Mushroom Production Research Articles

Performance of oyster mushroom (Pleurotus ostreatus) on paddy straw, water hyacinth and their combinations

This study aimed to investigate the performance of oyster mushrooms on different substrate combinations, including rice straw alone, rice straw and water hyacinth (1:1), rice straw and water hyacinth (1:2), and rice straw and water hyacinth (2:1), in Pokhara, Kaski from April to June in 2022. A Completely Randomized Design with four replications was used to analyze the growth and yield parameters of the four different substrate combinations. The data were analyzed using R-Studio. Highly significant results (P > 0.001) were found in all growth and yield parameters. The best response in terms of days to colonization, pinhead formation, and total crop duration was observed with rice straw alone (11.5, 13.25, and 54.00 days, respectively). The stipe length, pileus diameter, and fruiting body per bunch had their highest values (3.44 cm, 5.21 cm, and 34.50 cm, respectively) with rice straw alone, followed by rice straw and water hyacinth in a 2:1 ratio (3.05 cm, 4.63 cm, and 34.25 cm, respectively). The maximum total fresh yield of mushrooms (1.53 kg with 153.16% BE) was observed for rice straw alone, followed by rice straw and water hyacinth in a 2:1 ratio (1.17 kg with 116.5% BE), while the poorest yield (0.76 kg with 76.38% BE) was observed in the case of rice straw and water hyacinth in a 1:2 ratio. The highest profitability was observed for mushroom production using rice straw alone with a B:C ratio of 1.24, followed by using rice straw and water hyacinth in a 2:1 ratio (1.03), while the lowest B:C ratio was observed for rice straw and water hyacinth in a 1:2 ratio (0.74). Overall, the best treatment was found to be rice straw alone, followed by rice straw and water hyacinth in a 2:1 ratio. These results suggest that using rice straw alone or in combination with water hyacinth in a 2:1 ratio can be an effective strategy for oyster mushroom cultivation.

Utilization of Lignocellulosic Substrates on Oyster Mushroom (Pleurotus citrinopileatus) Cultivation

Background: Mushroom cultivation increased throughout the world with the utilization of crop residues. Oyster mushroom (Pleurotus spp.) is suitable for most of the area and less expensive for cultivation with the availability of paddy straw. Increasing demand of paddy straw compels to search alternate source of substrate for mushroom production. Based on this information efforts were taken to compare many locally available leaf substrates for Pleurotus citrinopileatus production. Methods: Leaf substrates viz., teak, bamboo, maize, banana, coconut, mango, sugarcane bagasse and grass (Dactyloctenium aegyptium) were tested for the cultivation of P. citrinopileatus. Grain substrates viz., paddy, cumbu, bengal gram, green gram, sorghum, black gram, ragi and the above leaf substrates were studied for their effect on radial mycelial growth and dry weight of P. citrinopileatus. Result: All the leaf substrates were on par with each other for radial mycelial growth. Highest mycelial dry weight of 724.67 mg was recorded in paddy straw. Among the different leaf substrates used for mushroom bed preparation, banana leaf substrates showed highest yield of 115.00 g than paddy straw. Weight of mushroom spent bed was recorded to observe the utilization of substrates in which bamboo was decomposed at the maximum. Addition of grain substrates enhanced the growth of mycelium.

Increasing the Capacity of Mushroom Production and Their Processing in the “Argodadi Pinilih” Disabled Group

The disabled group "Argodadi Pinilih" has 40 active members and already has a complete board. The Covid 19 pandemic caused some members to lose their jobs. This situation has encouraged members of the disabled group to take up other businesses, including oyster mushroom cultivation. The oyster mushroom business is carried out in a mushroom house which is placed inside the house or yard and the maintenance activities are carried out by the members of the disabled group themselves. The results of oyster mushrooms are all sold to traders around the house because the production of oyster mushrooms is still small. The problems faced by partners are (a) the production capacity of oyster mushrooms is still low, (b). limited skills in processing food from mushrooms, (c). marketing of oyster mushrooms and their processing is still limited. The solutions and output targets to be achieved are (a) increasing the production capacity of oyster mushrooms (b) increasing skills in the manufacture of processed food made from oyster mushrooms, and (c) expanding the marketing of oyster mushrooms. The activities carried out were (a) counseling and technical training on oyster mushroom cultivation as well as providing 2,200 baglogs, (b) training on processing food made from oyster mushrooms, namely in the form of crispy mushrooms and mushroom satay, and providing oil slicer (spinner), and (c) counseling on strategies online marketing and promotion. The results of the dedication showed that there was an increase in the production of oyster mushrooms produced, group members also became skilled in processing oyster mushrooms into crispy mushrooms and mushroom satay. The mushroom market share was getting wider, for starting to promote mushrooms through WhatsApp online media. Group members' knowledge of mushroom cultivation and marketing strategies increased, as evidenced by the pre-test results of 78.46 and 88.46 post-test with an increase of 12.75%.

