Production Methods Research Articles

Eco-Friendly Poly (Butylene Adipate-co-Terephthalate) Coated Bi-Layered Films: An Approach to Enhance Mechanical and Barrier Properties.

In this research work, a coated paper was prepared with poly (butylene adipate-co-terephthalate) (PBAT) film to explore its use in eco-friendly food packaging. The paper was coated with PBAT film for packaging using hot pressing, a production method currently employed in the packaging industry. The coated papers were evaluated for their structural, mechanical, thermal, and barrier properties. The structural morphology and chemical analysis of the coated paper confirmed the consistent formation of PBAT bi-layered on paper surfaces. Surface coating with PBAT film increased the water resistance of the paper samples, as demonstrated by tests of barrier characteristics, including the water vapor transmission rate (WVTR), oxygen transmission rate (OTR), and water contact angle (WCA) of water drops. The transmission rate of the clean paper was 2010.40 cc m-2 per 24 h for OTR and 110.24 g m-2 per 24 h for WVTR. If the PBAT-film was coated, the value decreased to 91.79 g m-2 per 24 h and 992.86 cc m-2 per 24 h. The hydrophobic nature of PBAT, confirmed by WCA measurements, contributed to the enhanced water resistance of PBAT-coated paper. This result presents an improved PBAT-coated paper material, eliminating the need for adhesives and allowing for the fabrication of bi-layered packaging.

A VHH single-domain platform enabling discovery and development of monospecific antibodies and modular neutralizing bispecifics against SARS-CoV-2 variants

Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to evolve, escape coronavirus disease 2019 therapeutics and vaccines, and jeopardize public health. Methods To combat SARS-CoV-2 antigenic escape, we developed a rapid, high-throughput pipeline to discover monospecific VHH antibodies and iteratively develop VHH-Fc-VHH bispecifics capable of neutralizing emerging SARS-CoV-2 variants. Results By panning VHH single-domain phage libraries against ancestral or Beta spike proteins, we discovered high-affinity VHH antibodies with unique target epitopes. Combining two VHHs into a tetravalent bispecific construct conferred broad neutralization activity against multiple variants and was more resistant to antigenic escape than the monospecific antibody alone. Following the rise of the Omicron variant, a VHH in the original bispecific construct was replaced with another VHH discovered against Omicron BA.1 receptor binding domain—the resulting bispecific exhibited neutralization against both BA.1 and BA.5 sublineage variants. Conclusions A heavy chain-only tetravalent VHH-Fc-VHH bispecific platform derived from humanized synthetic libraries held a myriad of unique advantages: 1) synthetic preconstructed libraries minimized risk of liabilities and maximized discovery speed, 2) VHH scaffolds allowed for a modular “plug-and-play” format that could be rapidly iterated upon as variants of concern arose, 3) natural dimerization of single VHH-Fc-VHH polypeptides allowed for straightforward bispecific production and purification methods, and 4) multivalent approaches enhanced avidity boosting effects and neutralization potency, and conferred more robust resistance to antigenic escape than monovalent approaches against specific variants. This iterative platform of rapid VHH discovery combined with modular bispecific design holds promise for long-term viral control efforts.

Comparison of different hydrogen-ammonia energy conversion pathways for renewable energy supply

Hydrogen and ammonia are considered promising energy carriers for renewable energy due to their environmental friendliness and medium- and long-term storage cost advantages. However, a single hydrogen or ammonia energy strategy generally cannot meet the demand, limiting widespread application. Here we consider the whole process of renewable energy generation-transmission-storage-consumption, and propose a hydrogen-ammonia combined operation strategy. Assuming a rated power of 40 MW for generation and 10 MW for demand, we compare five energy conversion pathways: hydrogen or ammonia pipeline transmission, high-voltage alternating current (HVAC) transmission with hydrogen or ammonia storage, and HVAC transmission with hydrogen-ammonia hybrid energy storage. Results indicate that for a transmission distance of 100 km, the most economical pathway is HVAC transmission with hydrogen-ammonia hybrid energy storage, with a net present value (NPV) of 39.31 M$ and a levelized cost of electricity (LCOE) of 0.081 $/kWh. Sensitivity analyses reveal that a 0.01 $/kWh increase in electricity price leads to NPV growths of 11.75–16.02 M$. This pathway achieves zero carbon footprint and saves 4.7 M$ annually in carbon taxes. Compared to traditional production methods, this pathway is equivalent to reducing emissions by 94,189 tons.

