Ozonated ice not only retains the inherent performance and function of ozone, but also serves as a cold source for food preservation. However, its preparation is challenging due to the low solubility and instability of ozone in water. So here, the major preparation techniques and the storage stability of ozonated water and ice were studied. Besides, the impact of ozonated ice treatment on the quality of shrimp (Metapenaeus ensis) was explored. To achieve these ends, a set of ozonated ice preparation devices was established, and the optimal conditions for producing ozonated water and ice were confirmed as follows: using 0 °C deionized water as the water source, achieving an outlet flow rate of 80 L/h, cyclically mixing gas-liquid twice, adding 1.0 g/kg of polyoxypropylene oxyethylene glycol ether (POGE), and freezing at −80 °C. Regarding ozone stability, an increase in initial ozone concentration and storage temperature, along with a decrease in water purity, resulted in a shorter half-life of ozone in water. Additionally, a lower storage temperature was conducive to prolonging the half-life of ozonated ice, while the addition of POGE had little effect. In practical application, under the optimal conditions for producing ozonated water and ice without POGE, shrimp stored in ozonated ice demonstrated superior sensory scores, whiteness values, and shear force compared to those stored in normal ice. Concurrently, they displayed reduced levels of TVC, TVB-N, and pH. Furthermore, the application of ozonated ice treatment mitigated blackening and improved odor quality. Consequently, this research offers a scientific foundation for the industrial application of ozonated water and ice and serves as a reference for preventing the deterioration and quality loss of shrimp and other seafood products.
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