Substrate Optimization for Shiitake (Lentinula edodes (Berk.) Pegler) Mushroom Production in Ethiopia.

Edible mushrooms are seen as a way of increasing dietary diversity and achieving food security in Ethiopia. The aim of this study was to develop substrates using locally available agro-industrial by-products and animal manures to enhance the production of Shiitake (Lentinula edodes) mushrooms in Ethiopia. The hypothesis was L. edodes mushroom production on seven different substrates: 100% sugarcane bagasse (S1), 80% sugarcane bagasse, 20% cow dung (S2), horse manure (S3), chicken manure (S4), cottonseed hulls (S5), sugarcane filter cake (S6), and sugarcane trash (S7). Mushroom yield and biological efficiency were significantly affected by substrate type (p < 0.05). A significantly higher yield (434.33 g/500 g of substrate) and biological efficiency (86.83%) were obtained using substrate S4 while lower yield (120.33 g/500 g) and biological efficiency (24.33%) were obtained using substrate S7 than when using other substrates. The largest first flush of mushrooms was obtained on S4, and five flushes were produced on this substrate. S4 also had the highest biological efficiency, the highest nitrogen content, and the lowest C:N. Chicken manure is rich in nitrogen, magnesium, calcium, and potassium, which are crucial for Shiitake mushroom growth. Thus, substrate S4 would be a viable option for cultivating Shiitake mushrooms, particularly in regions where chicken manure is readily available. Substrate S2 also provided high yields and rapid fructification and would be a suitable alternative for Shiitake mushroom cultivation.

Effect of UV‐B irradiation on bioconversion of ergosterol to vitamin D2 and its impact on nutritional properties of oyster mushroom (Pleurotus florida)

SummaryVitamin D deficiency is a global concern for vegetarians as most of the vitamin D‐enriched foods are of animal origin. Pleurotus florida is an excellent source of ergosterol, which gets converted into vitamin D2 under UV exposure. The objective of this study was to optimise UV‐B intensity (1.00–2.50 W/cm2) and exposure time (20–120 min) for maximum ergosterol conversion to vitamin D2. The optimisation studies indicated that exposure with intensity (2.00 W/cm2) for 60 min showed maximum vitamin D formation. The analysis of different nutritional parameters revealed no significant impact on total soluble proteins while a significant increase in total phenolic and total flavonoid contents was observed. On the contrary, a significant reduction was observed in riboflavin content of P. florida. Overall, P. florida exposure to UV‐B is a sustainable approach to enhance the vitamin D content for the development of fortified mushroom products or dietary supplements.

Microbiological quality and safety of fresh mushroom products at retail level in Korea.

Several investigations and recalls have demonstrated that Listeria monocytogenes can occur on mushrooms. This study aimed to assess the microbiological quality and safety of four types of edible mushrooms (Flammulina velutipes, Pleurotus ostreatus, Pleurotus eryngii, and Agaricus bisporus) available in the Korean market, and to evaluate the prevalence of Listeria spp., including L. monocytogenes. Results revealed that out of 100 samples tested, 16% (32/200) were positive for Listeria spp. Of the Listeria-positive samples, five strains of Listeria innocua were detected. The total microbial counts ranged from 0.79 to 5.84 log CFU/g, with F. velutipes exhibiting the highest microbial load (mean 5.03 log CFU/g). These findings provide significant data for risk assessment and emphasize the need for continued monitoring of the microbiological safety of edible mushrooms.