Chitosan-Chitosan derivative for cartilage associated disorders: Protein interaction and biodegradability

Chitosan is a well-known biomaterial in the field of cartilage tissue engineering because of its flexibility. The construction of chitosan-based scaffolds is reviewed in several production methods, including freeze-drying, gelation, salt leaching, and electrospinning. In this review, many benefits of chitosan are discussed, including the interaction of chitosan with other materials and their effects, its adaptability, high reproducibility, biocompatibility, role in cellular differentiation, interactions with TGF-β (transforming growth factor-β), protein interactions, biodegradability, influencing osteoblast expression, and potential for treating bone diseases. The study also provides information on how mathematical models are used to analyze how MSC (mesenchymal stem cells) and chondrocyte distribution in multilayer hydrogels change in response to TGF-β diffusion. Results showed that collagen has weak mechanical properties and easily disintegrates during bone tissue formation, it is difficult to use alone as a biomaterial, and new scaffolds made of whey protein isolate (WPI) and chitosan may help repair osteochondral tissue. However, the thorough analysis reveals chitosan's crucial contribution to the development of cartilage tissue engineering as well as its potential to address issues in the field.

High production of ectoine from methane in genetically engineered Methylomicrobium alcaliphilum 20Z by preventing ectoine degradation

BackgroundMethane is a greenhouse gas with a significant potential to contribute to global warming. The biological conversion of methane to ectoine using methanotrophs represents an environmentally and economically beneficial technology, combining the reduction of methane that would otherwise be combusted and released into the atmosphere with the production of value-added products.ResultsIn this study, high ectoine production was achieved using genetically engineered Methylomicrobium alcaliphilum 20Z, a methanotrophic ectoine-producing bacterium, by knocking out doeA, which encodes a putative ectoine hydrolase, resulting in complete inhibition of ectoine degradation. Ectoine was confirmed to be degraded by doeA to N-α-acetyl-L-2,4-diaminobutyrate under nitrogen depletion conditions. Optimal copper and nitrogen concentrations enhanced biomass and ectoine production, respectively. Under optimal fed-batch fermentation conditions, ectoine production proportionate with biomass production was achieved, resulting in 1.0 g/L of ectoine with 16 g/L of biomass. Upon applying a hyperosmotic shock after high–cell–density culture, 1.5 g/L of ectoine was obtained without further cell growth from methane.ConclusionsThis study suggests the optimization of a method for the high production of ectoine from methane by preventing ectoine degradation. To our knowledge, the final titer of ectoine obtained by M. alcaliphilum 20ZDP3 was the highest in the ectoine production from methane to date. This is the first study to propose ectoine production from methane applying high cell density culture by preventing ectoine degradation.

Green synthesis of functionalized graphene-based material with dimethyl but-2-ynedioate for electrochemical energy storage devices

Functionalization of graphene with dimethyl acetylenedicarboxylate is achieved through a microwave-assisted Diels-Alder reaction. The physical, chemical, and electrochemical properties of the modified sheets are thoroughly investigated by complementary characterization techniques. Density Functional Theory calculations are employed to examine the functionalization mechanism and to highlight the role of defects such as epoxide bridges introduced in graphene during exfoliation. Our findings provide valuable insights into the development of efficient and cost-effective methods for large-scale production of high-quality graphene-based materials. Specifically, the electrochemical properties of anode materials containing functionalized graphene are evaluated for Li-ion electrochemical energy storage devices, demonstrating excellent electrochemical reversibility and rate capability. The cyclic voltammetry analysis reveals material stabilization after a few cycles, resulting in a coulombic efficiency of up to 95 % and a discharge capacity of 162.3 mA·h·g−1. The galvanostatic cycling test indicates that the material electrode retains 57 % of its initial capacity at a C-rate of 10C, indicating high-power capability. These promising results position organic modified graphene as a potential material for Li-ion capacitors, with a specific capacity that aligns with the last intercalation stage capacity at a lower potential. Overall, the study's findings offer significant contributions to the advancement of graphene-based materials in energy storage applications.

