Culture Production Research Articles

Research culture and scientific production in postgraduate teachers

La pandemia obligo a los profesores asumir nuevas habilidades en investigación que le permita aportar a la cultura de investigación, este estudio tuvo como propósito explicar la relación entre cultura investigativa y producción científica en docentes de posgrado en una universidad nacional. De nivel descriptivo, correlacional, de método hipotético- deductivo, de enfoque cuantitativo, para lo cual se estudió una muestra de 60 docentes, se utilizó la encuesta y el cuestionario como instrumento de recolección de datos. Se concluyó la relación positiva y directa de ,839 (significancia = ,000˂,050) entre cultura investigativa y producción científica en docentes de posgrado en una universidad nacional, lo que indicó que los procesos dentro de la promoción de la cultura investigativa en la institución al ser mejorados traerán consigo una mayor producción científica.

ACTH and prolactin synergistically and selectively regulate CYP17 expression and adrenal androgen production in human foetal adrenal organ cultures.

The essential role of ACTH on the growth and function of the human foetal adrenal (HFA) has long been recognized. In addition, many studies have suggested a role of the pituitary hormone prolactin (PRL) in the regulation of the HFA, but the effects of this hormone on steroidogenesis and gene expression are still unknown. Our objective was to investigate the effect of ACTH and PRL on the steroidogenic capacities of the HFA. In vitro/ex vivo experimental study. We used a hanging drop in vitro organ culture system. First trimester HFA samples were cultured for 14 days in basal conditions or treated with ACTH, PRL, or a combination of the 2 (3 to 11 replicates depending on the experiment). Steroids were measured by liquid chromatography/tandem mass spectrometry or immunoassay, gene expression by RT-qPCR, and protein expression by immunoblot. ACTH significantly increased corticosterone, cortisol, and cortisone production, both by itself and when used together with PRL. PRL stimulation by itself had no effect. Combined stimulation with ACTH + PRL synergistically and selectively increased adrenal androgen (DHEAS and Δ4-androstenedione) production and CYP17A1 expression in the HFA, while treatment with each single hormone had no significant effect on those steroids. These results have important implications for our understanding of the hormonal cues regulating adrenal steroidogenesis in the HFA during the first trimester in physiological and pathological conditions and warrant further studies to characterize the molecular mechanisms of converging ACTH and PRL signalling to regulate CYP17A1 expression.

Mechanism-based inhibition of gut microbial tryptophanases reduces serum indoxyl sulfate

Indoxyl sulfate is a microbially derived uremic toxin that accumulates in late-stage chronic kidney disease and contributes to both renal and cardiovascular toxicity. Indoxyl sulfate is generated by the metabolism of indole, a compound created solely by gut microbial tryptophanases. Here, we characterize the landscape of tryptophanase enzymes in the human gut microbiome and find remarkable structural and functional similarities across diverse taxa. We leverage this homology through a medicinal chemistry campaign to create a potent pan-inhibitor, (3S) ALG-05, and validate its action as a transition-state analog. (3S) ALG-05 successfully reduces indole production in microbial culture and displays minimal toxicity against microbial and mammalian cells. Mice treated with (3S) ALG-05 show reduced cecal indole and serum indoxyl sulfate levels with minimal changes in other tryptophan-metabolizing pathways. These studies present a non-bactericidal pan-inhibitor of gut microbial tryptophanases with potential promise for reducing indoxyl sulfate in chronic kidney disease.

The Optimization, Kinetics Model, and Lab-Scale Assessments of Phenol Biodegradation Using Batch and Continuous Culture Systems

