Cellulase Enzyme Production Research Articles

Isolation and Celulolytic Activity Assay of Actinobacteria Isolated from Palm Oil Wastewater

Cellulose plays an important role in the carbon cycle in nature and is the largest compound. This study aimed to isolate, to characterize and to determine actinobacteria that capable of producing cellulases. The sampling method in this study was carried out by purposive sampling at the outlet point of aeration pond of the wastewater treatment plant (IPAL) from palm oil waste station of PT. Teupin Lada. Isolation of actinobacteria was carried out on Humic Acid Vitamin b Agar (HVA), morphological characterization was carried out on Yeast Malt Agar (YMA), Yeast Starch Agar (YSA), Oatmeal Agar (OA), and microscopic characterization of actinobacteria and measuring the diameter of the clear zone formed on Carboxymethyl Cellulose (CMC) medium using the indicator Congo Red. Eight isolates were obtained from the isolation. Of the 8 isolates obtained, 7 of them were able to produce cellulase enzymes which were measured based on the clear zone formed in the test Congo Red on Carboxymethil Cellulose (CMC), and one isolate did not show any clear zones. The highest value of Cellulolytic Index (IS) was obtained from isolate ATLS-05, namely 8.38 mm. HIGHLIGHTS Cellulose plays an important role in the carbon cycle in nature and is the largest compound The high waste load especially palm oil mill effluent (Elaeis guineensis) or known as Palm Oil Mill Effluent(POME) could cause various problems for the environment and society. POME is wastewater from the palm oil industry, which is one of the most polluting agro-industrial wastes Actinobacteria are one of the soil microbes which have the greatest abundance and play an important role in the decomposition process. One of the roles of soil microbes is too degrading cellulose GRAPHICAL ABSTRACT

Detection of Extracellular Enzymes Produced by Fungi Isolated from Dried Fruits

Screening of 57 fungal isolates isolated from dried fruits for the production of lipase, cellulase, invertase, and protease showed that there is a variation in enzyme production not only among the different genera and species, but also among the different isolates in the same species. Thirty two of tested fungi (66%) belonging to A. flavus, A. fumigatus, A. niger, A. terreus, A. tamarii, Cochliobolus spicifer, Humicola insolens, F. oxysporium, P. glaprum, P. oxalicum, P. stekii, P. variabile and Phoma sp. had a high ability to produce lipase. Ten of tested isolates representing 24.39% of tested fungi had a moderate ability to produce cellulase enzyme. These isolates were belonging to Aspergillus flavus, A. niger, A. parasiticus, A. versicolor, Curvularia 
 lunata, Penicillium griseofulvum, P. oxalicum, P. stekii, P. variabile and Ulocladium atrum. Twenty seven isolates representing 37.64 % of 
 tested isolates belonging to A. flavus, A. fumigatus, A. niger, A. parasiticus, A. terreus, A. versicolor, Fusarium oxysporium, Mucor 
 fuscus, P. glaprum, P. griseofulvum, P. oxalicum, P. variabile, Phoma sp., and U. atrum were moderate invertase producers. Sixteen fungal 
 isolates representing 28.07 % of tested isolates were moderate protease producers, these isolates were belonging to A. flavus, A. 
 niger, A. terreus, A. tamarii, A. versicolor, C. lunata, F. oxysporium, H. insolens, P. corylophilum, P. griseofulvum, P. oxalicum, P. stekii, 
 P. vinaceum, Rhizopus stolonifer. 

Characterization of fungi isolated from cattle dung with potential lignocellulolytic activities

