Production Performance Of Dairy Cows Research Articles

Role of mineral mixture supplementation in enhancing productivity and profitability of peri-urban dairy farming

A study of 3 months duration was conducted in coastal belt of Odisha to assess the effect of supplemental mineral mixture on productive performance of lactating crossbred cows. Twenty crossbred cows were divided into two groups (T1 and T2) consisting of ten animals each. Animals in group T1(control) were fed on homemade concentrate mixture in fodder based diet consisting of concentrate and roughage (40:60), whereas the animals in group T2 were additionally supplemented with mineral mixture at 2% of concentrate mixture. Average daily dry matter intake (DMI), milk yield, milk composition were recorded at fortnight intervals during the trial. A digestibility trial of six days duration was conducted at the end of experimental feeding. DMI was not influenced by mineral mixture supplementation. Apparent digestibility of crude protein and ether extract were higher (P<0.05) in mineral mixture supplemented group than control group. Supplementation of mineral mixture improved the serum mineral level and improved milk yield by 18%. It was concluded that dietary supplementation of mineral mixture improved nutrient utilization, mineral bioavailability and milk production performance in dairy cows fed fodder based diet and enhanced income of farm families by 30%.

Potential hemo-biological identification markers to the left displaced abomasum in dairy cows

BackgroundLeft displaced abomasum (LDA) occurs at high frequency in the early postpartum period and can affect production performance of dairy cows. Clinical diagnosis of LDA is usually done by abdominal auscultation and percussion. The purpose of this study was to explore the potential applicability of blood biomarkers for early warning and diagnosis of LDA in dairy cows.ResultsTwenty early postpartum healthy cows and thirty early postpartum LDA cows of similar parity were used. A receiver operating characteristic curve (ROC) method was used to analyze the sensitivity of hematological biomarkers to LDA including energy balance metabolic biomarkers, liver/kidney function biomarkers, and minerals. A cut-off point was defined for each of the selected hematological biomarkers deemed sensitive markers of LDA. Compared with healthy cows, body condition score (BCS), dry matter intake (DMI) and milk production were lower in LDA cows. Among energy metabolism markers, serum non-esterified fatty acid (NEFA), β-hydroxybutyric acid (BHBA), insulin (INS), and revised quantitative insulin sensitivity check index (RQUICKI) levels were lower while serum glucagon (GC) was greater in LDA cows. Among the liver/kidney function biomarkers, activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH), the ratio of AST/ALT and levels of total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), albumin (ALB), blood urea nitrogen (BUN), creatinine, and total protein (TP) were greater in LDA cows. Among minerals analyzed, serum Cl, Ca, and K were lower in LDA cows. After ROC analysis, it was determined that serum Ca, INS, RQUICKI, ALT, GGT, and creatinine are potential indicators for early warning and diagnosis of LDA for early postpartum dairy cows.ConclusionsDairy cows with LDA were under severe negative energy balance (NEB), had signs of liver damage and potentially lower insulin sensitivity. A combination of multi-hematological biomarkers including Ca, INS, RQUICKI, ALT, GGT and creatinine has the potential to help identify cows at risk of LDA in the early postpartum period.

Rumen-bypassed tributyrin alleviates heat stress by reducing the inflammatory responses of immune cells

Heat stress (HS) in summer will seriously affect the health and performance of dairy cows. To alleviate the injury to dairy cows caused by HS, we added the rumen-bypassed tributyrin to the feed. We determined whether cows were in a heat-stressed environment by testing the temperature humidity index in the morning, at noon, and in the evening. The detection of anal temperature and respiratory frequency further proved the HS state of the dairy cows. The quantificational real time PCR results showed that tributyrin could significantly reduce the relative expression of tumor necrosis factor α, interleukin 1β, and Interleukin 6. Western blot results showed that tributyrin could alleviate the lymphocyte inflammatory response by inhibiting the mitogen-activated protein kinase and nuclear factor-кB signaling pathways. To further detect the effect of tributyrin on HS in dairy cows, routine biochemical and blood tests were carried out. The results showed that the contents of aspartate aminotransferase, total bilirubin, creatinine, albumin, and globulin were significantly reduced by tributyrin. The results showed that tributyrin could significantly alleviate the liver and kidney injury induced by heat stress in dairy cows. Moreover, tributyrin could also significantly reduce the numbers of intermediate cells and increase the level of hemoglobin. Tributyrin could also improve the performance of dairy cows. These results suggested that tributylglycerol may have a positive effect on breast health of dairy cows. In conclusion, these results indicated that tributyrin could relieve HS and increase the production performance of dairy cows by reducing the inflammatory responses of lymphocytes.

