Production Performance Research Articles

Optimizing growth conditions and biostimulant application for enhanced growth, yield and quality in Butter bean (Phaseolus lunatus L.) cultivar KKL 1

Butter beans (Phaseolus lunatus) are prized for their significant nutraceutical benefits, which are crucial in addressing malnutrition in developing countries. Given their nutritional advantages, global cultivation demand is increasing. This study aims to boost production and improve crop quality through effective agronomic practices. To minimize dependence on synthetic chemicals, the study investigates the use of biostimulants as an alternative, offering a promising method for enhancing crop growth and performance. The experiment took place under 2 different conditions: polyhouse (G1) and open field condition (G2) at Horticultural and Forestry Research Station, Kodaikanal, from December 2023 to June 2024. Various biostimulants were applied in foliar parts in different doses: control (B1) seaweed extract at 3 % (B2) and 5 % (B3), panchagavya at 3 % (B4) and 5 % (B5), vermiwash at 5 % (B6) and 10 % (B7) and fulvic acid at 6 g/L (B8) and 9 g/L (B9), each replicated 3 times. Among the treatment combinations, G1B3 (seaweed extract 5 % under polyhouse condition) was most effective in all growth, yield and quality parameters including days to first flower emergence (48.50 days), number of pods per plant (96.33), pod yield (4.98 t/ha), highest soluble protein (37.36 %) than G2B1 (control group under open field condition). It resulted in a greater number of days for flower emergence (57.85 days), lowest values in number of pods per plant (69.91), pod yield (2.96 t/ha) and soluble protein (24.69 %). These findings suggest that seaweed extract has great potential as a sustainable agricultural input, enhancing productivity and crop performance in butter bean cultivation. The main goals were to investigate how different biostimulants and growing conditions affect the growth and yield of butter beans and to identify effective organic growth promoters that could improve the yield and quality of butter bean crops in various growing environments. The results of this study are discussed in the following sections.

Effect of stocking density on growth performance, survival and production of Monosex Tilapia (Oreochromis niloticus) under nursery ponds in northern regions of Bangladesh

The present study was to assess and evaluate the culture potential of Tengra with short-cycle species in the seasonal water bodies of farmers’ fields and disseminate the cultural technologies in a different part of the country. The research study was evaluating the improvement of nursery management technique in drought prone area of Northern region in farmer field level. Before conducting research fish farmers of Different upazila were selected by departmental authority and pre training management. Growth, survival and production performances of Oreochromis niloticus fingerlings were evaluated for 40 days in nine nursery ponds having an area of 20 decimal each with an average depth of 1.0 m. Three stocking densities such as 1000 dec-1 (T1=Treatment-1), 1200 dec-1 (T2= Treatment-2) and 1400 dec-1 (T3=Treatment-3) were tested with three replications. Fry were fed with commercial nursery feed (30% crude protein). The rate of feeding was 25% to 8% of the estimated body weight of fry. The physico-chemical and biological parameters of pond water were within the suitable range for fish culture. Growth in terms of final weight, final length, weight gain, length gain and specific growth rate was significantly higher in T1 than those obtained from T2 and T3. The survival of fish fingerlings was higher in T1(85.67%) than T3 (76%) and T2 (83%) respectively. Maximum of fingerlings was produced in T2 than those in T1 and T3. Among the treatments evaluated, 1000 dec-1 was the best stocking density considering the growth performance of the fingerlings of monosex Tilapia, O. niloticus in nursery ponds.

Malva sylvestris leaf powder as a feed additive affects the performance, carcass traits, meat quality attributes, serum antioxidants, stress physiology, intestinal bacterial counts, and gut morphology of broiler chicken.

