SummaryAmorphophallus albus, belonging to the family Araceae, has attracted widespread attention due to its considerable economic and medicinal importance. The natural propagation coefficient of A. albus is very low, which limits application of this crop. In vitro corms can be used for propagation of A. albus and have been proved to be superior over in vitro plantlets. To optimise procedures for in vitro corm production and multiplication, the effects of phytohormones, sucrose concentrations and incubation conditions with desirable phytohormone combinations for callus induction, corm formation and corm growth of A. albus were investigated. The results showed that calli were induced at high frequency from petiole segments on Murashige and Skoog medium supplemented with 1.0 mg l–1 naphthaleneacetic acid (NAA) and 1.0 mg l–1 6-benzyladenine (BA). Compact nodular calli were desirable for corm formation, and optimum corm formation was obtained in the presence of 0.5 mg l–1 NAA and 2.0 mg l–1 BA. With this auxin and cytokinin combination, an increase in sucrose concentration from 2% to 6% (w/v) significantly increased the corm formation rate and favoured corm growth, but negative effects occurred at higher sucrose concentrations. By incubating over a range of temperatures from 19°C – 28°C, 22°C produced the largest numbers of corms and highest mean fresh weight of each corm. Short-day (8 h) or long-day (16 h) photoperiods did not affect corm formation and growth significantly, except that corm weight fell under long-day conditions. The multiplication rate of in vitro corms was enhanced by apical meristem wounding. It was possible to store in vitro produced corms at 4°C for as long as 90 d to overcome apical dormancy and accelerate sprouting after planting into soil. This work has established an efficient protocol for multiplication of A. albus through an in vitro corm system.