Levels and degradability of crude protein in digestive metabolism and performance of dairy cows

Two experiments were conducted to evaluate the effect of the level and degradability of crude protein (CP) on the digestive metabolism and productive performance of dairy cows. In both experiments, 15 Holstein cows with 585 ± 40 kg of body weight were distributed in a Latin square design with five contemporary squares, three periods of 21 days and three treatments. In experiment 1, treatments consisted of three CP levels (130, 160 or 180 g CP/kg DM), while in experiment 2, the treatments consisted of three levels of rumen degradable protein (RDP; 80, 100 or 120 g RDP/kg DM) in diets with average of 163 g CP/kg DM. Variables evaluated in both experiments were dry matter intake (DMI), total apparent digestibility, milk yield (MY) and composition, ruminal fermentation and N balance. In experiment 1, the increase of CP from 130 to 180 linearly increased the organic matter, CP, neutral detergent fiber (NDF) and acid detergent fiber (ADF) intake (kg) and the apparent total digestibility coefficient of DM and CP. In addition, a linear increase of MY, fat corrected milk (FCM) and daily production of fat, protein, lactose, casein and total solids was observed. A linear increase in ruminal ammoniacal nitrogen (NH3-N) concentration and nitrogen excretion in milk, feces and urine was also observed. However, there was no observed effect on SCFA concentration. In experiment 2, the increase of the RDP from 80 to 120 increased the DMI, MY, FCM, milk protein content and digestibility coefficient of the NDF, ADF and ethereal extract. Additionally, there was an increase in NH3-N concentration and milk nitrogen excretion. The studies indicated that the increase of CP content up to 100 g RDP/kg DM increased the DMI and the productive performance of the cows, but also increased urine N. Thus, it is desirable that the increase of the CP through the increase of the RDP is carried out up to 100 g of RDP/kg DM, since there is elimination of nitrogen, decrease of milk yield and decrease of propionic acid in values above that level.

Pasture characteristics and productive performance of dairy cows under two grazing management strategies

The objective of this work was to evaluate the supply, morphological and chemical composition of forage, performance and milk composition of crossbred cows managed in Megathyrsus maximus cv. Tanzania in intermittent grazing system with fixed and variable defoliation intervals according to the interception of 95% of photosynthetically active radiation by the canopy. The experimental design was a randomized complete block design with two treatments and two area replications. In each grazing cycle the supply, morphological composition, forage nutritive value, dry matter intake, milk production and composition were determined. The experimental area was 4.0 hectares, divided into two blocks of 2 hectares. Each block was subdivided into 22 pickets (11 for each treatment) totaling 44 pickets with an area of 909 m2 each. Significant differences (P &lt; 0.05) were observed for leaf blade content, in vitro digestibility of dry matter and forage crude protein, dry matter intake, individual and area milk production and lactose content milk, being the highest values found in the pastures managed with variable defoliation interval. Thus, the defoliation interval defined by the 95% interception of the photosynthetically luminosity leads to higher leaf blade proportions in the forage canopy, resulting in higher individual milk yield and per unit area, but with a loss of protein and total solids contents of milk.

Produksi Susu dan Lama Laktasi Sapi Perah Friesian Holstein di PT Ultra Peternakan Bandung Selatan

The aim of this research was to know the production performance of Holstein Friesian dairy cow on first lactation based on milk production records and length of lactation. The research was done at PT. Ultra Peternakan Bandung Selatan, West Java. A method of the research used descriptive analysis. Milk production and length lactation period on first lactation were used as production performance variable. The data obtained from 2011-2015 production record of 750 dairy cows on first lactation. Production performance of dairy cows on first lactation were have average daily milk production of 18,94 ± 3,74 Kg, 359,29 ± 81,14 days on length of lactation, 102,19 ± 65,27 days on peak production. The highest daily milk production of dairy cows in the first lactation period was 29,06 Kg, the smallest was 4,76 Kg, the longest length lactation was 872 days and the shortest was 195 days.