- Increasing the Independence of Oyster Mushroom (Pleurotus ostreatus) Entrepreneurs in Seedlings Preparation

Sadam Mushroom House is one of the mushroom producers in Medan Marelan with oyster mushroom production of 7-12 kg/day and can accommodate 7,000 baglogs. The problem of the oyster mushroom business is still very dependent on mushroom nurseries in an effort to provide F2 seedlings because there are still very limited facilities, infrastructure, understanding and skills in providing F0 - F2 seeds. Another problem is that the baglogs waste produced (2,000-3,000 baglogs every 3 months) has not been used properly so that it has the potential to pollute the environment. The purpose of this community service is to increase the independence of partners in providing oyster mushroom seeds, making compost from baglog waste and its use in plant cultivation. The solutions offered in solving these problems are the introduction of appropriate technology in the supply of F0-F2 mushroom seeds, the introduction of technology transfer goods, the management of baglog waste into compost and the use of compost from baglog waste as organic fertilizer for plant cultivation. The results of the community service that have been achieved are the introduction of technology transfer goods in the form of an autoclave, simple laminar air flow and tools for providing F0-F2 seedlings, training and assistance in providing F0-F2 mushroom seedling, training and assistance in making compost from baglog mushroom waste, direct practice of using mushroom compost for cultivating mung bean crop.

Keragaan Agribisnis Usaha Jamur Tiram Putih di Kelurahan Anduonohu Kecamatan Poasia Kota Kendari (Studi Kasus di Usaha Tiga Putra Farm)

Oyster mushroom is a type of wood mushroom that Indonesia commonly consumes. It has a fruiting body that grows in bloom to form a shallow funnel shell (oyster). This study aimed to determine the agribusiness performance of the White Oyster Mushroom (Pleurotus ostreatus) in Anduonohu ​​Village, Poasia District, Kendari City. Case study on Tiga Putra Farm Business. This research was conducted in September 2021. The object of this research was the Tiga Putra Fram Timber Mushroom business. The method used in this study is Qualitative Descriptive Analysis. The type of data in this study used primary data, namely direct interviews, and secondary data, namely library literature. The variables used in this study are 1. The informant's identity includes age, education level, business experience, and some family dependents, 2. Agribusiness performance from this study is in the form of support in the agribusiness subsystem, which includes production facilities (seeds, fertilizers, tools /machinery, and mushroom house), cultivation and farming subsystem (planting, maintenance, processing), and marketing subsystem (distribution). The results of this study are the agribusiness performance of the white oyster mushroom (Pleurotus ostreatus), namely the procurement and provision of production facilities, including the procurement of mushroom houses, seeds, tools, and machines. Cultivation and farming include oyster mushroom production, maintenance, and harvest. Oyster mushroom product marketing is done online and offline by online method, namely posting oyster mushroom products on several social media platforms such as Facebook, WhatsApp, and Instagram. Thus the range of product marketing can be more comprehensive. Offline marketing to resellers of oyster mushrooms that will be resold, such as the Ummushabri Kendari and Hypermart.

Examining the Impact of Substrate Composition on the Biochemical Properties and Antioxidant Activity of Pleurotus and Agaricus Mushrooms

The composition of the substrate is one of the most critical factors influencing the quality as well as the nutritional value and bioactive content of mushrooms. Therefore, the effects of various substrates, such as barley and oat straw (BOS), beech wood shavings (BWS), coffee residue (CR), rice bark (RB) and wheat straw (WS, control substrate), on the biochemical properties (lipid, protein, polysaccharide, glucan, ash, and mineral content, fatty acids and tocopherols composition), total phenolic compounds and antioxidant activity of Pleurotus mushrooms, P. ostreatus (strains AMRL 144, 150) and P. eryngii (strains AMRL 166, 173-6), cultivated in ‘bag-logs’, was examined. Proximate analysis of A. bisporus and A. subrufescens grown on two different composts (C/N ratios of 10 and 13) was conducted, too. The whole carposomes, pilei and stipes were analyzed. Results showed that BOS, RB, BWS and CR improved the antioxidant activity of Pleurotus species and their nutritional characteristics. Both pilei and stipes were rich in polysaccharides (27.51–67.37 and 22.46–39.08%, w/w, for Pleurotus and Agaricus spp., respectively), lipids (0.74–8.70 and 5.80–9.92%, w/w), proteins (6.52–37.04 and 25.40–44.26, w/w, for Pleurotus and Agaricus spp., respectively) and total phenolic compounds (10.41–70.67 and 7.85–16.89 mg gallic acid equivalent/g for Pleurotus and Agaricus spp., respectively), while they contained important quantities of unsaturated FAs of nutritional and medicinal importance. Pilei were richer in proteins, total phenolic compounds and enhanced antioxidant activity and reducing power than stipes, whereas stipes were richer in IPSs and glucans compared to the corresponding pilei. Thus, mushroom cultivation could upgrade rejected agro-industrial residues and wastes to new uses as substrates for the production of mushrooms with specific nutritional and medicinal attributes.