Agricultural wastes not only serve as sources of environmental pollutions, but also serve as magnificent source of plant nutrients and natural repositories of bio-tools especially fungi of industrial interest for lignocellulolytic enzymes production. However, residues management particularly in rice–wheat system is a tedious phenomenon due to narrow gap between harvesting and sowing of these two crops. Hence, microbial intervention is one of the key options that may use successfully for their management. Thus, cellulolytic fungi were isolated from the cattle dung collected from various locations for cellulose manufacturing. The cellulolytic property of fungal isolates was confirmed by plate screening assay based on yellow zone formation surrounding of fungal culture on Congo red agar medium plate owing to production of cellulase enzyme. The colony diameter, clear zone diameter and cellulolytic index showing in descending order of CRD3, CRD5, CRD6 and CRD10 isolates, but –glucosidase activity was greater in CRD3 and CRD5 while, the lowest was in CRD9. However, isolates CRD7, CRD8 and CRD10 exhibited greater biochemical and enzymatic activity than rest of the promising fungal isolates. Similarly, isolates CRD1 and CRD2 utilized most of carbon sources as carbon substrate, but CRD7, CRD8 and CRD10 could not utilized mannitol and cellulose as carbon substrate. Overall, isolate CRD10 showed as a promising isolate in respect of biochemical, carbon utilization pattern and enzymatic activity than rest of the promising isolates.

Exploring and Optimizing the Termite Hill Bacterial Diversity for Cellulase Production

Cellulose is the integral component of lignocellulosic biomass which is found abundantly in nature. The lignocellulosic bioconversion is an emerging technology which utilizes microbes and their enzymes for serving the scientific community. In this study, about 126 isolates were isolated from the termite mound soil among which 81 isolates produced cellulase enzyme. Three isolates B12, B25 and B64 exhibited maximum enzyme activity and chosen for optimization parameters such as temperature, pH and incubation time. The isolates B12, B25 and B64 were identified based on 16S rRNA sequencing as Mesobacillus jeotgali, Lysin, Bacillus fusiformis and Bacillus sp respectively. The temperature, pH and incubation time for maximum cellulase production was optimized from the range and depicted that maximum activity at 40 o C, 7 and 60 hours respectively.

Cellulolytic activity at the low temperature of associative bacteria from a seagrass ecosystem

Cellulase enzymes have been widely used in many industrial activities, such as the production of biofuels, biofertilizers, and bio-decomposers. The usage of cellulase enzymes on an industrial scale usually involves a relatively high temperature. Microorganisms in marine ecosystems are widely known to have the ability to produce cellulase enzymes in various environmental ranges. However, exploring marine cellulolytic bacteria at low temperatures still lacks information. This study aims to determine the cellulolytic activity of bacteria associated with seagrass ecosystems at low temperatures. Cellulolytic activity test used selected bacteria isolates from the seagrass ecosystem (actinobacteria: AA10b, AA11a, AA30a, AB22b; heterotrophic bacterial: HB1.1, HB2.1, HT1.2, HT2.2). The cellulolytic index values were obtained from the clear zone test on CMC 1% medium with congo red staining (actinobacteria) and iodine (heterotrophic bacteria) in different temperature treatments (4°C, 17°C, 20°C, and 40°C). The results showed that all isolates had various cellulolytic index values at different temperatures. There was no bacterial cellulolytic activity at 4°C. However some isolates showed activities at 17°C and increased as the temperature was higher. The highest production of cellulolytic activity occurred at 40°C for both actinobacteria and heterotrophic bacteria isolates. The actinobacteria isolate AA11a had the highest cellulolytic index of 7.00 at 40°C. This study indicates that at low temperatures, associative bacteria from the seagrass ecosystem have considerable cellulolytic activity for further research applications in various industries.

Prospects of microbial cellulase production using banana peels wastes for antimicrobial applications

Microorganisms have been extensively studied and used to produce a wide range of enzymes and bioactive substances for a number of uses. Cellulases have also been widely used for a variety of bioprocessing and biotransformation purposes and are acknowledged as the essential enzymes for industrial applications. Broad industrial applications and huge demand essentially require mass-scale and low-cost production of cellulase enzyme. Nevertheless, low-cost production of cellulase enzyme at industrial-level finds certain issues, and this may be mainly associated with the unavailability of cheap and effective substrate to be utilized in fermentation process. In this context, cellulosic wastes are counted as one of the suitable bioresources and have been well explored for low-cost and highly efficient cellulase enzyme productions. Further, banana peels waste is considered as the high cellulose & sugar containing food wastes which is renewable and hugely available worldwide. Therefore, the present review explores the possible utilizations of banana peels as a potential food waste to be employed as substrate to produce cellulase enzymes. Availability and compositional analysis of banana peels has been explored for the microbial cellulase production based on reported studies. Further, this review explores the applications of cellulase enzymes as antimicrobial agents. Based on the available studies and their evaluation, potential limitations and future suggestions for the production of cellulase enzymes and their applications as antibacterial agents have been provided, which have a high potential for numerous biomedical applications and may offer a new opportunity for industrial utility.