Timing of initiation and duration of feeding rumen-protected choline affects performance of lactating Holstein cows

Objectives were to evaluate the effects of altering timing of initiating and duration of supplementing rumen-protected choline (RPC) on lactation performance in dairy cows. The hypothesis was that RPC increases yields of milk and milk components, regardless of when supplementation is initiated, and that the effects of supplementing RPC starting prepartum and continuing post-transition would be additive. Cows at 241 ± 2.2 d of gestation were blocked by parity group (49 entering lactation 2, 50 entering lactation >2) and 305-d milk yield and, within block, assigned randomly to 1 of 4 treatments arranged as a 2 × 2 factorial with 2 levels of choline in transition, from 21 d pre- to 21 d postpartum, and 2 levels of choline in post-transition, from 22 to 105 d postpartum. The 2 levels of RPC supplemented were either 0 g/d or 12.9 g/d of choline ion fed as 60 g/d of an RPC product that was top-dressed onto the total mixed ration. Thus, treatments were as follows: NN (n = 25): no choline in transition or post-transition; NC (n = 25): no choline in transition and choline in post-transition; CN (n = 25): choline in transition and no choline in post-transition; CC (n = 24): choline in transition and in post-transition. Prepartum, treatments were supplemented (mean ± SD) for the last 18.8 ± 5.7 and 19.2 ± 5.0 d of gestation in treatments with 0 or 12.9 g/d of choline ion, respectively. Supplementing RPC prepartum did not affect dry matter intake (DMI), body weight (BW), or body condition score (BCS) in the last 3 weeks of gestation. Likewise, RPC did not affect the yield or the composition of colostrum. Supplementation with RPC during transition increased fat percent by 0.02 percentage units, fat yield by 0.16 kg/d, and energy-corrected milk (ECM) by 3.1 kg/d in the first 21 d postpartum, and increased fat yield by 0.10 kg/d and ECM by 2.4 kg/d from 22 to 105 d postpartum. Supplementing RPC during transition did not affect DMI postpartum, but it improved feed efficiency, and cows produced 0.11 kg/d more ECM per kg of DMI. Changes in BW and BCS during the first 21 d postpartum did not differ between treatments. Cows fed RPC during transition had more negative net energy balance and 0.1 unit smaller BCS in the first 105 d postpartum than non-supplemented cows. Supplementing RPC in post-transition did not influence productive performance in dairy cows, and choline supplementation during transition or post-transition did not affect measures of reproduction. Collectively, supplementing RPC to supply 12.9 g/d of choline ion benefited productive performance in dairy cows when supplementation occurred during the transition period, but no additional benefit was observed from supplementing RPC past 22 d postpartum.

Propionic acid bacteria enhance ruminal feed degradation and reduce methane production in vitro

ABSTRACT Thirty-one strains of propionic acid bacteria were screened for their effects on methane production and volatile fatty acid concentrations using in vitro assays of rumen fluid from Norwegian dairy cows and a grass silage–concentrate mixture as substrate. Nine of 31 strains were further analysed for effects on substrate degradation. Propionic acid bacteria led to reductions of up to 20% in methane production. Seven strains stimulated volatile fatty acid production, and in their presence in vitro substrate degradation tended to increase (P < .10). Most consistent results were found with Propionibacterium thoenii T159, which reduced methane production by 20% and caused 8% and 21% overall increases in substrate degradation and total volatile acid production, respectively (P < .05). Concomitant beneficial effects of a reduction in methane emissions and an increase in feed degradation suggest that this strain may be a promising tool for improving the productive performance of dairy cows.

Use of condensed molasses fermentation solubles as an alternative source of concentrates in dairy cows.