Phenol was considered a severe hazard to all ecosystems even at low concentrations. The bioremediation process is an eco-friendly process for complete phenol degradation and bioelectricity generation. In the present study, a consortium of native isolates was used for phenol biodegradation and bioenergy generation using nano-graphite electrodes. The optimization of nutritional and environmental parameters using batch culture revealed that the optimum conditions for maximum phenol degradation and energy generation were inoculum concentration, 1%; incubation period, 48 h; phenol, 6 ppm; MgSO4, 70 mg/L; K2HPO4, 175 mg/L; and CaCl2, 1 mg/L. Phenol biodegradation reached 93.34% with a power density of 109.419 mW/cm3. A lab-scale bioreactor was used as a continuous culture with aeration rate, agitation speed, and dissolved oxygen of 0.5 v/v/m, 750 rpm, and 30%, respectively. On using the continuous culture, phenol biodegradation and bioenergy production reached 97.8% and 0.382 W/cm3, respectively. A kinetics study using Haldane’s kinetics model reported the best fit to achieve a significant correlation coefficient (R2) value (0.9865) reaching maximum specific growth rate with initial phenol concentration of approximately 9 mg L−1 where the specific growth rates (μ, h−1) varied with different initial phenol concentrations. In conclusion, the native isolated consortium could be considered as an economical and sustainable approach to phenol biodegradation in industrial wastewater as well as bioelectricity generation.

Effects of light quality and intensity on growth and bromoform content of the red seaweed Asparagopsis taxiformis

AbstractSpecies of the genus Asparagopsis are rich in halogenated bioactive compounds, particularly bromoform. Its use as a feed additive in ruminant livestock drastically decreases the animal’s methane production, thereby reducing the industry’s environmental impact. Addressing the high demand for Asparagopsis biomass requires the understanding of the culture conditions that promote higher growth rates and bromoform content. Here we evaluated how different light quality combinations (High-Blue:Red, Medium Blue:Red, High-Blue:Green:Red, and White) and four light intensities (30, 60, 90 and 120 μmol photons m−2 s−1) affect the growth and bromoform content of the Asparagopsis taxiformis tetrasporophyte in indoor tumbling cultures at two biomass densities. We also assessed the effect of light intensity on the photosynthetic response by measuring oxygen evolution rates. Light spectra containing intermediate wavelengths promoted higher growth, regardless of biomass density. Of the different light qualities tested, white light promoted the highest bromoform content. Increasing light intensity led to a positive response in A. taxiformis growth. However, the photosynthetic parameters estimated showed that the two higher light intensity treatments were above the saturation irradiance, for both culture densities. This, along with the observed development of contamination, suggests that long-term cultures of A. taxiformis should be maintained at light intensities no higher than 60 μmol photons m−2 s−1. In addition, we found that exposing cultures to higher irradiances does not guarantee a bromoform-richer biomass. These results offer valuable insights for optimizing biomass and bioactive compound production in indoor cultures of the Asparagopsis genus.

Antimycobacterial and nitric oxide production inhibitory activities of limonoids isolated from Trichilia lepidota subsp. schumanniana (Harms) T.D.Penn

Six previously undescribed intact limonoids together with four known compounds were isolated from the seeds of Trichilia lepidota subsp. schumanniana (Harms) T.D.Penn. Their structures were characterized based on one- and two-dimensional nuclear magnetic resonance spectra, infrared, ultraviolet, mass spectroscopy results, and optical rotation. All compounds were evaluated for their ability to inhibit nitric oxide production in cultures of RAW 264.7 macrophages stimulated by lipopolysaccharide, cytotoxicity and growth of Mycobacterium tuberculosis strains H37Rv and M299. The compounds 7-deacetyl-11β,12α-diacetoxy-14,15-epoxyazadirone (5) and walsurin E (9) were the most potent in inhibiting nitric oxide production, although the compounds 1-deshydroxy-12α-acetoxymunronin N (1) and 6α,12α-dihydroxyazadirone (6) also showed controlled potential of this mediator, in addition to being potent growth inhibitors of Mycobacterium tuberculosis H37RV and M299, without cytotoxicity interference. Ring intact limonoids isolated from Trichilia lepidota subsp. schumanniana seeds are a new source of bioactive substances that may be used in the future against diseases such as tuberculosis and other processes related to inflammation.

