Growth Production Research Articles

In-Vessel Aerobic Composting of Organic Waste Using Synthetic and Natural Bulking Agents

The escalating growth in municipal solid waste production underscores the imperative need for efficient waste management, especially given that a substantial portion of MSW comprises biodegradable materials suitable for composting. This study investigates the impacts of using a synthetic bulking agent (perforated plastic balls) in conjunction with biochar, a natural bulking agent, on the aerobic composting of kitchen waste. The study aim to evaluate the effectiveness of this combined approach in optimizing the composting process. Four trials, each spanning 30 days, were conducted to assess the effects of different combinations of synthetic and natural bulking agents. The study uses a uniform-size perforated synthetic bulking agent in combination with biochar to enhance the composting process. Results indicate that incorporating the perforated plastic balls (8% by weight) alongside biochar significantly influenced key composting parameters. This approach accelerated the initial rise in temperature to 55°C, enhanced the reduction of total organic carbon, improved total Kjeldahl nitrogen retention, and facilitated the achievement of optimal carbon-nitrogen (C/N) ratios ranging from 19 to 20. Furthermore, the analysis of phosphorus (P) and potassium (K) loss during composting revealed that potassium is more susceptible to leaching than phosphorus due to its higher mobility. The study concludes that the addition of a uniform-size perforated synthetic bulking agent, constituting 8% (by weight), in conjunction with a natural bulking agent, effectively enhances the composting process.

СТАЛИЙ РОЗВИТОК ТОРГІВЛІ: ТЕОРЕТИЧНИЙ АСПЕКТ

The article highlights the theoretical aspect of sustainable trade development. It is found that trade development through more rational and efficient use of resources, production growth, increased income and investment leads to economic growth and employment. It is found that the sustainability of domestic trade is reflected primarily in its impact on human development. It is determined that the sustainable development of Ukraine's trade is laid down in the provisions of trade agreements between Ukraine and other countries and unions of countries. The author analyzes the features of the WTO agreement on sustainable trade development. The article analyzes the provisions of the EU-Ukraine Association Agreement in the area of trade policy development with regard to sustainable development. It is noted that trade is key to achieving the UN Sustainable Development Goals. It is found that the main direction of implementation of socio-economic management methods for sustainable trade development is performance management, which enhances the social orientation of management through the effect of activity and creates a system of motivation and incentives for labor. It has been found that trade is also important for social integration, which, along with the economy and the environment, is one of the three pillars of sustainable development. In Ukraine, environmental and sustainable development issues are poorly integrated into the process of formulating and implementing public policy, including trade policy. Therefore, environmental protection and sustainable development should be clearly enshrined in trade policy. The presence of environmental factors in a trade agreement cannot depend solely on the policies and approaches of the other party.

