake of AA was estimated, considering the worst-case scenario, as provided by the consumption of homogenized chicken products and biscuits. The results are associated with margins of exposure (MOEs) that are not concerning for neurotoxic effects but are alarming for the probable carcinogenic effects of AA.

Integrative analysis of RNA-seq and Ribo-seq reveals that lncRNA-GRN regulates chicken follicular atresia through miR-103-3p/FBXW7 axis and encoding peptide

Follicular atresia in chickens seriously reduced the egg production and economic benefits of chickens. LncRNA plays a key role in the process of follicular atresia. In this study, RNA-seq and Ribo-seq were performed on normal and atretic follicles of Dahen broilers to screen out lncRNAs that may regulate follicle atresia, and to study the molecular mechanisms of their regulation. GRN granulin precursor (lncGRN, ID: 101748909) was highly expressed in atretic follicles with translational ability. A molecular regulatory network of lncGRN/miR-103-3p/FBXW7 was constructed through bioinformatics analysis and dual luciferase reporting. LncGRN promoted the expression of FBXW7 by adsorption of miR-103-3p, thereby inhibiting the proliferation of chicken granulosa cells (GCs), promoting apoptosis of chicken GCs and inhibiting steroid hormone synthesis thus induced follicular atresia. Meanwhile, we also found a micropeptide named GRN-122aa derived by lncGRN which can promote follicular atresia. In conclusion, our study found that lncGRN promoted follicular atresia through the lncGRN/miR-103-3p/FBXW7 axis and the translation micropeptide GRN-122aa. This study provided new insight into the post-transcriptional regulation mechanism of lncGRN suggesting that lncGRN may act as a potential to regulate chicken follicle development, and provided a theoretical argument for further improving the egg production of chickens through molecular breeding.

Enhancing Laying Hens' Performance, Egg Quality, Shelf Life during Storage, and Blood Biochemistry with Spirulina platensis Supplementation.

Enhancing the sustainability of chicken farming involves improving health and productivity and product qualities. This study explores the influence of Spirulina platensis (SP) supplementation on the productivity, egg quality, shelf life during storage, and blood biochemistry of laying hens. A total of 192 thirty-nine-week-old White Leghorn hens were randomly divided into 4 dietary groups: a control group and 3 treatment groups receiving 2.5 g/kg, 5 g/kg, or 10 g/kg of SP, respectively. The study was conducted for six weeks with measuring feed intake, feed conversion ratio, egg production, egg quality, shelf life, and blood biochemistry. The results demonstrated significant enhancements in egg weight (p < 0.05) and egg mass (p < 0.05) in the treatment of SP groups. The SP treated hens showed significant improvements in yolk color (p < 0.05) and Haugh unit scores (p < 0.05). The SP supplementation showed a hepatoprotective effect, as indicated by significant reduction in Alanine aminotransferase (ALT) (p < 0.05) and alkaline phosphatase (ALP) (p < 0.05) levels; however, increases in total protein, albumin, and globulin levels were observed. Furthermore, the egg quality of stored eggs for 21 days linearly increased with increments in the SP levels. In conclusion, it can be speculated that adding SP at 2.5 g/kg and 5 g/kg can significantly improve the productivity of laying hens, eggs' quality, shelf life, and blood biochemistry, thereby contributing to a more sustainable and efficient chicken production.

