Red Wine Production Research Articles

Sweet Red Wine Production: Effects of Fermentation Stages and Ultrasound Technology on Wine Characteristics

Three different sweet red wines were produced using Tempranillo grapes with three different sugar concentrations: 25, 50 and 75 g/L, using sulfur dioxide and low temperature to stop the alcoholic fermentation. They were compared to the wine produced without stopping the alcoholic fermentation. Cold pre-fermentation macerations and ultrasound during the post-fermentation stage were applied to try to improve the organoleptic properties specifically for sweet wines. The treatment with ultrasound after stopping the fermentation enhanced the extraction of anthocyanins compared to the wines produced without ultrasound, increasing the red color of the final wines, resulting in increments in the range of 16–30%. In any case, significant differences were found between the regular dry red wine and the sweet wines in terms of polyphenolic content, anthocyanin, tannin concentration and absorbance at 520 nm, with lower contents for the sweet wines. The wines were evaluated by different tasting panels showing different results depending on the tasting panel composition. First, an inverse relationship was observed between the sweetness of the wines and their acidity, bitterness, and astringency descriptors. Additionally, wines with higher sugar levels were described as having the most fruity aromas. Finally, no differences in vegetal aromas were found in the different wines.

Evaluation of protein-polysaccharide interactions through ζ-potential and particle size measurements to assess their functionality in wine.

Protein-polysaccharide-tannin interactions are important in every aspect of red wine production from physical stability to color, astringency, and body. For this model study, bovine serum albumin (BSA) was selected as the protein, while carboxymethyl cellulose (CMC), mannoproteins, and pectin were the model polysaccharides. Each protein-polysaccharide combination was analyzed for zeta (ζ) potential and particle size at neutral pH and within the wine-like solution. Mixtures were assessed regarding their protective, affinitive, and aggregative behaviors. Based on their individual ζ-potentials, pectin and mannoprotein were most stable at lower concentrations. At higher concentrations, they reduced the suspension's stability and increased the aggregate sizes. CMC consistently increased the stability of any solution under neutral pH conditions. However, with increasing concentrations, these large aggregates are expected to precipitate. Fruit pectin (FP) and BSA interactions seemed to be the main factors in the formation of visible precipitates at neutral pH. FP and the mannoprotein decreased stability enough to cause precipitation without haze formation. The mannoprotein decreased particle sizes, in both the suspension and precipitation, which may indicate greater selectivity toward proteins. FP also decreased the suspended particle sizes under wine conditions. These findings demonstrate the use of ζ-potential and particle size values to characterize macromolecular interactions in model systems and can also be used to indicate effective fining agents. PRACTICAL APPLICATION: This work demonstrates the capabilities of ζ-potential analysis paired with size particle measurements to predict and characterize the interactions between macromolecules in complex systems. The interactions between model wine macromolecules can be evaluated with this technology at a level that cannot be reached with any other analytical technique.

Challenges to Viticulture in Montenegro under Climate Change

The Montenegrin climate is characterised as very heterogeneous due to its complex topography. The viticultural heritage, dating back to before the Roman empire, is settled in a Mediterranean climate region, located south of the capital Podgorica, where climate conditions favour red wine production. However, an overall increase in warmer and drier periods affects traditional viticulture. The present study aims to discuss climate change impacts on Montenegrin viticulture. Bioclimatic indices, ensembled from five climate models, were analysed for both historical (1981–2010) and future (2041–2070) periods upon three socio-economic pathways: SSP1-2.6, SSP3-7.0 and SSP5-8.5. CHELSA (≈1 km) was the selected dataset for this analysis. Obtained results for all scenarios have shown the suppression of baseline conditions for viticulture. The average summer temperature might reach around 29.5 °C, and the growing season average temperature could become higher than 23.5 °C, advancing phenological events. The Winkler index is estimated to range from 2900 °C up to 3100 °C, which is too hot for viticulture. Montenegrin viticulture requires the application of adaptation measures focused on reducing temperature-increase impacts. The implementation of adaptation measures shall start in the coming years, to assure the lasting productivity and sustainability of viticulture.