Pemberdayaan Kelompok Difabel “Argodadi Pinilih” Berbasis Usaha Tani Jamur

Disabled group "Argodadi Pinilih" has 40 active members. The COVID-19 pandemic has caused some members to lose their jobs. Members of the disabled group began to try to cultivate oyster mushrooms in their homes and they also carried out their own maintenance. Some “baglog” cannot grow mushrooms because the environment where the mushrooms grow does not meet the requirements. The problems faced by the disabled group "Argodadi Pinilih" are low quantity and quality of oyster mushrooms, limited skills in making processed food from mushrooms and mushroom marketing is still limited. The objectives of this community empowerment activity are (a) increasing the quantity and quality of mushroom production (b) improving skills in the manufacture of processed food made from mushrooms, (c) expanding the mushroom market share. The methods used in the implementation of empowerment activities are (a) counseling on oyster mushroom cultivation and marketing strategies (b) training on processing food from oyster mushrooms, (c) facilitation house of mushroom and baglog. The results of the empowerment show that the training, mentoring and facilitation of mushroom house and “baglog” can increase the quantity and quality of oyster mushroom production, improve the skills in making processed food from mushrooms and expand the market share of oyster mushrooms. The knowledge and skills of the disabled group members about mushroom farming and processing increased as evidenced by the pre-test score of 79.23 and post-test of 86.00 with an increase 8.54%.

The Effect of Population Level and Technology toward the Oyster Mushroom Production

The study was conducted during the year 2018-19 in Raipur and Dhamtari district of the Chhattisgarh state. The reason for selecting the districts because Front Line Demonstration of particular oyster mushroom production was conducted in these districts. The stakeholders like researchers, progressive growers, business personnel and self-help group of oyster mushroom production were selected, in this way the total 60 stakeholders were selected for the study. The data were collected by personally interview with the help of pre-tested interview schedule. A list of variables to be dealt with was prepared based on Adoption Diffusion Outcome Prediction Tool which having four quadrants that were considered for analysis are as follows: (1) Relative advantage for the farming population, (2) Learnability characteristics of the innovation, (3) Population-specific influences on the ability to learn about the practice, and (4) Relative advantage of the technology [1] and collected data were classified, tabulated and analysed using descriptive statistics like mean, frequency, and percentage. Majority of respondents were oriented towards income increment and profit maximization. oyster mushroom production enterprise is coming under the 'easily trialable' category (60.00%) followed by 'moderately trialable’ category (25.00%). With regarding to ease and convenience, the results from highlights that oyster mushroom production considered under ‘small increase in ease and convenience’ category (38.33%) followed by ‘large increase in ease and convenience’ category (30.00%).

Sebagai Upaya Meningkatkan Pendapatan Masyarakat Desa Wonomerto Melakukan Budidaya Jamur Tiram Putih

Indonesia, with a hot climate and high humidity, is an ideal place for the growth of various types of mushrooms. And also, cultivating oyster mushrooms is quite easy when compared to others. Apart from that, the main advantage of oyster mushroom cultivation is that it does not require extensive permits, has a long relatively short harvest of about one to three months, and oyster mushroom seeds are easy to obtain at an affordable price. In this research, the Participatory Action Research (PAR) method is used, which is a research approach that involves the active participation of people involved in the context being studied. It is hoped that with the cultivation of oyster mushrooms in Wonotirto Hamlet which continues to grow, the situation can change so that it can increase the income of local residents. With the development of oyster mushroom cultivation in Indonesia, especially Wonomerto Village, it is hoped that the number of oyster mushroom farmers or entrepreneurs can continue to increase so that oyster mushroom production can also increase.&#x0D;

Biochar immobilized plant growth-promoting rhizobacteria enhanced the physicochemical properties, agronomic characters and microbial communities during lettuce seedling.