Optimization of Cellulase Production by Aspergillus niger InaCC F506 in Solid-State Fermentation of Tofu Dreg

Indonesia has a growing demand for cellulase enzymes; however, 99% of the enzymes are imported from other countries. Aspergillus niger is well recognized for using the widely accessible tofu by-product, often known as tofu dreg, as a growth medium for synthesizing cellulase enzymes. This study aims to optimize the production of cellulase enzymes by Aspergillus niger InaCC F506 using tofu dregs as a substrate through the Solid-State Fermentation (SSF) method by varying the additives. The results showed that the E fermentation system with the composition of urea 0.5%; CMC 0.5%; KH2PO4 0.2%; MgSO4.7H2O 0.2% produced the highest cellulase enzymes from the tofu dregs substrate. The highest cellulase enzyme activity was at a fraction of ammonium sulfate saturation level of 40-60%. The optimum condition of enzyme activity was observed at pH 5 with an activity of 33 x 10-4 Units/mg protein and at 30℃ with an activity of 31 x 10-4 Units/mg protein.

On-site enriched production of cellulase enzyme using rice straw waste and its hydrolytic performance evaluation through systematic dynamic modeling.

The application of on-site produced cellulolytic enzymes in place of commercial enzymes towards hydrolytic preparations of reducing sugars using inexpensive lignocellulosic wastes is considered the most efficient strategy to accomplish a cost-effective biofuel production process. Along with improved production, intrinsic and systematic performance evaluation of the produced enzyme during the hydrolysis process through kinetic intervention remains a crucial requirement for achieving the improved performance of the process. With this motivation, the present study primarily deals with the nutritionally optimized production strategy of cellulases from rice straw (RS) waste using Trichoderma reesei (MTCC 164). The highest cellulase production was obtained 8.09 ± 0.32g/l in batch mode at optimized combinations of 3.5% (w/v) RS inducer, 3.0% (w/v) lactose, and 1.5% (w/v) peptone. Production was further improved through pH-regulated (pH 5.5 to 6.5) fed-batch fermentations. The enzyme produced at pH 6 was considered for hydrolysis studies at 4 to 10% (w/w) solid loading due to reasonable exoglucanase, endoglucanase, and maximum β-glucosidase activity levels of 9.3 U/ml, 3.87 U/ml, and 2.65 U/ml respectively. Multi-reaction systematic kinetic modeling was implemented to evaluate enzyme performance during hydrolysis, and the values of inhibitory kinetic parameters (K2r = 7.1 < K1r = 18.5 < K3r = 276.6) suggested that sequential conversion of cellulose to glucose by existing enzyme components was more dominant over direct conversion.

Optimization of Production Conditions of Cellulase Enzyme from Micro-Fungi Aspergillus Fumigatus for Agriculture Application

Cellulase enzymes are belonging to the hydrolytic group of enzymes facilitates the sugar release and its bioconversion into different valuable industrial products. Isolated micro fungi from rice straw by dilution plating pouring method studied for playing a various role in industries as well as in agriculture application. Various micro-fungi show enzymatic degradation of lignocellulosic material. The present study optimized the growing conditions for cellulase enzymes production from A.fumigatus. Optimization of various growth conditions such as temperature, different pH level and nitrogen source were studied for the production of enzyme carboxymethyl cellulase during this study. The result showed that A. fumigatus produced highest cellulase activity (3.546 IU/ml) at pH 7.0 and temperature 300C with yeast extract and Fpase activity (0.653 IU/ml) through solid state fermentation. In future agriculture applications and in industries the cellulase enzyme production attains a crucial role to acquire biodegradable yield.

A Combined Study on Optimization, In Silico Modeling, and Genetic Modification of Large Scale Microbial Cellulase Production.