6619 An Unlikely Cause of Oligomenorrhea: Acromegaly Secondary to a Sparsely Granulated, Somatotroph-Lactotroph Adenoma

Abstract Disclosure: S.I. Alicea: None. Y. Wu: None. J. Marquez: None. C.S. Botero Suarez: None. M. Kinaan: None. Introduction: Acromegaly most commonly presents as a pituitary tumor that causes excessive production ofgrowth hormone (GH); and rarely due to excess of GHRH or ectopic production of GH. Approximately 15% of surgically removed pituitary adenomas represent GH-producing neoplasms, and about 5% are bihormonal, secreting both GH and prolactin (PRL). We present a case of an unexpected presentation of oligomenorrhea which ended up with the diagnosis of acromegaly secondary to plurihormonal mixed somatotroph-lactotroph sparsely granulated adenoma. Case Description A 32-year-old female with history of prediabetes was referred to our clinic by her gynecologistfor evaluation of oligomenorrhea which was initially attributed to polycystic ovarian syndrome.She reported menarche at age 10 and has experienced irregular menses and periods of amenorrhea over the years. Physical exam revealed macroglossia, a widened thickened nose, acanthosis nigricans in the neck skin folds, facial hair thickening, and significant thickening of all fingers, toes and soft tissues. Given physical exam findings, we evaluated the patient for acromegaly and lab testing revealed markedly high IGF I, mildly elevated prolactin, and hemoglobin A1C of 5.7%. An MRI of the brain with IV contrast revealed a heterogeneously enhancing pituitary macroadenoma measuring 2.9 x 2.9 x 2.2 cm with suprasellar extension resulting in mass effect on the optic chiasm without definite cavernous sinus extension. She was started on Cabergoline 0.5mg twice weekly temporarily and underwent pituitary transsphenoidal resection. Pathology results revealed tumor cells that were positive for Chromogranin and Cam5.2. About 10% of cells showed immunoreactivity for Prolactin and negative for TSH, ACTH, FSH, LH and immunostains. There was also weak focal (about 2%) immunoreactivity for GH. Occasional mitotic figures were present. The MIB-1 proliferation index was 3%. The reticulin stain demonstrates loss of acinar architecture, supporting the diagnosis. Aside from transient sodium fluctuations consistent with triphasic response, she had no long-lasting postoperative complications. One month after her surgery, her IGF I level and hemoglobin A1C are trending down and she reports symptomatic relief. She continues follow up in our clinic. Discussion This patient has acromegaly secondary to a mixed somatotroph-lactotroph sparsely granulated adenoma. Sparsely granulated adenomas are more common in younger populations, can be more aggressive, and are less responsive to somatostatin analogs. Early detection and treatment is imperative in the prevention of complications secondary to the disease. Our case will closely examine the differences in the clinical, prognostic, and pathologic features of sparsely- vs. densely granulated adenomas. We will also discuss the effects of acromegaly on gonadal function. Presentation: 6/1/2024

Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in Sidoarjo, Indonesia

The problem most often encountered in fish farming is disease which can cause a decrease in fish production levels. Parasites cause one of the infectious diseases found in fish farming. The presence of parasites can cause a decrease in the quality of the growth of fish production. The purpose of this study was to identify the prevalence of ectoparasites on Nile tilapia in Sidoarjo, Indonesia. Ninety-six samples from two ponds were examined under the microscope to find out the presence of ectoparasite that exists in the outside body part of the fish. Samples were collected from skin, fins, tail, and gills using native methods and observed under a microscope in the Division of Parasitology Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. The results of this study showed 86 samples positively infected by ectoparasite and 10 showed negative. The ectoparasites found in this study were Trichodina sp., Chilodonella sp., Ichthyophthirius multifillis, Gyrodactylus sp., and Dactylogyrus sp. The highest prevalence is Trichodina sp. and Dactylogyrus sp. The number of fish infested by ectoparasite is 89.56%, this is classified in the usual category. The highest prevalence occurs in infestations of Trichodina sp. i.e.72.91% which were contained in the 70 samples. While the lowest prevalence was in Gyrodactylus sp. by 8.32% in 8 fish samples. Double infections from two different ponds were 70% within 33 samples which were included in the usual category.

Isolation and Identification of Four Strains of Bacteria with Potential to Biodegrade Polyethylene and Polypropylene from Mangrove.

With the rapid growth of global plastic production, the degradation of microplastics (MPs) has received widespread attention, and the search for efficient biodegradation pathways has become a hot topic. The aim of this study was to screen mangrove sediment and surface water for bacteria capable of degrading polyethylene (PE) and polypropylene (PP) MPs. In this study, two strains of PE-degrading bacteria and two strains of PP-degrading candidate bacteria were obtained from mangrove, named Pseudomonas sp. strain GIA7, Bacillus cereus strain GIA17, Acinetobacter sp. strain GIB8, and Bacillus cereus strain GIB10. The results showed that the degradation rate of the bacteria increased gradually with the increase in degradation time for 60 days. Most of the MP-degrading bacteria had higher degradation rates in the presence of weak acid. The appropriate addition of Mg2+ and K+ was favorable to improve the degradation rate of MPs. Interestingly, high salt concentration inhibited the biodegradation of MPs. Results of scanning electron microscopy (SEM), atomic force microscopy (AFM), and Fourier-transform infrared spectroscopy (FTIR) indicated the degradation and surface changes of PP and PE MPs caused by candidate bacteria, which may depend on the biodegradation-related enzymes laccase and lipase. Our results indicated that these four bacterial strains may contribute to the biodegradation of MPs in the mangrove environment.