This study investigated the effect of supplementation of Malva sylvestris leaf powder (MSLP) on the production performance of broiler chicken. Ven Cobb broiler chicks (240 day-old male chicks) were distributed randomly into four treatments, each replicated four times, with 15 birds per replicate. The diets formulated were T1 (control) given basal diet only, T2 (basal diet +1.0% MSLP), T3 (basal diet +1.5% MSLP), and T4 (basal diet +2.0% MSLP). The highest improvement of 3.83% in the average daily gain (ADG) was recorded in the T3 group fed 1.5% Malva powder in the diet compared to the control (P = 0.009). The average daily feed intake (ADFI) tended to decrease with an increase in the dose of MSLP in the diet, with the lowest feed intake in the T4 group fed 2% MSLP. During the overall period (7-42days), the feed/gain (F/G) ratio reduced significantly (P = 0.048) in the T3 and T4 groups compared to the control. The dressed and breast meat yield was found to be significantly (P < 0.05) higher in the T3 group, with no significant change (P > 0.05) in the thigh yield. The changes in the pH and water-holding capacity (WHC) of breast meat were found to be non-significant (p > 0.05) between the control and various other treatments. Thiobarbituric acid-reactive substances (TBARS) were significantly (P < 0.05) decreased in the T3 and T4 groups. There was no negative effect of including MSLP in the diet on the color coordinates of breast meat among different treatments. Compared to the control, the serum immunoglobulin values increased significantly (P < 0.05) in the T3 and T4 groups. Superoxide dismutase (SOD) showed no difference between various treatments; however, malondialdehyde (MDA) decreased significantly (P < 0.05) according to dietary treatments. Serum cortisol increased significantly (P < 0.05) in the T4 group compared to other treatments. The inclusion of Malva powder in the diet at the 2% level significantly (P < 0.05) decreased the coliform count compared to the control birds. Supplementation with Malva powder resulted in a significant (P < 0.05) increase in the villus height-to-crypt depth (VH:CD) ratio of broiler birds in the T3 and T4 groups. In conclusion, MSLP supplementation at 1.5% and 2% resulted in improved production performance of broiler chicken.

Nitrogen Fertilization Using Conventional and Slow-Release Fertilizers at Multiple Levels in Lolium multiflorum Lam. Pastures

Pastures are essential for sustaining dairy production, particularly in temperate climates where year-round grazing is feasible. However, comprehensive analyses of their productivity, efficiency, and cost evaluation remain uncommon. This study evaluated the productivity, quality, nitrogen fertilization use efficiency, and production costs of Lolium multiflorum Lam pasture on a farm in São Miguel Island. The research compared conventional nitrogen fertilizer with slow-release nitrogen fertilizer at application rates of 320 and 160 kg N ha−1, alongside a control treatment. A Latin square design with five treatments was employed to assess both agronomic and economic performance. The results showed that the type of fertilizer did not significantly influence productivity or quality, while nitrogen levels had a notable impact. Higher nitrogen doses increased dry matter yield and crude protein content. Fiber characteristics remained relatively stable, though neutral detergent fiber and acid detergent fiber levels tended to rise with increased nitrogen application. Nitrogen fertilizer use efficiency consistently exceeded 70%, regardless of the fertilizer type or application rate. Regarding production costs, conventional fertilizer was significantly more cost-effective than slow-release fertilizer, underscoring the importance of selecting economically viable options without compromising agronomic performance in forage production.

Engineering lipase TLL to improve its acid tolerance and its biosynthesis in Trichoderma reesei for biodiesel production from acidified oil

Lipases catalyze the synthesis of biodiesel, which is an important renewable alternative energy source. Cost-efficient conversion of waste acidified oil to biodiesel entails acid-tolerant lipases which have not been extensively studied. This study showed that the commonly used Thermomyces lanuginosus lipase TLL displayed a weak acid tolerance and an unsatisfactory performance in biodiesel production from acidified oil. Directed evolution of TLL identified one TLL-T3 variant with three residue substitutions (A69S/V150P/N222G). TLL-T3 displayed significantly enhanced acid tolerance, and its application in acidified oil treatment led to a biodiesel yield up to 90 % (w/w). A scaled-up production of TLL-T3 in Trichoderma reesei was further achieved and the highest extracellular lipase activity reached 16,123 U/mL after fermentation optimization. These results provide new insights into structural adaptation to acid tolerance by lipases and show that TLL-T3 holds great potential in commercial biodiesel production from waste acidified oil.