Utilizing foam-structured hydroxypropyl methylcellulose oleogels with curcumin for deep-fried chicken nuggets in carnauba wax-canola oil oleogel

In this study, foam-structured hydroxypropyl methylcellulose was used to structure cod liver oil into oleogel. Different concentrations of curcumin (0, 1.1, 2.2, 3.3, and 4.4 mg/100g of chicken nuggets) were added to the oleogels and incorporated into the chicken nuggets. Subsequently, samples were deep-fried in an oleogel frying medium prepared with carnauba wax and canola oil (10g/100g). Our results revealed that the addition of 4.4 mg/100g of curcumin led to the highest fat uptake reduction and moisture retention compared to the control and 1.1 mg/100g of curcumin (P < 0.05). Additionally, the addition of 4.4 mg/100g of curcumin exhibited consistently higher oxidative stability, as indicated by thiobarbituric acid reactive substances (TBARS), during the 30-day storage period at −18 °C compared to the control (P < 0.05). Notably, treated samples did not show any alteration in ash and protein contents, color, texture, pH values, sensory attributes, or microscopic structures of the fried chicken products. Furthermore, fatty acid analysis revealed that the samples containing 4.4 mg/100g of curcumin had significantly lower levels of saturated fatty acids and higher levels of monounsaturated and polyunsaturated fatty acids than the control (P < 0.05). Overall, the study demonstrates that adding curcumin in a carrier setting to deep-fried chicken products can improve the products by reducing fat uptake and extending oxidative stability.

Consumption patterns of fresh and frozen chicken: A Ghanaian food policy perspective

AbstractDespite the global increase in poultry consumption, there are different preferences for chicken products in different countries. In Ghana, two main categories dominate the market: live/fresh whole chickens and imported frozen chicken parts. This study leverages data from 13,201 households to explore Ghanaian demand dynamics for these products using the Quadratic Almost Ideal Demand System. Findings reveal stark differences between the two. Live/fresh whole chickens, considered luxury goods, display a complex interplay with other non‐chicken meat products, cultural festivities, and various sociodemographic factors. Meanwhile, frozen chicken parts, seen as necessities, exhibit different demand shifters and peak consumption periods. Importantly, these two categories emerge as distinct products, signaling to policymakers the need for differentiated strategies. Simulated policy scenarios further emphasize that reducing prices of live/fresh chickens significantly boosts domestic chicken demand more than policies raising prices of frozen parts. However, the broader implications on alternative protein sources differ between the two policy types. We urge policymakers to adopt an integrated approach, considering these nuances, to strengthen Ghana's domestic poultry sector without unintended market disruptions. [EconLit citations: Q11, Q13, Q17, Q18, D12].

Assessment of Nutritional Quality of Plant-based Meat Alternatives Sold in the Philippines

Plant-based meat alternatives (PBMAs) have been included in Filipino’s diet since their availability in the Philippines, making it important to evaluate their nutritional profile to assist consumers in purchase and consumption decisions. This study identified various PBMAs in Metro Manila, Bulacan, and Pampanga. Information based on the labels of PBMAs and animal- based meats (ABM) was collected and evaluated. PBMAs (n = 37) included chicken products (n = 4), ground meat (n = 5), patties and sausages (n = 19), jerkies (n = 4), and others [i.e. tocino (Philippine sweet bacon), luncheon meat, and belly rolls]. PBMAs generally have lower energy, total and saturated fat, and higher price/g protein, dietary fiber, sodium, and calcium content compared to ABM. Proteins, carbohydrates, and other micronutrients (i.e. iron, potassium, and vitamin B complex) vary depending on the meat category and ingredient used. Current findings highlight the need to redesign existing PBMAs considering nutritional and economic factors to be at par with ABMs.

USP13 regulates ferroptosis in chicken follicle granulosa cells by deubiquitinating ATG7