What is happening to the European Union aquaculture production? Investigating its stagnation and sustainability

While global aquaculture production has been highly successful in terms of production growth in recent decades, most developed countries including the European Union (EU) have not participated in this development to a significant extent. As recently as the 1970s, several EU countries were global leaders in aquaculture production, primarily focusing on filter feeders such as mussels and oysters. However, aquaculture production in the EU has largely stagnated over the past few decades. Despite political support, investment and research efforts over the last three decades, environmental regulations and competition with other activities have slowed down or even halted production growth in many cases. Recent EU legislation aimed at boosting food security and sustainable food systems, recognise aquaculture as a major potential contributor, focusing for example on sustainable or low environmental impact aquaculture, e.g. the production of low trophic level species. In this context, this article aims to contribute to the understanding of the EU aquaculture sector by examining the stagnation in production and its causes. We investigate the evolution of the EU aquaculture production in weight and value, as well as its average trophic level, length and diversity of the species produced. Results indicate an increase in the diversity of species produced, but a decline in the production of low trophic level species over the last two decades, and an increase in more challenging high trophic levels species, raising the overall average trophic level of the production. The decline in mussel and oyster production is mainly due to environmental and, to a lesser degree, economic factors. The rapid growth of carnivorous fish, such as seabream, seabass and salmon has slowed down since 2000, mainly due to regulations aiming at limiting environmental impacts.

Analyzing the Impact of Competitiveness Factors on Increasing Grain Production in Kazakhstan and Russia

The main research objective of this study is to conduct a detailed econometric analysis of the factors affecting the competitiveness of cereal production in Northern Kazakhstan and the Volga region of Russia, focusing on climatic conditions, irrigation systems, and soil cultivation technologies. The assessment of grain production competitiveness was considered for the period 2018-2020 in the context of the following indicators: grain yield, grain sown area, and gross grain harvest. The data analysis also included indicators of annual domestic grain consumption and total grain production in Kazakhstan and Russia. The study found that over the past few years, in the Volga region of Russia, wheat yields have been stably higher than those in Northern Kazakhstan, where the situation in grain production remains unstable. In the forthcoming decades, the production of grains is expected to exert a substantial influence on both regional and global food security. Russia’s grain production is the most important direction of national agriculture, which has the greatest potential for development in the near future. Kazakhstan, in turn, is poised to improve its competitiveness, given various opportunities to foster the growth of grain production. However, there is a need to modernize agricultural technologies and introduce climate-resistant agronomic systems.

Engineering Halomonas bluephagenesis for Synthesis of Polyhydroxybutyrate (PHB) in the Presence of High Nitrogen Containing Media

The trade-offs exist between microbial growth and bioproduct synthesis including intracellular polyester polyhydroxybutyrate (PHB). Under nitrogen limitation, more carbon flux is directed to PHB synthesis while growth is inhibited with diminishing overall carbon utilization, similar to the suboptimal carbon utilization during glycolysis-derived pyruvate decarboxylation. This study reconfigured the central carbon network of Halomonas bluphagenesis to improve PHB yield theoretically and practically. It was found that the downregulation of glutamine synthetase (GS) activity led to a synchronous improvement on PHB accumulation and cell growth under nitrogen non-limitation condition, increasing the PHB yield from glucose (g/g) to 85% of theoretical yield, PHB titer from 7.6 g/L to 12.9 g/L, and from 51 g/L to 65 g/L when grown in shake flasks containing a rich N-source, and grown in a fed-batch cultivation conducted in a 7-L bioreactor also containing a rich N-source, respectively. Results offer better metabolic balance between glucose conversion efficiency and microbial growth for economic PHB production.