Quantitative analysis of gametic imprinting effects on productive and reproductive performances of Markhoz goat

In this study, in order to assess the relative importance of gametic imprinting effects for the genetic variation of growth and reproduction traits of Markhoz goat, a data set were obtained from a total of 4983 individuals on productive and reproductive traits of this breed (collected from 1993 to 2017) was analysed. The studied productive traits included body weight (BW) of Markhoz kid at birth and 4 (WW), 6 (W6), 9 (W9), and 12 (W12) months of age, average daily weight gains (g day−1) in the first two growth phases (i.e. from birth to weaning, ADG0–4; and from weaning to 6-month age, ADG4–6) and their corresponding Kleiber ratios (i.e. KR0–4 and KR4–6). In addition, reproduction traits of interest were the liter size at birth (LSB) and at weaning (LSW), total litter weight at birth (TLWB) and at weaning (TLWW) per does kidding, litter mean weight per kid born (LMWKB) and per kid weaned (LMWKW). Likelihood ratio tests and Akaike’s Information criterion indicated that gametic imprinting effects were significant only for BW, W9 and KR0–4. Both paternal and maternal gametic imprinting effects had a similar influence on these traits, accounting for between 8 % (W9) and 10 % (BW and KR0–4) of the total phenotypic variance. For these traits the model with the second-best fit was models including paternal gametic imprinting effects, which indicates crucial paternal gametic imprinting effects in the analyzed traits in the production context. For parentally gametic influenced traits of Markhoz kid, including these effects significantly decreased the direct additive genetic variances (20–94 % depending on the trait) and these reduction were higher when paternal and maternal gametic variance evaluated together than when evaluated separately. Generally these results indicate that when gametic imprinting effects are of importance, but not accounted for, heritability estimates are biased upward and subsequently the realized efficiency of selection is reduced. It was concluded that for more precise estimates of genetic parameters, extracting of overly influential observations from the dataset is necessary.

Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae

The efficient production of biobased organic acids is crucial to move to a more sustainable and eco-friendly economy, where muconic acid is gaining interest as a versatile platform chemical to produce industrial building blocks, including adipic acid and terephthalic acid. In this study, a Saccharomyces cerevisiae platform strain able to convert glucose and xylose into cis,cis-muconic acid was further engineered to eliminate C2 dependency, improve muconic acid tolerance, enhance production and growth performance, and substantially reduce the side production of the intermediate protocatechuic acid. This was achieved by reintroducing the PDC5 gene and overexpression of QDR3 genes. The improved strain was integrated in low-pH fed-batch fermentations at bioreactor scale with integrated in situ product recovery. By adding a biocompatible organic phase consisting of CYTOP 503 and canola oil to the process, a continuous extraction of muconic acid was achieved, resulting in significant alleviation of product inhibition. Through this, the muconic acid titer and peak productivity were improved by 300% and 185%, respectively, reaching 9.3 g/L and 0.100 g/L/h in the in situ product recovery process as compared to 3.1 g/L and 0.054 g/L/h in the control process without ISPR.

Effect mechanism of Cd on band structure and photocatalytic hydrogen production performance of ZnS

ZnS is widely used in the photocatalytic decomposition of water to produce hydrogen due to its fast electron-hole pair generation and high negative potential. However, its absorption in the visible region is poor due to its wide band gap, and it has serious photogenerated carrier recombination problems. Herein, a shallow impurity energy level was introduced by doping the ZnS lattice with Cd. Due to its presence, electrons trying to return to the valence band are trapped and excited twice, suppressing the recombination of photogenerated carriers and greatly improving electron utilization. The Cd1.5-ZnS possesses a hydrogen production rate as high as 85722.20 μmol/g, which is 17 times higher than pure ZnS. Meanwhile, Cd1.5-ZnS has a narrower forbidden band and superior visible light absorption, and the serious photocorrosion problem of ZnS has been suppressed. This study provides a viable approach for the synthesis of photocatalysts with adjustable band gaps and enhanced hydrogen precipitation efficiency.

Label-Free Exosome Analysis by Surface-Enhanced Raman Scattering Spectroscopy with Laser-Ablated Silver Nanoparticle Substrate.