Cellulase is a biocatalyst that hydrolyzes cellulosic biomass and is considered a major group of industrial enzymes for its applications. Extensive work has been done on microbial cellulase but fungi are considered a novel strain for their maximum cellulase production. Production cost and novel microbial strains are major challenges for its improvement where cheap agro wastes can be essential sources of cellulose as substrates. The researcher searches for more cellulolytic microbes from natural sources but the production level of isolated strains is comparatively low. So genetic modification or mutation can be employed for large-scale cellulase production before optimization. After genetic modification than in silico molecular modeling can be evaluated for substrate molecule's binding affinity. In this review, we focus not only on the conventional methods of cellulase production but also on modern biotechnological approaches applied to cellulase production by a sequential study on common cellulase-producing microbes, modified microbes, culture media, carbon sources, substrate pretreatment process, and the importance of optimum pH and temperature on fermentation. In this review, we also compare different cellulase activity determination methods. As a result, this review provides insights into the interrelationship between the characteristics of optimizing different culture conditions, genetic modification, and in silico enzyme modeling for the production of cellulase enzymes, which may aid in the advancement of large-scale integrated enzyme manufacturing of substrate-specific enzymes.

OPTIMIZATION OF ASPERGILLUS TUBINGENSIS GROWTH FOR CELLULASE PRODUCTION

Celluloses are the basic component of plant cell walls which are difficult to break however, cellulase enzymes have the ability to degrade the cellulose into glucose. The use of celullase enzymes is spreading widely particularly in the paper, pulp and food industries to reduce biowaste. The purpose of this study was to optimize the production of cellulase enzymes by using fungal strain Aspergillus tubingensis. The fungal strain Aspergillus tubingensis was extracted from putrid fruits and vegetable samples collected from the local fruit market of Pattoki in the District Kasur. The identification of fungus was carried out using microscope. Corn Stover was used as a substrate for cellulase production. Different parameters were used for the optimization of fungus growth such as substrate level, substrate particle size, temperature, pH, moisture, urea and glucose. Cellulase enzymes were purified by salting out and gel filtration techniques. The optimum substrate level, substrate particle size, temperature, pH, glucose, urea and moisture were analyzed as 5g, 40mm, 40°C, 4.5pH, 0.5g, 0.04g and 60% respectively. The maximum activity reveled by cellulase enzyme at different optimized parameters were reported in U/mL/min. The cellulase molecular weight was determined 71 kDa by Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis. It was concluded that the study will help to a way for the scientists for the maximum production of cellulase from Aspergillus tubingensis using Corn stover as substrate and most cost-effective Solid State Fermentation method.

Solid-state fermentation of Saba banana peel for pigment production by Monascus purpureus.

Eco-friendly natural pigment demand has ever-increasing popularity due to health and environmental concerns. In this context, the aim of this study was to evaluate the feasibility use of Saba banana peel as low-cost fermentable substrate for the production of pigments, xylanase and cellulase enzymes by Monascus purpureus. Among the strains tested, M. purpureus TISTR 3385 produced pigments better and had higher enzyme activities. Under the optimal pigment-producing conditions at the initial moisture content of 40% and initial pH of 6.0, the pigments comprising yellow, orange, and red produced by the fungi were achieved in the range of 0.40-0.93 UA/g/day. The maximum xylanase and cellulase activities of 8.92 ± 0.46 U/g and 4.72 ± 0.04 U/g were also obtained, respectively. More importantly, solid-state fermentation of non-sterile peel could be achieved without sacrificing the production of the pigments and both enzymes. These indicated the potential use of the peel as fermentable feedstock for pigment production by the fungi and an environmental-friendly approach for sustainable waste management and industrial pigment and enzyme application.

Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes Pleurotus ostreatus and Phanerochaete chrysosporium under Submerged Fermentation

Cellulase enzymes attract a lot of research due to their industrial application. Diverse cellulase-producing organisms and substances that induce cellulase are highly sought after. This study aimed to evaluate the effect of different inducer sources on cellulase production by white rot fungi P. ostreatus CGMCC 3.7292 and P. chrysosporium CGMCC 3.7212 under submerged fermentation employing a completely randomized experimental design. The different inducer sources tested were nitrogen (yeast, potassium nitrate, sodium nitrate, ammonium sulphate, aqueous ammonia and urea), carbon (malt extract, glucose, fructose, carboxymethylcellulose, starch and xylose) and agro-biomass (stevia straw, wheat straw, oat straw, alfalfa straw, corn cobs and corn stover). These inducer sources strongly impacted enzyme activities by P. ostreatus CGMCC 3.7292 and P. chrysosporium CGMCC 3.7212. The suitable nitrogen and carbon inducer sources for cellulase activity by P. ostreatus and P. chrysosporium were yeast (1.354 U/mL and 1.154 U/mL) and carboxymethylcellulose (0.976 U/mL and 0.776 U/mL) while the suitable agro-biomass were wheat straw (6.880 U/mL) and corn stover (6.525 U/mL), respectively. The least inducer sources in terms of nitrogen, carbon and agro-biomass for cellulase activity by P. ostreatus and P. chrysosporium were urea (0.213 U/mL and 0.081 U/mL), glucose (0.042 U/mL and 0.035), xylose (0.042 U/mL and 0.035 U/mL) and stevia straw (1.555 U/mL and 0.960 U/mL). In submerged fermentation, the cellulase enzyme activity of P. ostreatus in response to various inducer sources was relatively higher than P. chrysosporium.

Cocultivation of White-Rot Fungi and Microalgae in the Presence of Nanocellulose.

ABSTRACTCocultivation of fungi and algae can result in a mutualistic or antagonistic interaction depending on the species involved and the cultivation conditions. In this study, we investigated the growth behavior and enzymatic activity of two filamentous white-rot fungi (Trametes versicolor and Trametes pubescens) and two freshwater algae (Chlorella vulgaris and Scenedesmus vacuolatus) cocultured in the presence of TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) oxidized cellulose nanofibrils (CNF) and cellulose nanocrystals (CNC). The growth of fungi and algae was studied in liquid, agar medium, and 3D-printed nanocellulose hydrogels. The results showed that cocultures grew faster under nutrient-rich conditions than in nutrient-depleted conditions. Key cellulose-degrading enzymes, including endoglucanase and laccase activities, were higher in liquid cocultures of T. versicolor and S. vacuolatus in the presence of cellulose compared to single cultures of fungi or algae. Although similar results were observed for cocultures of T. pubescens and C. vulgaris, laccase production diminished over time in these cultures. Fungi and algae were capable of growth in 3D-printed cellulose hydrogels. These results showed that cellulase enzyme production could be enhanced by cocultivating white-rot fungi with freshwater algae under nutrient-rich conditions with TEMPO-CNF and CNC. Additionally, the growth of white-rot fungi and freshwater algae in printed cellulose hydrogels demonstrates the potential use of fungi and algae in hydrogel systems for biotechnological applications, including biofuel production and bio-based fuel cell components.IMPORTANCE Depending on the conditions used to grow fungi and algae in the lab, they can interact in a mutually beneficial or negative way. These interactions could stimulate the organisms to produce enzymes in response to the interaction. We studied how wood decay fungi and freshwater algae grew in the presence and absence of cellulose, one of the basic building blocks of wood. How fungi and algae grew in 3D-printed cellulose hydrogels was also tested. Our results showed that fungi and algae partners produced significantly larger amounts of enzymes that degraded cellulose when grown with cellulose than when grown alone. In addition, fungi and algae were shown to grow in dense nanocellulose hydrogels and could survive the shear conditions during gel structuring while 3D-printing. These cultures could potentially be applied in the biotech industry for applications like energy production from cellulose, biofuel production, and bioremediation of cellulose material.