The development and maturation of follicles are intricately linked to egg production and reproductive performance of chickens. Granulosa cells death directly affects the development and maturation of follicles, thereby impacting the reproductive performance of hens. Ferroptosis is a new type of cell death, it is unknown how it affects the growth and development of chicken follicles. In this study, RNA-seq analysis revealed significant differences in the expression of ferroptosis-related genes between normal follicles and atretic follicles, suggesting a potential role for ferroptosis in follicle growth and development. In addition, we found that ubiquitin-specific protease 13 (USP13) was significantly upregulated in atrophic follicles. Overexpression of USP13 results in depletion of glutathione (GSH), peroxidation of lipids, accumulation of iron, and activation of ferroptosis in chicken granulosa cells. In contrast, USP13 knockdown significantly inhibited ferroptosis events. Mechanistically, USP13 prevents the degradation of autophagy related 7 (ATG7) by deubiquitinating it, thereby enhancing the stability of ATG7 protein and ultimately promoting ferroptosis. In conclusion, this study elucidates the crucial role of the USP13-ATG7 axis in regulating ferroptosis in chicken follicle granulosa cells, thereby presenting a novel avenue for molecular breeding research in chickens.

Alat Feeding Ayam Otomatis Berbasis Arduino

The increasing population in Indonesia has resulted in increased food needs. One of the factors that affects chicken production is the management of the feeding system. The purpose of this study is to design and create an automatic chicken feeder based on Arduino. Calibrate the Load Cell Sensor. Control the AC motor as a metal screw driver for feeding with a load cell sensor. Determine the amount of chicken feed according to the specified setting point and Connect the MCU Node to the telegram. This study uses the "Research and development" research method. Consisting of literature studies, hardware design, software design, tool design, and data collection, analysis and conclusions. Overall testing was carried out for 2 days starting from 6 am to 4 pm. The capacity of the tank is filled with 3 kg of feed. The setting point for feeding in the morning is 200 grams while in the afternoon it is 300 grams, for feed output there are 2 containers. Testing on the first day at 7 am the weight of the feed coming out of container 1 was 221 grams while in container 2 it was 225 grams, for 4 pm the weight of the feed coming out of container 1 was 352 grams while in container 2 it was 357 grams. Testing on the second day at 7 am the weight of the feed coming out of container 1 was 219 grams while in container 2 it was 221 grams, for 4 pm the weight of the feed coming out of container 1 was 346 grams while in container 2 it was 348 grams. Telegram notification sends a message when the amount of feed remaining is 1405 grams. The results of the weight of the feed coming out are different from the specified setting point value, the amount of feed coming out exceeds the setting point value. This is because the output hole of the feed that comes out through the PVC pipe is too large. An "automatic chicken feed tool based on Arduino" has been successfully created, the tool created is equipped with a load cell sensor that facilitates the process of feeding chickens. Making a system for pouring feed into the hopper automatically by making a conveyor so that it does not need to be done manually. Making automatic chicken feed based on Arduino on a large scale so that it can cover a larger number. Addition of making automatic drinking places on automatic chicken feed based on Arduino.

Acrylamide detection and reduction in meat products using organic acids, fruit extracts, and probiotics

Grilled foods are an important source of acrylamide, which has neurotoxic, genotoxic, and carcinogenic properties. The current study aims to evaluate the level of acrylamide in beef, chicken, and fish products, especially those requiring high cooking temperatures, using High Performance Liquid Chromatography (HPLC). Reduction of acrylamide by organic acids i.e., (citric acid, malic acid, tartaric acid, and lactic acid) and fruit extracts of lemon, apple, and grape has also been investigated. The results revealed that the highest mean acrylamide concentration was found in chicken products (grilled chicken) which recorded 8.32 μg/100 g, followed by beef products (beef grilled) with a concentration of 7.91 μg/100 g, and fish products (pan-fried fish burgers) which recorded 6.77 μg/100 g). Furthermore, the mixture of organic acid has the highest effect on reducing the level of acrylamide in a chemical model system. Moreover, the fruit extract mixture was more effective in reducing the percentage of acrylamide in the grilled chicken than organic acids mixture. Finally, the addition of fruit extract improved the sensory properties of grilled chickens. In sum, this study offers novel and promising natural strategies to decrease acrylamide in meat products toward further future application in meat industry to deliver safe food to consumers.