Early diagnostics of breast cancer is crucial to reduce the risk of cancer metastasis and late relapse. Exosome, which contains distinct information of its origin, can be the target object as a liquid biopsy. However, its low sensitivity and inadequate diagnostic tools interfere with the point-of-care testing (POCT) of the exosome. Recently, Surface-enhanced Raman Scattering (SERS) spectroscopy, which amplifies the Raman scattering, has been proved as a promising tool for exosome detection. However, the fabrication process of SERS probe or substrate is still inefficient and far from large-scale production. This study proposes rapid and label-free detection of breast cancer-derived exosomes by statistical analysis of SERS spectra using silver-nanoparticle-based SERS substrate fabricated by selective laser ablation and melting (SLAM). Employing silver nanowires and optimizing laser process parameters enable rapid and low-energy fabrication of SERS substrate. The functionalities including sensitivity, reproducibility, stability, and renewability are evaluated using rhodamine 6G as a probe molecule. Then, the feasibility of POCT is examined by the statistical analysis of SERS spectra of exosomes from malignant breast cancer cells and non-tumorigenic breast epithelial cells. The presented framework is anticipated to be utilized in other biomedical applications, facilitating cost-effective and large-scale production performance.

Mathematical modeling for the production performance of cyclic multi-thermal fluid stimulation process in layered heavy oil reservoirs

Multi-thermal fluid (MTF), with a unique thermophysical characteristics, has been widely applied in the enhanced heavy oil recovery processes. Especially for the layered heavy oil reservoirs, MTF shows more attractive than the conventional saturated steam. In this study, we propose an improved mathematical model for the production performance of cyclic MTF stimulation process in layered heavy oil reservoirs. In this model, based on a three-zone assumption, we first derive a productivity model for the thermal-fluids huff-n-puff process in layered heavy oil reservoirs. Thereafter, considering the typical EOR mechanisms of non-condensable gases in MTF, the viscosity reduction effect caused by CO2 dissolution and the effect of formation energy supplement caused by N2 are mathematically described. Simultaneously, because of the nonlinear temperature spreading behavior in the formation, an MTFs flooding experiment is also performed to characterize the effect. Meanwhile, a heating radius model with the consideration of steam overlap effect is also involved in our model. Then, the calculation results are compared against the results of a commercial simulator to confirm the accuracy, while a sensitivity analysis on the reservoir and operation parameters is performed. This paper provides a quick estimation approach for the productivity of cyclic MTF stimulation process in layered heavy oil reservoirs.

Estimation of Genetic Parameters for Milk Production Rate and Its Stability in Holstein Population.

Milk production rate (MPR) refers to the rate of milk secretion per hour (kg/h), calculated from the harvested milk yield and milking interval, and it is considered an appropriate measure to evaluate the production potential of cows. The objective of this study was to estimate the phenotypic and genetic parameters of milk production rate traits. In this study, the milking records of 4760 Holstein cows were collected, and four milk yield traits and six milk production rate traits were defined. The MIXED procedure was used to detect the impacts of non-genetic effects on milk yield and milk production rate traits, including parity, measured season and lactation stage. Variance and covariance components for milk yield and milk production rate traits were estimated using a univariate linear repeatability model. Parity, measurement season and lactation stage had significant effects (p < 0.01) on milk yield, milk production rate and its stability. Milk yield and milk production traits had high heritability, and ranged from 0.25 to 0.39. The stability of milk production rate had low heritability (0.04~0.05). Milk production rate is beneficial for the devolving novel trait in dairy breeding and provides new insights for herd management and genetic selection of production performance of dairy cattle.

Production performance and water quality of Caulerpa lentillifera with fermented fruit vegetable fertilizer