Sous vide and chitooligosaccharide-catechin conjugate for inactivation of Vibrio parahaemolyticus and Shewanella algae in refrigerated Asian green mussel

Impacts of sous vide (SV) combined with chitooligosaccharide-catechin conjugate (COSC) on inactivation of pathogenic Vibrio parahaemolyticus and Shewanella algae in vitro and those inoculated in half-shelled Asian green mussel (H-AGM) were investigated. SV at varying temperatures (45 and 65 °C) and times (15, 30, 60, 90 and 120 min) was employed for treatment of H-AGM. SV process at 45 °C for 30 min rendered the yield of edible portion by 25.61%, maintained texture properties and inactivated vegetative microflora. COSC showed MIC/MBC toward V. parahaemolyticus and S. algae at 128/512 mg/L and 1,024/4,096 mg/L, respectively. For morphology, both bacterial cells had the pore and shrinkage when treated with COSC. In vitro study revealed that combining COSC (500 mg/L) with SV at 45 °C was optimal for inactivating V. parahaemolyticus and S. algae by >6 log10 CFU/mL within 30–90 min. However, the combination of COSC and SV at 45 °C for 30 min completely inactivated V. parahaemolyticus and S. algae inoculated in H-AGM during storage at 4 °C up to 9 days. Additionally, the virulence characteristics (tdh+ and β-hemolysis positive) of inoculated pathogenic V. parahaemolyticus were not detected during the 9–day storage period. Therefore, COSC along with mild SV process was effective in preserving the quality and inhibiting microbial growth in AGM products.

Architectural development of a land-attached carbonate platform in the African–Arabian Desert Belt: the late Pleistocene to Holocene evolution of the Al Wajh Platform, NE Red Sea, Saudi Arabia

ABSTRACT Carbonate platform architectures are indicators of environmental changes, such as sea level, climatic variations, and tectonic influence, which all control platform evolution. While analog studies on modern carbonate platforms have predominantly focused on tropical settings, limited attention has been given to arid and semiarid environments, although many ancient carbonate sequences were developed under these settings. This study aims to bridge this gap by investigating the Al Wajh carbonate platform lagoon in the NE Red Sea, Saudi Arabia, using geophysical and remote-sensing data to unravel its architecture and development since the last interglacial highstand (MIS 5e). We collected and analyzed sub-bottom profiles extending over 675 km and airborne lidar multibeam bathymetry data covering an area of 1700 km2. Surface sediment samples and vibracores with a maximum penetration of three meters were integrated to strengthen our interpretation. Furthermore, a recently published Red Sea sea-level curve was used to establish an age model and supplemented by available climate data to reconstruct depositional models. The Al Wajh lagoon is a “bucket” structure hosting five distinct depositional units since the late Pleistocene: U0 (pre-MIS 5e and MIS 5e), U1, U2, U3, and U4 (MIS 1), which are composed of five hydroacoustic facies, ranging from mounded to wavy laminated facies. Based on combining climate data, sea-level curves, and platform topography with our geophysical data, we conclude that the lagoon hosts carbonate, siliciclastic (fluvial and eolian), and potentially evaporitic deposits of open-marine and playa-lake origin. Intriguingly, karst architecture (i.e., sinkholes, caves, and collapsed cavities) is absent despite intermittent pluvial episodes and exposure during the late Pleistocene. Data analysis indicates that the Al Wajh lagoon architecture and development since the late Pleistocene are controlled by a combination of antecedent topography, sea-level variation, climate fluctuation, siliciclastic influx, carbonate production, and reef growth and drowning. Finally, insights from this study enhance our understanding of the architecture and sedimentary infill of ancient land-attached carbonate platforms deposited in a mature rift basin under alternating arid and pluvial climate settings.

Forecasting U.S. stock returns conditional on geopolitical risk and business cycles

Using standard predictors in the forecasting literature, we forecast the U.S. stock market returns conditional on geopolitical risk and business cycles over the 1927–2021 period. We find that out-of-sample forecasting performance is significantly better in times of high geopolitical risk versus low geopolitical risk. Consistent with previous research, we find further evidence of improved return predictability in recessions. However, we find little difference in forecasting performance in recessions versus expansions once the level of geopolitical risk is controlled for. We find similar results when using stock market cycles and periods of positive/negative industrial production growth in place of recessions/expansions. Our study contributes to the forecasting literature by documenting that geopolitical risk by itself and in combination with business cycle indicators impacts the forecasting ability of standard forecasting variables in the literature. We also contribute to the literature on the adaptive markets hypothesis with evidence of time-varying return predictability. We find inconclusive evidence as to whether our results are based on time-varying predictability or time-varying risk.