Research note: effects of hyperpigmentation of the visceral peritoneum on body weight and selection method in Chinese yellow-feathered broilers

We previously identified a dark blue appearance through the skin of abdomen, especially the colored chicken breeds, called hyperpigmentation of the visceral peritoneum (HVP) which characterized by intense pigmentation of connective tissue in the visceral peritoneum. The HVP has recently garnered increasing attention due to its negative impact on carcass appearance, and been an important concern in the poultry industry, especially for the Chinese yellow-feathered broilers. In this study, we measured the in vivo HVP at different time points, and analyzed the correlation between the HVP in vivo and postmortem. Then, established an accurate and reliable HVP phenotypic measuring method in vivo for early selection in chickens and analyzed the association of phenotypic variations with the in vivo HVP traits with growth traits. The results showed that the in vivo HVP at 21 d of age in chickens have a high heritability (h2=0.452) through estimating genetic parameters, and in vivo HVP levels at 21 and 42 d were both significantly associated with those postmortem in chickens, suggesting that directional selection on reducing HVP can be implemented as early as at 21 d in the breeding and production of chickens. Although, we found HVP had no effect on the body weight at 1 d, it could significantly reduce the body weight at 21, 42, 70 d and 91 d in chickens. This suggests HVP not only has a negative effect on carcass traits, but also significantly reduces the production in the poultry industry.

Effects of Moringa oleifera leaf extract on growth performance, blood hematology, immunity, carcass, and meat quality of Mae Hong Son chicken

Moringa oleifera is a nutrient-rich plant with a wide range of beneficial effects on chicken and chicken products. Mae Hong Son chickens, a variety of Thai native poultry, are renowned for their exceptional meat quality, distinct flavor, and remarkable tenderness. Therefore, this present study aimed to investigate the effects of Moringa oleifera leaf extract (MOLE) on growth performance, blood hematology and immunity, carcass, and meat quality of Mae Hong Son chickens, respectively. Briefly, 200 birds of Mae Hong Son chickens were subsequently divided into 4 groups, each containing 50 birds. They were fed for 16 weeks with different supplementary as follows: 1. Control group 2. Placebo (maltodextrin + microcrystalline cellulose PH101) 3. 2% of MOLE extract supplementation and 4. 4% of MOLE extract supplementation. The results showed that T4 achieved the best outcomes in terms of body weight and average daily gain, while also having the lowest total white blood cell (WBC) count and the highest ND HI titer at 12 weeks old (p &lt; 0.05). On the other hand, T1 had the highest fat percentage and thiobarbituric acid reactive substances (TBARS) values in both the thigh and breast (p &lt; 0.05). Additionally, the shear force of T1 was the lowest, both in the thigh and breast (p &lt; 0.05). In summary, the potential of MOLE could improve growth performance, hematological parameters, immune response, and meat quality in Thai native chicken production.

A comparative study of population structure and genetic diversity of commercial and indigenous chickens from different agro-ecological zones in Ghana using SilicoDArT and SNP markers