Influence of Laser Texturing and Coating on the Tribological Properties of the Tool Steels Properties

The article is aimed at identifying the influence of laser texturing and subsequent coating with a hard, wear-resistant coating AlCrSiN (nACRo®) on selected tribological properties of the analyzed tool steels for cold work, produced by conventional and powder metallurgy. The substrate from each steel was heat treated to achieve optimal properties regarding the chemical composition and the method of production of the material. Böhler K100 and K390 Microclean® steels were used. These are highly alloyed tool steels used for various types of tools intended for cold work. The obtained results show that the coefficient of friction is increased by coating, but the wear rate is lower compared to the samples which were only textured.

Liquid Metal Electrode Ink for Printable Lithium‐Ion Batteries

Recent advancements have significantly improved the flexibility of lithium‐ion batteries (LIBs). The battery electrode contains a reaction layer and a current collector layer. Flexible battery electrodes, in which these layers are integrated, are fabricated in a single process, simplifying the electrode fabrication process. However, to date, integrated electrodes have lower conductivity than conventional two‐layer electrodes, and the process that maintains conductivity has not been simplified. This study proposes a liquid metal electrode ink that simultaneously plays the role of the reaction and current collector layers with high conductivity and can be formed in a single process via printing. It employs a gallium‐based liquid metal as a liquid current collector, embedded with Li4Ti5O12 or Li2TiS3 as active materials. These liquid metal electrodes exhibit a high electronic conductivity of 104 S cm−1 and can be fabricated without requiring the lengthy postprocessing time associated with conventional inks. Moreover, the ink can be applied directly to various insulating and nonflat curved substrates, demonstrating its charge–discharge performance, even when bent to a 1 mm radius to maintain flexibility. Liquid metal‐based electrode inks simplify the LIB fabrication process, contributing to efficient production methods for electrodes in future LIBs.

Technological lock-in developing countries: The role of external financing

Liabilities denominated in foreign currency have established a permanent role in emerging market firms’ balance sheet, which implies that changes in both global liquidity conditions and in the value of the currency may have a long- lasting effect for them. In order to consider the financial conditions that may encourage (discourage) structural change in a small, open economy, we adopt the framework put forward by the “Monetary theory of distribution” (MTD). More specifically, we follow the formulation adopted by Dvoskin and Feldman (2019), whereby the financial system is intended as a basic sector that promotes innovation (Schumpeter, 1911). In accordance to this, financial conditions are binding only for the innovative entrepreneurs, whose methods of production are not dominant and hence they need to borrow from banks to kick- start their production. Through this device, our model offers an explanation to the technological lock-in experienced by a small, open economy which takes international prices as given.

Unraveling a Historical Mystery: Identification of a Lichen Dye Source in a Fifteenth Century Medieval Tapestry

As part of a long-term campaign to document, study, and conserve the Heroes tapestries from The Cloisters collection at The Metropolitan Museum of Art, organic colorant analysis of Julius Caesar (accession number 47.101.3) was performed. Analysis with liquid chromatography–quadrupole time-of-flight mass spectrometry (LC-qToF-MS) revealed the presence of several multiply chlorinated xanthones produced only by certain species of lichen. Various lichen dye sources have been documented in the literature for centuries and are classified as either ammonia fermentation method (AFM) or boiling water method (BWM) dyes based on their method of production. However, none of these known sources produce the distinctive metabolites present in the tapestry. LC-qToF-MS was also used to compare the chemical composition of the dyes in the tapestry with that of several species of crustose lichen. Lichen metabolites, including thiophanic acid and arthothelin, were definitively identified in the tapestry based on comparison with lichen xanthone standards and a reference of Lecanora sulphurata, confirming the presence of a lichen source. This finding marks the first time that lichen xanthones have been identified in a historic object and the first evidence that BWM lichen dyes may have been used prior to the eighteenth century.

