The substrate used in the production of seedlings has a key role in early development of the plant. The objective of this study was to assess the potential of vermicompost as substrates for the production and development of seedlings of lettuce (L. sativa L.). The test was conducted in the greenhouse of the Department of Soils, Universidade Federal de Santa Maria, divided into 10 treatments and seven of ten repetitions. The treatments: T1-Red Residue (RVM), T2-Green Residue (RVD), T3-Residue Red: rice Husk “in nature” – 1: 1 (RVM: CAN 1: 1), T4-Residue Green: Rice husk “in nature” – 1: 1 (RVD: CAN 1: 1), T5-Residue Red: rice Husk Carbonized – 1: 1 (RVM: CAC 1: 1), T6 Residue Green: Rice husk Carbonized – 1: 1 (RVD: CAC 1: 1), T7-Residue Red: Residue green line – 1: 1 (RVM: RVD 1: 1), T8-Residue Green: Rice husk “in nature” – 3: 1 (RVD: CAN 3: 1), T9-Residue Red: rice Husk Carbonized – 3: 1 (RVM: CAC 3: 1) and T10 Fertile Turf (EB). Treatments that had better conditions on the number of leaves, height, fresh and dry biomass production of seedlings was treated with 50% residual red: 50% green waste - RVM: RVD. For the production of lettuce showed that the treatment is effective for plant height was RVM: RVD. For the other treatment variables RVM: CAC had better conditions when we compared the treatment that the CAC was in smaller proportions. It can be inferred that the RVM RVD and mixed with rice hulls and rice husk “fresh” can be used for the production of lettuce seedlings.