Identifying Hotspots and Most Relevant Flows for Red and White Wine Production in Brazil through Life Cycle Assessment: A Case Study

This study undertakes a Life Cycle Assessment (LCA) of red and white wine production in Brazil, focusing on a leading winery in the Serra Gaúcha region. Given the notable lack of similar research in this area, our study employs LCA methodologies to evaluate the environmental impacts associated with the winery stage. Our results indicate that although white wine generally exhibits a lower environmental impact than red wine, the specific impacts differ substantially across various environmental categories, highlighting the necessity for context-specific assessments. We also find that electricity consumption, sugar usage, and liquid CO2 production are the primary drivers of environmental impacts, especially in toxicity-related categories. Moreover, our research critically examines the suitability of the Product Environmental Footprint Category Rules (PEFCR) within the Brazilian context and proposes a revised set of impact categories. This revision enhances the accounted impacts from 55% in the existing PEFCR 2.0 to 80% in our recommended framework, suggesting the need for more tailored environmental impact assessment tools in regional settings. Through these findings, our study not only addresses a significant gap in LCA research within the Brazilian wine industry but also sets the stage for improved environmental management practices across Brazilian wine industries. These enhancements are crucial for aligning local production processes with global sustainability standards and advancing the environmental stewardship of the wine sector in Brazil.

THE IMPACT OF CLIMATE CHANGE ON THE SUSTAINABILITY OF WINE PRODUCTION AND THE STRUCTURE OF WINE CONSUMPTION IN THE NORTHEAST REGION OF ROMANIA

This study was carried out with the aim of investigating and analysing different aspects related to viticulture and wine production in the northeast region of Romania. The methodology focused on the use of statistical data and field research, with an emphasis on the impact of potential climate change on wine production and consumer preferences. The analysis of the evolution of vine cultivation in the northeast region of Romania was carried out using statistical data on grape harvest, area planted to vines and wine production. The results showed significant changes in these indicators over the period analysed (2013-2022), reflecting the dynamics of the wine industry in the region. A survey of a sample of 50 respondents provided relevant data on their preferences. Analysis of this data revealed information on preferred wine types, consumption habits and purchasing preferences. Although the population‘s preference for white wine is evident, climate change may pose a challenge for the region, and in the future, it may be necessary to adapt vineyard locations and cultivate vine varieties more suitable for red wine production. These issues have important implications for planning and developing the future of the wine industry in the northeast region.

A Biodiversity Monitoring Case Study in Viticulture: Manual and Digitalized Collaborative Methodology to Pursue the European Commission’s Sustainable Challenges

The European Commission (EC) promotes policies addressing the needs of a growing human population while adhering to ecological principles that ensure ecosystem sustainability. Viticulture, one of the most widespread cultivations in the world, is, at the same time, one of the most environmentally impactful cultivations. Many studies have been conducted worldwide to identify more sustainable practices because vine monoculture, when repeated for long periods in the same territory, combined with low attention to the agronomic balance of vineyards, is causing biodiversity loss. The study aims to implement analytics for the accounting of biodiversity supported by digital tools like smart applications and digital platforms. Two farms were analyzed in a vacated area for red wine production in the center of Tuscany (Italy). A conventional biodiversity assessment protocol was used to evaluate the magnitude of biodiversity. Smartphone applications and a digital database creation platform supported this. The results highlighted an overall low level of biodiversity from a biodiversity perspective, while the use of smart applications and digital platforms represents an efficient tool for mitigating recognition errors in flora and fauna assessments and a powerful instrument for monitoring and tracking farm biodiversity. The study provides an overview of biodiversity status in a wine production area and a methodology to make its assessment easier and more reliable.

