Production Phase Research Articles

The Effect of Digital Analytics Capacity and Open Eco-Innovation on The Reduction And Waste Of Grain Losses

Objective: The study aims to analyze the effect of digital capacity and open process and management eco-innovation on the reduction and disposal of grain losses. Theoretical Framework: In this topic, digital analysis capacity and open eco-innovation are presented, providing a solid basis for understanding the context of the investigation. Method: The methodology adopted for this research comprises a quantitative approach, with grain companies in Brazil. Data was collected through a questionnaire, in which 175 responses were obtained and analyzed using structural equation modeling (PLS-SEM). Results/Discussion: The results obtained revealed that open-process eco-innovation helps in the prevention and disposal of grain losses. In contrast, open management eco-innovation helps in the disposal of grain losses. Furthermore, open process and management eco-innovation mediate the relationship between digital analysis capability and the allocation of grain losses. Research Implications: Practical and theoretical implications of this research are discussed, providing insights into how the results can be applied or influence practices in subject management and sustainability. Originality/Value: This study contributes to the literature on open process and management eco-innovation as an intermediary mechanism to explore the relationship between digital analysis capability and the prevention and allocation of grain losses. The relevance and value of this research are evidenced by showing managers strategies and ways to prevent grain losses that occur in the production, post-harvest, and processing phases.

Clean Hydrogen and Fuel Cell Vehicles

In this paper, a comprehensive synthesis of salient points from pertinent literature in the field is presented, accompanied by an analytical review of extant experimental data. The mechanisms underlying the utilization of green hydrogen in fuel cell vehicles are meticulously dissected from the perspectives of hydrogen production, storage, and utilization. An in-depth investigation into the energy conversion efficiency within the hydrogen production phase is conducted, as well as an examination of the energy conversion efficiency when utilizing green hydrogen as a fuel within fuel cell vehicles. Moreover, the article provides an informative overview of the fundamental attributes and performance metrics of fuel cell vehicles. It conducts a comparative analysis against traditional internal combustion engine vehicles, considering variables such as safety profiles, refueling duration, fuel mass, and fuel cost implications. The advantages of deploying fuel cell vehicles are analyzed, elucidating their benefits. Conversely, the article also enumerates the shortcomings and current bottlenecks faced by fuel cell vehicles, offering a critical view of their current limitations. Furthermore, the study delves into prevalent methods for green hydrogen production, positioning green hydrogen as a viable solution to the current challenges of carbon neutrality. The article culminates by suggesting the role of green hydrogen in spearheading future low-carbon development initiatives, thereby delineating a prospective roadmap for sustainable energy advancement.

Impact of Condensed Tannin and Sulfur Dioxide Addition on Acetaldehyde Accumulation and Anthocyanin Profile of Vitis vinifera L. Cv. Cabernet Sauvignon Wines During Alcoholic Fermentation

Acetaldehyde is a key carbonyl by-product during red wine alcoholic fermentation; it is reactive and takes part in certain reactions involving anthocyanin. The aim of this study was to investigate the influence of SO2 and condensed tannin on the acetaldehyde accumulation of Saccharomyces cerevisiae (S. cerevisiae) during alcoholic fermentation and the ripple effect on wine anthocyanin. In this study, six sets of Cabernet Sauvignon alcoholic fermentation with two different sulfur levels (HS and LS) were carried out by adding exogenous condensed tannins before fermentation (T0) in the acetaldehyde rise period (TA) of S. cerevisiae and at the end of fermentation (TE), separately. The acetaldehyde evolution was identified during fermentation and anthocyanin was analyzed comparatively. The results showed that HS treatment slowed down the degradation of acetaldehyde, while tannins accelerated the degradation of acetaldehyde during alcoholic fermentation, especially TA wines. Furthermore, TA wines possessed a unique anthocyanin profile after fermentation regardless of SO2 level compared with other wines. These results suggest that acetaldehyde-mediated anthocyanin polymerization most likely occurs timely at the acetaldehyde production phase of S. cerevisiae during alcoholic fermentation, and managing tannin addition time during production could be used to regulate the anthocyanin profile.

