The study aimed to determine the effect of leaf maturity on nutritional composition, bioactive compounds, minerals and antioxidant activity in fourteen genotypes of Moringa oleifera grown in the subtropical climate of north-western India. Significantly (p≤0.01) high protein, fibre, carotenoids, antioxidant activity, tannins, saponins and almost all essential elements except Copper and Zinc were observed in the mature leaf stage. However, tender leaves were found the best for carbohydrates, sugars, phenols and flavonoids. The elevated antioxidant capacity shown by the Ferric-reducing antioxidant power and 2,2-diphenyl-1-picryl hydrazyl free radical scavenging techniques was directly correlated with the elevated phenolic compound concentration. Significantly higher protein and fibre were analyzed in S12; fats, carbohydrates and total energy in S14 genotype. Huge variations were observed for bioactive compounds and minerals. Among the genotypes assessed, S6 (tender leaves) and S2 (mature leaves) exhibited a more balanced nutritional profile. Thus, these findings confirm the relevance of nutritional quality of M. oleifera in assessing its suitability as a source of nutrients, antioxidants, bioactive ingredients and minerals, can provide a reference for growers to choose the best cultivar and judicious mixing of tender and mature leaves in certain proportions can produce raw materials for the production of value-added goods, and because of their possible immune-boosting effects, they may also contribute to giving health advantages and fortifying foods.