The use of fermented vegetable and fruit waste (FVF) based fertilizers as an effort to reduce waste has the potential to be used to support aquaculture activities. This study aims to evaluate the effectiveness of Caulerpa lentillifera cultivation with the addition of fertilizer based on fruit and vegetable waste fermentation on water quality parameters and production performance. The experimental design used was a completely randomized design with three replications for four different doses of FVF fertilizer treatments, namely 0, 0.05, 0.1, and 0.2 µl/L. This study used an initial weight of 500 g which was reared in a concrete tank with a capacity of 1000 L for 30 days growth period. The results showed that the use of FVF of 0.1 µl/L gave a better net harvest weight (p&lt;0.05), namely 0.08 ± 0.06 kg. Keywords: Caulerpa lentillifera, fermentation, fertilizer, seaweed, waste ABSTRAK Penggunaan pupuk berbasis fermentasi limbah sayur dan buah (FVF) sebagai salah satu upaya pengurangan limbah memberikan potensi sebagai larutan pendukung kegiatan budidaya. Penelitian ini bertujuan untuk mengevaluasi efektivitas budidaya Caulerpa lentillifera dengan penambahan pupuk berbasis fermentasi limbah buah dan sayur terhadap parameter kualitas air dan kinerja produksi. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan tiga kali ulangan pada empat perlakuan pupuk, yaitu 0, 0.05, 0.1, dan 0.2 µl/L. Penelitian ini menggunakan bobot awal 500 g yang dipelihara dalam bak beton berkapasitas 1000 L. Hasil pengamatan menunjukkan bahwa penggunaan FVF sebesar 0.1 µl/L memberikan bobot panen bersih yang lebih baik (p&lt;0.05) yaitu 0.08 ± 0.06 kg. Kata kunci: Caulerpa lentillifera, fermentasi, limbah, pupuk, rumput laut

Effect of charcoal balls on distillation productivity in hemispherical solar stills: Experimental investigation and performance analysis

This study investigates the enhanced performance of hemispherical solar stills by integrating charcoal balls. The method showcases improved efficiency by augmenting heat transfer mechanisms and enhancing solar radiation absorption. Reducing the surface tension of basin water is a solution to increase its heating, enhancing the softwater yield of solar stills. The novelty of the research lies in the innovative integration of charcoal balls to improve the performance of hemispherical solar stills. This method presents a unique approach to enhancing heat transfer and evaporation dynamics, potentially revolutionizing the efficiency of solar desalination systems. Therefore, the impact of charcoal balls at a diameter of 1 cm on the performance of distillation productivity was studied. A hemispherical solar still containing charcoal balls (HSS-CB) was compared with a simple hemispherical solar still (SHSS) in the same conditions. Experiments were conducted at El-Oued-Algeria in June 2024. The method employed in this research offers distinct advantages, primarily in its ability to enhance solar still efficiency by incorporating charcoal balls. These advantages stem from the increased surface area for heat transfer, improved solar radiation absorption, and potential enhancement of evaporation and condensation processes. Cumulative return results for SHSS and HSS-CB are 4.80 and 6.20 L/m2/day, respectively. The rate of improvement in the cumulative return was 29.16 % for SHSS using the quantity of coal powder. It is recommended that charcoal balls be used to boost solar stills productivity.

A Meta-analysis of the Profit-Sharing Practices among Engineering, Construction, and Related Firms: Strategy to Increase Company Revenue

This paper explores the impact of profit sharing on employee motivation, productivity, and company performance in the engineering and construction industry. Through a meta-analysis of recent studies, it identifies key factors that influence the effectiveness of profit-sharing programs. The findings reveal that profit sharing significantly boosts employee motivation and productivity, with increases of 15% and 12% respectively. Moreover, job satisfaction rates are 20% higher among employees participating in profit-sharing programs. However, there is a notable preference split, with 60% of employees favoring profit sharing for its potential higher earnings, while 40% prefer fixed salary increases for stability. The research further indicates that frequent and actively managed profit-sharing distributions, such as quarterly payouts, lead to better financial performance, with a 10% increase in overall performance and a 5% improvement in profitability. Project-based profit sharing in the construction sector is particularly effective, resulting in a 25% increase in project completion rates and a 15% reduction in project costs. Additionally, profit sharing is more effective in unionized organizations, with an 18% increase in effectiveness, and mandatory profit-sharing frameworks enhance worker satisfaction and productivity. Based on these findings, the paper recommends developing tailored profit-sharing strategies that cater to different employee preferences, implementing frequent and active management of profit-sharing distributions, adopting project-based profit sharing in project-centric industries, engaging with unions for fair terms, advocating for supportive regulatory frameworks, and continuously monitoring and evaluating the effectiveness of these programs. By adopting these strategies, engineering and construction firms can enhance employee motivation, improve productivity, and achieve better financial performance, driving organizational success.

