Cell cultures of Plumbago rosea L. (synonymous P. indica) were treated with the elicitors prepared from the fungi ( Aspergillus niger and Rhizopus oryzae), bacteria ( Bacillus subtilis and Pseudomonas aeruginosa), yeast extract and chitosan to induce and enhance the synthesis of plumbagin. Elicitation of plumbagin production in chitosan treated cells was 6.71-fold higher compared to control cells. The treatment of cells with A. niger, R. oryzae and yeast elicitors resulted in two- to three-fold more plumbagin over control cells. Bacterial elicitors did not show much (<two-fold) influence on plumbagin accumulation. Chitosan at 150 mg/l dose level enhanced permeability of plumbagin from the cell to the exterior and also elicited plumbagin synthesis significantly.