Trade-Off Between Growth Regimes in Chlorella vulgaris: Impact on Carotenoid Production

With the increasing awareness of socio-environmental issues, a global trend has emerged emphasizing the valorization of natural ingredients that promote health and well-being within sustainable production systems, such as microalgae-based carotenoids. Currently, little is understood about the correlation between biomass productivity and carotenoid content, which is a fundamental parameter for facilitating the immediate expansion of microalgae bioprocesses and ensuring the availability and industrial viability of these compounds. In this context, this study aims to investigate the carotenoid profile of Chlorella vulgaris through growth curve experiments conducted under photoautotrophic and heterotrophic regimes. Additionally, a trade-off analysis was performed for the production of carotenoids from microalgae. Carotenoids were quantified using high-performance liquid chromatography coupled with diode array and mass spectrometry detectors (HPLC-PDA-MS/MS). The performance of kinetic phases and energy demands across growth regimes was assessed to provide insights into production trade-offs. The results indicated that a total of 22 different carotenoids were identified in all the extracts. The all-trans-lutein and all-trans-β-carotene were the majority compounds. The total carotenoid content of Chlorella vulgaris revealed significant differences in the kinetic phases of carotenoid production, indicating that carotenoid volumetric production is only viable if the cultures are conducted until the log and stationary phases, based on the function of the biomass volumetric production (weight.volume−1). Therefore, the best trade-off for the process was to provide photoautotrophic growth until the exponential phase (log). Under this condition, the maximum carotenoid and lutein content was 2921.70 µg.L−1, reaching a maximum cell biomass of 1.46 g.L−1. From an environmental/economic point of view, the energy demand was 7.74 kWh.L−1. Finally, the scientific advances achieved in this study provide a holistic view of the influence of the main cultivation methods on the production of microalgae carotenoids, suggesting a viable initial direction for different industrial applications.

On the product phases and the reaction kinetics of carbothermic reduction of UO2+C at relatively low temperatures

The synthesis of UC using carbothermic reduction of UO2 and C mixtures has been well studied at high temperatures. However, the product phase behavior of carbothermic reduction at low temperatures (≤1773 K) is not well studied. Such a study is important as low temperatures permit single phase UC synthesis without forming secondary higher carbides, and it further supports the knowledge base of the process that needs to be used for transuranic elements such as plutonium that have high vapor pressures at elevated temperatures. Therefore, a low temperature carbothermic reduction of two different C/UO2 molar ratios under inert and reducing environments have been studied here. Two different sample holding crucibles, alumina (Al2O3) and graphite, were also used here to differentiate the hypostoichiometric (UC1-a) and oxygen dissolved (UC1-xOx) uranium monocarbide phases adding more details on the two systems. Also, the reaction kinetics involved in the formation of UC via the carbothermic reduction of UO2+C using product phases instead of evolved gases such as carbon monoxide is reported here. Under inert atmospheres but with significant oxygen partial pressures, the low temperature carbothermic reduction of UO2+C produced up to 90 wt.% UC1-xOx type oxycarbides as was confirmed by Xray powder diffraction. Reducing Ar-4%H2 environments at these temperatures were not successful in synthesizing UC as it reduces the amount of C required for the carbothermic reduction, leaving UC phase at a non-equilibrium state. Inert atmospheres with low or negligible oxygen partial pressures on the other hand produced near stoichiometric UC at high phase purity, especially at 1673 – 1773 K temperature range. An activation energy of 377±75 kJmol-1 was also calculated using product phase concentrations of the carbothermic reduction of UO2+C under these inert Ar(g) atmospheres.

