Fermented Milk Products Research Articles

Analysis of actual nutrition of schoolchildren at different age periods

Introduction. A healthy, balanced diet can provide the child with nutritional protection from adverse environmental factors and helps to prevent diseases. Lack of continuity in nutrition at school and at home creates a deficit in the diet supply of nutrients necessary for a growing body. The purpose of the investigation is to study the actual nutrition of a group of schoolchildren at different age periods (in the dynamics of 10 years). Materials and methods. Actual nutrition of a group of schoolchildren in the dynamics over 2009 and 2019. The sample consisted of one hundred fifty 7-years children and the same children aged 17 years (124 children). Actual nutrition was studied using menu-layouts (at school) and the method of 24-hour (daily) nutrition reproduction (at home). Results. With age, there is a more pronounced imbalance in the supply of basic nutrients in the diet, associated with excess consumption of fats (7–25%), lack of carbohydrates, deficiency of dietary fibers (15–20%), a number of minerals (calcium, magnesium, iron) and vitamins (C, B1, B2, A, PP). Average daily food rations are unbalanced. The structure of children’s food sets becomes more irrational with increasing age. Regardless of the period of the study, diets are deficient in the level of consumption of milk, fish, vegetables, and fruits, and excessive in the level of consumption of foods with high energy value. In dynamics, the percentage of children with the recommended level of consumption of poultry is decreasing by 4.3 times, fish – by 3.8 times, milk – by 3 times, fermented milk products – by 2.6 times, cottage cheese and sour cream – by 2 times, butter and vegetable – 15.4 and 6.8 times, respectively. Limitations. This study is limited to a sample of a group of schoolchildren studying from the 1st to the 11th grade (in the dynamics with a ten-year interval). Conclusion. Persistent long-term violations of the basics of a healthy balanced diet by children create a probable risk of developing diseases in children in the future caused by nutritional factors, and can also be predictors of the development of pathological conditions and diseases in adulthood.

Investigation on Fermented Milk Quality after the Addition of Flaxseed Mucilage and the Use of Lactobacillus delbrueckii subsp. bulgaricus and Lactiplantibacillus plantarum AG9

Background: Flaxseed mucilage (FSM) is one of the healthy components of flaxseed. FSM is an example of a material that can be used in the food, cosmetic, and pharmaceutical industries due to its rheological properties. FSM consists mainly of two polysaccharides, arabinoxylan, and rhamnogalacturonan I, and it also contains protein components and minerals. The prospect of using FSM in food is due to its gelling, water binding, emulsifying, and foaming properties. In addition, valuable natural sources of phenolic compounds such as lignans, phenolic acids, flavonoids, phenylpropanoids, and tannins are partially extracted from flaxseed in FSM. These antioxidant components have pharmacological properties, including anti-diabetic, anti-hypertensive, immunomodulatory, anti-inflammatory and neuroprotective properties. A combination of FSM and lactobacilli in dairy foods can improve their functional properties. This study aimed to develop dairy products by adding of FSM and using two lactic acid bacteria (LAB). FSM (0.2%) was used as an ingredient to improve both the texture and antioxidant properties of the product. Methods: Skim milk was fermented with 0.2% flaxseed mucilage using Lactobacillus delbrueckii subs. bulgaricus and the probiotic Lactiplantibacillus plantarum AG9. The finished fermented milk products were stored at 4 °C for 14 days. Quantitative chemical, textural, and antioxidant analyses were carried out. Results: Adding 0.2% FSM to the dairy product stimulated the synthesis of lactic acid. FSM increased the viscosity and water-holding capacity of L. bulgaricus or L. bulgaricus/L. plantarum AG9 fermented milk products. Combining these starter strains with FSM promoted the formation of a hard, elastic, resilient casein matrix in the product. When only L. plantarum AG9 was used for the fermentation, the dairy product had a high syneresis and a low viscosity and firmness; such a product is inferior in textural characteristics to the variant with commercial L. bulgaricus. The addition of FSM improved the textural properties of this variant. The use of L. plantarum AG9 and FSM makes it possible to obtain a fermented milk product with the highest content of polyphenolic compounds, which have the highest antioxidant properties and stimulate lipase and α-glucosidase inhibitor synthesis. Combining of L. bulgaricus and L. plantarum AG9 in the starter (20% of the total mass of the starter) and adding of 0.2% FSM is the optimal combination for obtaining a dairy product with high textural and antioxidant properties. Conclusions: The physicochemical properties (viscosity, syneresis, water holding capacity, texture) and antioxidant properties of fermented milk were improved. In the future, as part of the work to investigate the functional properties of dairy products with FSM, studies will be conducted using in in vivo models.