Migration processes in the Republic of Kazakhstan: current situation and consequences

Rural population migration is an objective process that affects the economic, environmental, social and demographic development of countries and their individual territories. Regulation and supervision of the migration process is one of the priority tasks of the state. The aim is to the scale and direction of migration flows in rural areas of the republic, the reasons for migration and the degree of influence on the labor market in rural areas are considered. Methods - comparisons to determine quantitative indicators characterizing the level of resettlement of economically active citizens. Assessment of the dynamics of those who arrived and left the RS was carried out by the methods of grouping and graphical analysis. Generalization method - in formulating the main aspects of resettlement. Results - the authors state that it is necessary to improve the mechanisms of legal labor migration, explanation and counseling, legal protection and organizational measures for those arriving and departing. Special attention should be paid to the development of infrastructure, creation of new jobs, career opportunities, high income, social conditions in rural settlements (RS) with high levels of local displacement. Based on the study, it is concluded that even positive trends and sustainable growth in production do not suppress the incentives for outflow to the city both for permanent residence and in search of temporary work. Conclusions - the location of rural areas near the regional center, the location of agricultural enterprises and farms, favorable natural and climatic conditions have a great impact on the attraction of migrants to rural areas. Based on the above, the key factor contributing to the reduction of migration loss of population can be called diversification of the economy in rural areas, further development of existing and creation of new production facilities.

Phycoremediation of Domestic Waste Water and Biodiesel Extraction from Fresh-Water Microalgae

ABSTRACT: Microalgae have recently drawn attention as a potential source for the sustainable production of biotechnologically valuable resources and improving the environment in various ways. They are widely distributed and can thrive even under extreme circumstances like high temperatures or high salinity. However, producing microalgae takes a lot of nutrients, which may have an adverse impact on the environment and the economy. The use of wastewater, particularly those from agro-industrial facilities, domestic waste waters, and industrial discharges, which often contain high nutrient concentrations, can serve as an alternative to synthetic culture media. Because the composition of wastewater and usual culture media is relatively similar, wastewater can be utilized to both clean itself and culture microalgae at the same time. Utilizing microalgae as wastewater bioremediation agents can efficiently remove N and P from domestic wastewater, maintain dissolved oxygen concentration, and reduce the various disease-causing pathogens and fecal bacteria that are present in domestic wastewater. The potential of microalgae to be used as a feedstock is increased by their ability to change the composition of their biomass under stress and accumulate lipids or carbohydrates that might be used to produce biodiesel. Methyl or ethyl esters of fatty acids produced from triglycerides by transesterification process by using renewable feedstocks are known as biodiesel. The microalgal biomass is considered as the next generation of feedstock for biofuel production. The Dual function of microalgae in domestic wastewater treatment and biomass growth for biodiesel production is outlined and discussed in detail in this review paper.

A Multi-Method Approach to Assess the Adoption of Precision Agriculture Technology in Brazil

Precision Agriculture (PA) application aims to increase crop productivity while minimizing environmental impacts. We analyzed the topics most studied in the advancement of crop production in Brazil by applying the concepts of PA using the systematic literature review (SLR). A multi-method approach combined an SLR applying the PRISMA method and secondary data analysis. We found five clusters of technologies using the PA concept related to hardware development and four clusters related to applying technologies to software development in the PA concept. Most topics focused on using sensors to control water (soil and environment), soil electrical conductivity, and data communication. The focus on sustainability led researchers to reduce chemical products related to fertilizers and pesticides using Variable Rate Fertilizers (VRT) and reducing the environmental loading. According to the research results, it was evident that PA technology might help farmers make more accurate decisions about cultivation, production, harvest, and soil management. The availability of decision support systems powered by big data and artificial intelligence to select the best crop for a given season and soil might assist Brazil's sustainable growth of food production.