Flyash and coffee silverskin binary blended geopolymer

Exploring alternative precursors other than flyash, slag, metakaolin and palm oil fuel ash for geopolymer synthesis has become very essential owing to the need for reduction in cost and accumulation of solid waste that could contribute to environmental pollution. The use of coffee silverskin (CSS) in binary blending with flyash (FA) in geopolymer synthesis is not yet fully understood, although its performance in ternary blending with silica fume for ordinary Portland cement (OPC) mortar has been established. Thus, binary blended CSS (0–30 %) with FA - such that CSS/(FA+CSS) varied from 0 to 0.3 - was activated by sodium hydroxide (8MNaOH) and sodium silicate (Na2SiO3), and then subsequently cured at 60 °C. Mechanical property, binder morphology, compound product phases and bond characteristics were studied through compressive strength test, scanning electron microscope (SEM/EDS), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIRS), respectively. CSS (CSS/(FA+CSS) = 0.3) significantly contributed to strength loss due to microcracks, carbon content and fiber induced microstructural discontinuity, such that 7-,14-and 28-d strengths recorded were 5.2, 7.8 and 9.7 MPa, respectively. Maximum 28-day strength of 16.5 MPa was recorded in CSS/(FA+CSS) = 0.1. CSS caused the formation of monetite, and disappearance of muscovite and augite in flyash/coffee silverskin based (FACSS) geopolymer.

Distribution of Salts in Milk and Cheese: Critical Methodological Aspects

The salt fractions of milk consist of cations (e.g., Ca, Mg, and Na) and anions (e.g., phosphate, citrate, and chloride). These salts are present as free ions or in complexes with other ions or proteins, primarily the caseins. Furthermore, significant levels of Ca and phosphate are also found in insoluble form, inside the casein micelles. The distribution of salts between this micellar phase and the soluble phase is important for the stability and properties of milk and dairy products. Various processes, such as (ultra-)centrifugation, (ultra-)filtration, dialysis, and selective precipitation have been used to separate the micellar and soluble phases in milk and dairy products to allow for studying the salts’ distribution between these phases. These different methods can lead to different levels of soluble salts because the salts in the supernatant from centrifugation, the permeate from ultrafiltration, and the diffusate from dialysis can differ notably. Hence, understanding which components are fractionated with these techniques and how this affects the levels of the soluble salts determined is critical for milk and dairy products. Applying the aforementioned methods to cheese products is further challenging because these methods are primarily developed for fractionating the soluble and micellar phases of milk. Instead, methods that analyze salts in water-soluble extracts, or soluble phases expressed from cheese by pressing or centrifugation are typically used. This review focuses on the significance of salt distribution and variations in salt fractions obtained using different methodologies for both milk and cheese.

A STUDY ON THE ENVIRONMENTAL IMPACTS OF CERTAIN BUILDING MATERIALS

The construction materials sector is a major industry. The cost of construction is influenced by the prices of the materials used. It is crucial to select building materials that are produced according to relevant standards and have passed quality control to minimize the environmental damage during their production, use, and disposal phases. Additionally, attention should be given to recycling and sustainability. In this context, the environmental impacts of certain construction materials have been examined in the study, and some recommended measures have been discussed.

Apoptotic Caspases Suppress Expression of Endogenous Retroviruses in HPV31+ Cells That Are Associated with Activation of an Innate Immune Response

Avoidance of an immune response is critical to completion of the human papillomavirus (HPV) life cycle, which occurs in the stratified epithelium and is linked to epithelial differentiation. We previously demonstrated that high-risk HPVs use apoptotic caspases to suppress an antiviral innate immune response during the productive phase of the life cycle. We found that caspase-8 and caspase-3 suppress a type I IFN-β and type III IFN-λ response by disabling the MDA5/MAVS double-stranded RNA (dsRNA) sensing pathway, indicating that immunogenic RNAs increase upon differentiation in HPV+ cells. In this study, we demonstrate that caspase inhibition results in aberrant transcription of a subset of endogenous retroviruses (ERVs) that have been shown to activate an IFN response through dsRNA-sensing pathways. We show that the increase in ERV transcription is accompanied by an enrichment in dsRNA formation. Additionally, we demonstrate that the robust increase in ERV expression requires activation of the JAK/STAT-signaling pathway, indicating that this subset of ERVs is IFN-inducible. Overall, these results suggest a model by which caspase activity blocks the reactivation of ERVs through the JAK/STAT pathway, protecting HPV+ cells from an increase in immunogenic dsRNAs that otherwise would trigger an IFN response that inhibits productive viral replication.

