Production Capability Research Articles

Enhanced ER protein processing gene expression increases rAAV yield and full capsid ratio in HEK293 cells

The recombinant adeno-associated virus (rAAV) vector is among the most promising viral vectors in gene therapy. However, the limited manufacturing capacity in human embryonic kidney (HEK) cells is a barrier to rAAV commercialization. We investigated the impact of endoplasmic reticulum (ER) protein processing and apoptotic genes on transient rAAV production in HEK293 cells. We selected four candidate genes based on prior transcriptomic studies: XBP1, GADD34 / PPP1R15A, HSPA6, and BCL2. These genes were stably integrated into HEK293 host cells. Traditional triple-plasmid transient transfection was used to assess the vector production capability and the quality of both the overexpressed stable pools and the parental cells. We show that the overexpression of XBP1, HSPA6, and GADD34 increases rAAV productivity by up to 100% and increases specific rAAV productivity by up to 78% in HEK293T cells. Additionally, more prominent improvement associated with ER protein processing gene overexpression was observed when parental cell productivity was high, but no substantial variation was detected under low-producing conditions. We also confirmed genome titer improvement across different serotypes (AAV2 and AAV8) and different cell lines (HEK293T and HEK293); however, the extent of improvement may vary. This study unveiled the importance of ER protein processing pathways in viral particle synthesis, capsid assembly, and vector production.Key points• Upregulation of endoplasmic reticulum (ER) protein processing (XBP1, HSPA6, and GADD34) leads to a maximum 100% increase in rAAV productivity and a maximum 78% boost in specific rAAV productivity in HEK293T cells• The enhancement in productivity can be validated across different HEK293 cell lines and can be used for the production of various AAV serotypes, although the extent of the enhancement might vary slightly• The more pronounced improvements linked to overexpressing ER protein processing genes were observed when parental cell productivity was high, with minimal variation noted under low-producing conditions

A preliminary investigation into the potential of Ge-enhanced Cu2SnS3 (CTS) thin-film applications for water-splitting photoelectrodes

This study explores the potential of Ge-enhanced Cu2SnS3 (CTS) thin-films as photoelectrode materials for water splitting grown through a simple sulfurization process. The addition of Ge to CTS enabled tuning the bandgap and improved the photocurrent density. Films sulfurized at 520 °C exhibit enhanced grain size and reduced grain boundaries, which contribute to increased carrier transport efficiency. By optimizing Ge content and sulfurization conditions, the Cu2(Sn1−x ,Ge x )S3 films demonstrate promising capabilities for efficient green hydrogen production. This work lays the groundwork for developing advanced photoelectrodes and highlights the need for further refinement to maximize performance for practical applications.

Evaluation of tritium production capability of the 5 MWe gas-cooled graphite-moderated reactor at Yongbyon using tritium-producing burnable absorber rods

The 5 MWe graphite-moderated gas-cooled reactor located at Yongbyon, North Korea, is a major facility for producing nuclear materials. The objective of this study is to evaluate the tritium production capability of the 5 MWe reactor when using a tritium-producing burnable absorber rod (TPBAR). This paper presents burnup analysis results for TPBAR-embedded core models performed by McCARD, a Monte Carlo neutron transport simulation code. For three types of cores with TPBARs, the numerical results show that the 5 MWe reactor can produce 1.83–7.51 g of tritium per year depending on the number of TPBARs in the core. The numerical results can serve as a basis for assessing the feasibility of producing tritium using graphite-moderated gas-cooled reactors.

Microstructural Analysis and Preliminary Wear Assessment of Wire Arc Additive Manufactured AA 5083 Aluminum Alloy for Lightweight Structures

The proliferation of Wire Arc Additive Manufacturing (WAAM) has significantly enhanced the production capabilities for lightweight and structurally robust components. This study investigates the microstructural characteristics, tensile properties, and preliminary wear performance of AA 5083 aluminum alloy processed via WAAM, focusing on applications for lightweight structures. Using SEM and XRD, microstructural changes during the WAAM process are analyzed, and tensile testing evaluates the mechanical properties, including ultimate tensile strength (UTS) and elongation. The results reveal that the microstructure consists of α-Al and β-(Al5Mg8) phases, with the Al5Mg8 phase distributed along grain boundaries and within grains. Notably, the grain size in the Y-direction (building direction) is larger than in the X-direction (deposition direction) due to temperature variations during processing. Tensile testing shows that horizontal samples (X-direction) have a UTS of 295 ± 5 MPa and elongation of 20.08 ± 0.8%, while vertical samples (Y-direction) have a UTS of 267 ± 10 MPa and elongation of 16.43 ± 2.1%. This results in an anisotropy of 9.4% in tensile strength, reflecting the differences in mechanical properties between the two directions. The WAAM AA 5083 aluminum part exhibits a maximum wear rate of 5.22 × 10⁻³ mm³/m and a coefficient of friction of 0.52 at a load of 3.5 kg and 450 rpm. Under these conditions, deep grooves, layer separation, and load-induced deformation are observed. The primary wear mechanisms include delamination, adhesion, and abrasion. Hardness levels are consistent in the X-direction and show minimal variance in the Y-direction, with an average hardness of 89.4 ± 5.14 HV0.5. The study demonstrates that WAAM-produced AA 5083 aluminum alloy, with an anisotropy below 10%, is suitable for real-time lightweight structures, offering effective performance in engineering applications such as aerospace and automotive industries. Future research should focus on further quantifying wear behavior and optimizing processing conditions to enhance material performance for specific applications.