Phytochemical and Pharmacological Review of the Genus Antidesma

Background Antidesma is a large genus consisting of around 100 species, which are widely distributed in the tropics and subtropics of Asia, some species can be found in Africa, the Pacific islands, and Australia. The uses of Antidesma plants range from food (for juice, jam, and red wine production) to medicinal purposes (for treatment of indigestion, pneumonia, menstrual regulation, and osteoarthritis). Methods The review was conducted by gathering relevant literature from secondary databases, including Web of Science, Google Scholar, and PubMed, as well as specialized books and websites. Results There have been more than 140 compounds reported as chemical constituents of the genus Antidesma, which mainly belonged to alkaloid, phenolic, flavonoid, lignan, and terpenoid classes. The high content of phenolic compounds was found in A. bunius and A. thwaitesianum fruits, which also correlates with the potential anti-oxidant activities of respective plants. Besides that, the antibiotic, antifungal, and anti-inflammatory activities, as well as effects on metabolism were also investigated and reported. Conclusion The plants of the Antidesma genus have a wide range of applications in food and medicine. The published results have shown that the genus is a precious source of bioactive natural compounds, which was shown beneficial for preventive medicine. Further research is required to promote the application in the healthcare system.

Co-inoculation of Meyerozyma guilliermondii and Saccharomyces cerevisiae reduced alcohol content and improved flavor profiles of red pitaya (Hylocereus polyrhizus) wine

Abstract Red pitaya (Hylocereus polyrhizus) is cultivated in tropical and subtropical regions worldwide. Red pitaya is rich in nutritional and medicinal ingredients. Oenological yeasts play vital roles in wine fermentation by metabolizing a variety of aromatic compounds that affect the flavor and sensory properties of wine. In this study, we examined the influence of Meyerozyma guilliermondii on the physicochemical parameters, sensory properties, and aroma chemical composition of red pitaya wine co-inoculated with Saccharomyces cerevisiae. The results showed that mixed inoculation of M. guilliermondii and S. cerevisiae reduced the alcohol content of red pitaya wine. Moreover, using M. guilliermondii and S. cerevisiae as fermentation starters increased the content of volatile alcohols, aldehydes, ketones, and other compounds, as well as the types of alcohols, aldehydes, and ketones. In addition, M. guilliermondii did not affect the sensory characteristics of the red pitaya wine. Therefore, co-inoculation of M. guilliermondii and S. cerevisiae was helpful in enhancing the richness and complexity and improving the aromatic flavor properties of red pitaya wine. The results obtained in this study could serve as a reference for the production of red pitaya wine and other fruit wines.

Influence of different baking temperatures of red kojic rice on the physicochemical properties, antioxidant capacity, and functional components of red starter wine.

To improve the quality of red starter wine, this study explored the effects of baking red kojic rice at varying temperatures on the physicochemical characteristics of red starter wine. Baking was predicated on understanding crucial enzyme activities and starch granule structure of red kojic rice at 75, 95, and 105 °C, leading to the production of three red starter wine variants (BHQW1, BHQW2, and BHQW3). The results revealed an increased alcohol (increase 0.50%), total sugar (increase 0.14 g L-1 ), and total acid (increase 0.54 g L-1 ) content in red starter wine fermented using baked red kojic rice compared with the control group (wine fermented with unbaked rice, HQW). Furthermore, both the 105 °C baked red kojic rice and its resulting BHQW3 demonstrated significantly higher red color values than HQW (increase 2.03 U g-1 and 0.15 U mL-1 respectively). The highest lovastatin content was presented in red kojic rice baked at 105 °C and its corresponding fermented wine (1420.63 ± 507.9 μg g-1 and 3368.87 ± 228.16 μg L-1 respectively). Additionally, BHQW groups displayed higher total flavonoids and phenols content than HQW. Regarding antioxidant capacity, all BHQW groups showed stronger overall antioxidant capacity than HQW. The determination of volatile components revealed the highest content of volatile compounds in BHQW2 (2621.19 ± 548.24 μg L-1 ) and significantly higher volatile esters in BHQW1 (254.46 ± 16.63 μg L-1 ). Moreover, 16 volatile compounds were identified only in BHQW groups, including isoamyl caprylate, 2-ethylhexyl alcohol, and benzaldehyde. Our findings suggested that the baking technique of red kojic rice could enhance the quality of red starter wine through enhancing antioxidant properties, increasing functional components, and enriching volatile flavor compounds, thus providing a foundation for new techniques in red starter wine production. © 2023 Society of Chemical Industry.

