Aims: To study the effect of Different Doses of Nitrogen and Seed Treatment with Biofertilizers on Growth, Yield and Seed Quality of Sunflower. Study Design: The experiment was laid out in a randomized block design with eleven treatments replicated thrice. Place and Duration of Study: The field experiment was conducted during rabi season of 2019-20, 2020-21, 2021-22 and 2022-2023 in the Instructional Farm of Ramkrishna Ashram Krishi Vigyan Kendra, Nimpith, South 24 Parganas, West Bengal. Methodology: The experiment was carried out in randomized block design with eleven treatments and replicated thrice. The treatments were Control, 50% N, 100% N (90 kg N/ha), Azospirillum (seed treatment), Azatobacter (seed treatment), Azospirillum +Azatobacter (seed treatment), 50% N + Azospirillum (seed treatment), 50% N + Azatobacter (seed treatment), 50% N +Azospirillum + Azatobacter (seed treatment), 75% N + Azospirillum + Azatobacter (seed treatment) and 100% N + Azospirillum + Azatobacter (seed treatment). A common dose of Nitrogen@ 90kg/ha, phosphorus @90kg/ha and potash @40kg/ha were applied. Results: Higher head diameter (13.82 cm), 1000 seed weight (65.50 g), seed yield (1830 kg/ha), oil yield (628.80 kg/ha), gross return (Rs.48581), net return (Rs.26368) and B:C (2.19) than all other treatment followed by T10 (75% N + Azospirillum + Azotobacter) and T3 (100% N) which were statistically at par with each other. Conclusion: On the basis of all parameter as well as economics it can be concluded that treatment T10 (75% N + Azospirillum + Azotobacter) can be recommended for better benefit and sustainable production of sunflower. The results of this experiment showed that use of chemical fertilizers can be reduced by using a combination of bio-fertilizers and inorganic nitrogen fertilizer.