ObjectiveThe purpose of present study was to investigate the effects of condensed molasses fermentation solubles (CMS) on lactation performance, rumen fermentation, nutrient digestibility, and serum parameters of dairy cows.MethodsA total of 75 healthy Holstein cows with the same parity (milk production = 35±2.5 kg, body weight = 570±28 kg) were randomly selected and divided into 5 groups. One group served as control group (CON; no CMS), whereas the other 4 groups were CMS1 (accounted for 1% of the diet), CMS2 (2%), CMS3 (3%), and CMS4 (4%). All cows were fed regularly three times each day at 0800, 1600, and 2400 h. Cows received diet and water ad libitum. The experiment lasted for 60 days.ResultsResults showed that the dry matter intake, milk yield, and protein of CMS2 were maximum and higher (p<0.05) than CMS4. The ruminal pH was observed less than 6 in CMS3 and CMS4 groups. No noticeable difference of microbial protein was found between CON and CMS2 groups, while the microbial protein in these groups was higher (p<0.05) than CMS3 and CMS4 groups. The apparent digestibility of dry matter, organic matter, and crude protein in CMS2 group was higher (p<0.05) than CMS3 and CMS4 groups. Compared to CMS3 and CMS4 groups, the CMS2 group increased (p<0.05) the serum concentrations of immunoglobulin G and immunoglobulin M on d 60.ConclusionTherefore, it is practicable that CMS substitutes for a part of concentrates in lactating cows’ diets, but higher addition of CMS (more than 3% of the diet) could decrease production performance of dairy cows as seen in the present study.

Folic acid supplementation regulates milk production variables, metabolic associated genes and pathways in perinatal Holsteins.

Perinatal period is the critical time in dairy cattle due to negative energy balance and high milk production stress. Being a key role in biosynthesis and methylation cycle, folic acid is considered essential for lactational and metabolic performance in dairy cattle. Thus, the current study was designed to evaluate the effect of folic acid supplementation on milk production phenotypic traits in periparturient cows. Transcriptomic screening was performed for milk production and metabolism-associated differentially expressed genes. The 123 cows having similar parity, weight and expected date of calving were randomly selected and divided into three groups; A (n=41, folic acid 240mg/500kg cow/day), B (n=40, FA 120mg/500kg cow/day) and C (Control, n=42). Folic acid was supplemented for 21days (14days pre- and seven days post-calving), and three samples of blood lymphocytes were taken on day seven post-calving from each folic acid-treated and control group. In addition, the milk samples for each folic acid-treated group have been collected at 2nd, 3rd and 4th month of lactation. The increase in average milk yield noticed in group B were significantly (p-value<.05) higher than C and A. However, the data showed no noteworthy differences for milk fat and milk protein among the three groups. The transcriptomic analysis revealed that folic acid treatment regulated many key metabolic-related genes (DGAT2, ALOX5, LAP3, GPAT3, GGH, ALDOA, TKT) and pathways (glycolysis, folate biosynthesis, glutathione metabolism, etc.) in periparturient dairy cattle. It was concluded from the above findings that 120mg/500kg of folic acid quantity could be considered as a standard during the periparturient period to enhance the milk production performance of dairy cows. The transcriptomic profile revealed several metabolic and milk production-associated genes which could be a useful addition to the marker selection for the enhancement of metabolism and milk production of periparturient dairy cows.

Effect of using Acacia mearnsii tannin extract as a feed additive on nutritional variables and productive performance in dairy cows grazing a temperate pasture.

This study was carried out to evaluate the impact of including Acacia mearnsii tannin extract (TA) as a feed additive on nutrition and productive performance of dairy cows grazing a high-quality temperate pasture and receiving supplementation with a concentrate feedstuff. Fourteen multiparous Holstein cows were assigned to either of the following treatments: concentrate without or with 20g TA/kg dry matter (DM). Concentrate intake accounted for 32% of the total DM intake. Tannin addition increased the herbage DM intake by 22% (p<.05). There was no effect of TA inclusion on milk yield, milk composition, milk nitrogen (N) excretion, milk and plasma urea-N concentration, urinary excretion of total N, urea-N, and purine derivatives. However, TA inclusion increased the N intake and retention, total N excretion in manure, fecal N to urine N ratio, and decreased the dietary N efficiency for milk production and the percentage of ingested N excreted in urine (p<.05). In conclusion, supplementing dairy cows grazing a high-quality temperate pasture with a concentrate containing 20g TA/kg DM showed the potential of decreasing the proportion of ingested N excreted in urine without affecting the productive performance.

Influence of Natural Zeolites Supplemented with Inorganic Selenium on the Productive Performance of Dairy Cows

Influence of Natural Zeolites Supplemented with Inorganic Selenium on the Productive Performance of Dairy Cows

Effects of Partial Replacment of Dietary Forage Using Kelp Powder (Thallus laminariae) on Ruminal Fermentation and Lactation Performances of Dairy Cows.

