Dimethylsulfide Production Research Articles

Microplastics and copper impacts on feeding, oxidative stress, antioxidant enzyme activity, and dimethylated sulfur compounds production in Manila clam Ruditapes philippinarum

Microplastics (MPs) are widespread ocean pollutants and many studies have explored their effects. However, research on MPs combined impact with copper (Cu) on dimethylated sulfur compound production is limited. Dimethyl sulfide (DMS) is an important biogenic sulfur compound related to global temperatures. This study examined the ecotoxicological effects of polyamide 6 MPs and Cu on dimethylsulfoniopropionate (DMSP), DMS, and dimethyl sulfoxide (DMSO) production in Manila clams (Ruditapes philippinarum). Our findings showed that MPs and Cu increased oxidative stress, indicated by higher superoxide anion radical production and malondialdehyde levels while decreasing glutathione contents and increasing superoxide dismutase activities. Additionally, MPs and Cu exposure reduced DMS and dissolved DMSO (DMSOd) concentrations due to decreased grazing. These results contribute to a better understanding of the ecotoxicological effects of MPs/Cu on bivalves and their roles in the organic sulfur cycle, suggesting a need for further research on long-term impacts on them.

Abiotic Production of Dimethyl Sulfide, Carbonyl Sulfide, and Other Organosulfur Gases via Photochemistry: Implications for Biosignatures and Metabolic Potential

Among the atmospheric gases that have been proposed as possible biosignatures in exoplanetary atmospheres, organosulfur gases are currently considered one of the more robust indicators of extant life. These gases include dimethyl sulfide (DMS; CH3SCH3), carbonyl sulfide (OCS), and carbon disulfide (CS2), which are predominantly secondary metabolic products of living organisms on Earth. Here we present results that challenge this interpretation and provide constraints on the robustness of organosulfur gases as biosignatures. Through laboratory photochemical experiments, we show the abiotic production of organosulfur gases, including DMS, OCS, methane thiol (CH3SH), ethane thiol (C2H5SH), CS2, and ethyl methyl sulfide (CH3CH2SCH3) via photochemistry in analog atmospheres. Gas-phase products of H2S/CH4/N2 haze photochemistry, with or without CO2, were collected and analyzed using gas chromatography equipped with sulfur chemiluminescence detection. Depending on the starting conditions, we estimate that DMS, OCS, CH3SH, CH3CH2SH, CS2, and CH3CH2SCH3 are produced in mixing ratios >10−1 ppmv. We further demonstrate that as the mixing ratio of CO2 increases, so does the relative importance of OCS compared to DMS. Although our results constrain the robustness of common organosulfur gases as biosignatures, the presence of these compounds may serve as an indicator of metabolic potential on exoplanets.

Size-dependent influences of nano- and micro-plastics exposure on feeding, antioxidant systems, and organic sulfur compounds in ciliate Uronema marinum

Protozoa play a pivotal role in the microbial cycle, and ciliated protozoan grazing habits are associated with dimethyl sulfide (DMS) cycle. Many studies have explored the impacts of nanoplastics (NPs) and microplastics (MPs) on ecotoxicological effects of ciliates. However, limited research exists on NPs and MPs influences on the production of organic sulfur compounds. The impact of NPs and MPs on the production of dimethyl sulfoxide (DMSO) and carbonyl sulfide (COS) remains unclear. Therefore, we examined the impacts of three concentrations (1 × 105, 5 × 105, and 1 × 106 items/mL) of polystyrene (PS) NPs (50 nm) and MPs (1 and 5 μm) on the ecotoxicology and DMS/dimethylsulfoniopropionate (DMSP)/DMSO/COS production in the ciliate Uronema marinum. NPs and MPs exposure were found to reduce the abundance, growth rate, volume, and biomass of U. marinum. Additionally, NPs and MPs increased the superoxide anion radical (O2˙─) production rates and malondialdehyde (MDA) contents (24 h), leading to a decline in glutathione (GSH) content and an ascend in superoxide dismutase (SOD) activity to mitigate the effects of reactive oxygen species (ROS). Exposure to PS NPs and MPs decreased the ingestion rates of algae by 7.5–14.4%, resulting in decreases in DMS production by 56.8–85.4%, with no significant impact on DMSO production. The results suggest a distinct pathway for the production of DMSO or COS compared to DMS. These findings help us to understand the NPs and MPs impacts on the marine ecosystem and organic sulfur compound yield, potentially influencing the global climate.