Lactococcus lactis D4 Has Potential Effect to Alleviate Inflammation and Reverse Dysbiosis in Colitis Rat Model

BACKGROUND: Inflammatory bowel disease (IBD) is a prevalent chronic inflammatory disorder of the gastrointestinal tract that may lead to colorectal cancer if untreated. Several medications for IBD have adverse side effects. Thus, there are high interest for searching alternative treatment to treat IBD. Lactococcus lactis D4 isolated from dadih, a traditional fermented buffalo milk product, was investigated for the effect on gut inflammation and microbial composition in the colitis rat model.METHODS: Eighteen male Sprague-Dawley rats were divided into three groups; control rats, colitis-induced rats, and colitis-induced rats treated with L. lactis D4 isolate. The control group received water, while the colitis-induced rats were given azoxymethane (AOM) and dextran sodium sulphate (DSS). Rats’ feces were collected for the analysis of gut microbiota diversity by next-generation sequencing and for the measurement of transforming growth factor (TGF)-β, nuclear factor kappa-B (NF-κB), interleukin (IL)-6, and tumor necrosis factor (TNF)-α colonic expression using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).RESULTS: L. lactis D4 administration was able to reduce inflammation in colitis by decreasing IL-6 expression (0.87 vs. 0.73), while expression of TGF-β, NF-κB, and TNF-α were increased compared to the model group, suggesting a complex immunomodulatory effect. Gut microbiota sequencing revealed a similarity between the control and treatment groups, suggesting L. lactis D4 has great potential to ameliorate gut microbiota dysbiosis in colitis rats.CONCLUSION: L. lactis D4 has a beneficial effect in decreasing pro-inflammatory cytokines and is able to reserve dysbiosis in colitis rat model. Thus, L. lactis D4 might be used as a therapeutic agent for IBD.KEYWORDS: colitis, gut microbiota, inflammation, inflammatory bowel disease, Lactococcus lactis D4, probiotic, proinflammatory cytokines

Metabolomic analysis reveals Ligilactobacillus salivarius CCFM 1266 fermentation improves dairy product quality

Previous studies have demonstrated that Ligilactobacillus salivarius CCFM 1266 exhibits anti-inflammatory properties and the capability to synthesize niacin. This study aimed to investigate the fermentative abilities of L. salivarius CCFM 1266 in fermented milk. Metabonomic analysis revealed that fermentation by L. salivarius CCFM 1266 altered volatile flavor compounds and metabolite profiles, including heptanal, nonanal, and increased niacin production. Genomic investigations confirmed that L. salivarius CCFM 1266 possess essential genes for the metabolism of fructose and mannose, affirming its proficiency in utilizing fructooligosaccharides and mannan oligosaccharides. The addition of fructooligosaccharides and mannan oligosaccharides during the fermentation process significantly facilitated the proliferation of L. salivarius CCFM 1266 in fermented milk, with growth exceeding 107 colony-forming units (CFU)/mL. This intervention not only augmented the microbial density but also modified the metabolite composition of fermented milk, resulting in an elevated presence of advantageous flavor compounds such as nonanal, 2,3-pentanedione, and 3-methyl-2-butanone. However, its influence on improving the texture of fermented milk was observed to be minimal. Co-fermentation of L. salivarius CCFM 1266 with commercial fermentation starters indicated that L. salivarius CCFM 1266 was compatible, similarly altering metabolite composition and increasing niacin content in fermented milk. In summary, the findings suggest that L. salivarius CCFM 1266 holds substantial promise as an adjunctive fermentation starter, capable of enhancing the nutritional diversity of fermented milk products.