Traditional African Vegetables Knowledge Translation: A Scoping Review

Traditional African vegetables (TAVs) are an important component of an ecologically sustainable approach to addressing food and nutrition security in Africa. This review aims to identify the scope of the knowledge translation literature that exists across multiple aspects of the TAV value chain and to identify gaps in knowledge translation research. A scoping review following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) was conducted. The Population, Concept, and Context (PCC) framework was used to guide the development of inclusion and exclusion criteria for this review. The Web of Science, Scopus, Science Direct, and ABI/Inform databases were searched to identify the relevant studies which were then screened using the Covidence 2024 online software to select studies and extract information for analysis and reporting. A total of 182 studies/articles were selected and examined. The most significant knowledge documented about TAVs pertains to their vast genetic diversity contributing to their rich nutritional and medicinal properties, their ability to withstand harsh environmental conditions such as drought, pests, and diseases, as well as the effects of various post-handling techniques on the nutrient content of TAVs, with the production phase of the TAV value chain being the most studied. This review found that, although a breadth of knowledge about TAVs has been generated, there is still little documentation about transferring and utilizing this knowledge across the TAV value chain. This review identified that more research is needed to document the strategies and practices for the transfer and utilization of knowledge to ensure that it is utilized to enhance nutritional outcomes across Africa.

Coesistenze produttive. Abitare il paesaggio della Foresta di Petén in Guatemala

How does a community, living in an ecosystem complex like the tropical forest, develop new ways to have a productive relationship with the forest? The article presents the case study of the village of Uaxactún in Guatemala to show new ways of cohabiting the forest. The search for new alliances between man and forest also corresponds to new economies. The settlement, whose birth is closely linked to the productive capacity of the forest, allows us to start a broader reflection on the interaction between man and the biosphere, between the plurality of the anthropic and ecosystem subject. The new form of rurality presented coincides with collective management of the forest; at the same time, a specific Amerindian vision of nature and ways of making and being landscape emerges, through the various phases of production practice.The case study is investigated through fieldwork carried out in 2023 within the PhD in Landscape and Environment.

Decarbonizing Public Transportation: A Multi-Criteria Comparative Analysis of Battery Electric Buses and Fuel Cell Electric Buses

To combat global warming, many industrialized countries have announced plans to ban vehicles powered by fossil fuel in the near future. In alignment with this global initiative, many countries across the globe are committed to decarbonizing their public transportation sector, which significantly contributes to increased greenhouse gas emissions. A promising strategy to achieve this goal is the adoption of electric buses, specifically battery electric buses and fuel cell electric buses. Each technology offers distinct advantages and drawbacks, making the decision-making process complex. This research aims to answer two critical questions: What is the optimal choice for decarbonizing the bus transportation sector—electric battery buses or fuel cell electric buses? And what are the best energy carrier pathways for charging or refueling these buses? We propose a methodological framework based on multi-criteria decision-making to address these questions comprehensively. This framework utilizes the entropy weighting and the Technique for Order Preference by Similarity to the Ideal Solution (TOPSIS) methodologies to rank alternative bus technologies along with energy carrier pathways. The framework evaluates a range of criteria, including economic viability, energy demand, and environmental aspects. To illustrate the framework, we considered Qatar as a case study. Our results indicate that, with respect to economic viability and energy consumption, the operation of battery electric buses is favored over fuel cell electric buses, regardless of the energy pathway utilized during both the energy production and bus operation phases. However, from an environmental perspective, operating both bus alternatives using energy from green sources provides superior performance compared to when these buses are powered by natural gas sources.