Factors Controlling DMS Emission and Atmospheric Sulfate Aerosols in the Western Pacific Continental Sea

AbstractThe western Pacific continental sea significantly influences the regulation of climate‐active gases budget and the burden of sulfate aerosols. An underway shipboard measurement device was used to determine the dimethyl sulfide (DMS) in the surface seawater and overlying atmosphere in the East China Continental Sea. The average concentration of DMS in atmosphere and seawater was 122.8 ± 86.2pptv and 6.47 ± 3.58 nmol L−1, respectively. The variation trend of surface water DMS in the western Pacific continental sea was influenced by the abundance and composition of phytoplankton under different ocean current systems, with a significant impact from eddies on DMS production in the South China Sea. By eliminating the influence of terrestrial sources and limiting air mass transport within the marine boundary layer, strong correlations were established between atmospheric DMS and air mass exposure to chlorophyll (Echl), as well as between aerosol methanesulfonic acid (MSA) and Echl. During the aerosol sampling period, the atmospheric DMS levels (1,057 ± 371 ng/m3) were significantly higher than MSA levels (46.3 ± 59.8 ng/m3) in the East China Sea, where the conversion of DMS to MSA was not affected by changes in DMS concentration. The sea‐to‐air fluxes of DMS varied over a wide range, from 0.02 to 156.0 μmol m−2 d−1, with an average of 14.35 ± 18.58 μmol m−2 d−1. Marine DMS emissions play a critical role in the formation of sulfur aerosols on the western Pacific continental shelf, accounting for 24.6 ± 7.6% (14.8%–37.8%) of the total sulfate aerosols.

Characterizing spatio-temporal variations of dimethyl sulfide in the Yellow and East China Sea based on BP neural network

Dimethyl sulfide (DMS), an organic volatile sulfide produced from Dimethylsulfoniopropionate (DMSP), exerts a significant impact on the global climate change. Utilizing published literature data spanning from 2005 to 2020, a BP neural network (BPNN) model of the surface seawater DMS in the Yellow and East China Sea (YECS) was developed to elucidate the influence of various marine factors on the DMS cycle. Results indicated that the six parameters inputted BPNN model, that include the time (month), latitude and longitude, sea-surface chlorophyll a (Chl-a), sea-surface temperature (SST), and sea-surface salinity (SSS), yielded the optimized simulation results (R2 = 0.71). The optimized estimation of surface seawater DMS in the YECS were proved to be closely aligned with the observed data across all seasons, which demonstrated the model’s robust applicability. DMS concentration in surface seawater were found to be affected by multiple factors such as Chl-a and SST. Comparative analysis of the three environmental parameters revealed that Chl-a exhibited the most significant correlation with surface seawater DMS concentration in the YECS (R2 = 0.20). This underscores the pivotal role of chlorophyll in phytoplankton photosynthesis and DMS production, emphasizing its importance as a non-negligible factor in the study of DMS and its sulfur derivatives. Furthermore, surface seawater DMS concentration in the YECS exhibited positive correlations with Chl-a and SST, while displaying a negative correlation with SSS. The DMS concentration in the YECS show substantial seasonal variations, with the maximum value (5.69 nmol/L) in summer followed in decreasing order by spring (3.96 nmol/L), autumn (3.18 nmol/L), and winter (1.60 nmol/L). In the YECS, there was a gradual decrease of DMS concentration from the nearshore to the offshore, especially with the highest DMS concentration concentrated in the Yangtze River Estuary Basin and the south-central coastal part off the Zhejiang Province. Apart from being largely composed by the release of large amounts of nutrients from anthropogenic activities and changes in ocean temperature, the spatial and temporal variability of DMS may be driven by additional physicochemical parameters.