Regulation of serotonin production by specific microbes from piglet gut

BackgroundSerotonin is an important signaling molecule that regulates secretory and sensory functions in the gut. Gut microbiota has been demonstrated to affect serotonin synthesis in rodent models. However, how gut microbes regulate intestinal serotonin production in piglets remains vague. To investigate the relationship between microbiota and serotonin specifically in the colon, microbial composition and serotonin concentration were analyzed in ileum-cannulated piglets subjected to antibiotic infusion from the ileum when comparing with saline infusion. Microbes that correlated positively with serotonin production were isolated from piglet colon and were further used to investigate the regulation mechanisms on serotonin production in IPEC-J2 and a putative enterochromaffin cell line RIN-14B cells.ResultsAntibiotic infusion increased quantities of Lactobacillus amylovorus (LA) that positively correlated with increased serotonin concentrations in the colon, while no effects observed for Limosilactobacillus reuteri (LR). To understand how microbes regulate serotonin, representative strains of LA, LR, and Streptococcus alactolyticus (SA, enriched in feces from prior observation) were selected for cell culture studies. Compared to the control group, LA, LR and SA supernatants significantly up-regulated tryptophan hydroxylase 1 (TPH1) expression and promoted serotonin production in IPEC-J2 cells, while in RIN-14B cells only LA exerted similar action. To investigate potential mechanisms mediated by microbe-derived molecules, microbial metabolites including lactate, acetate, glutamine, and γ-aminobutyric acid were selected for cell treatment based on computational and metabolite profiling in bacterial supernatant. Among these metabolites, acetate upregulated the expression of free fatty acid receptor 3 and TPH1 while downregulated indoleamine 2,3-dioxygenase 1. Similar effects were also recapitulated when treating the cells with AR420626, an agonist targeting free fatty acid receptor 3.ConclusionsOverall, these results suggest that Lactobacillus amylovorus showed a positive correlation with serotonin production in the pig gut and exhibited a remarkable ability to regulate serotonin production in cell cultures. These findings provide evidence that microbial metabolites mediate the dialogue between microbes and host, which reveals a potential approach using microbial manipulation to regulate intestinal serotonin biosynthesis.

Continuous cultivation of microalgae yields high nutrient recovery from nitrified urine with limited supplementation

Microalgae can play a key role in the bioeconomy, particularly in combination with the valorisation of waste streams as cultivation media. Urine is an example of a widely available nutrient-rich waste stream, and alkaline stabilization and subsequent full nitrification in a bioreactor yields a stable nitrate-rich solution. In this study, such nitrified urine served as a culture medium for the edible microalga Limnospira indica. In batch cultivation, nitrified urine without additional supplements yielded a lower biomass concentration, nutrient uptake and protein content compared to modified Zarrouk medium, as standard medium. To enhance the nitrogen uptake efficiency and biomass production, nitrified urine was supplemented with potentially limiting elements. Limited amounts of phosphorus (36 mg L−1), magnesium (7.9 mg L−1), calcium (12.2 mg L−1), iron (2.0 mg L−1) and EDTA (88.5 mg Na2-EDTA.2H2O L−1) rendered the nitrified urine matrix as effective as modified Zarrouk medium in terms of biomass production (OD750 of 1.2), nutrient uptake (130 mg N L−1) and protein yield (47%) in batch culture. Urine precipitates formed by alkalinisation could in principle supply enough phosphorus, calcium and magnesium, requiring only external addition of iron, EDTA and inorganic carbon. Subsequently, the suitability of supplemented nitrified urine as a culture medium was confirmed in continuous Limnospira cultivation in a CSTR photobioreactor. This qualifies nitrified urine as a valuable and sustainable microalgae growth medium, thereby creating novel nutrient loops on Earth and in Space, i.e., in regenerative life support systems for human deep-space missions.

CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells

MDCK is currently the main cell line used for influenza vaccine production in culture. Previous studies have reported that MDCK cells possess tumorigenic ability in nude mice. Although complete cell lysis can be ensured during vaccine production, host cell DNA released after cell lysis may still pose a risk for tumorigenesis. Greater caution is needed in the production of human vaccines; therefore, the use of gene editing to establish cells incapable of forming tumors may significantly improve the safety of influenza vaccines. Knowledge regarding the genes and molecular mechanisms that affect the tumorigenic ability of MDCK cells is crucial; however, our understanding remains superficial. Through monoclonal cell screening, we previously obtained a cell line, CL23, that possesses significantly reduced cell proliferation, migration, and invasion abilities, and tumor-bearing experiments in nude mice showed the absence of tumorigenic cells. With a view to exploring tumorigenesis-related genes in MDCK cells, DIA proteomics was used to compare the differences in protein expression between wild-type (M60) and non-tumorigenic (CL23) cells. Differentially expressed proteins were verified at the mRNA level by RT-qPCR, and a number of genes involved in cell tumorigenesis were preliminarily screened. Immunoblotting further confirmed that related protein expression was significantly reduced in non-tumorigenic cells. Inhibition of CDC20 expression by RNAi significantly reduced the proliferation and migration of MDCK cells and increased the proliferation of the influenza virus; therefore, CDC20 was preliminarily determined to be an effective target gene for the inhibition of cell tumorigenicity. These results contribute to a more comprehensive understanding of the mechanism underlying cell tumorigenesis and provide a basis for the establishment of target gene screening in genetically engineered non-tumorigenic MDCK cell lines.

Transmission and Development of Food Heritage under Rural Revitalization: Yongfeng chili sauce in China

In the context of China's rural revitalization strategy, this study explores the transmission and development of food heritage, using the case of Yongfeng Chili Sauce as a reference for local governments to formulate intangible cultural heritage protection policies. Qualitative methods were employed to gather data from family workshops and factories. Narrative analysis was conducted on data obtained from observations and interviews through fieldwork, addressing the research questions. Findings indicate, Yongfeng chili sauce carries rich culture and unique production techniques; is one of the pillar industries of the local economy; and significantly promotes rural revitalization if the local government's current concern.

DETERMINATION OF THE EFFECTS OF DIFFERENT PROTOCOLS ON TISSUE CULTURE MICROPROPRIATION IN EUPHORBIA MILII

In recent years, with the emergence of some important species in the field of ornamental plants, the interest in some species naturally grown in nature has started to increase and the Euphorbia milii species has started to create related demands both in commercial and hobby terms. In order to meet these demands, seedlings produced by appropriate methods are needed. Clonal propagation is very important in Euphorbia milii as in other fruit species. Tissue culture techniques are the leading methods in clonal propagation. Although there are various studies on production by tissue culture technique in Euphorbia milii species, it is known that different protocols used in tissue culture have different micropropagation efficiency. However, when commercial production is started, determining the most effective protocol and increasing the micropropagation efficiency will contribute to the reduction of both time and inputs. Studies on in vitro micropropagation have been carried out in Euphorbia milii plant and successful results have been obtained in each study. However, it is still not known exactly which protocol is more successful in the materials to be studied. From this point of view, in this study, it was aimed to determine the effects of different protocols on the micropropagation efficiency of Euphorbia milii plants. In present study, 3 different plant tissue culture protocol were used and, as obtained results, although plantlets were obtained from 3 protocols, the most successful protocol was the P1 protocol. The obtained findings are of a quality that can contribute to tissue culture production of Euphorbia milii plant and other scientific studies.

Effect of mixtures of legume species on ruminal fermentation, methane, and microbial nitrogen production in batch and continuous culture (RUSITEC) systems