Challenges of the integrative product and production system development

AbstractShorter product lifecycles and a shift from mechatronic to cyber-physical systems are leading to greater product complexity. This complexity can be addressed by more intensive cooperation between product and production system development. Despite intensive development in recent years, these process models have not been able to establish themselves in corporate practice. Therefore, this paper analyses the existing integrative product and production methods to identify the reasons for their lack of use in practice. The analysis has shown that there are nine barriers.

Assessment of the Environmental Footprint of Various Food Production Systems

Purpose: This study sought to assess the environmental footprint of various food production systems. Methodology: The study adopted a desktop research methodology. Desk research refers to secondary data or that which can be collected without fieldwork. Desk research is basically involved in collecting data from existing resources hence it is often considered a low cost technique as compared to field research, as the main cost is involved in executive’s time, telephone charges and directories. Thus, the study relied on already published studies, reports and statistics. This secondary data was easily accessed through the online journals and library. Findings: The findings reveal that there exists a contextual and methodological gap relating to the environmental footprint of various food production systems. Preliminary empirical review revealed that with a call for transformative changes to mitigate ecological impacts while ensuring food security and human well-being. Through a comprehensive analysis, significant variations in resource use and emissions were highlighted across different production methods. The study emphasized the interconnectedness between dietary choices, agricultural practices, and environmental outcomes, urging policymakers to enact evidence-based policies and agricultural stakeholders to adopt regenerative farming practices. Ultimately, the study served as a call to action for concerted efforts to transition towards a more sustainable and resilient food system, integrating ecological principles, social equity, and economic viability to promote harmony between human activities and the natural environment. Unique Contribution to Theory, Practice and Policy: The Systems theory, Ecological Economics and Agroecology models may be used to anchor future studies on the environmental footprint of various food production systems. The study made significant contributions to theory, practice, and policy in the realm of sustainable food production and environmental conservation. It advanced theoretical understanding by advocating for a systems-based approach to assessing environmental impacts and highlighting the importance of considering multiple dimensions of sustainability. In practice, the study recommended the adoption of agroecological approaches and the promotion of sustainable farming practices to mitigate environmental harm. Policy recommendations included the implementation of regulatory interventions and incentives to promote sustainability, while consumer education and international collaboration were emphasized for driving demand for sustainable food products and facilitating knowledge sharing. Lastly, the study underscored the importance of investment in research and innovation to drive continuous improvements in sustainable food production systems.

An Exploration of various Fructooligosaccharides production methods using novel engineered enzymes with innovative core-shell chitosan beads

This study investigates Fructooligosaccharides (FOSs) production as prebiotics to promote gut health and overall well-being. Various production methods for FOSs were explored, leveraging novel engineered inulosucrase (IN) and levansucrase (LV) enzymes. The methodologies included batch stirred reactors with free enzymes and fixed-bed reactors utilizing enzymes immobilized on hydrogel chitosan beads (HGBs) and core-shell chitosan beads (CSBs). The study found that free enzymes exhibited the highest sucrose consumption rates, with IN achieving 142.8 g/L∙h and LV achieving 82.9 g/L∙h. However, CSB-immobilized enzymes demonstrated substantial enzyme savings (55% and 41% reduction in IN and LV consumption, respectively) through reuse across multiple cycles, while delivering superior FOSs yields. CSB-immobilized enzymes outperformed HGB-immobilized enzymes in terms of immobilization efficiency, activity recovery, sucrose conversion, FOSs yields, and repeatability. A novel reactor setup involving interconnected fixed-bed reactors with different enzymes in series produced promising results, generating novel FOSs species alongside expected I-FOSs and L-FOSs. Consumable cost analysis indicated that while free enzyme usage is initially cost-effective (5.09–7.06 USD/kg FOSs), significant increases in LV prices could make CSB immobilization a cost-competitive alternative. This study highlights the potential of innovative enzyme immobilization strategies and reactor designs for efficient FOSs production, considering both performance and economic feasibility.

Generation of stable suspension producer cell lines for serum-free lentivirus production.