Novel insights into dimethylsulfoniopropionate cleavage by deep subseafloor fungi

Dimethylsulfoniopropionate (DMSP), a key organic sulfur compound in marine and subseafloor sediments, is degraded by phytoplankton and bacteria, resulting in the release of the climate-active volatile gas dimethylsulfide (DMS). However, it remains unclear if dominant eukaryotic fungi in subseafloor sediments possess specific abilities and metabolic mechanisms for DMSP degradation and DMS formation. Our study provides the first evidence that fungi from coal-bearing sediments ∼2 km below the seafloor, such as Aspergillus spp., Chaetomium globosum, Cladosporium sphaerospermum, and Penicillium funiculosum, can degrade DMSP and produce DMS. In Aspergillus sydowii 29R-4-F02, which exhibited the highest DMSP-dependent DMS production rate (16.95 pmol/μg protein/min), two DMSP lyase genes, dddP and dddW, were identified. Remarkably, the dddW gene, previously observed only in bacteria, was found to be crucial for fungal DMSP cleavage. These findings not only extend the list of fungi capable of degrading DMSP, but also enhance our understanding of DMSP lyase diversity and the role of fungi in DMSP decomposition in subseafloor sedimentary ecosystems.

Effect of removing bacteria and dissolved organic matter on DMS production in Nannochloropsis oceanica concentrates

The aim of this study was to determine the influence of bacteria and organic matter on the occurrence of dimethyl sulfide (DMS) over the shelf life of Nannochloropsis oceanica concentrates. Two concentrates were compared: Control treatment, composed of biomass collected by centrifugation and diluted to a cell density of 5 × 109 cells·mL−1, and a washed concentrate treatment, where the biomass underwent successive centrifugations to remove dissolved organic matter (DOM) and bacteria before being diluted to a cell density of 5 × 109 cells·mL−1. Both concentrates were stored at two temperatures: 23 °C for 10 days and 4 °C for 85 days. Cellular viability, bacterial density, DOM, and DMS concentration were assessed. The washing process was responsible for removing approximately 84 % of the bacterial load from the concentrates. This significant reduction played a crucial role in decreasing the concentrations of DOM and DMS over the storage period. Furthermore, the process did not impair the cellular viability of microalgae. The findings underscore the importance of carefully managing bacterial and organic components in microalgal concentrates, especially when considering the preservation of freshness and cellular viability, which are key factors in applications such as larviculture.

Effects of micro- and nano-plastics on growth, antioxidant system, DMS, and DMSP production in Emiliania huxleyi

Due to the potential impacts of microplastics (MPs) and nanoplastics (NPs) on algal growth and thereby affect the climate-relevant substances, dimethylsulfoniopropionate (DMSP) and dimethyl sulfide (DMS), we studied the polystyrene (PS) MPs and NPs of 1 μm and 80 nm impacts on the growth, chlorophyll content, reactive oxygen species (ROS), antioxidant enzyme activity, and DMS/DMSP production in Emiliania huxleyi. E. huxleyi is a prominent oceanic alga that plays a key role in DMS and DMSP production. The results revealed that high concentrations of MPs and NPs inhibited the growth, carotenoid (Car), and Chl a concentrations of E. huxleyi. However, short-time exposure to low concentrations of PS MPs and NPs stimulated the growth of E. huxleyi. Furthermore, high concentrations of MPs and NPs resulted in an increase in the superoxide anion radical (O2.–) production rate and a decrease in the malondialdehyde (MDA) content compared with the low concentrations. Exposure to MPs and NPs at 5 mg L−1 induced superoxide dismutase (SOD) activity as a response to scavenging ROS. High concentrations of MPs and NPs significantly inhibited the production of DMSP and DMS. The findings of this study support the potential ecotoxicological impacts of MPs and NPs on algal growth, antioxidant system, and dimethylated sulfur compounds production, which maybe potentially impact the global climate.

Plastic Litter Emits the Foraging Infochemical Dimethyl Sulfide after Submersion in Freshwater Rivers.

Plastic pollution is widespread throughout aquatic environments globally, with many organisms known to interact with and ingest plastic. In marine environments, microbial biofilms that form on plastic surfaces can produce the odorous compound dimethyl sulfide (DMS), which is a known foraging cue. This has been shown to increase the ingestion of plastic by some invertebrates and therefore act as a biological factor which influences the risks of plastic to marine ecosystems. In freshwater however, the production of DMS has been largely overlooked, despite the known sensitivity of some freshwater species to this compound. To address this gap, the present study analyzed the production of DMS by biofilms which formed on low-density polyethylene and polylactic acid films after 3 and 6 weeks of submersion in either a rural or an urban United Kingdom river. Using gas chromatography-mass spectrometry, the production of DMS by these biofilms was consistently identified. The amount of DMS produced varied significantly across river locations and materials, with surfaces in the urban river generally producing a stronger signal and plastics producing up to seven times more DMS than glass control surfaces. Analysis of biofilm weight and photosynthetic pigment content indicated differences in biofilm composition across conditions and suggested that DMS production was largely driven by nonphotosynthetic taxa. For the first time this work has documented the production of DMS by plastic litter after submersion in freshwater rivers. Further work is now needed to determine if, as seen in marine systems, this production of DMS can encourage the interaction of freshwater organisms with plastic litter and therefore operate as a biological risk factor in the impacts of plastic on freshwater environments. Environ Toxicol Chem 2024;43:1485-1496. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Changes in global DMS production driven by increased CO2 levels and its impact on radiative forcing