Microbiological and Chemical Characteristics of Yoghurt Incorporated with Watermelon Rind Powder

Watermelon rind accounts for approximately one-third of the overall fruit mass. It is usually discarded due to its low commercial value. However, it is reported to contain valuable nutrients and is an effective source of pectin that can act as a potential prebiotic. This study aimed to study the effects of watermelon rind powder (WRP) on the growth of probiotic bacteria in yoghurt and its chemical characteristics. Watermelon rind was dried by using a dehydrator and ground into powder form before being incorporated into fresh yoghurt at 2% and 4% w/v. A sample with 0% w/v WRP was prepared as control. The effect of WRP on the growth of probiotic bacteria was determined by MRS plate count. Chemical analyses including titratable acidity, pH and Brix were conducted during the fermentation process. The results showed that the increase in WRP percentage resulted in a significant increase in bacterial growth with 7.20 ± 0.22 log CFU/mL for the control sample as compared to 8.42 ± 0.23 log CFU/mL for sample with 4% WRP after 30 hr of incubation. The fermentation time was also improved with the presence of WRP with a 0.22 h-1 increase in growth rate observed for the sample with 4% WRP as compared to the control sample. Furthermore, samples containing 4% WRP showed the highest increment in titratable acidity (12.47) and the highest percentage in Brix value reduction (51.04%) during the fermentation period as compared to the control sample. Biochemical analysis showed negative values for oxidase and catalase test while positive values were obtained for gram-staining indicating the presence of Lactic acid bacteria from the gram-positive group. This study demonstrates the high potential of WRP in promoting bacterial growth for yoghurt production which is beneficial to the food industry other than promoting the ongoing effort of food waste reduction.

Possibility of processing phosphogypsum to obtain an alkalizing reagent

Relevance. The need to develop a technology for the disposal of solid and liquid industrial waste to obtain organic-inorganic fertilizer. The growth of industrial production is accompanied by an increase in waste. In modern society, considerable attention is paid to the problem of processing man-made waste to obtain demanded products. Phosphogypsum is the main by-product of the production of fertilizers and phosphoric acid, it is a multi-tonnage waste, its storage leads to the withdrawal of vast territories from circulation. Liquid waste from pig farms also needs to be processed into organic fertilizers. Aim. To study the process of heat treatment of phosphogypsum in the presence of a reducing agent to obtain an alkalizing reagent. This will make it possible to further organize processing liquid and solid agricultural and industrial waste and produce domestic organic fertilizers Methods. Heat treatment of phosphogypsum in the presence of a reducing agent, followed by the preparation of an aqueous suspension to obtain an agent that reduces the acidity of livestock effluents. The samples of heat-treated phosphogypsum obtained during the study were characterized by X-ray phase analysis, electron microscopy. Suspensions with different pH values were obtained on their basis. Results and conclusions. The authors have studied heat treatment of large-tonnage inorganic waste of the chemical industry – phosphogypsum. It was found that the suspension of phosphogypsum heat-treated in the presence of a reducing agent has increased values of the hydrogen index, which can be used to obtain an alkalizing reagent for treating agricultural waste. The authors revealed the optimal technological modes of obtaining a reagent having the maximum pH value of the suspension: the amount of the introduced reducing agent is 0.16 mol/mol CaSO4, the heat treatment temperature is 1000 °C.

Из истории развития табаководства как важнейшей отрасли торгового земледелия в губер-ниях Южного Кавказа во второй половине XIX – начале XX века

The article focuses on the evolution of tobacco cultivation in the South Caucasus over the research period. The author investigates the spread of this technological culture throughout all provinces in the specified region. Information is provided on the quantity of land occupied for tobacco cultiva-tion, tobacco types, industrial firms and their owners, tobacco products produced, and tobacco sales locations. The author examines the many causes of the shift in demand for tobacco products over this historical time, as well as the unequal level of tobacco production. The essay discusses the excellent conditions for the growth of tobacco production in the South Caucasus, which eventually became commodities. The report also underlined the Russian government’s interest in spreading this crop, which was the primary raw material for the tobacco sector. The government, which contributed to the region’s colonial exploitation by the Russian and foreign bourgeoisie, benefited financially from the expansion of the cultivation of technologi-cal and precious crops. The author finds that the tobacco business in the South Caucasus evolved, improved, and gradually adopted the characteristics of commercial agriculture, which included commodity rela-tions. It is emphasized that the quick expansion of commercial agriculture is closely tied to the emergence of capitalist relationships in agriculture.

