Abstract
The current investigation focuses on developing a reproducible protocol for callus induction and for studying the precursor L-phenylalanine impact on product enhancement for alkaloid production and measurement of pH. Alkaloids are nitrogen-containing compounds; individual alkaloids were purified and synthesized commercially as excellent substances with recognized and experimentally verified pharmacological attributes because of their antimicrobial, anticancer properties and other therapeutic values. The current studies explore the impact of Precursor on alkaloid production by cell suspension culture in G. glauca, resulting in an increase in the cell growth observed and quantified by HPLC, TLC analysis, and the presence of functional groups identified by FTIR profiling. Additionally, this finding offers a protocol for biomass production of alkaloids from medicinally important endangered plants aiding in the conservation of the plants as well as producing commercially valuable secondary metabolites.
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