Our study highlights the importance of understanding the future changes in dimethyl-sulfide (DMS), the largest natural sulfur source, in the context of ocean acidification driven by elevated CO2 levels. We found a strong negative correlation (R2 = 0.89) between the partial pressure of carbon dioxide (pCO2) and sea-surface DMS concentrations based on global observational datasets, not adequately captured by the Coupled Model Intercomparison Project Phase 6 (CMIP6) Earth System Models (ESMs). Using this relationship, we refined projections of future sea-surface DMS concentrations in CMIP6 ESMs. Our study reveals a decrease in global sea-surface DMS concentrations and the associated aerosol radiative forcing compared to ESMs’ results. These reductions represent ~9.5% and 11.1% of the radiative forcings resulting from aerosol radiation and cloud interactions in 2100 reported by the Intergovernmental Panel on Climate Change Sixth Assessment Report. Thus, future climate projections should account for the climate implications of changes in DMS production due to ocean acidification.

Effects of nanoplastics exposure on ingestion, life history traits, and dimethyl sulfide production in rotifer Brachionus plicatilis

Microplastics (MPs) and nanoplastics (NPs) have gained global concern due to their detrimental effects on marine organisms. We investigated the effects of 80 nm polystyrene (PS) NPs on life history traits, ingestion, and dimethyl sulfide (DMS) and dimethylsulfoniopropionate (DMSP) production in the rotifer Brachionus plicatilis. Fluorescently labeled 80 nm PS NPs were ingested by the rotifer B. plicatilis and accumulated in the digestive tract. The lethal rates of B. plicatilis exposed to NPs were dose-dependent. High concentrations of PS NPs exposure had negative effects on developmental duration, leading to prolonged embryonic development and pre-reproductive periods, shortened reproductive period, post-reproductive period, and lifespan in B. plicatilis. High concentrations of PS NPs exposure inhibited life table demographic parameters such as age-specific survivorship and fecundity, generation time, net reproductive rate, and life expectancy. Consequently, the population of B. plicatilis was adversely impacted. Furthermore, exposure to PS NPs resulted in a reduced ingestion rate in B. plicatilis, as well as a decreased in DMS, particulate DMSP (DMSPp) concentration, and DMSP lyase activity (DLA), which exhibited a dose-response relationship. B. plicatilis grazing promoted DLA and therefore increased DMS production. PS NPs exposure caused a decline in the increased DMS induced by rotifer grazing. Our results help to understand the ecotoxicity of NPs on rotifer and their impact on the biogeochemical cycle of dimethylated sulfur compounds.

Quorum Sensing Regulates the Production of Methanethiol in Vibrio harveyi.

Methanethiol (MeSH) and dimethyl sulfide (DMS) are important volatile organic sulfur compounds involved in atmospheric chemistry and climate regulation. However, little is known about the metabolism of these compounds in the ubiquitous marine vibrios. Here, we investigated MeSH/DMS production and whether these processes were regulated by quorum-sensing (QS) systems in Vibrio harveyi BB120. V. harveyi BB120 exhibited strong MeSH production from methionine (Met) (465 nmol mg total protein-1) and weak DMS production from dimethylsulfoniopropionate (DMSP) cleavage. The homologs of MegL responsible for MeSH production from L-Met widely existed in vibrio genomes. Using BB120 and its nine QS mutants, we found that the MeSH production was regulated by HAI-1, AI-2 and CAI-1 QS pathways, as well as the luxO gene located in the center of this QS cascade. The regulation role of HAI-1 and AI-2 QS systems in MeSH production was further confirmed by applying quorum-quenching enzyme MomL and exogenous autoinducer AI-2. By contrast, the DMS production from DMSP cleavage showed no significant difference between BB120 and its QS mutants. Such QS-regulated MeSH production may help to remove excess Met that can be harmful for vibrio growth. These results emphasize the importance of QS systems and the MeSH production process in vibrios.