Spent mushroom substrate (SMS) is the by-products of mushroom production, which is mainly composed of disintegrated lignocellulosic biomass, mushroom mycelia and some minerals. The huge output and the lack of effective utilization methods make SMS becoming a serious environmental problem. In order to improve the application of SMS and SMS derived biochar (SBC), composted SMS (CSMS), SBC, combined plant growth-promoting rhizobacteria (PGPR, Bacillus subtilis BUABN-01 and Arthrobacter pascens BUAYN-122) and SBC immobilized PGPR (BCP) were applied in the lettuce seedling. Seven substrate treatments were used, including (1) CK, commercial control; (2) T1, CSMS based blank control; (3) T2, T1 with combined PGPR (9:1, v/v); (4) T3, T1 with SBC (19:1, v/v); (5) T4, T1 with SBC (9:1, v/v); (6) T5, T1 with BCP (19:1, v/v); (7) T6, T1 with BCP (9:1, v/v). The physicochemical properties of substrate, agronomic and physicochemical properties of lettuce and rhizospheric bacterial and fungal communities were investigated. The addition of SBC and BCP significantly (p < 0.05) improved the total nitrogen and available potassium content. The 5% (v/v) BCP addiction treatment (T5) represented the highest fresh weight of aboveground and underground, leave number, chlorophyll content and leaf anthocyanin content, and the lowest root malondialdehyde content. Moreover, high throughput sequencing revealed that the biochar immobilization enhanced the adaptability of PGPR. The addition of PGPR, SBC and BCP significantly enriched the unique bacterial biomarkers. The co-occurrence network analysis revealed that 5% BCP greatly increased the network complexity of rhizospheric microorganisms and improved the correlations of the two PGPR with other microorganisms. Furthermore, microbial functional prediction indicated that BCP enhanced the nutrient transport of rhizospheric microorganisms. This study showed the BCP can increase the agronomic properties of lettuce and improve the rhizospheric microbial community.

Agro Composite Waste: A Novel and Economical Substrate for the Production of Edible Mushroom

Agro Composite Waste: A Novel and Economical Substrate for the Production of Edible Mushroom

Improving the efficiency of management decision-making: developing a simulation model of a cultivated mushroom farm

Industrial production of edible mushrooms is now regarded as an independent agricultural industry not dependent on the seasonality of work inherent in most agricultural operations. Since agricultural production is characterized by high consumption of resources, cultivation and harvesting of mushroom produce are carried out by means of specialized technical and technological equipment, allowing one to provide the necessary microclimate conditions. These issues can be solved by using intensive technological resource-saving processes, which provide a sufficient level of production profitability. In this regard, there is a need to develop an economic-mathematical model able to simulate the process of the enterprise as an example. The study aims to develop a simulation model of a mushroom farm to improve the effectiveness of management decisions. The study employs the AnyLogic simulation instrument to create a model and test its accuracy with the cultivation of Pleurotus ostreatus oyster mushrooms. The authors develop a model of a farm for growing cultivated mushrooms and describe the created model, the algorithm of its operation, and the created interface. This model gives an idea about the optimal conditions for the cultivation of mushrooms and can be used for decision-making in techno-management complexes.

Recycling of spent Oyster mushroom (Pleurotus ostreatus) sawdust waste for the production of Straw mushroom (Volvariella volvacea)

The cultivation of straw mushroom Volvariella volvacea has a long history and has undergone continuous development. Nevertheless, ongoing research aims to utilize various agricultural wastes to improve biological efficiency. In this study, we investigate a sustainable strategy that promotes the conversion to a circular economy by recycling spent mushroom substrate in new mushroom crops. Our research provides insight into reducing spent mushroom sawdust waste (SMSW) of Pleurotus ostreatus for application in Volvariella volvacea cultivation. We found that the optimal conditions for cultivating V. volvacea were at a temperature of 35.0℃ a humidity of 80-85%, resulting in an average growth rate of 10.60 cm and a total weight of fruit bodies of 0.77 kg, with a biological efficiency of approximately 15.47% within 15 days of cultivation. We discovered that the SMSW could be an alternative substrate for V. volvacea cultivation. Our findings can be applied to improve V. volvacea cultivation and practical applications in agriculture. By involving innovative waste management practices, this article can foster economic growth while preserving the environment for future generations. Therefore, this study contributes to significant socio-economic development and supports green economic policies by discussing the improvements from using SMSW.

Mushroom based Cosmeceuticals: An Upcoming Biotechnology Sector

ABSTRACT: Mushrooms have been an established source of nutrition for hundreds of years. They are recently being exploited for their potential applications in other sectors as well. One of their emerging applications has been in the cosmetic industry where their bioactive properties are being used either as cosmeceutical for topical administration or as nutricosmetics for oral administration. Certain genera of mushrooms which are being used in cosmetic industries are Lentinula, Ganoderma, Pleurotus, Cordyceps, Inonotus, Tremella, Grifola, Schizophyllum, Pleurotus, Coriolus, Ganoderma, Euphorbia, Pistacia, Trametes, Agaricus, Phellinus, Antrodia. The mushroom extracts and their bioactive metabolites show excellent anti-oxidant, anti-wrinkle, anti-aging, moisturizing and skin whitening effects making them ideal and multi-functional cosmetic components. The mounting benefits of various mushroom extracts in skin and hair care products have also fast-tracked the commercial production of mushrooms which is worth mass attention. The present review discusses about the role, diversity, properties and commercial status of mushrooms that are being exploited by the cosmetic industry.