Development of a Medical Device Lifecycle Research Database at the MFDS

Abstract So-called big data are potentially useful in the healthcare field and are of interest to researchers, industry, and government agencies. Over the past 10 years, Europe, the United States, and Asia have been making efforts to collect and systematically manage information on the entire process from clinical trials to follow-up management of medical devices (MD). We developed MD lifecycle research database at the Ministry of Food and Drug Safety (MFDS) of the Republic of Korea, consisting of 61 tables and 2,053 variables. The MFDS possesses statistical information on all MD, pharmaceuticals, foods, and human tissues produced and distributed. The information in the database is categorized according to four MD lifecycle phases-clinical trial, approval, production, and post-approval. For the clinical trial phase, the database contains information on planning and approval of clinical trial and non-clinical trial. The approval phase includes information on models, raw materials, quality, and changes, while the production phase stores information on production, import, export, supply and use. For the last step, follow-up management phase, provide information on post-marketing safety management, risk management, and administrative disposition. All four steps are linked based on primary key, so it is possible to understand information on the overall MD lifecycle. The MD Lifecycle Research Database will help strengthen the industry’s competitiveness in terms of cost-effectiveness in MD development and facilitate policy monitoring by improving its utilization in the public interest. This database contains information on all MD produced and distributed in Korea depending on the phases of the MD lifecycle, and data is easily accessible due to the lack risk of personal information exposure, so it can be widely used by individual researchers, industries, academia, and governments. Funding This research was supported by a grant (22063MFDS498) from ministry of food and drug safety in 2024. Key messages • We developed a medical device lifecycle database that enables tracking management according to the medical device lifecycle. • This database can be used as a scientific basis to ensure the safety, effectiveness, and efficiency of medical devices.

Evaluation of protease plus a precision-biotic on body weight gain and feed conversion ratio in broilers from 0 to 42 days of age

This study aims to evaluate the effects of a protease combination with a precision biotic on body weight gain (BWG) and feed conversion ratio (FCR) of broilers from day 0 to 42. 1,400 Cobb 500 male chicks were reared on seven different diets, including an unrestricted positive control (PC–Diet 1) and a negative control (NC–Diet 2) with slightly reduced crude protein, apparent metabolizable energy (AME), and digestible amino acids. Five additional treatment diets were formulated using the negative control and varying concentrations of a protease (Diets 3-4; 33ppm and 50ppm, respectively) and in combination with a precision biotic (Diets 5-7; 33ppm + 700ppm; 50ppm + 500ppm; and 50ppm + 700ppm, respectively). The PC treatment showed significantly different feed intake and FCR compared to all other treatments in all production phases (P < 0.05). There are no significant differences in non-PC treatments in all production phases for BWG and FCR (starter p=0.230 and 0.300; grower p=0.443 and 0.237; finisher p=0.450 and 0.896; overall p=0.111 and 0.497, respectively), nor is there any in terms of mortality (p=0.800). These findings may provide valuable information to producers to optimize flock performance efficiently and cost-effectively.

The Role of the Textile Industry and Permitting Systems in Advancing the Environmental Dimensions of the SDGs in the MENA Region

The textile industry plays a key role in driving development and human welfare, but it is also a sector struggling to address its contribution to the triple planetary crisis – climate change, biodiversity loss, and pollution. In the Middle East and North Africa (MENA) region, the environmental implications are compounded by the region’s water scarcity, land degradation and energy dependence. This article emphasises the urgent need to transition towards a sustainable and circular textile value chain to accelerate the achievement of the United Nations Sustainable Development Goals (SDGs). In this respect, permitting processes play a key role. However, the role of permitting systems in driving us towards a sustainable economy for textiles has not been exhaustively explored, if at all. Drawing upon research conducted by the UNEP Law Division in collaboration with Queensland University of Technology, as well as a broader literature review, this article examines the role of the textile industry in the MENA region and the importance of developing, or strengthening existing, legislation relating to permitting procedures, with a specific focus on Morocco, Türkiye, Jordan, and Egypt. The article underscores the pivotal role of environmental permitting processes in addressing the environmental footprint of the textile sector, mainly related to the production phase of the industry. While many countries already have permit processes in place, there is a need for policymakers to review and strengthen these frameworks to ensure comprehensive integration and address existing gaps. By doing so, countries can more effectively regulate and minimise the environmental impact of textile activities, thereby advancing the environmental dimensions of the SDGs.