Simple SummaryKelp powder has been widely used as feed ingredient or additives in monogastric animal production and presented positive effects on production performance and intestinal microbiota because of its abundance in biomass output, novel oligosaccharides, and iodine. However, little information is available for the nutritional effects of kelp on ruminants. Therefore, the objective of the present study was to investigate the effects of kelp partial replacing dietary forage on rumen fermentation and production performance of dairy cows. Results indicated that, a substitution of 5% kelp powder for forage in the diet reduced the fiber content in the feeding diet and then regulated the proliferation of ruminal microbiota, which lead to a significant reduction of NH3-N while increasing acetate, propionate, and total volatile fatty acids (TVFA) concentrations in rumen, and finally partly increasing milk production and milk fat content. The present study may provide more comprehensive cognition of the nutritional value of kelp powder as a dietary raw material. It may further promote the utilization of kelp powder and provide a useful dietary raw material to the husbandry of dairy cows.Background: Kelp powder, which was rich in novel oligosaccharides and iodine might be utilized by the rumen microbiome, promoted the ruminal fermentation and finally enhanced the lactation performance of dairy cows. Therefore, the purpose of this study was to investigate the effects of kelp powder partially replacing dietary forage on rumen fermentation and lactation performance of dairy cows. (2) Methods: In the present study, 20 Chinese Holstein dairy cows were randomly divided into two treatments, a control diet (CON) and a kelp powder replacing diet (Kelp) for a 35-d long trial. Dry matter intake (DMI), milk production, milk quality, ruminal fermentable parameters, and rumen microbiota were measured to investigate the effects of kelp powder feeding on dairy cows. (3) Results: On the lactation performance, kelp significantly increased milk iodine content and effectively enhanced milk production and milk fat content. On the fermentable aspects, kelp significantly raised TVFA while reducing the ammonia-N content. On the rumen microbial aspect, kelp feeding significantly promoted the proliferation of Firmicutes and Proteobacteria while suppressing Bacteroidetes. (4) Conclusion: kelp powder as an ingredient of feedstuff might promote the rumen fermentation ability and effectively increase milk fat and iodine content, and consequently improve the milk nutritional value.

Effects of feeding ground versus pelleted total mixed ration on digestion, rumen function and milk production performance of dairy cows

This study evaluated the effects of feeding ground (G) versus pelleted (P) total mixed ration (PTMR) on digestibility and milk production performance of mid‐lactation dairy cows over a 42‐days period. Results showed that, compared to GTMR, feeding PTMR increased dry matter intake and digestibility, ruminal propionate proportions and milk protein percentage, but decreased acetate‐to‐propionate ratio, milk fat percentage and aldehydes, acids, alcohols, and aromatic hydrocarbon proportions in milk. The PTMR did not improve milk yield but accelerated live weight recovery of cows and led to acidosis risk in lactating cows due to negative effects on rumen fermentation.

Meta-analysis of the effects of prepartum dietary protein on performance of dairy cows

The objectives were to review the published literature and use meta-analytic methods to determine the effects of dietary protein fed prepartum on productive performance of dairy cows. The hypothesis was that responses to dietary protein prepartum would differ between nulliparous and parous cows, and performance would be maximized at a greater protein supply in nulliparous than in parous cows. The literature was systematically reviewed, searching randomized experiments in which the prepartum dietary content or degradability of protein was manipulated. Twenty-seven experiments including 125 treatment means and 1,801 cows were included in the meta-analysis. Of those, 8 experiments with 27 treatment means reported responses for 510 nulliparous cows. Data collected included the ingredient composition and chemical analyses of prepartum diets, parity group, and means and respective measures of variance for productive responses. Mixed model meta-analysis was conducted and statistical models investigated the effects of dietary crude protein (CP) or supply of metabolizable protein (MP) prepartum on performance. Supply of MP was predicted using the National Research Council Nutrient Requirements of Dairy Cattle (2001) model. The mean ± standard deviation and median (range) concentrations of dietary CP fed to cows prepartum were 14.4 ± 2.2 and 14.4% (8.9 to 20.9%), resulting in mean and median (range) intakes of CP prepartum of 1,720 ± 432 and 1,734 g/d (745 to 2,482 g/d). Predicted prepartum supply of MP averaged 822 ± 157 in nulliparous cows, ranging from 517 to 1,094 g/d, and 1,146 ± 316 in parous cows, ranging from 463 to 1,733 g/d. Increasing prepartum CP content or predicted supply of MP improved postpartum dry matter intake in nulliparous cows, but increasing prepartum CP content reduced prepartum dry matter intake in parous cows. Yields of milk and fat-corrected milk increased with increasing prepartum supply of MP in nulliparous but not in parous cows. Yields of fat and protein increased in nulliparous cows with increased CP content or supply of MP. Alternatively, in parous cows, yield of milk fat was not influenced by supply of MP but responded quadratically to dietary CP content and was maximized at approximately 14% CP. Dietary CP had no effect on protein yield in parous cows, but increasing the supply of MP improved protein yield only in cows from experiments with >36 kg/d of milk production, whereas MP supply had no effect on protein yield of parous cows from experiments with <28 kg/d of milk production. Performance of dairy cows was responsive to prepartum supply of MP and nulliparous benefited from diets with increased MP intake. Based on current results, production responses for nulliparous cows increased linearly up to the maximum MP intake of 1,100 g/d observed in the study, whereas for parous cows, only yield of milk protein in cows producing more than 36 kg of milk/d was influenced by supplying more than 800 g/d of MP.