Influence of the chemical content of sawdust on the levels of important macronutrients and ash composition in Pearl oyster mushroom (Pleurotus ostreatus).

Influence of chemical composition of sawdust on the nutritional profile of oyster mushrooms (Pleurotus ostreatus) has yet to receive significant research attention. This information will help mushroom growers to select specific sawdust for the production of mushroom with desired dietary preferences. This study assessed the influence of the chemical composition of sawdust on the macronutrients and ash content of the pearl oyster mushrooms. The American Standard for Testing Materials and other widely accepted protocols were used to determine the C-N ratio, pH, lignin, hemicellulose and cellulose contents of mixed sawdust from tropical wood species. The study evaluated the fat, crude fibre, crude protein, carbohydrate, and ash content of the oyster mushroom cultivated on the sawdust. Cellulose constituted the largest component of the sawdust (47.82%), followed by lignin (33.29%). The yield of the mushroom (on 0.05 kg of sawdust) ranged from 490.1 to 540.9 g (biological efficiency: 44-50%); the average carbohydrates constituent in the mushroom was 56.28%. pH of the sawdust influenced the crude protein, carbohydrate, fat and ash content of oyster mushrooms (p<0.05) most significantly. The hemicelluloses also had a significant effect (p<0.05) on the mushroom's minerals, fat and crude fiber content. The study revealed that the mushroom producers would likely obtain high protein content using sawdust with low pH (slightly acidic to slightly basic) in the oyster mushroom. Mushrooms grown on substrates, rich in hemicelluloses, had low fat and high crude fiber content.

Economic Analysis of Shiitake Mushroom Cultivation as an Agribusiness Enterprise in India

"With a great deal of constraints such as heavy dependence on weather, small and fragmented land holdings, depleting resource, demand shift etc. faced by traditional Indian agriculture, farmers are shifting towards allied enterprises. Mushroom is one such enterprise which provides remunerative and nutritive produce with less-land and investment requirements. Hence, the present study assessed the economic performance of the Shiitake mushroom production in terms of cost &amp; return analysis, viability analysis and BEP assessment using primary data. The overall net returns were ` 235/ bag/ cycle of Shiitake with rate of return on capital of 1.93 showing the economic viability of the enterprise. It was also found that larger units of Shiitake mushroom were more viable and economically sound as compared to smaller and medium units. Since lesser land and other resources are required for Shiitake mushroom with higher returns and good nutritive value, such mushrooms ought to be promoted for adoption at large scale to alleviate poverty and nutritional insecurity of vulnerable Indian population."

Pilot Study of Diet Supplemented with Sold-Out Substrate of Pleurotus ostreatus in the Feeding of Backyard Broilers

There are several by-products obtained in agricultural systems that can contribute to small-scale production systems within backyards, in this sense, the production of oyster mushrooms (Pleurotus ostreatus) has been integrated into the rural family economy in Mexico. After harvesting the fruiting bodies, the remainder is known as sold-out substrate, this by-product has been destined for other agricultural uses such as: medium for seedlings, vermiculture or fertilizer; however, there are studies where it has been used in the diet of bovine cattle. In this study, the effects of sold-out substrate (corn stubble) of P. ostreatus in the diet of broilers with different percentages of protein, on feed conversion ratio, carcass yield, and broiler meat quality were evaluated. A total of 120 broilers Ross 308 were randomly assigned in 12 pens with concrete floor and curly shaving with an area of 3 m2. During the initial period (1 to 28 days), all broilers were fed commercial feed® mixture. On day 29, the broilers were weighed and divided into four treatment groups and fed diets with different percentages of protein until the end of the experiment. Feed conversion ratio was significantly higher in treatment control (TC) compared to the other treatments; however, the performance parameters were not affected by the diet supplemented with sold-out substrate, likewise, the color and quality of the meat were in a normal range (48 &lt; L* &lt; 51) and with a good amount of crude protein. Sold-out substrate of P. ostreatus from corn stubble presented a high mineral content of Mg, Na, K, Fe, Cu, and Mn suitable to be considered in diets for feeding backyard broilers.