INFLUENCE OF THE ROOTSTOCK P1103 AND SO4 ON THE BIOLOGICAL CHARACTERISTICS OF THE CABERNET SAUVIGNON VARIETY

Cabernet Sauvignon is one of the most widespread grape varieties, both in the world and in Serbia and is used for the production of high quality red wines. The rootstocks P 1103 and SO4 are widely used in Serbian viticulture. The choice of rootstock can significantly influence the biological characteristics of cultivated variety, especially through qualitative parameters of bunch and the berry. The research was conducted in the wine-growing region of Šumadija. The vineyard was planted in 2009 with a row spacing of 2.3 m and between rows 0.8 m. The aim of the work was to determine the influence of rootstock P1103 and SO4 on the biological characteristics of the Cabernet Sauvignon variety. The work contains data related to phenological observations, vegetative and reproductive potential, yield, uvological composition of grapes and berries, and qualitative characteristics of grape juice. Phenological observations revealed no differences in the length of phenophases. A higher average shoot growth was observed on rootstock P 1103 (150.9 cm). A higher number of developed inflorescences (13.7) was observed on rootstock SO4, while the average yield was higher on rootstock P 1103 (1.723 kg). The uvological observations of bunches and berries showed higher values on the rootstock P 1103 for the following parameters: average weight of the bunch (175.4 g), number of berries in the bunch (151.6), weight of the bunch stem (5.5 g), weight of skin of 100 berries (11.9 g) and seed mass of 100 berries (7.2 g). The sugar content of grape juice was higher in rootstock P 1103 (27.16%), while the content of total acids was generally higher in rootstock SO4 (8.3 g/l).

Reshaping yeast metabolism and enhancing the quality of fresh-style red wine through low-temperature fermentation

Fresh-style red wine is attracting consumers, prompting an exploration of low-temperature winemaking techniques for its industrial production. Herein, low temperatures were applied to red winemaking, evaluating metabolite compositions and sensory profiles of wines fermented at lower versus higher temperatures. The accumulation of various phenolics at different temperatures may work synergistically with temperatures to shape yeast metabolism. Low temperatures significantly weakened the extraction of phenolic compounds, thus attenuating the bitterness and astringency of wine and contributing better balance and brighter color to wines at lower temperatures. Esters, especially ethyl acetate (increased by 24.8%), ethyl hexanoate (increased by 72.3%), ethyl octanoate (increased by 67.9%), and ethyl decanoate (increased by 50.6%) increased with decreasing temperatures, contributing fresh red fruit aromas to wines fermented at 10 °C, while phenylethanol (decreased by 60.0%) decreased with decreasing temperatures, weakening floral aromas to wines at 10 °C. Low temperatures can reduce higher alcohols (19.3% in total) and positively influence the sensory quality of wine. In general, wines fermented at 10 °C exhibited the highest overall quality, highlighting the potential of low-temperature fermentation in the production of fresh-style red wine.