Peroxidase glutation effect in the health uterine and productive performance of dairy cows supplemented with selenium

Peroxidase glutation effect in the health uterine and productive performance of dairy cows supplemented with selenium

Effect of shortening close-up period length with or without lasalocid supplementation on production performance of dairy cows

To determine the effect of shortening the close-up period length with or without lasalocid supplementation on dry matter intake (DMI) as well as milk production and composition, 48 dry Holstein cows with 700±50 kg live body weight, 3-5 years old and BCS=3.75±0.25, in 210 days of pregnancy, were used. The experiment was planned as a completely randomized design with a 4 × 12 arrangement of treatments. Control group cows (21-L) had 21 days close-up dry period without lasalocid, second group (21+L) had 21 day close-up with lasalocid, third group (10-L) had shortened close-up dry period without lasalocid and last group (10+L) had shortened close-up period associated with lasalocid. The experiment started from inception of dry period and finished at the end of 42 days in milk (DIM). Results showed that although shortening of the period length associated with the use of lasalocid, could not alter prepartum DMI, but significantly increased postpartum DMI and milk production, improved feed conversion ratio (FCR), and decreased milk fat and protein content. Hence, shortening of close-up period from 21 days to 10 days associated with lasalocid supplementation resulted in better productivity of dairy cows.

Biochemical Profile and Productive Performance in Dairy Cows with Lameness During Postpartum Period

Background: Lameness in dairy cows is classified as a gait change caused by multifactorial process. The phase of the peripartum causes intense physiological changes for the adaptation of late gestation and onset of lactation. The aim of this study was to characterize the changes in the biochemical profile and productive performance in dairy cows with lameness during postpartum period.Materials, Methods &amp; Results: This study was conducted at the University of São Paulo farm, in Pirassununga, São Paulo State, Brazil, from January to March 2017. A total, of 48 multiparous (2 to 3 lactations and 3-4 years old) dairy cows, that had the milk production of 9,200 kg/ dairy cow in a period of 305 days in the previous lactation, were included in the study. All cows were managed under the same conditions and nutritional regimen. Evaluation of body condition score was performed by a single person on -18, -12, -8, -5, and -2 days before parturition, at parturition, and on days 1, 7, 14, 21, 30, 45 and 60 after parturition. Milk production was recorded on days 7, 14, 21, 30, 45 and 60 after parturition and saved in the software program. Blood samples were performed on -18, -12, -8, -5, and -2 days before parturition, at parturition, and on days 1, 7, 14, 21, 30, 45 and 60 after parturition. Blood samples were assayed for albumin, calcium, cholesterol, triglycerides, non-esterified fatty acids, β-hydroxybutyrate, urea, creatinine, gamma-glutamil-transferase and total protein concentrations. Dairy cows were divided into a lame group (11) and normal group (37) based on locomotion score from parturition to seven days postpartum. Lame cows was classified if their score was &gt; 2, and normal cows was classified if their score was ≤ 2 and free of any disease. Dairy cows that suffer by any health disorder other than lameness were excluded from this study. Cow diagnosed with lameness outside the diagnostic period were excluded from this study. The averages of the milk production, body condition score and biochemical profile were compared with the Tukey’s test. Lame cows showed lower (P &lt; 0.05) concentrations of albumin (on days -18 -12, -8, -5, -2 relative to parturition, at parturition, and on days 7, 14, 21, 45 and 60 after parturition) calcium (on days -18, -12, -8, -5 and -2 before parturition, and on days 7, 14, 21, 45 and 60 after parturition), cholesterol (on days -12, -8, -5 before parturition and on days 7, 14, 21, 30, 45 and 60 after parturition) than normal cows. Cow with lameness showed higher (P &lt; 0.05) concentration of triglyceride (on days 7, 14, 21, 30, 45 and 60 after parturition), non-esterified fatty acids (on days 1, 7, 14, 21, 30, 45 and 60 after parturition) and β-hydroxybutyrate (on days -12, -5 and -2 before parturition, at parturition, and on days 1, 14, 21 and 30 after parturition) than normal group. Cows with lameness presented higher (P &lt; 0.05) values of body condition score on days -18, -12 and -8 before parturition, and lower (P &lt; 0.05) values on days 7, 14, 21, 30, 45 and 60 after parturition than normal cows. Milk production was lower (P &lt; 0.05) for cows with lameness (on days 7, 14, 21, 30, 45 and 60 after parturition) than normal cows.Discussion: The overall prevalence of lameness in the evaluated period was 22.92% (11/48). Among lame cows, 07 presented laminitis, 02 had interdigital hyperplasia and 02 had sole ulcer. Our findings prove that the biochemical profile and productivity of dairy cows during the peripartum and postpartum period were affected by lameness at the early lactation.