The production of dimethylsulfoniopropionate by bacteria with mmtN linked to non-ribosomal peptide synthase gene

ABSTRACT Dimethylsulfoniopropionate (DMSP) is a vital sulfur-containing compound with worldwide significance, serving as the primary precursor for dimethyl sulfide (DMS), a volatile sulfur compound that plays a role in atmospheric chemistry and influences the Earth’s climate on a global scale. The study investigated the ability of four bacterial strains, namely Acidimangrovimonas sediminis MS2-2 (MS2-2), Hartmannibacter diazotrophicus E18T (E18T), Rhizobium lusitanum 22705 (22705), and Nitrospirillum iridis DSM22198 (DSM22198), to produce and degrade DMSP. These strains were assessed for their DMSP synthesis ability with the mmtN linked to non-ribosomal peptide synthase (NRPS) gene. The results showed that MS2-2, and E18T bacteria, which contained the mmtN but not linked to an NRPS gene, increased DMSP production with increasing salinity. The highest production of DMSP was achieved at 25 PSU when either methionine was added or low nitrogen conditions were present, yielding 1656.03 ± 41.04 and 265.59 ± 9.17 nmol/mg protein, respectively, and subsequently under the conditions of methionine addition or low nitrogen, both strains reached their maximum DMSP production at 25 PSU. Furthermore, the strains MS2-2, E18T, and 22705 with the mmtN gene but not linked to an NRPS gene were found to be involved in DMS production. This research contributes to the understanding of the genes involved in DMSP biosynthesis in bacteria that produce DMSP.

Gephyrocapsa huxleyi (Emiliania huxleyi) as a model system for coccolithophore biology.

Coccolithophores are the most abundant calcifying organisms in modern oceans and are important primary producers in many marine ecosystems. Their ability to generate a cellular covering of calcium carbonate plates (coccoliths) plays a major role in marine biogeochemistry and the global carbon cycle. Coccolithophores also play an important role in sulfur cycling through the production of the climate-active gas dimethyl sulfide. The primary model organism for coccolithophore research is Emiliania huxleyi, now named Gephyrocapsa huxleyi. G. huxleyi has a cosmopolitan distribution, occupying coastal and oceanic environments across the globe, and is the most abundant coccolithophore in modern oceans. Research in G. huxleyi has identified many aspects of coccolithophore biology, from cell biology to ecological interactions. In this perspective, we summarize the key advances made using G. huxleyi and examine the emerging tools for research in this model organism. We discuss the key steps that need to be taken by the research community to advance G. huxleyi as a model organism and the suitability of other species as models for specific aspects of coccolithophore biology.

Measurement report: Carbonyl sulfide production during dimethyl sulfide oxidation in the atmospheric simulation chamber SAPHIR

Abstract. Carbonyl sulfide (OCS), the most abundant sulfur gas in the Earth's atmosphere, is a greenhouse gas, a precursor to stratospheric sulfate aerosol, and a proxy for terrestrial CO2 uptake. Estimates of important OCS sources and sinks still have significant uncertainties and the global budget is not considered closed. One particularly uncertain source term, the OCS production during the atmospheric oxidation of dimethyl sulfide (DMS) emitted by the oceans, is addressed by a series of experiments in the atmospheric simulation chamber SAPHIR in conditions comparable to the remote marine atmosphere. DMS oxidation was initiated with OH and/or Cl radicals and DMS, OCS, and several oxidation products and intermediates were measured, including hydroperoxymethyl thioformate (HPMTF), which was recently found to play a key role in DMS oxidation in the marine atmosphere. One important finding is that the onset of HPMTF and OCS formation occurred faster than expected from the current chemical mechanisms. In agreement with other recent studies, OCS yields between 9 % and 12 % were observed in our experiments. Such yields are substantially higher than the 0.7 % yield measured in laboratory experiments in the 1990s, which is generally used to estimate the indirect OCS source from DMS in global budget estimates. However, we do not expect the higher yields found in our experiments to directly translate into a substantially higher OCS source from DMS oxidation in the real atmosphere, where conditions are highly variable, and, as pointed out in recent work, heterogeneous HPMTF loss is expected to effectively limit OCS production via this pathway. Together with other experimental studies, our results will be helpful to further elucidate the DMS oxidation chemical mechanism and in particular the paths leading to OCS formation.