The production of lentiviral vectors (LVs) pseudotyped with the vesicular stomatitis virus envelope glycoprotein (VSV-G) is limited by the associated cytotoxicity of the envelope and by the production methods used, such as transient transfection of adherent cell lines. In this study, we established stable suspension producer cell lines for scalable and serum-free LV production derived from two stable, inducible packaging cell lines, named GPRG and GPRTG. The established polyclonal producer cell lines produce self-inactivating (SIN) LVs carrying a WAS-T2A-GFP construct at an average infectious titer of up to4.64×107TU mL-1in a semi-perfusion process in a shake flask and can be generated in less than two months. The derived monoclonal cell lines are functionally stable in continuous culture and produce an average infectious titer of up to 9.38×107TU mL-1in a semi-perfusion shake flask process. The producer clones are able to maintain a productivity of>1×107TUmL-1day-1for up to 29 consecutive days in a non-optimized 5L stirred-tank bioreactor perfusion process, representing a major milestone in the field of LV manufacturing. As the producer cell lines are based on an inducible Tet-off expression system, the established process allows LV production in the absence of inducers such as antibiotics. The purified LVs efficiently transduce human CD34+ cells, reducing the LV quantities required for gene and cell therapy applications.

Lithic projectile technology in the western Late Epigravettian: The case study of north-eastern Italy

Projectile implements are one of the most frequent transformed lithic blanks within the Upper Paleolithic assemblages. Since the first taxonomic studies, their morphological variability has been used as primary fossil director to define specific regional trends and diachronic phases. However, an exhaustive analysis of manufacturing methods and techniques applied to shape this gear category is extremely rare. In fact, during the Late Epigravettian, except for a certain typological variability, the lack of detailed studies resulted in an apparent homogeneity in armatures production. In this paper, a technological and traceological approach applied to reconstruct the whole chaîne opératoire of armatures manufacture in three Northern Italian sites dated between 17,000 and 12,000 cal BP demonstrates important variations in how projectile implements are fashioned, concerning shape, production methods and retouch techniques. Moreover, a large-scale comparison suggests how renewals in projectile implements production in North-Eastern Italy may result from long-distance connections between human groups over the western Late Epigravettian territory.

A review of flame aerosol synthesis technology for the synthesis of nanoparticles and functional energy materials

The outstanding performance of nanomaterials in chemical, magnetic, electrical, catalytic, and mechanical properties has paved the way for the huge market in material fabrication, energy storage, electronics, and many other industries. Traditional synthesis methods are relatively stunted in industrial applicability and scalability due to complex manufacturing processes and long preparation times, hampering the development and commercialisation of nanomaterials. On the other hand, the flame synthesis method emerges as an inexpensive, efficient, and easily scalable method for the commercial production of nanoparticles. The present review aims to highlight the research status and applications of the nanomaterials synthesised by the flame aerosol method. The advancement of flame aerosol synthesis technologies is reviewed, with emphasis on the state-of-the-art flame reactor configuration and design. Critical flame parameters that govern the formation of nanoparticles in the flame are reviewed to provide an understanding of the formation criteria and growth of nanomaterials in the flame environment. The properties and characteristics of carbon-based, platinum group metal, metal oxide, bimetallic nanoparticles, perovskite and high entropy oxide nanomaterials produced by flame synthesis are extensively reviewed. In addition, commercial manufacturing of flame-synthesised materials along with applications of the nanomaterials in the field of thermal or photocatalytic energy storage, fuel cells, and gas sensing are presented.

Enhancing catalytic activity of TiO2 nanoparticles through acid treatment in Eosin-Y sensitized photohydrogen evolution reaction system

Light-driven water splitting has gained increasing attention as an eco-friendly method for hydrogen production. There is a pressing need to enhance the performance of catalysts for the commercial viability of this reaction. Many methods have been proposed to improve catalyst performance; however, an economical and straightforward approach remains a priority. This paper presents an uncomplicated technique called acid treatment, which augments the catalytic performance of nanoparticles. The method promotes a change in the catalytic reactivity by causing a deficit in electron density of Ti and O on the surface of TiO2 nanoparticles without altering their size, morphology, or crystal structure. In the Eosin Y sensitized photocatalytic hydrogen production system, nitric acid treated TiO2 (16.95 μmol/g) exhibited 1.5 times the hydrogen production compared to bare TiO2 (11.15 μmol/g).