Probiotic yoghurt-like fermented milk product enriched with Lactobacillus desidiosus and Lactobacillus fermentum: proximate composition, physicochemical, microbiological, and sensory evaluation during refrigerated storage

The combination of multiple strains and multiple species in lactic acid bacteria-based fermented milk offers the potential for nutritional and sensory attributes, making it an attractive option for developing high-quality and health-promoting probiotic yoghurt-like fermented milk products. In the present study, four combinations of yoghurt-like fermented milk samples: Sample 1 (Control), Sample 2 (Lactobacillus desidiosus), Sample 3 (Lactobacillus fermentum), and Sample 4 (Lactobacillus desidiosus and Lactobacillus fermentum) were developed. Proximate composition (moisture, ash, protein, fat, carbohydrate), physicochemical (pH, titratable acidity, syneresis, total soluble sloid), microbiological (Lactobacillus bacteria, yeast and mold, and coliform bacteria), and sensory properties (color, flavor, texture, taste, and overall acceptability) were assessed under refrigerator conditions (4 °C) from 0 to 28 days of storage period. Proximate composition differed significantly (p < 0.05) among samples for moisture (49.34–79.32%), ash (1.14–0.31%), protein (3.72–3.21%), fat (11.23–5.62%), and carbohydrate (33.11–12.98%). Yoghurt-like fermented milk containing Lactobacillus desidiosus as single or in combination with Lactobacillus fermentum resulted in reduced pH levels (6.02–3.49), total soluble solid (41.66–24.66%) and increased syneresis (30.04–65.52%), titratable acidity content (0.42–1.62%). Single or combination of Lactobacillus desidiosus and Lactobacillus fermentum significantly (p < 0.05) reduced Lactobacillus bacteria (8.17–6.01 Log CFU/g), yeast, and mold (1.45–1.19 Log CFU/g). Additionally, none of the samples of probiotic yoghurt-like fermented milk showed any detectable coliform count, indicating the possibility of prolonging the shelf life. The sample prepared by Lactobacillus desidiosus had the highest sensory ranking (8.10) at day 0 and during storage than other samples. In conclusion, the inclusion of Lactobacillus desidiosus and Lactobacillus fermentum strains in the composition of production starter cultures holds great promise. The utilization of these enriched starter cultures enables the production of healthy food products that maintain their quality throughout the entire storage period.

Exopolysaccharides isolated from fermented milk-associated lactic acid bacteria and applied to produce functional value-added probiotic yogurt

The current study aimed to isolate and characterize the lactic acid bacteria (LAB)-derived exopolysaccharide (EPS) and apply it to enhance yogurt's functional and technological quality. After screening and isolation of 52 different LAB strains from fermented milk products, Lactobacillus (L.) rhamnosus KF5, L. plantarum C182, Pediococcus (P.) acidilactici CCFM18, and Leuconostoc (Leu.) mesenteroides F27, with high EPS production yield, were nominated for further evaluation. EPS-derived strains were evaluated based on production yield, protein content, antioxidant activity, emulsification, and anti-inflammatory ability. After comparing isolated EPS samples, the Leu. mesenteroides F27 products were chosen in 3 concentrations (100, 250, and 500 mg EPS/L) to supplement the yogurt. EPS at all concentrations enhanced LAB growth, water-holding capacity, viscosity, and antioxidant activity. The electronic nose and tongue revealed that EPS did not affect the yogurt flavor profile. The confocal laser scanning microscopy showed a homogenously distributed protein and EPS network in the supplemented yogurt samples. However, in the rheology and consumer analysis, the 250 mg/L concentration showed better attributes, such as higher consistency, lower flow behavior, and a higher flavor score. Leu. mesenteroides F27-derived EPS could be used as a biological additive to enhance yogurt's technological and functional quality.