Effects of coastal shellfish farming on dimethylsulfide production

Dimethylsulfide (DMS) plays an important role in the sulfur biogeochemical cycle and cloud formation on Earth. In the past few decades, coastal shellfish breeding facilities have developed rapidly in shallow coastal regions. However, the effects of coastal shellfish farming on DMS production remain controversial at present. The Yantai offshore is an important shellfish farming region in northern China and the effect of shellfish farming on DMS production was explored combining field investigation and laboratory cultivation in this study. The results showed that coastal shellfish farming can promote the conversion of DMSP to DMS, especially in the shellfish rapid growth period and harvest period. However, different from the zooplankton “sloppy feeding”, the shellfish “filter-feeding” first repackages the phytoplanktonic DMSP into fecal DMSP, and then degrades DMSPp into DMS by attracting attached bacteria. Throughout the entire shellfish farming period, average DMS flux in shellfish region was 1.37 μmol m−2 d−1 higher than that in non-shellfish region. Based on the investigation results Yantai coastal shellfish farming region, it can be estimated that the increment of DMS caused by Chinese coastal shellfish farming can reach 2.96 × 106 mol per year.

Dimethylated Sulfur, Methane and Aerobic Methane Production in the Yellow Sea and Bohai Sea

AbstractThis paper presents a comprehensive study of biogenic dimethylated sulfur compounds and methane (CH4) from 27 March to 16 April 2018 (spring) and from 24 July to 10 August 2018 (summer) in the Yellow Sea and Bohai Sea. The overall distributions of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP, precursor of DMS), dimethylsulfoxide (DMSO, oxidation product of DMS) and CH4 in surface waters were characterized by elevated concentrations in summer and in coastal waters, coupled to phytoplankton biomass and terrestrially sourced inputs. Surface waters were oversaturated with CH4. The flux distributions of DMS and CH4 were generally consistent with their concentration distributions. In situ incubation experiments revealed that microbial consumption was the main removal mechanism for DMS, which could remove up to 72.1% of the total DMS compared to sea‐to‐air exchange in the surface layer. High DMS release and enhanced DMS yields (34%–62%) suggested more obvious influence from high DMSP concentrations (1 or 5 μm L−1) on DMS production in oligotrophic waters with lower bacterial sulfur demand in contrast to near‐shore waters. Positive correlations were found between CH4 and DMSP (dissolved) in summer and DMSO (particulate and dissolved) in spring. A DMSP addition experiment suggested that DMSP could act as a precursor for aerobic methanogenesis, and CH4 preferentially occurs under nitrogen‐stressed conditions in the surface layers of the Yellow Sea and Bohai Sea.

Microbial Dimethylsulfoniopropionate Cycling in Deep Sediment of the Mariana Trench.