INNOVATIVE DESIGN TECHNOLOGIES OF A MODERN MINIWINERY TAKING INTO AC-COUNT THE TERROIR FOR PINOT NOIR

The article presents a brief overview of scientific works that were aimed at studying the influence of terroir, harvest and technology on the quality and specificity of wine. Modern scientific methods for identifying wines from different regions are considered. Modern studies by Chinese oenologists have shown that a number of trace elements in wine (Cu, Zn and Mn) can be an indicator of the region of origin of the wine. Scientists from Australia, New Zealand, the USA, France and Portugal have also obtained interesting results in the identification of wines of different vintages and terroirs. Scientists have shown that the originality of table wines of specific regions and subregions can be determined on the basis of modern highly sensitive methods of analysis (spectroscopic methods for studying compounds using the phenomenon of nuclear magnetic resonance; combined methods with the joint use of NMR and the study of the target differential sensor matrix; fluorescent methods for determining the content of amino acids and amines, methods of cluster analysis, etc.). When determining the terroir for red wines, many researchers in different regions of our planet paid special attention to the Pinot Noir variety. This is due to the fact that for this particular variety, terroir plays a huge role in shaping the style of the future wine. At the Vinoman winery, together with the Odessa National Technological University, a comparative analysis of red table wines Pinot Noir from three terroirs of the southern part of the Odessa region was carried out. In addition, as part of the master's qualification work, an innovative project for the reconstruction of an existing enterprise was proposed, which involves the implementation of the gravitational principle of winemaking. The project takes into account the features of the area, climatic conditions and the range of products. Particular attention was paid to the implementation of all of the above aspects in the technology for the production of high-quality red table wines Pinot Noir. As a result of the work carried out, physical and chemical indicators were studied and a sensory analysis of the wines of the Bolgrad, Izmail and Reni terroirs was carried out. Based on the data obtained, preliminary conclusions were made about the prospects for the production of red table wines Pinot Noir at the Vinoman enterprise from certain terroirs in the south of the Odessa region.

ИССЛЕДОВАНИЕ СКЛОННОСТИ ФЕНОЛЬНЫХ ВЕЩЕСТВ ВИНОМАТЕРИАЛОВ И ВИН ИЗ ВИНОГРАДА СОРТА КАБЕРНЕ СОВИНЬОН К ОКИСЛЕНИЮ

One of the most important tasks in the production of high-quality red wines is the preservation of intense color, which exposed to atmospheric oxygen, can change and acquire garnet or brown shades. The intensity of color, its shades and stability are determined by the presence of phenolic compounds in wine, among which polymeric forms of polyphenols and anthocyanins have a rich color. In addition, the phenolic substances of red wines are represented by monomeric forms, catechins and procyanidins, etc. The impact of oxygen at various stages of the technological process of making wine can have both positive and negative effects on its chemical composition and organoleptic characteristics. The purpose of this research was to study the effect of oxygen introduced at various technological stages of the preparation of wine materials. As a result of studies of the indicators of the mass concentration of oxygen and phenolic substances that cause wine oxidation, as well as the organoleptic indicators of dry red wines (production samples) made from the Cabernet Sauvignon grape variety in various geographical zones of southern Russia, significant differences have been revealed due to agro-climatic conditions of growing grapes in a particular area, as well as the grape processing technology used at enterprises. Under the conditions of the laboratory and production unit «Micro wine-making», the possibility of using the microoxidation method both at the stage of must and pulp fermentation, and after the fermentation process (stabilization and clarification) for the purpose of deliberate oxidation of polyphenols, was shown. Based on the conducted studies, the optimal dosage of exogenous oxygen, which has a positive effect on the organoleptic characteristics of wine materials, is a concentration of 4-6 mg/dm3 both during microoxidation at the stage of must and pulp fermentation, and at the end of the fermentation of wine.

Microbiological and biotechnological study of yeast strains isolated during spontaneous fermentation of black grape variety ”Codrinschii”

An important task in the field of wine microbiology is obtaining local yeast strains that can easily adapt to the specific environmental conditions, ferment the carbohydrates in the must, and produce natural wines with high organoleptic qualities. This work presents the results of microbiological research related to the isolation and selection of local yeast strains for the production of dry red wines. The isolated yeast strains were tested to identify those that could be used to produce wines with typical characteristics. Based on the assessment of biochemical and technological indices of 15 yeast strains isolated from the must of the "Codrinschii" variety, 9 yeast strains were selected for the production of dry red wines.

Vitis vinifera L. variety Syrah sprayed with ZnSO4: Effect on fruit quality and winemaking