Nitrogen utilization, whole-body urea-nitrogen kinetics, omasal nutrient flow, and production performance in dairy cows fed lactose as a partial replacement for barley starch

The objective of this study was to determine whether the partial replacement of barley starch with lactose (fed as dried whey permeate; DWP) affects N utilization, whole-body urea kinetics, and production in dairy cows. Eight lactating Holstein cows were used in a replicated 4 × 4 Latin square design with 28-d periods. Four cows in one Latin square were ruminally cannulated and used to determine dietary effects on whole-body urea kinetics and N utilization. Cows were fed a barley-based diet that contained 3.6% (dry matter basis) total sugar (TSG; designated control), or diets that contained 6.6, 9.6, or 12.6% TSG. Dietary TSG content was increased by the replacement of barley grain with DWP (83% lactose). Diets were isonitrogenous (∼17.3% crude protein), and starch contents of the control, 6.6, 9.6, and 12.6% TSG diets were 24.3, 22.2, 21.2, and 19.1%, respectively. Whole-body urea kinetics were measured using 4-d infusions of [15N15N]-urea with concurrent total collections of feces and urine. Dry matter intake (mean = 26.7 kg/d), milk yield (mean = 34.9 kg/d), and milk protein and fat contents were unaffected by diet. Ruminal ammonia-N concentration decreased linearly as TSG content increased, whereas ruminal butyrate concentration increased linearly as TSG content increased. Urinary excretion of total N and urea-N changed quadratically, whereas urinary excretion of total N (% of N intake) tended to change quadratically as TSG content increased. Fecal N excretion linearly increased as TSG content increased. A quadratic response was observed for total N excretion as TSG content increased. Milk N and retained N were not affected by diet. As TSG content increased, we observed quadratic responses in the omasal flow of fluid-associated and total bacterial nonammonia N, endogenous production of urea-N, urea-N recycled to the gastrointestinal tract, and urea-N returned to the ornithine cycle. Dietary TSG content did not affect the anabolic utilization of recycled urea-N or the proportion of recycled urea-N that was used for bacterial growth. Our results indicate that feeding DWP did not influence dry matter intake, milk yield, or milk composition. Feeding DWP decreased ruminal ammonia-N concentration, but this did not result in positive responses in milk protein secretion or N balance. The quadratic response in omasal flow of total bacterial nonammonia N indicated that including TSG beyond 9.6% of diet dry matter might depress ruminal microbial protein synthesis.

Effects of using Different Additives on Blood Metabolites, Digestibility of Nutrients and Production Performance of Dairy Cows

Effects of using Different Additives on Blood Metabolites, Digestibility of Nutrients and Production Performance of Dairy Cows