Functional Food Innovation Based on Fermented Milk Products with Fortification of Various Types of Tea: A Review

Functional Food Innovation Based on Fermented Milk Products with Fortification of Various Types of Tea: A Review

A REVIEW ON CASIEN FROM VARIOUS MILK SAMPLES ITS USES AND IMPORTENCE

The milk proteins are the oldest and most widely consumed food proteins. There is currently a large interest in these substances, both in nutritional field and in technological application. Casein proteins are a family of proteins involved in the production of cheese and fermented milk. They are very important nitrogen compounds not only for the dairy products production, but they are also additives in medicine, and they have a technical use in cosmetics, paints and adhesives. : Milk is an important part of human life and supposed to be a nutritious food which contain about 80% proteins. Milk proteins consist of 80% casein (soluble protein), 2-8% lactose (milk fat) and remaining is whey (byproduct of cheese and casein manufacture). Casein is more important and contain almost all essential amino acids. The purpose of the present work was to estimate the amount of casein in different milk samples including natural milk (cow, buffalo, sheep and goat). Keywords: Milk powder, casein, Amino Acids, paint, cheese

Development of technology and research of properties of fermented milk product with celery

Fermented foods and beverages have nutritional and medicinal properties. Usually, the expansion of the range of fermented sour milk products is carried out in two directions: the first is the use in technology of new active leavening microorganisms, resistant to the factors of the gastric environment with a corrective effect on the intestinal microbiota; the second is the introduction of additives mainly of fruit and vegetable origin into the composition of the ready-made fermented milk product. Celery is a vegetable that can also be used to enrich a fermented milk product with necessary nutrients. After all, celery extracts have many nutraceutical properties, namely antioxidant, hypolipidemic, hypoglycemic, and antiaggregation properties. The work aimed to substantiate the need to introduce puree of celery leaves, stalks, and roots into yogurt and to develop a fermented product. During the research, generally accepted organoleptic, physicochemical, and microbiological methods were used. The scientific expediency of enriching a fermented milk product – yogurt with celery puree – has been substantiated. The addition of more than 20 % celery puree has a positive effect on the phenomenon of syneresis of the finished product since the amount of whey separated during filtration was 3–4 times less than in control. According to organoleptic indicators, the best samples were yogurts with a celery content of 25 to 30 %; besides, yogurt with celery (30 %) had a significant reserve of microbiological stability during storage at temperatures of +3 –+5 °С. In particular, this fermented milk product can be stored for 14 days without deterioration of quality and safety indicators. Therefore, it is proposed that puree from celery stalks, leaves, and roots be added to the yogurt production technology in the amount of 25–30 %. This will enrich this fermented milk product with biologically valuable dietary fibers.

The role of dietary fiber in functional nutrition

The process of nutrition is a function of the relationship between a person and the environment. Food should contribute to the adaptation of the human body to unfavorable conditions and, in addition to its main function – meeting the physiological needs of the human body for nutrients and energy, also perform preventive and therapeutic tasks. Recently, the popularity of healthy food has increased. Experts predict that the market for functional products is growing every year. In this regard, in many countries, in-depth research is being carried out on the structure, composition, properties of dietary fiber, and the technology of their use as one of the components in the creation of composite food products. Dietary fiber is a large group of polymeric substances of different chemical nature, the sources of which are plant products. The composition of nutrients with a fibrous structure includes large quantities of second-order polysaccharides. Dietary fiber is an active participant in the digestive process, a source of essential nutrients; its deficiency or absence can lead to disruption of homeostasis, the dynamic constancy of the internal environment of the body and pathology. Dietary fiber plays an important role in the functioning of a number of organs and systems of the body and, first of all, affects the function of the colon. They adsorb significant amounts of bile acids, as well as other metabolites, toxins and electrolytes, which helps detoxify the body. For a long time, dietary fiber was considered unnecessary ballast, from which attempts were made to free products to increase their nutritional value. In this regard, a number of refined products have been developed that are completely free of ballast substances. Dietary fiber plays an extremely important role in the functioning of the colon. One of their main properties is the ability to retain water. Water-soluble dietary fibers contained in vegetables and fruits are the most hygroscopic. The ability of dietary fiber to retain water accelerates intestinal transit and peristalsis of the colon, changes intraintestinal pressure, and the concentration of fecal electrolytes. One aspect of the physiological effect of dietary fiber is its effect on mineral metabolism. There is evidence that high dietary fiber intake may disrupt the body's mineral balance. These processes are based on the metabolic properties of ballast substances, which promotes the removal of heavy metal ions, such as lead, strontium, and allows us to consider the possibility of using fiber to remove radionuclides from the body. The use of plant additives from cereals in the development of fermented milk products is of scientific interest and is one of the current trends in the development of functional nutrition products.