In grapevines, Zn is essential for normal leaf growth, shoot elongation and pollen development, allowing a fully developed berry. In this context, using as a test system Vitis vinifera L. variety Syrah, this study aimed to assess the interactions between Zn enrichment in grapes and sugars and fatty acids profiles, further considering the sensory implications of red wine production. Vineyard conditions of the soil were assessed to ensure the natural optimal development of grapevines and the workflow for Zn enrichment considered three treatments: foliar spray with water (control) and with ZnSO4 (at 450 and 900g.ha-1). After the 2nd foliar application of ZnSO4, only minor changes of Zn, Ca and P contents were found in grapes (the levels of K, Cu and S increased significantly with ZnSO4 (450 g.ha-1). At harvest, the grapes submitted to foliar application of ZnSO4 showed significantly higher levels of Zn (between 33.38 - 54.41%), but significant deviations in sugars (sucrose, glucose and fructose), fatty acids (C18:0, C18:1, C18:2, C18:3, C16:0, C<16:0) and color parameters were not found. After winemaking, relative to the control, a higher content of Zn persisted (60.59 - 63.82%), without impairing the characteristics desired by consumers. In fact, the wine with ZnSO4 (900 g.ha-1) was the most sensorially accepted.
 Keywords: Foliar spraying with ZnSO4; Grapes enrichment with Zn; Nutrient’s interactions; Sugars and fatty acids profiles; Vitis vinifera L. variety Syrah; Winemaking.

The effect of commercial β-glucosidase and its immobilization on quality of red wine production

To find a feasible strategy for commercial β-glucosidase to enhance red wine quality, immobilized enzyme technology were considered. The preferred epoxy resin using the covalent binding method was ES-103B, and its optimal immobilization conditions were pH 4.9, 25 h immobilization time and 3 mg/g enzyme concentration. Meanwhile, this immobilized β-glucosidase still contained weak esterase activity just like commercial β-glucosidase. Free commercial β-glucosidase had different effects on the fermentation of 11 clones from three grape varieties (Syrah, Merlot and Cabernet Sauvignon) to produce wine. For example, the free enzyme increased the concentrations of β-damascenone, 1-hexanol, phenylethyl alcohol, and three ethyl esters (ethyl hexanoate, ethyl octanoate and ethyl decanoate) in a Cabernet Sauvignon (CS169) wine, but total anthocyanins level was reduced, affecting the color depth (a* value). Notably, during the alcoholic fermentation stage of CS169, immobilized β-glucosidase did not significantly decrease polyphenolic levels and color depth (a* value). What's more, using immobilized β-glucosidase under this treatment condition resulted in the highest levels of higher alcohols, ethyl esters, and acetate. Moreover, β-damascenone content increased by more than 25.35%, as well as phenethyl alcohol and benzaldehyde of benzenic compounds significantly increased by over 72.23% and two-fold, respectively. As a result, CS169 wine possessed more complex aroma and highest sensory score. Therefore, enologists can use immobilized β-glucosidase to develop various strategies that enhance wine quality and produce different wine styles.

Enhance Wine Production Potential by Using Fresh and Dried Red Grape and Blueberry Mixtures with Different Yeast Strains for Fermentation.

Red grapes and blueberries are known for their high content of bioactive compounds and antioxidant properties. In Mediterranean winemaking, traditional sun-drying can be replaced by controlled-airflow-chamber-drying, which provides better quality, higher phenolic content, and increased antioxidants. This study aimed to increase the sugar content and phenolic compounds of the must by drying the fruits to fifty per cent of their original moisture content. Two musts were prepared: the first one was prepared by combining fresh red grapes and dried blueberries (M1), while the other was created using dried red grapes and fresh blueberries (M2), followed by fermentation at 25 °C with M05 Mead and X5 yeast strains. The M2 must showed the highest levels of phenolic compounds, red color (A520), total anthocyanins, and antioxidant activity. During fermentation, the anthocyanin content increased mainly in the dried blueberry macerates, where it increased between 4- to 5.5-fold. More bioactive compounds were extracted from the wines produced using yeast inoculation despite the shorter maceration times. A sensory analysis demonstrated consumers' acceptance of the wines in terms of color, flavor, and aroma. In conclusion, the use of red grapes in the production of blueberry red wine proved to be effective, providing higher sugar and must yields, while the dried fruits improved the fermentable sugar content obtaining wines with an alcoholic content between 10 and 11% (v/v). The higher levels of bioactive compounds increased the antioxidant capacity of the resulting red fruit wines.

Applying Different Vinification Techniques in Teran Red Wine Production: Impact on Bioactive Compounds and Sensory Attributes.