Binding of coagulation factor IXa to procoagulant platelets revisited: Low affinity and interactions with other factors

Binding of activated factor IX (fIXa) to the phosphatidylserine-expressing procoagulant platelets is a critical step in blood coagulation, which is necessary for the membrane-dependent intrinsic tenase complex assembly and factor X activation. However, the nature and parameters of the fIXa binding sites on the procoagulant platelet surface remain unclear. We used flow cytometry to elucidate the quantitative details of the fluorescently labeled fIXa binding to gel-filtered activated platelets. FIXa bound to the procoagulant platelet subpopulation only, with the parameters (maximal number of binding sites at 58900 ± 3400, Kd at 1000 ± 170 nM) similar to binding observed with phospholipid vesicles. No specific high-affinity binding sites for fIXa were detected, and binding proceeded similarly for different methods of procoagulant platelet production (thrombin, thrombin receptor activation peptide, collagen-related peptide, their combinations, or calcium ionophore A23187). Factor VIII, known to form a high affinity complex with fIXa, enhanced fIXa binding to platelets. In contrast, only competition effects were observed for factor X, which binds fIXa with much lower affinity. Unexpectedly, fIXa itself, fIX, and prothrombin also dose-dependently enhance fIXa binding at concentrations below 1000 nM, suggesting the formation of membrane-bound fIXa dimers and fIXa-prothrombin complexes on platelets. These findings provide a novel perspective on the fIXa binding site on procoagulant platelets, which does not have any major differences from pure phospholipid-based model membranes, exhibits inherently low affinity (3–5 orders of magnitude below the physiologically relevant fIXa concentration) but is significantly enhanced by its cofactor VIII, and regulated by previously unknown membrane interactions.

Experimental Investigation of PEM Water Electrolyser Stack Performance Under Dynamic Operation Conditions

Water electrolysis has been used to produce green hydrogen, for which identifying optimum operation parameters is crucial to improve its energy efficiency and energy consumption. This paper used a commercial proton exchange membrane (PEM) water electrolyser stack (180 W) to demonstrate the correlation between operating current change, temperature, and water flow rate and their impact on the thermal and electrical performance of the stack. It was found that the current control regime and temperature control can offset the voltage ageing in a long-term operating electrolyser with no negative impact on the H2 production rate. For a controlled decreasing current path, in the medium range of operating current, the stack’s energy efficiency was improved by 5%, and 3.7% specific energy consumption can be saved comparing to the standard operation (57.8 kWh·kg−1H2). The results provide insights into the potential optimisation in operation conditions to further increase cell energy efficiency and reduce energy consumption. This new finding sheds light on developing an energy- and cost-saving operating method for long-term green hydrogen production via water electrolysis.

Rapid production of abundant transgenic pomegranate (Punica granatum) hairy roots

Pomegranates (Punica granatum) are known for their high levels of health-beneficial compounds that belong to the hydrolyzable tannin (HT) and flavonoid families. However, a significant gap in our understanding exists of the biosynthetic and regulatory genes related to the accumulation of these compounds. To this end, the induction and transformation of hairy roots presents an exciting opportunity to decipher genetically the functions of candidate genes involved in the HT and flavonoid metabolism. This study aims at improving methods for rapid and abundant hairy root production from pomegranate explants by increasing seed germination rates and expediting the attainment of requisite biomass for multifaceted analyses. We found that treating seeds with sandpaper and concentrated sulfuric acid significantly enhanced their germination rates. Interestingly, no correlation between seed-coat texture and seed germination rate was observed among the 20 pomegranate accessions evaluated in this study. Notably, transferring hairy roots from agar plates to soil promoted rapid root biomass growth compared to maintaining them on agar plates. Root biomass as well as levels of gallic acid (a precursor for HT biosynthesis) and punicalagins (the major HTs in roots) across 15 pomegranate accessions were also analyzed. Three of these accessions exhibited higher seed germination percentages along with augmented biomass and elevated punicalagin levels. These pomegranate accessions emerge as promising germplasm for future genetic transformation and functional genomics studies.