UTILIZATION OF AGRICULTURAL WASTE AS A SUBSTRATE FOR PRODUCING CELLULASE ENZYME BY ASPERGILLUS NIGER

Fresh Fruit Bunches (FFB) of oil palm, bran, straw, and bagasse are agricultural wastes whose availability is very abundant in Indonesia. The agricultural waste is lignocellulosic waste which still has economic value if further processing is carried out, namely as a substrate in the production of cellulase enzymes. Cellulase enzymes are commonly used in various industries such as food biotechnology, textiles, animal feed, paper, and agriculture to degrade cellulose with its main products, namely glucose, cellobiose, and cellooligosaccharides. In producing cellulase enzymes, it is necessary to have microorganisms that have a high ability to produce enzymes, one of which is Aspergillus niger. The purpose of this study was to determine the activity of crude cellulase enzyme, protein content, and specific activity of cellulase enzyme from agricultural waste which includes FFB, bran, straw, and bagasse. The research methods included preparation of Aspergillus niger culture, delignification, basal medium preparation, cellulase enzyme production, enzyme extraction, crude cellulase enzyme activity test (CMC-ase), lowry method protein content test, and determination of cellulase enzyme specific activity. The study showed that the highest crude cellulase enzyme activity in bran was 26.83 U/ml, the highest protein content in bagasse was 63.42 g/ml, and the highest specific activity of cellulase enzyme in straw was 0.9818 U/ml. The high enzyme activity is influenced by the cellulose content in the material, type of substrate, media, substrate concentration, pH, and temperature.

Participation of type VI secretion system in plant colonization of phosphate solubilizing bacteria

In mutualistic endophytic bacteria, the type VI secretion system (T6SS) is related to important functions, such as interbacterial competition, stress response, quorum sensing, biofilm formation, and symbiosis. The presence of T6SS in beneficial endophytic bacterial population associated with different plants suggests that it plays an important role in its interaction with the eucaryotic partner. Within plant promoting bacteria, those with phosphate solubilizing activity constitute a group of great relevance to the rhizosphere as they provide phosphorus to plants. Among them, those with endophytic colonization capacity have survival advantages. The aim of this study was to determine whether the T6SS of a native peanut phosphate solubilizing bacterium is involved in its colonization in this legume. Initially, an in silico analysis looking for genes related to T6SS in the genome of the Enterobacter sp. J49 strain enabled us to identify almost all the tss genes, except for the tssE gene. A T6SS mutant of the Enterobacter sp. J49 strain was obtained by interrupting one of the essential tss genes. Then, the Enterobacter sp. J49-hcp strain was inoculated on peanut plants to analyze its colonization capacity. In addition, properties associated with endophytic colonization were analyzed, such as the formation of biofilms and the production of pectinase and cellulase enzymes. The results obtained indicated a significant decrease in the epiphytic and endophytic colonization of the mutant with respect to the wild strain. It is possible to conclude that T6SS, although not essential, may participate in bacterial colonization, either by accelerating the infection or by promoting other mechanisms involved in it.

Production and optimisation of cellulase enzyme by Aspergillus uvarum CBS 121591 isolated from soil

Production and optimisation of cellulase enzyme by Aspergillus uvarum CBS 121591 isolated from soil