The effect of cicer milkvetch ( Astragalus cicer L.) and sainfoin ( Onobrychis viciifolia Scop.) on ruminal fermentation, methane production, and microbial nitrogen synthesis was assessed in two experiments. Experiment 1 analyzed two legumes, cicer milkvetch and sainfoin at two stages (vegetative and late flower) incubated with alfalfa ( Medicago sativa) at five inclusion rates 0:100; 25:75, 50:50, 75:25, and 100:0 (as DM) in batch culture. Experiment 2 analyzed vegetative cicer milkvetch and alfalfa incubated in ratios of 25:75, 50:50, 75:25, and 100:0 (as DM) in continuous culture systems (RUSITEC). In batch culture, increased dry matter disappearance (DMD), and propionate percentage (%total), and reduced methane (mg·g−1 DMD) occurred with vegetative cicer milkvetch inclusion. In RUSITEC, DMD linearly increased ( P < 0.01), acetate:propionate ratio quadratically decreased, while ammonia (NH3-N) concentration ( P < 0.01) and butyrate percentage (%total) linearly decreased ( P < 0.05) with increasing inclusion of cicer milkvetch. No differences were observed for methane (CH4) production (mg·g−1 DMD), or short chain fatty acid (SCFA) production (mmol·day−1). Microbial nitrogen synthesis and efficiency of protein synthesis linearly increased ( P < 0.05) with increased inclusion of cicer milkvetch. Results suggest cicer milkvetch may result in synchronicity of energy and nitrogen during rumen fermentation, which could enhance cattle production.

Antimicrobial and teat sealant use and selection criteria at dry-off on Canadian dairy farms

Infections with antimicrobial resistant pathogens are a major threat to human and animal health worldwide. Further, reduction of livestock-associated antimicrobial use (AMU) is often identified as an area of focus. Selective dry cow therapy (DCT) warrants consideration as an important way to decrease AMU on Canadian dairy farms. In addition, teat sealants (TS) are a nonantimicrobial alternative for prevention of intramammary infection during the dry period. Therefore, objectives of this study were to determine how antimicrobials and TS are used at dry-off on Canadian dairy farms to determine selective DCT uptake and enacted selection protocols. It was expected that these data will provide a baseline understanding of DCT practices and highlight areas for future intervention to further reduce AMU. An observational study was conducted utilizing 2 in-person questionnaires conducted between July 2019 and September 2021 on 144 participating dairy farms in 5 Canadian provinces (British Columbia = 30, Alberta = 30, Ontario = 31, Québec = 29, and Nova Scotia = 24). Overall, 45 farms (31%) reported adopting selective DCT, 95 (66%) enacted blanket DCT, and 4 (3%) did not provide antimicrobial DCT. Farms enacting selective DCT had approximately 50% less intramammary antimicrobials used at dry-off compared with blanket DCT farms. Cow somatic cell count history was the most common criterion for selective DCT decision-making, followed by previous clinical mastitis history, bacteriological culture, and milk production. A slight majority of farms (56%) applied TS to all cows at dry-off, whereas 17 farms (12%) used TS selectively, and 46 farms (32%) did not use TS. Larger herds more often used TS, and farms with an automatic milking system more often used TS selectively than applied to all cows. Results highlighted the variability in antimicrobial treatment and TS use protocols at drying off on Canadian dairy farms, and the potential for further antimicrobial reduction with increased adoption of selective DCT.

Distinct CRTH2+CD161+ (peTh2) memory CD4+ T-cell cytokine profiles in food allergy and eosinophilic gastrointestinal disorders.

Although IgE-mediated food allergy (FA) and eosinophilic gastrointestinal disorders (EGID) are clinically distinct and treated differently, pathogenic effector Th2 (peTh2) cells are implicated in the pathogenesis of both FA and EGID. The aim of this study was to better characterize peTh2 cells in the context of FA and EGID and the overlap between these two conditions. Peripheral blood peTh2 cells (CD3+CD4+CD27-CD49d+CRTH2+CD161+) were profiled by intracellular cytokine flow cytometry in the following patient cohorts: patients with FA alone (n = 8), FA and food-triggered EGID (EGID+FA+FT, n = 7), food-triggered EGID alone (EGID+FT, n = 7), EGID without FA or specific food triggers (ONLY_EGID, n = 9), and healthy volunteers (HV, n = 7). Overnight peripheral blood mononuclear cell (PBMC) culture supernatants were assessed for cytokine production by multiplex analysis. CRTH2+CD161+ (peTh2) memory CD4+ T cells were significantly increased in both patients with FA and those with ALL_EGID (inclusive of EGID+FA+FT, EGID+FT and ONLY_EGID) when compared to HV. However, ALL_EGID patients, particularly those with EGID+FA+FT, had significantly elevated IL-5+IL-13+ peTh2 cells, whereas FA patients had significantly elevated IFN-γ or IL-17A-expressing peTh2 cells. This finding was supported by increased spontaneous IL-5 and IL-13 production in overnight cultures of PBMC from EGID+FA+FT patients compared to spontaneous IL-10 and IFN-γ production by PBMC from FA patients. FA patients had increased IL-9, IL-10, IL-17A, and IFN-γ production in overnight cultures of stimulated PBMC. EGID and IgE-mediated FA share a common cell subtype defined by specific surface markers and termed CRTH2+CD161+ (peTh2) memory CD4+ T cells. However, the cytokine profiles of these CRTH2+CD161+ (peTh2) memory CD4+ T cells are markedly different between the two disorders.