Analysis of the dynamics of production and foreign trade of fish products in Russia. Prospects for the development of the industry in the context of economic sanctions

Purpose: based on long-term data on the dynamics of fish catches and foreign trade in fish products, as well as international comparisons of world fishing powers, analyze the prospects for the development of the domestic fishing industry in the new economic conditions of sanctions confrontation with the countries of the collective West.Method: used such methods of general scientific knowledge as analysis and synthesis, as well as comparisons, tabular and graphical methods.Novelty: a detailed assessment of the factors affecting the growth of domestic fishing and fish farming, including the development of the domestic fishing fleet, was made. The prospects for expanding the sale of fish products on world markets, including on the basis of data on current and prospective consumption in different regions of the world, were considered.Results: the study analyzed a number of sources, including FAOSTAT and Rosstat commodity resource balances, in terms of production, consumption and cross-border trade in fish products. It has been established that after 2014 own consumption and imports are declining, while exports, on the contrary, are gradually increasing. Thus, the main driver of production growth in the industry is expansion to foreign markets. In the context of strengthening anti-Russian sanctions policy, it is necessary to diversify the sales markets for Russian fish products, however, an outdated production base is a significant limitation for the development of the industry.

The Impact of the Digital Economy on International Trade Models: Opportunities and Challenges

With the rapid development of the digital economy, international trade models are undergoing profound changes. Digital technology has reshaped traditional trade structures, driving the reorganization of global value chains and the rise of intangible assets. This paper explores the new characteristics of international trade in the context of the digital economy, including the transformation of trade structures driven by digitalization, the increasing importance of intangible assets in trade, and the influence of digital ecosystems. The digital economy has made trade processes more convenient and efficient, offering new globalization opportunities for emerging markets and small and medium-sized enterprises (SMEs), and promoting the growth of cross-border services and digital products. However, challenges remain, such as disparities in digital infrastructure, conflicts between digital governance and cross-border data flows, and the diversity of digital trade rules. This paper summarizes these opportunities and challenges and suggests future research directions to help policymakers, businesses, and technology providers navigate the complex landscape brought about by the digital economy.

In vitro evaluation of the clinical utility of Apitolisib/Vorinostat combination in Apitolisib-resistant H1975 lung adenocarcinoma cells

BackgroundThe PI3K signalling pathway regulates the metabolic activity of cells. Disruption by PI3K inhibitors causes an aerobic/anaerobic imbalance that decreases energy production and cell growth. Cancer cells adapt to PI3K inhibitors in order to reduce their effectiveness. Resistance to Apitolisib could be due to intrinsic factors or acquired adaptation. Oncologists often ask whether to discontinue Apitolisib, increase its dose, or use a drug combination.MethodsWe observed the proliferation of resistant cells in (H1975R+) and out (H1975R−) of Apitolisib treatment, cell cycle pattern, energy phenotyping/reprogramming, and the effects of combining Apitolisib with Vorinostat on the acquired proliferation of H1975R− cells.ResultsThe Proliferation of H1975R− cells increased, while that of H1975R+ cells remained suppressed. Both conditions showed a 5 × decrease in the number of cells at the Go/G1 phase and doubled at S and G2/M phases (p < 0.0001). Both H1975R− and H1975R+ cells exhibited decreased ECAR, with a stronger effect observed in H1975R+ cells (p < 0.0001). Oxygen consumption (OCR) increased significantly in H1975R− compared with that in H1975P (p = 0.02). The resistant cells became energetically active using mitochondrial respiration in drug-free medium; H1975R+ was hypo-energetic and consumed more free fatty acids (p = 0.0001). Ketone bodies in H1975R+ were increased by 40% and 2 × in BOHB and AcAc levels, respectively, compared to that in H1975P and H1975R− (p < 0.0001). H1975R− cell survival was 80% compared with 20% in H975R+ cells treated with 7 μM Vorinostat. Vorinostat effectively controlled acquired hyperproliferation of H1975R− cells.ConclusionIf a tumour becomes unresponsive to Apitolisib, it is advisable to continue the inhibitor and consider a combination with non-tyrosine kinase inhibitors.