Chicken production, both in the local and commercial sectors, contributes significantly to human livelihood and food security. Precise use of diverse genetic resources is primary in breeding programs. The study analyzed the genetic diversity and population structure of commercial chickens and indigenous chicken ecotypes from three different agro-ecological zones (Semi-Deciduous Rainforest Zone, Guinea Savannah, and Coastal Savannah) using SilicoDArT and SNP markers, utilizing whole-genome sequencing and phenotypic data. Phenotypic data were collected from 72 indigenous chicken ecotypes across the three AEZs, and 32 commercial birds kept at the Kwame Nkrumah University of Science and Technology (KNUST). DNA samples used for sequencing were obtained from 88 chickens (62 indigenous chicken ecotypes and 26 commercial chickens). A total of 54,995 SilicoDArT and 85,396 SNPs markers were generated from DArTseq genotyping. After filtering, 44,784 SilicoDArT and 58,353 SNP were used for genetic diversity and population structure analysis. Both markers showed high reproducibility and call rate. Polymorphic information content (PIC) values ranged from 0.00 to 0.50, while ≥ 50 % showed PIC values more than the median. Furthermore, we obtained FST values, Nei’s genetic distance, dendrogram analysis, and principal component analysis (PCA) of commercial and indigenous chickens. The FST and Nei’s genetic distance showed that there is high genetic diversity between the commercial chickens and the indigenous chicken ecotypes. However, there was low genetic diversity among the indigenous chicken ecotypes. The PCA analysis indicated a clear separation between the commercial and indigenous chicken ecotypes, while no clear separation was observed between the indigenous chicken ecotypes. The phenotypic data and the dendrogram indicated that naked and frizzle genes do not markedly alter the genetics of indigenous and commercial birds, and their influence on economic traits may be solely determined by the prevailing environmental conditions. The results indicate that there is high genetic differentiation between commercial and indigenous chickens based on SilicoDArT and SNP markers. The indigenous chickens from the agro-ecological zones have low genetic diversity and might have a common origin. Naked neck and frizzle genes do not markedly alter the genetic performance of birds in terms of economic traits. Therefore, the superiority of birds carrying these genes in economic traits may be solely due to environmental variation.

Comparative study of gastrointestinal tract size in three parent breeds for the production of dual-purpose organic chickens.

An alternative to culling male hatchlings of layers is breeding dual-purpose chickens. One breeding objective is the ability to digest low-quality feed. Certain measurements of the gastrointestinal tract may be useful indicators of this ability. The present study compared the gastrointestinal tract of adult hens of two layer-type breeds (White Rock (WR), New Hampshire (NH)) and the meat-type ÖTZ (Ökologische Tierzucht gGmbH) Bresse Gauloise (BR), used for the production of dual-purpose organic chickens. Flocks had the same housing and feeding conditions. At slaughter at 19 months, the body weight and gastrointestinal organs of 134 hens (51 WR, 55 NH and 28 BR) were measured. The muscle thickness of the proventriculus and ventriculus and the length and width of the duodenum, jejunoileum, caeca and colorectum were measured and variances between the groups were analysed using a one-factor covariance analysis. Significant differences between the breeds were found in total intestine length and the lengths and/or widths of single gastrointestinal segments. For example NH showed the highest mean total intestinal length and BR showed the lowest mean (NH: 186.73 cm, WR 185.86 cm, BR 157.91 cm; p = 0.001). To our knowledge, this is the first study comparing the gastrointestinal tract size of adult female layer- and meat-type chicken breeds kept under the same feeding and housing conditions. Given the possible relationship between intestinal length parameters and chicken performance, measurement of the gastrointestinal tract may be a simple, quick and inexpensive additional method to help select layer-, meat-type and dual-purpose chickens suitable for organic production when a selection process using genetic markers is not possible.

Transition to Whole Genome Sequencing Surveillance: The Impact on National Outbreak Detection and Response for Listeria monocytogenes, Salmonella, Shiga Toxin-Producing Escherichia coli, and Shigella Clusters in Canada, 2015-2021.

Between 2017 and 2019, pulsed-field gel electrophoresis was replaced by whole genome sequencing (WGS) for identifying enteric disease clusters in Canada. The number and characteristics of all clusters of Listeria monocytogenes, Salmonella, Shiga toxin-producing Escherichia coli (STEC), and Shigella spp. between 2015 and 2021 were analyzed. Following the transition to WGS, an increase in the number of Salmonella, STEC, and Shigella clusters was noted, whereas the number of clusters of L. monocytogenes decreased. Unlike previous subtyping methods, WGS provided increased resolution to identify discrete clusters of Salmonella Enteritidis. This led to the identification of a number of outbreaks linked to frozen raw breaded chicken products and ultimately a change in food safety policy to reduce the number of illnesses associated with these products. Other pathogens did not experience a similar increase in the number of outbreaks detected. Although WGS did provide increased confidence in the genetic relatedness of cases and isolates, challenges remained in collecting epidemiological data to link these illnesses to a common source.