Effect of The Addition of Unripe Berlin Banana Flour on The Organoleptic Characteristics of Synbiotic Yogurt

Synbiotic yogurt is a fermented milk product that combines probiotic bacteria with ingredients containing prebiotic components. Unripe banana flour (UBF) contains resistant starch (RS), which contributes to the development of prebiotics in synbiotic yogurt products. The research aimed to know the effect of the addition of UBF on the organoleptic characteristics of synbiotic yogurt. The research was carried out with an unripe Berlin banana. Four treatment groups included 2% inulin (P0), 1% UBF (P1), 2% UBF (P2), and 3% UBF (P3). The organoleptic characteristics include hedonic and hedonic quality tests using 48 semi-trained panelists. The data was statistically analyzed using the Kruskall-Wallis test followed by the Mann-Whitney test. The results of the hedonic test indicate that texture, aroma, and color significantly differed between groups (P&lt;0.05), while taste did not significantly differ between groups (P&gt;0.05). The hedonic quality test indicated that there were significant differences between groups in terms of texture, aroma, and color (P&lt;0.05). However, there was no significant difference in taste between the groups (P&gt;0.05). In conclusion, formula 2% UBF (P1) is the most preferable formula based on the organoleptic test.

INVESTIGATION OF THERMOPHILIC MICROFLORA FOR THE SELECTION OF STARTER CULTURE COMPOSITION FOR A FUNCTIONAL PRODUCT

Fermented dairy products have valuable nutrients and contain a large number of living cells of lactic acid bacteria strains of lactic acid bacteria. The selection of microorganisms according to their technological and microbiological properties and their ability to develop in a dairy base, as well as organoleptic and rheological parameters of the finished fermented milk product, is an important stage in the development of new types of products. Therefore, special attention was paid to the selection of starter cultures for the production of a functional product. The authors conducted research on the selection of starter culture microflora for the production of functional products. The microbiological and organoleptic properties of selected microorganisms have been studied. When selecting microorganisms, the intensity and direction of the microbiological and biochemical processes occurring during fermentation were taken into account, which is determined by the development of the starter microflora. Thermophilic starter culture by species Lactobacillus acidophilus, Streptococcus thermophiles, Lactobacillus bulgaricus was used in the work.

Development of technology for obtaining an enriched fermented milk product based on the results of studies of the composition of the initial and final materials

The article presents the results of research on the study of the chemical, mineral and amino acid composition of the initial and final materials of the production technology of a fermented milk product enriched with an extract of vegetable raw materials. As a sample of the initial product, the optimal composition of a combination of two types of dairy raw materials, 15% mare's milk and 85% cow's milk, obtained during experiments, was selected. The final product is a fermented milk product obtained from combined dairy raw materials. It was found that in the combined dairy raw materials, the mass fraction of protein is 3.86%, the mass fraction of fat is 4.66%, the mass fraction of carbohydrates is 4.95%, in the fermented milk product, the mass fraction of protein is 4.04%, the mass fraction of fat is 4.85%, the mass fraction of carbohydrates is 6.05%. The energy value of the fermented milk product, in kcal -82.5, of the combined feedstock -75.94 kcal. The amino acid composition in the studied samples showed the presence of such components as – arginine, lysine, tyrosine, phenylalanine, histidine, leucine+isoleucine, methionine, valine, proline, threonyl, serine, alanine and glycine. The mineral composition of the combined dairy raw materials in weight% is 14.15 calcium, 15.41 potassium, 12.36 phosphorus, 1.38 magnesium and 7.34 sodium. The results of the analyses confirmed the nutritional and biological value of both the initial and final products, the technology being developed for the production of functional dairy products.