Dimethylsulfoniopropionate (DMSP) and related organic sulfur compounds play key roles in global sulfur cycling. Bacteria have been found to be important DMSP producers in seawater and surface sediments of the aphotic Mariana Trench (MT). However, detailed bacterial DMSP cycling in the Mariana Trench subseafloor remains largely unknown. Here, the bacterial DMSP-cycling potential in a Mariana Trench sediment core (7.5 m in length) obtained at a 10,816-m water depth was investigated using culture-dependent and -independent methods. The DMSP content fluctuated along the sediment depth and reached the highest concentration at 15 to 18 cm below the seafloor (cmbsf). dsyB was the dominant known DMSP synthetic gene, existing in 0.36 to 1.19% of the bacteria, and was identified in the metagenome-assembled genomes (MAGs) of previously unknown bacterial DMSP synthetic groups such as Acidimicrobiia, Phycisphaerae, and Hydrogenedentia. dddP, dmdA, and dddX were the major DMSP catabolic genes. The DMSP catabolic activities of DddP and DddX retrieved from Anaerolineales MAGs were confirmed by heterologous expression, indicating that such anaerobic bacteria might participate in DMSP catabolism. Moreover, genes involved in methanethiol (MeSH) production from methylmercaptopropionate (MMPA) and dimethyl sulfide (DMS), MeSH oxidation, and DMS production were highly abundant, suggesting active conversions between different organic sulfur compounds. Finally, most culturable DMSP synthetic and catabolic isolates possessed no known DMSP synthetic and catabolic genes, and actinomycetes could be important groups involved in both DMSP synthesis and catabolism in Mariana Trench sediment. This study extends the current understanding of DMSP cycling in Mariana Trench sediment and highlights the need to uncover novel DMSP metabolic genes/pathways in extreme environments. IMPORTANCE Dimethylsulfoniopropionate (DMSP) is an abundant organosulfur molecule in the ocean and is the precursor for the climate-active volatile gas dimethyl sulfide. Previous studies focused mainly on bacterial DMSP cycling in seawater, coastal sediment, and surface trench sediment samples, but DMSP metabolism in the Mariana Trench (MT) subseafloor sediments remains unknown. Here, we describe the DMSP content and metabolic bacterial groups in the subseafloor of the MT sediment. We found that the tendency for vertical variation of the DMSP content in the MT was distinct from that of the continent shelf sediment. Although dsyB and dddP were the dominant DMSP synthetic and catabolic genes in the MT sediment, respectively, both metagenomic and culture methods revealed multiple previously unknown DMSP metabolic bacterial groups, especially anaerobic bacteria and actinomycetes. The active conversion of DMSP, DMS, and methanethiol may also occur in the MT sediments. These results provide novel insights for understanding DMSP cycling in the MT.

Aerobic methylation of hydrogen sulfide to dimethylsulfide in diverse microorganisms and environments.

Dimethylsulfide (DMS) is the major biosulfur source emitted to the atmosphere with key roles in global sulfur cycling and potentially climate regulation. The main precursor of DMS is thought to be dimethylsulfoniopropionate. However, hydrogen sulfide (H2S), a widely distributed and abundant volatile in natural environments, can be methylated to DMS. The microorganisms and the enzymes that convert H2S to DMS, and their importance in global sulfur cycling were unknown. Here we demonstrate that the bacterial MddA enzyme, previously known as a methanethiol S-methyltransferase, could methylate inorganic H2S to DMS. We determine key residues involved in MddA catalysis and propose the mechanism for H2S S-methylation. These results enabled subsequent identification of functional MddA enzymes in abundant haloarchaea and a diverse range of algae, thus expanding the significance of MddA mediated H2S methylation to other domains of life. Furthermore, we provide evidence for H2S S-methylation being a detoxification strategy in microorganisms. The mddA gene was abundant in diverse environments including marine sediments, lake sediments, hydrothermal vents and soils. Thus, the significance of MddA-driven methylation of inorganic H2S to global DMS production and sulfur cycling has likely been considerably underestimated.

Responses of biogenic dimethylated sulfur compounds to environmental changes in the northwestern Pacific continental sea

AbstractContinental seas are facing rapid environmental shifts, but how biogenic dimethylated sulfur compounds, including dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), and dimethylsulfoxide (DMSO), will respond to these environmental changes remains poorly understood. Here we investigated the effects of nutrient input, ocean acidification, and dust deposition on the phytoplankton community and organic sulfur cycle in the East China Sea. Nutrient input promoted phytoplankton growth and increased the concentrations of DMS, DMSP, and DMSO. With sufficient nutrients, especially nitrate, the dissolved DMSP degradation was inhibited, and the bacterial DMSP‐cleavage pathway (inferred by dddP gene abundance) was enhanced, causing increased DMS production. The sensitivity of phytoplankton biomass and DMS to ocean acidification varied with different initial nutrient levels, demonstrating insensitivity under eutrophic conditions and negative responses under nutrient‐limited conditions. The ocean acidification promoted the dissolved DMSP degradation and bacterial DMSP‐demethylation pathway (inferred by dmdA gene abundance) and weakened the DMS production, causing the decreases of DMS and DMSP. The nutrient from dust deposition (2 mg L−1) was identified as the key factor in enhancing phytoplankton biomass and the organic sulfur compounds concentrations, but trace metals input from dust deposition had no significant effect. This study has identified environmental drivers and suppressors of phytoplankton and biogenic dimethylated sulfur compounds in a changing marine environment, which will enable the effective modeling of future climate change.