Experimental investigation of distilled water production performance of conventional solar stills using CaCl2·6H2O phase change material reinforced with SrCl2·6H2O and graphene-based nanoparticles

This study aims to enhance the performance of conventional single-slope basin-type solar stills by integrating phase change materials (PCMs) and nanoparticles. Calcium chloride hexahydrate, known for its high heat storage capacity, was selected as the primary PCM. Strontium chloride hexahydrate was added as a nucleating agent to address the supercooling tendency. Additionally, graphene oxide (GO) and graphene nanoplatelets (GNP), which have not been previously used in solar stills or compared for their performance, were incorporated into the PCM to utilize their photothermal properties and enhance solar energy absorption. The integration of GO and GNP with the PCM-nucleating agent combination resulted in daily total distilled water productions of 5424ml/m2 and 5032 ml/m2, respectively. The integration of GO with the PCM-nucleating agent combination provided the most significant improvement in efficiency, with a 54.44 % increase compared to the standard still, a 15.4 % increase compared to the still using only the nucleating agent, and an 8 % increase compared to the still incorporating GNP.

Boosting photoelectrochemical hydrogen production performance by in-situ transformation of self-assembled Cd(OH)2 nanowire on CdS nanorod

In this work, solvothermal methods were used to create CdS nanorods (NRs) on Cd foil. Furthermore, a second hydrothermal procedure was performed in water to improve the photoelectrochemical (PEC) properties of the base material at different time variations (3, 6, and 12 h at 160 °C). After the second hydrothermal process, self-assembled Cd(OH)2 NWs were grown on CdS NR. The optimized Cd(OH)2 NW/CdS NR-6 h photoanode exhibited a higher photocurrent density of 5.7 mA/cm−2 at 0 V vs. Ag/AgCl under one sun illumination. Also, the optimum Cd(OH)2 NW/CdS NR-6 h photoanode showed a hydrogen evolution rate of 216 μmol cm−2 for 3 h. The enhanced PEC performance is mainly attributed to the removal of organic moieties, the partial transformation of self-assembled Cd(OH)2 NWs to CdS NR during PCE measurement, and provided effective charge separation and delayed the recombination. We believe that the findings of this study will provide guidelines for designing and fabricating sunlight-driven photoanodes for the PEC hydrogen production system.

Synergistically achieving particulate matter reduction and production performance optimization for zinc electrolysis by ultrasonication coupling anode-coated MnO2

Abating particulate matter (PM) from electrolysis processes is significant because this PM poses occupational threats to workers and has a negative impact on air quality. However, it remains a challenge to synergize the reduction of PM production and the improvement of electrolysis performance. This study developed a green method, termed as the coupling of ultrasonication and pre-coating MnO2 anode treatment (UMT). The effects on PM generation rate and electrolysis performance indicators were estimated using the bench-scale zinc electrolysis device, and the synergistic mechanism of UMT was expanded from the perspective of electrochemical reactions and bubble characteristics using analysis of reaction products, camera technique, and PM generation prediction models. The results showed UMT not only overcame the degradation of deposited zinc quality caused by the ultrasonication treatment but also solved the increased PM generation by the pre-coating MnO2 film treatment. The UMT simultaneously reduced PM (33.9 %-57.5 %), decreased zinc impurity content by (11.2 %-54.3 %), improved current efficiency of zinc deposition (0.19 %-1.71 %), and conserved electrolysis energy (0.27 %-1.01 %). The optimal performance of UMT occurred at 80 kHz. The UMT suppressed the gas evolution reactions and prematurely bursting bubbles, in favor of reducing the number and size of bubbles, to reduce PM generation. Meanwhile, the UMT improved the electrolysis performance by inhibiting the corrosion of lead-based anodes, promoting the mass transfer rate of Zn2+, providing more active surfaces, and decreasing the overpotential of reactions. The findings may provide references for the green development of the metal electrolysis industry.

Enhancing laying performance and immunity via probiotic and vitamin additives during induced molting.