Bio-fermented milk product based on &lt;i&gt;L. acidophilus&lt;/i&gt; fortified by &lt;i&gt;Sanguisorba officinalis&lt;/i&gt; extract

The development of new functional food products containing probiotics and prebiotics of plant origin has actual scientific and practical values. The presented study purpose is the creation of fermented milk products based on L.acidophilus with the introduction of an extract of the Sanguisorba officinalis or great burnet as a prebiotic. Extracts from the great burnet rhizomes and roots have an antimicrobial activity, specifically against the bacteria Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus with pronounced bactericidal effect on various microbes of the dysenteric and paratyphoid groups, a detrimental effect on Trichomonas, fungi of the genus Candida and Giardia. Physicochemical and microbiological indicators during ripening and storage of the obtained fermented milk products based on L.acidophilus with great burnet extracts are considered. Titratable acidity, water-holding capacity, the number of lactobacilli, viscosity, and organoleptic values of fermented milk products are analyzed. It is established that the product with great burnet dry extract at a concentration of 10^-3 g/cm^3 has maximum scores for organoleptic, physicochemical, and microbiological indicators. The developed bio-fermented milk product enriched by antioxidants from extracts of biologically active compounds of great burnet will meet the national and international consumers’ demands with recommendations for therapeutic and preventive nutrition and a healthy lifestyle.

The effect of antibiotics contained in milk on the quality and safety of dairy products

During storage, the organic substances of milk undergo a number of complex changes in composition and properties, and new chemical compounds are also formed that radically change the nutritional value, normal appearance, taste and smell of the product under the influence of a number of factors. factors. Regulation of biochemical and microbiological processes during storage and processing of milk is a current trend in the dairy industry. The purpose of the experiment is to determine the effect of antibiotics on the chemical composition, microbiological parameters and organoleptic properties of raw milk and fermented milk products. During the research, it was found that with increasing concentration of penicillin, the consistency of yogurt becomes liquid and heterogeneous, with an off taste and odor. The number of lactic acid bacteria was 3.1 × 104 CFU/ml with the maximum amount of antibiotics in the product (0.1 mg/kg) and 3.4 × 108 CFU/ml without it. Statistically significant differences in the organoleptic properties of yogurt were obtained at the highest concentration of chloramphenicol (0.1 mg/kg). In the remaining samples studied, antibiotic concentrations did not affect the consistency, taste and smell of the product.

Ensuring the safety in the production of fermented milk products with enterosorbing dietary fibers

The current trend of health-conscious consumers and healthy eating habits is encouraging researchers to explore the development of food products with synbiotic benefits. Dietary fiber in vegetables, fruits and cereals is one of the promising prebiotics and its use in supplementing dairy product formulations. However, the important point is to produce safe products according to the current standards of the country of production and sale. This article identifies critical control points and conducts a metrological study of quality control at each stage in the production of a new fermented milk product with the addition of enterosorbing dietary fibers. The study identified potential and five critical control points and presented an optimized scheme with factors that ensure the safety and quality of the final product.

Starch Enzyme Modification Optimization for the Texture Correction of a Low-Fat Fermented Milk Product

A fermented milk product with low or zero fat content is in demand among consumers. In this regard, an urgent task for manufacturers is to preserve or imitate the rheological and textural parameters of a full-fat product that affect the organoleptic evaluation and the overall attractiveness of a low-fat product for the buyer. The authors studied an impact of tapioca starch treated with the industrial amylolytic enzyme preparation Alfalad BN® as a texture corrector in the low-fat thermostatic product technology. The methods included determination of moisture retention, syneresis, dynamic viscosity, and texture profile analysis. A man developed the optimization model for the starch enzyme modification for correcting the texture of a low-fat fermented milk product based on a symbiotic starter culture. Using mathematical modeling (RSM response surface methodology), the researchers found the optimal range of enzyme preparation concentration values (0.20–0.25 U/g) and time (25–35 min) for starch modification to apply in the fermented milk drink technology further. Developed experimental fermented milk product has the best textural and rheological indicators in comparison with the control – a low-fat product using traditional technology: syneresis of the product with starch is lower by 21.56 %, viscosity is higher by 27.09 %, adhesion is higher by 7.49 %, stickiness is lower by 6.15 %, CWS is higher by 4.61 %; other parameters are at the level similar to the control. The described changes lead to an increase in the consistency and density of the low-fat fermented milk product improving the consumer perception of its taste.