CULTURA CORPORAL E EDUCAÇÃO FÍSICA A PARTIR DA FILOSOFIA MARXISTA

In the generation of knowledge, especially in the field of Physical Education, there is a historical conflict in Brazil between two theoretical-pedagogical and epistemological views. In this study, the focus is given to body culture, contrasting with the vision of physiotherapy in the area of School Physical Education in Brazil. The objective is to highlight the importance of knowledge of body culture as a result of work, in its intangible and essential dimension for human existence, as a determining factor in the cultural expression of the body, going beyond the emphasis on physiotherapy. In this sense, the theoretical-methodological basis was based on the principles of Marxist philosophy, in the context of Historical Materialism and the Dialectical method. Finally, we emphasize the sign of body culture as a component of culture and human production, which shapes the expression of the body of individuals, through the organization of body activities, expansion of the cultural heritage of humanity and overcoming the forms of body expression inspired by the idea of physics.

Proapoptotic effects of halogenated bis-phenylthiourea derivatives in cancer cells.

New halogenated thiourea derivatives were synthesized via the reaction of substituted phenylisothiocyanates with aromatic amines. Their cytotoxic activity was examined in in vitro studies against solid tumors (SW480, SW620, PC3), a hematological malignance (K-562), and normal keratinocytes (HaCaT). Most of the compounds were more effective against SW480 (1a, 3a, 3b, 5j), K-562 (2b, 3a, 4a), or PC3 (5d) cells than cisplatin, with favorable selectivity. Their anticancer mechanisms were studied by Annexin V-fluorescein-5-isothiocyanate apoptosis, caspase-3/caspase-7 assessment, cell cycle analysis,interleukin-6 (IL-6) release inhibition, and reactive oxygen species(ROS) generation assay. Thioureas 1a, 2b, 3a, and4a were the most potent activators of early apoptosis in K-562 cells, and substances 1a, 3b, 5j triggered late-apoptosis or necrosis in SW480 cells. This proapoptotic effect was proved by the significant increase of caspase-3/caspase-7 activation. Cell cycle analysis revealed that derivatives 1a, 3a, 5j increased the number of SW480 and K-562 cells in the sub-G1 and/or G0/G1 phases, and one evoked cycle arrest at the G2 phase. The most potent thioureas inhibited IL-6 cytokine secretion from PC3 cells and both colon cancer cell lines. Apoptosis-inducing compounds also increased ROS production in all tumor cell cultures, which may enhance their anticancer properties.

Quantification and FTIR Analysis of Precursor Mediated Alkaloid Production in Cell Suspension Culture of Gnidia glauca (Fresen.) Gilg

The current investigation focuses on developing a reproducible protocol for callus induction and for studying the precursor L-phenylalanine impact on product enhancement for alkaloid production and measurement of pH. Alkaloids are nitrogen-containing compounds; individual alkaloids were purified and synthesized commercially as excellent substances with recognized and experimentally verified pharmacological attributes because of their antimicrobial, anticancer properties and other therapeutic values. The current studies explore the impact of Precursor on alkaloid production by cell suspension culture in G. glauca, resulting in an increase in the cell growth observed and quantified by HPLC, TLC analysis, and the presence of functional groups identified by FTIR profiling. Additionally, this finding offers a protocol for biomass production of alkaloids from medicinally important endangered plants aiding in the conservation of the plants as well as producing commercially valuable secondary metabolites.