Effect of High Dietary Iron on Fat Deposition and Gut Microbiota in Chickens.

To meet the demand of consumers for chicken products, poultry breeders have made improvements to chickens. However, this has led to a new problem in the modern poultry industry, namely excessive fat deposition. This study aims to understand the effects of dietary iron supplementation on fat deposition and gut microbiota in chickens. In this study, we investigated the effects of iron on the growth performance, fat deposition, and gut microbiota of silky fowl black-bone chickens. A total of 75 7-week-old silky fowl black-bone chickens were randomly divided into three groups (five replicates per group, five chickens per replicate) and fed them for 28 days using a growing diet (control group), a growing diet + 10% tallow (high-fat diet group, HFD group), and a growing diet + 10% tallow + 500 mg/kg iron (HFDFe500 group), respectively. We detected the effects of iron on the growth performance, fat deposition, and gut microbiota of silky fowl black-bone chickens using the growth performance index test, oil red O staining, and HE staining, and found that the high-fat diet significantly increased liver and serum fat deposition and liver injury, while the addition of iron to the diet could reduce the fat deposition caused by the high-fat diet and alleviate liver injury. In addition, 16S rDNA sequencing was used to compare the relative abundance of gut microbiota in the cecal contents in different feeding groups. The results showed that the high-fat diet could induce gut microbiota imbalance in chickens, while the high-iron diet reversed the gut microbiota imbalance. PICRUSt functional prediction analysis showed that dietary iron supplementation affected amino acid metabolism, energy metabolism, cofactors, and vitamin metabolism pathways. In addition, correlation analysis showed that TG was significantly associated with Firmicutes and Actinobacteriota (p < 0.05). Overall, these results revealed high dietary iron (500 mg/kg) could reduce fat deposition and affect the gut microbiota of silky fowl black-bone chickens, suggesting that iron may regulate fat deposition by influencing the gut microbiota of chickens and provides a potential avenue that prevents excessive fat deposition in chickens by adding iron to the diet.

Measuring urofecal glucocorticoid metabolites in broiler chicken: a noninvasive tool for assessing stress as a marker of welfare

The poultry industry is an important and still growing sector in many parts of the world. For ethical reasons and due to increased consumer awareness for animal welfare in production animals, it is of importance to establish a reliable and objective test system for monitoring and improving health and welfare. During the rearing process, broiler chickens are exposed to numerous potential stressors and management interventions (e.g. weighing of individual animals, preslaughter fasting and capture processes), but assessing the level of stress perceived by the animals entirely through behavioral observations can be challenging. Monitoring stress-related physiological markers, such as glucocorticoids, can be an accurate and presumably more objective addition. To avoid additional stressors induced by blood collection, a noninvasive approach using urofecal samples is advisable. However, a thorough validation is needed to establish a suitable test system for measuring stress hormone levels, including potential effects of the time of day of collection or the time that has elapsed since defecation. Therefore, the aim of this study was to test the stability of urofecal glucocorticoid metabolites (ufGCM) postdefecation, to determine time of day effects on ufGCM levels, and to investigate the effect of standard management procedures on ufGCM concentrations in broiler chickens. Our results revealed a time window of 4 h in which fecal samples from broilers can be collected without major alterations to the ufGCM concentrations. In this regard, a "fecal box" proved useful for collecting uncontaminated fresh samples. The time of day of sample collection did not influence ufGCM concentrations significantly. Moreover, the used assay proved to be sensitive enough to detect even small and short-lasting activations of the HPA axis induced by handling, confinement, and fasting. Thus, the system used can be a powerful and easy to apply tool in a chicken production setup for assessing stress as a marker of welfare in commercially housed broiler chickens, which in the long-term can also improve production, particularly with regard to process quality.