Potensi Susu Fermentasi dalam Mempengaruhi Parameter

This study aims to explain the effect of milk on hematological parameters. The method used is a literature review made through a systematic approach, using the PICO method, by searching for articles in the Pubmed, Scopus, and EBSCOhost databases. The research results showed that 425 articles were found, and seven articles met the inclusion criteria; these articles were fermented milk containing lactic acid bacteria, which are probiotics. Fermented milk products such as yogurt, Yakult, kefir, active, fermented goat's milk, and dahi influence hematological parameters. Namely, they can increase hemoglobin, ferritin, and erythrocyte count and reduce MCV. Milk fermentation affects hematological parameters, namely increasing hemoglobin, ferritin, and erythrocyte count and reducing MCV. Keywords: Anemia, Probiotics, Fe Absorption, Fermented Milk, Iron Status

ИССЛЕДОВАНИЕ КАЧЕСТВА ТВОРОЖНОЙ МАССЫ, ОБОГАЩЕННОЙ РАСТИТЕЛЬНЫМ СЫРЬЕМ

The purpose of research is to study the quality indicators of a fermented milk product – curd mass, in the production of which local raw materials are used - dried rose hips of the Desertnyj variety. Objectives: to study the quality of manufactured samples of curd mass enriched with plant raw materials in comparison with the control; to determine the organoleptic and bacteriological characteristics of all samples; to study the physico-chemical indicators of the curd mass and calculate the economic efficiency of the research carried out. The experimental part of the work was carried out at the Krasnoyarsk State Agrarian University. 4 samples of curd mass were studied, the control was produced according to the generally accepted technology GOST 31680-2012, in experimental 1, 2 % of the main raw material was replaced with crushed rose hips, in experimental samples 2 and 3 – by 4 and 6 %, respectively. In the studied samples, the curd mass had a dense consistency, the color in the control and experimental sample 1 did not change, in experimental 2 and 3 it acquired a pinkish tint. With an increase in the amount of crushed rose hips in the product recipe, all test samples acquired a sweet and sour taste, most pronounced in test sample 3. The results of physical and chemical studies showed a decrease of 0.7 % in the mass fraction of protein in sample 3 and moisture in samples 2 and 3 by 1 %, the mass fraction of fat in sample 3 decreased by 2 % compared to the control sample. The mass fraction of sucrose increased in all test samples, in samples 2 and 3 – by 2.8 %, in test sample 1 – by 2 %. The total acidity and temperature of the product upon release from the plant remained unchanged in all samples. Enrichment of the curd mass with crushed rose hips did not affect the microbiological parameters of the studied samples, and analysis of data on the economic efficiency of production indicates a higher profitability of curd mass production with the replacement of 4 % of the main raw material with rose hips.

Isolation and assessment of novel exopolysaccharide-producing Weissella confusa ABRIIFBI-96 isolated from an Iranian homemade dairy fermented food “Tof” as a main starter culture for probiotic fermented milk

This study focused on evaluating lactic acid bacteria (LAB) isolates for their probiotic characteristics and safety, as well as potential as a primary starter culture in probiotic fermented milk production. Ten LAB isolates underwent in vitro testing to assess their probiotic potential. While these isolates were susceptible to ten antibiotics, none of them showed sensitivity to vancomycin. Particularly, the isolates, especially ABRIIFBI-96, demonstrated substantial resistance to bile salts (80.48 ± 0.37%) and low pH (82.05 ± 0.1%), along with strong adhesion ability to Caco-2 cells (22.59 ± 0.36%). Notably, isolate ABRIIFBI-96 exhibited impressive auto-aggregation and co-aggregation capabilities (33.45 ± 0.36 and 28.66 ± 0.4, respectively) and had the highest cholesterol-reducing capability (35.63 ± 0.64%) without any hemolytic activity. Furthermore, isolate ABRIIFBI-96 demonstrated robust bile salt hydrolase activity with both glycolic and taurocholic tested bile salts. It exhibited inhibitory activity against all tested pathogens, establishing its significant probiotic potential and suitability for incorporation into functional food formulations. The results were highly promising, as the use of isolate ABRIIFBI-96 reduced fermentation time to just 10 h, and the microbial count in the final products ensured their probiotic activity even after 28 days of storage (with a reduction of 0.899 log CFU mL−1).