3DP for sustainable built environment – Synthesis and thermomechanical characterization of composite materials based on local soil and date palm fiber waste

There is an increasing demand for sustainable construction materials to address the challenges posed by the environmental impact of the built environment (BE), which is driven by climate change, population growth, and urbanization. Conventional construction materials like cement contribute significantly to carbon emissions during production, transport, use, and disposal phases, and their poor thermal conductivity hinders efforts to maintain comfortable indoor environments. To address these challenges, there is an urgent need for innovative, locally sourced thermal insulation materials specifically designed for regions with extreme climates and high energy demands to maintain building comfort. This research explores synthesizing novel, environmentally friendly building materials using locally sourced date palm fiber waste and clay. The study also investigates the developed material's 3D printing (3DP) capabilities and optimizes its printing parameters with lab-scale prototypes. Additionally, thermo-mechanical characterization is conducted to assess its suitability for built environment applications. Different concentrations, from 1 to 5 wt% of date palm fiber to clay ratio (Dpf/C), were studied regarding microstructural, thermal, and mechanical properties, dimensional accuracy, and feasibility for 3DP of BE structures. Results demonstrated a significant reduction in thermal conductivity by 73%, achieving 0.244 W/mK, and an increase in compressive strength by 106%, reaching 10.9 MPa at 5 wt% Dpf/C. The promising thermomechanical properties of these composites and their suitability for 3DP support their use in real-world applications in the future's sustainable built environment. This scalable methodology can be adapted to regions with similar sustainable local and waste resources, advancing the circular economy.

The role of nitrogen and iron biogeochemical cycles in the production and export of dissolved organic matter in agricultural headwater catchments

Abstract. To better understand the seasonal variations in environmental conditions regulating dissolved organic matter (DOM) export in agricultural headwater catchments, we combined the monitoring of nitrate, iron, soluble phosphorus, and DOM concentration (as dissolved organic carbon; DOC) and composition (3D fluorescence) in soil and stream waters at regular intervals during 1 hydrological year. We installed 17 zero-tension lysimeters in organic-rich top soil horizons (15 cm below the surface) in the riparian area of a well-monitored agricultural catchment in French Brittany and collected them at a fortnightly frequency from October 2022 to June 2023. We observed a large increase in DOC concentrations in soil waters during the high-flow period linked to the establishment of Fe-reducing conditions and the subsequent release of DOM. We also noted that the timing and the spatial variability in Fe(II) biodissolution in soils was regulated by nitrate from agricultural origin and the heterogeneity of water flow paths at the hillslope scale. Contrary to our current understanding of DOM export in headwater catchments, these results lead us to consider the winter high-flow period as an active phase of both DOM production and export.

Experimental study on the products of coupling effect between microbial induced carbonate precipitation (MICP) and the pozzolanic effect of metakaolin