CELLULOSOLYTIC ACTIVITY OF SOILS OF THE ODESA CITY

Problem Statement and Purpose. Cellulose cleavage is of great importance in the carbon cycle, as it contains more than 50% of all organic carbon in the biosphere. This is a fairly stable organic compound that is destroyed only by very strong oxidants. Under natural conditions, the destruction of cellulose is carried out by various microorganisms that produce cellulase enzymes. The activation of cellulose decomposition is influenced by temperature, pH level, soil aeration, biological properties of vegetation, etc. Being very resistant to physicochemical factors, cellulose is easily decomposed by microorganisms with the release of carbon, which in the form of various compounds is involved in creating soil fertility. Cellulose is broken down by aerobic microorganisms (bacteria and fungi) and anaerobic mesophilic and thermophilic bacteria. The peculiarity of cellulose-destroying microorganisms is their high demand for nitrogen sources. Soil-destroying soil microorganisms are the most important suppliers of organic matter to various groups of microorganisms (including nitrogen-fixing) in the common food chain. Since the activity of cellulose-destroying microorganisms also depends on the presence of available phosphorus and other elements in the soil, the degree of decomposition of fiber can be considered to reflect the direction of microbiological processes in general. Cellulolytic activity indicates the intensity of biological processes in the soil. The more intensively the cellulose decomposes, the faster the biological cycle of elements takes place and the fuller the plants are provided with nutrients. Therefore, cellulose activity is used as one of the indicators of biological activity of the soil environment. Data &amp;Methods. The author, in the study of cellulosolytic activity of soils in the city of Odessa, used the method of applications (laying in the soil strips of filter paper or linen cloth fixed on the glass). Applications were laid vertically in the upper 15-cm layer of soil of 5 pieces on the site. A month later, they were dug up, carefully washed from the soil and decay products, dried and weighed again. Cellulolytic activity was determined by weight loss of exposed tissue. Results. As a result of research on the study of cellulolytic activity of soils in the city of Odessa, it was found that cellulolytic activity is an important indicator of the intensity of destructive processes in the soil. The intensity of cellulose decomposition in the soil is determined by the combined action of several factors: weather conditions, the nature of vegetation, the amount of organic matter entering the soil, soil type, its physical properties, chemical composition. In an urban environment, the intensity of cellulosolytic processes is also regulated by the nature and degree of anthropogenic impact on soil, atmosphere and vegetation. The intensity of cellulose destruction in the city soils is estimated to be very weak, weak and medium. The cellulolytic processes in the soils of the city of Odessa in 2021 were stimulated by abundant precipitation (r = 1,000). The decrease in CA in some parts of the city may be due to minimal anthropogenic impact on the soil cover.

Probiotic characteristics of Bifidobacterium infantis isolated in Vietnam

Bifidobacterium is anerobic bacterium which is popular in human and animal intestine. B.&amp;nbsp;infantis was successfully isolated from a new-born child&amp;rsquo;s fecal sample previously. In this study, the probiotic characteristics of this strain such as: extracellular enzyme production ability; antibiotic activity against food-born pathogen like E. coli, S. aureus; organic acid production and bile-salt sufferance were evaluated. The results showed that the isolated can produce amylase and protease enzyme, do not produce cellulase enzyme; the organic acids also were produced during development of bacterium. The bacterium can against E. coli and S. aureus and can grow in the media containing up to 1% of bile salt. Results of this study are the basis for further studies to apply these bacteria as a source of probiotic beneficial to human health in Vietnam.

Isolasi Bakteri Selulolitik Asal Saluran Pencernaan Rayap Dan Daya Hidupnya Pada Berbagai Substrak Yang Berasal Dari Limbah Pertanian Dan Perkebunan

ABSTRACT This study aimed to identify isolates that produce cellulase enzymes derived from cellulolytic bacteria from the digestive tract of termites and to determine the best type of substrate as the carrier and survival of these isolates. This research consists of 3 (three) stages. The first stage of research was the isolation of cellulolytic bacteria using descriptive data analysis. The second stage of research was to determine the best carrier for selected cellulolytic isolates, while the third stage was to determine the shelf life of the isolates with the selected substrate as the best carrier by analyzing data using analysis of variance. The results of the first phase of research obtained 3 isolates of cellulolytic bacteria from the best termite digestive tract based on the clear zone index test with isolate code VR52, VR61 and VR81 with clear zone index for each isolate 1.087; 2,250 and 1,710 mm. Based on Gram staining, the three isolates were gram-positive bacteria in the form of bacilli. Maximum enzyme activity in isolate VR61 (Bacillus sp) with its enzyme activity (19.91 µ/mL). The results of the second stage study showed that rice bran was the best sAubstrate based on total microbes with a value of 202 x 109 CFU / g. The results of the third stage research showed that the number of cellulolytic bacteria in the selected sub-extract (rice bran) at a storage temperature of 4oC (178.92 x 109 CFU / gr) was more than that of storage at 27oC (101.69 x 109 CFU / gr). This research concludes that the longer the storage time shows a decrease in the number of bacteria and pH in the sub-extract. The best interaction on rice bran sub-extract as a carrier at a storage temperature of 4oC in the second week with the number of cellulolytic bacteria 197.8 x 109 CFU / gr with a pH value of 6.46 and the fourth week of 167.85 x 109 CFU / gr with a pH value of 6.39. Keywords: Cellulose, Cellulolytic Bacteria, Termites