Six different vinification treatments, including a control treatment (7-day standard maceration) (K7), were performed to study the effects of non-standard techniques on bioactive compounds and sensory attributes of Teran red wine. Pre-fermentative mash cooling (8 °C; 48 h) and heating (50 °C; 48 h) followed by prolonged post-fermentative maceration of 13 days (C15;H15) or 28 days (C30;H30) were applied. In another treatment, after cooling, saignée was performed followed by 13-day prolonged maceration (CS15). Wine phenols and vitamins were analyzed by HPLC-DAD-FLD, minerals by ICP-OES, and sensory analysis was performed using the QDA and 100-point O.I.V./U.I.O.E. methods. Obtained results showed total phenolic concentration was the highest in the H30 treatment. The concentration of anthocyanins, flavan-3-ols and phenolic acids was significantly higher in wines of all vinification techniques compared to the control. Stilbene content was highly affected by pre-fermentative heating. Treatments CS15, H15, C30 and H30 resulted in the highest scores by both the QDA and 100-point sensory methods. The obtained results suggest that advanced non-standard vinification techniques have a significant impact on Teran wine by enhancing its composition of bioactive compounds and improving its sensory profile, which gives it an additional market value. Furthermore, a comprehensive comparison of such techniques applied simultaneously in one study is of substantial importance for additional research in wine production.

Effects of Leaf Removal on Free and Glycoconjugate Aromas of Skins and Pulps of Two Italian Red Grapevine Varieties.

Leaf removal is a cultural practice mainly aimed at improving cluster zone microclimates and impacting primary and secondary metabolites, such as volatiles. This research aimed to assess the impact of defoliation on free and glycosylated aromas of a neutral ('Nebbiolo') and a semi-aromatic ('Aleatico') red variety. Defoliation was performed at fruit set (BBCH 71) and, for 'Nebbiolo', also at berries touch (BBCH 81) phenological stages. Skins and pulps were separately analyzed by Solid Phase Extraction-Gas Chromatography/Mass Spectrometry. Results showed that the response to defoliation was variety-dependent. For 'Nebbiolo', especially when performed at the berries' touch stage, defoliation had a significant effect on the accumulation of free volatiles and glycosidic precursors. Differently, free and bound 'Aleatico' volatiles were less impacted by defoliation. Interestingly, in both grapevine varieties, defoliation significantly enhanced the accumulation of aroma precursors in grapes' skins, which is of particular relevance for red wine production and their aging potential. Moreover, results could be helpful for the management of grape quality, as defoliation is currently considered as a strategy to address climate change issues.

Alzheimer's Disease Genetic Influences Impact the Associations between Diet and Resting-State Functional Connectivity: A Study from the UK Biobank.

Red wine and dairy products have been staples in human diets for a long period. However, the impact of red wine and dairy intake on brain network activity remains ambiguous and requires further investigation. This study investigated the associations between dairy and red wine consumption and seven neural networks' connectivity with functional magnetic resonance imaging (fMRI) data from a sub-cohort of the UK Biobank database. Linear mixed models were employed to regress dairy and red wine consumption against the intrinsic functional connectivity for each neural network. Interactions with Alzheimer's disease (AD) risk factors, including apolipoprotein E4 (APOE4) genotype, TOMM40 genotype, and family history of AD, were also assessed. More red wine consumption was associated with enhanced connectivity in the central executive function network and posterior default mode network. Greater milk intake was correlated with more left executive function network connectivity, while higher cheese consumption was linked to reduced posterior default mode network connectivity. For participants without a family history of Alzheimer's disease (AD), increased red wine consumption was positively correlated with enhanced left executive function network connectivity. In contrast, participants with a family history of AD displayed diminished network connectivity in relation to their red wine consumption. The association between cheese consumption and neural network connectivity was influenced by APOE4 status, TOMM40 status, and family history, exhibiting contrasting patterns across different subgroups. The findings of this study indicate that family history modifies the relationship between red wine consumption and network strength. The interaction effects between cheese intake and network connectivity may vary depending on the presence of different genetic factors.