In this paper, the performance of the products resulting from of coupling effect between microbial induced carbonate precipitation (MICP) and the pozzolanic effect of metakaolin in the same system is analyzed and discussed in depth. The inoculation parameters of the bacterial solution were optimized, and a method of increasing pH of medium step by step was used to acclimate the alkali tolerance adaptation of Sporosarcina pasteurii. Mixed slurries (MC-M) were prepared and cured for 30 days and 180 days using a ratio of metakaolin (MK) to calcium hydroxide (CH) of 1:1, the bacterial solution as mixing water and calcium acetate as the source of introduced calcium. The compressive strength of the specimens was compared and the properties of the products cured for 180d were analyzed based on test methods such as nanoindentation, SEM, XRD, and TG-DSC. The test results show that the macroscopic mechanical properties of the MC-M specimens were effectively improved and the discrete degree of the micromechanical property parameters was reduced. The particle size distribution of the products increased, within 0.9–12 μm. The decomposition temperature of calcium carbonate in the products decreased, and the water loss temperature of the hydrated products increased. CaCO3 contains both aragonite and calcite phases, and the phase compositions of the hydrated products became more stable. This work demonstrates that MICP accelerates the depletion of CH in the pozzolanic reaction system and promotes the transformation of the hydration product phase composition. The pozzolanic effect reduces the particle size and degree of accumulation of calcite produced by MICP, resulting in a denser cured product. This phenomenon is referred to as the “coupling effect” of the two reactions and positively affects the mechanical properties, providing a new research idea on materials and methods for repairing concrete cracks.

Agronomic Evaluation and Yield Potentials of Grafting of Piper nigrum and Piper colobrinum

Foot rot disease (Phytophthora capsica) is Indonesia's primary challenge in cultivating pepper (Piper nigrum). Grafting to another Piper species, Piper coclobrinum, or melada (the local name), is a potential solution to increase resistance to this disease. The use of melada as rootstock has shown promising results during the seedling phase, but it has not been thoroughly evaluated during the production phase. The study was conducted in the pepper grower’s location in Puput and Simpang Katis Villages, Central Bangka, Bangka Belitung Islands Province. The study used kapok (Ceiba petandra) as the standard; plant morphology, leaf area, leaf thickness, and crown diameter for grafted melada and non-grafted pepper were recorded. Harvest variables included panicle length, panicle weight, the number of fruits per panicle, and fruit diameter, measured in three different areas of the canopy: the lower area (0-1 meter above the ground surface), the middle area (1-2 meters above the ground surface), and the top area (more than 2 meters above the ground surface). Physiological variables measured included rates of photosynthesis, stomatal conductance, transpiration rate, intercellular CO2, and CO2 conductance. The study demonstrated that the grafted pepper plants had a larger leaf area than the non-grafted pepper plants; the panicle weight and the number of fruits per panicle were higher. Panicles located in the middle zone of the plant canopy tended to be longer for grafted and non-grafted peppers. Panicle weight, fruit size, and the number of fruits per panicle were generally more significant at the top of the plant canopy. In all parts of the canopy, grafted peppers exhibited larger fruit size, greater panicle weight, and a higher number of fruits per panicle than non-grafted “Nyelungkup” peppers, highlighting the potential of melada as a rootstock for pepper plants.

Simulation-Based Optimization Workflow of CO2-EOR for Hydraulic Fractured Wells in Wolfcamp A Formation

Hydraulic fracturing has enabled production from unconventional reservoirs in the U.S., but production rates often decline sharply, limiting recovery factors to under 10%. This study proposes an optimization workflow for the CO2 huff-n-puff process for multistage-fractured horizontal wells in the Wolfcamp A formation in the Delaware Basin. The potential for enhanced oil recovery and CO2 sequestration simultaneously was addressed using a coupled geomechanics–reservoir simulation. Geomechanical properties were derived from a 1D mechanical earth model and integrated into reservoir simulation to replicate hydraulic fracture geometries. The fracture model was validated using a robust production history matching. A fluid phase behavior analysis refined the equation of state, and 1D slim tube simulations determined a minimum miscibility pressure of 4300 psi for CO2 injection. After the primary production phase, various CO2 injection rates were tested from 1 to 25 MMSCFD/well, resulting in incremental oil recovery ranging from 6.3% to 69.3%. Different injection, soaking and production cycles were analyzed to determine the ideal operating condition. The optimal scenario improved cumulative oil recovery by 68.8% while keeping the highest CO2 storage efficiency. The simulation approach proposed by this study provides a comprehensive and systematic workflow for evaluating and optimizing CO2 huff-n-puff in hydraulically fractured wells, enhancing the recovery factor of unconventional reservoirs.