ON THE PROBLEM OF SOCIAL RECONSTRUCTIONS BASED ON THE MATERIALS OF THE SETTLEMENTS OF THE HALAF CULTURE OF THE EASTERN EUPHRATES

In the Russian archeology an opinion about the egalitarian appearance of the Neolithic settlements of the Halaf culture in Northern Mesopotamia was established. An assumption about the correspondence of its social structure to the level of chiefdom was made. However, no special studies in this direction have been carried out until now. This article contains the experience of identifying of social information from the archaeological sources of settlements in North-Eastern Syria. Developing the idea of the multicomponent specific of large settlements, the data that the link of social unification was a large family community are showed. Modern knowledge about the settlements allow to suggest that leaders of large-family communities exercised control over the development of specific sections of the settlement. Rare structural elements in housing construction (stone foundation, different qualities of wall plastering, floor covering, stone paving of the yard), which were not in the tradition of the Halaf cultural population, allow to consider them as signs of property and social gradation. In Russian and foreign science, a high level of pottery production in the Halaf culture has been noted. It is noted that the formation of independent production activity began with pottery, whereas in the relatively northern territories of Eurasia, many centuries later, this process began with metallurgy. This constitutes a feature of the ancient history of Mesopotamia.

Effect of silver nanoparticles on tropane alkaloid production of transgenic hairy root cultures of Hyoscyamus muticus L. and their antimicrobial activity

The utilization of nanotechnology and biotechnology for enhancing the synthesis of plant bioactive chemicals is becoming increasingly common. The hairy root culture technique can be used to increase secondary metabolites such as tropane alkaloids. Agrobacterium was used to induce hairy roots from various explants of Hyoscyamus muticus. The effect of nano-silver particles (AgNPs) at concentrations of 0, 25, 50, 100, and 200 mg/L on tropane alkaloids synthesis, particularly hyoscyamine and scopolamine, was studied in transgenic hairy root cultures. Different types of explants obtained from 10-day-old seedlings of H. muticus were inoculated with two strains of Agrobacterium rhizogenes (15,834 and A4). The antimicrobial activity of an ethanolic extract of AgNPs-induced hairy root cultures of H. muticus was tested. The frequency of hairy roots was higher in hypocotyl, root, leaf, and stem explants treated with A. rhizogenes strain A4 compared to those treated with strain 15,834. In transgenic hairy root cultures, AgNPs application at a concentration of 100 mg/L resulted in the highest total tropane alkaloid production, which exhibited broad-spectrum antimicrobial activity. The study demonstrated the potential of nano-silver as an elicitor for promoting the production of target alkaloids in Hyoscyamus muticus hairy root cultures, which exhibit high biological activity.

Novel pilot bioreactor for scale up Gynura procumbens adventitious root culture

Adventitious root culture and the development of a bioreactor are methods to obtain biomass and plant bioactive compounds in large quantities faster than conventional plant cultivation. These technologies provide an excellent opportunity to produce biomass and bioactive compounds from plants, especially Gynura procumbens. Previous reports mentioned a small-scale bioreactor could increase biomass and bioactive compounds of G. procumbens adventitious roots. Therefore, a larger bioreactor for adventitious root culture is necessary to develop. In this study, the development of the 19 L pilot bioreactor was successful. The optimal condition for bioreactor sterilization is 1.8 bar for 60 min using an autoclave. We found that G. procumbens adventitious roots culture in pilot bioreactors has resulted in optimal biomass using MS-Tek media (technical grade) compared to MS-PA (pro analyze) media after 35 d of the culture period. Although, higher productivity of total phenolics and total flavonoids in G. procumbens roots has been achieved from MS-PA media rather than MS-Tek media. In further study, it is necessary to evaluate the effect of technical-grade chemicals on kinetics root growth and chemical uptake. The hope is to obtain a suitable media formulation with affordable cost for adventitious root culture production.