Image analysis as a technique for fragmentation measurement of rock piles has been the subject of research since the 1980s, and to date, run of mine (ROM) fragmentation optimisation studies have primarily relied on particle size measurement using photographic-based 2D imaging systems. Disadvantages of 2D imaging systems include particle delineation errors because of variable lighting and material colour and texture variation; no direct measure of scale and perspective distortion; and inability to distinguish overlapped particles, non-overlapped particles, and areas of fines. With the development of 3D imaging technologies, there is an opportunity to develop techniques that could improve data collection and overcome the limitations of existing 2D image-based systems. This paper describes the first attempt to use 3D high-resolution laser scanning techniques to quantify ‘whole of muckpile’ fragmentation from full-scale production blasting. During two monitoring campaigns in 2013, high-resolution laser scanning data were collected from production blasts at Esperanza mine (Antofagasta Minerals Group). Fully automated analysis of the 3D data was possible in all cases where the data were of sufficiently high resolution. Manual pre-processing was required when the data were of low resolution to specify the region of fines. Overall results indicated that ROM fragmentation requirements were meeting specified targets, despite the marked differences in powder factors. This was particularly the case for those blasts conducted in similar geological domains. This work has demonstrated that high-resolution laser scanning can be used as an alternative technique to measure ‘whole of muckpile’ fragmentation in production blasting.