Molting is induced in commercial laying hens to rejuvenate the reproductive system and increase egg production. However, this process causes stress and reduces bird health and performance. The experiment was conducted to study the effect of multi probiotics and vitamin additives on induced molting in 240 ISA Brown hens. Hens were randomly divided into four groups receiving probiotic and vitamin additives (I-IV) during different period of molting. During the whole molting process, the laying performance indexes such as egg laying rate, egg quality, ovary weight and oviduct lengths were measured, and the spleen index, serum immunoglobulin, immune response of NDV and AIV vaccine were monitored. Molted hens resumed 50% egg production in just 37 days, with 1.62% mortality. Egg quality such as egg weight, yolk color, Haugh unit, eggshell strength and protein height were significantly improved. After the second production peak, the reproductive organs and immune organs returned to normal, and the immune antibody titer of NDV vaccine increased significantly. Molting with probiotic and vitamin additives improve the laying performance and egg quality, reduce mortality, significantly improve immune function and vaccine titer, and help to enhance disease resistance and maintain production performance of aged laying hens.

Shifting feed delivery time: effects on feeding behavior, milk production, and blood biomarkers in late-lactating dairy cows

Improving the synchronization between the pattern of milk synthesis and nutrient availability throughout the day could enhance production efficiency. In this study, we evaluated the effects of changing feed delivery time on milk production, feeding behavior, and the daily rhythms of blood biomarkers. Eight multiparous Holstein cows housed in a tie-stall barn with controlled environmental conditions were enrolled in a crossover experimental design with three periods of 14 days and three treatments each. Cows were milked twice daily (0530 and 1730 h) and were individually fed with two equal meals of forage and eight equal meals of concentrate during the day. Forage meals were provided at 12-hour intervals either: (i) 5 h before each milking (0030 and 1230 h; BM), (ii) at the end of each milking (0530 and 1730 h; ME), (iii) or 2 h after (0730 and 1930 h; AM). Feed intake and feeding behavior were monitored, and milk production and composition were measured. Blood samples were collected every 4 days at 0700 h and, during the last day of each period, 15 times daily to determine metabolic profiles, hormones, and their daily rhythmicity by the cosinor analysis. Changing forage delivery time did not affect milk yield and dry matter intake. No difference was observed in feeding behavior when expressed relative to the first meal. There were no significant differences in milk component contents and yields. In samples collected at 0700 h, ME had reduced plasma calcium (Ca), magnesium (Mg), and potassium (K) and increased sodium (Na). AM had increased inflammation, as suggested by the greater blood globulin and ceruloplasmin. The patterns of metabolic biomarkers had limited variations when expressed relative to the first forage meal. Nevertheless, the daily rhythms of these biomarkers were remarkably different. Under our conditions, feeding forage meals to cows at different times of the day did not influence productive performance but highlighted the importance of considering the sampling time when interpreting metabolic profiles.

Possibilities of Changes in Energy Intensity of Production Depending on the Scale of Farm Investments in a Polish Region

The purpose of this paper was to analyze the possibility of changes in energy intensity of production in the context of farm investment scale. The empirical section relies on unpublished FADN microdata. The study answers the question of whether investments and fixed capital growth can contribute to improvements in environmental performance of agricultural production. As it turns out, the group of farms with the greatest amount of investments saw an increase in energy consumption costs, though at a much slower rate than growth in production value. As a result, there was a drop in energy intensity of production, defined as the ratio between energy costs and production value. These findings corroborate the hypothesis advanced in this study, namely that upon reaching a sufficiently large amount of investments, farms can become capable of reducing the energy intensity of their production activity. Hence, higher levels of capital productivity are attained when the farms invest in more energy-efficient fixed assets. Conclusions cannot be universal, as the analysis involved a portion of selected farms. However, the research indicates a trend in the study of energy intensity of production depending on the scale of investment and the validity of the investment comprehensiveness approach. These conclusions provide a basis for recommendations for an agricultural policy which should include support for investments that are large and comprehensive (in relation to the farm’s assets) and go beyond the simple renewal of assets. Therefore, in its investment-oriented measures, the agricultural policy should take the innovativeness (including energy efficiency) criterion into account.