BODIPY‐based iridium and ruthenium complexes: Synthesis, photophysical, and photochemical properties

AbstractThe design, synthesis, and characterization of novel BODPY‐based iridium and ruthenium complexes were reported. The structures of new compounds were fully characterized by FT‐IR, MALDI‐TOF mass analysis, elemental analysis, 1H, and 19F NMR spectroscopies. Precursor BODIPY was characterized by single‐crystal x‐ray diffraction technique for the first time. Photophysical properties including absorption and emission profiles and lifetimes were investigated via UV–vis absorption and emission spectroscopy. Photochemical properties were investigated via direct method, and the singlet oxygen production capabilities of new complexes were determined by NIR phosphorescence methods. BODIPY‐based new complexes were efficient at producing 1O2. Besides, both complexes showed more remarkable photosensitization ability than some commonly used photosensitizer based on BODIPY derivatives. This study points out that novel complexes are effective 1O2 photosensitizers that might be used for different application areas like photodynamic therapy.

Adoptability assessment of HCDI and RCCI modes in plug-in parallel hybrid electric vehicles using sustainable fuels and model-based torque structure calibration strategies

In this research, a comparative analysis of plug-in hybrid electric vehicle (PHEV) engine was conducted with two advanced low temperature combustion (LTC) techniques viz. Homogeneous charge with direct injection (HCDI) and reactivity-controlled compression ignition (RCCI), utilizing alternative fuels diethyl ether (DEE) and ethanol. This was attempted to exhibit the promising potential of LTC based PHEV to achieve the highest efficiency and low emissions. The experimental results disclosed that DEE powered HCDI can achieve superior thermal efficiency with an impressive peak torque of 14 Nm. On the other hand, ethanol powered RCCI produced a peak torque of 17.5 Nm with remarkable emission reduction characteristics, especially CO2. It was vindicated that HCDI-PHEV outperformed the RCCI-PHEV in terms of fuel economy despite both HCDI and RCCI being lower than the conventional diesel PHEV. The NOx emissions from HCDI were almost negligible. About 20 % lower nitrogen oxide (NOx) emissions were recorded for RCCI respectively as compared to diesel (36 g/s). HCDI and RCCI showcased about 2 and 1.3 times higher soot emissions than diesel (7.12 g/s). Fuel economy was improved by 15 % and 43 % respectively under HCDI and RCCI modes over the conventional diesel mode of PHEV operation. When the PHEV was operated continuously for about 3.5 h, the battery's SOC dropped from 80 % to 45 % under diesel operation, whereas it only reached 50 % and 60 % under RCCI and HCDI mode operation. Low fuel consumption characteristics, acceptable torque production capabilities and ultralow NOx reduction potential established HCDI as the preferable choice for PHEV over RCCI.

Development of hydrothermal carbonaceous carbon/NH2-MIL-101(Fe) composite photocatalyst with in-situ production and activation of H2O2 capabilities for effective sterilization

Photocatalytic production of H2O2 has garnered significant attention, but the use of sacrificial reagents in H2O2 photosynthesis and the subsequent limited H2O2 activation efficiency would impede its practical application in water decontamination. In this work, hydrothermal carbonaceous carbon/NH2-MIL-101(Fe) (abbreviated as HTC-cf/NMIL-101(Fe)) heterojunction was fabricated through a solvothermal strategy, and serving as a self-sufficient photo-Fenton-like catalyst to in-situ generate and activate H2O2. Hydrothermal carbonaceous carbon derived from cost-effective waste coffee grounds, serving as a green and sustainable photocatalyst, demonstrated high H2O2 production rate of 612 μmol/g/h without the need for sacrificial reagents or aeration assistance. Moreover, the excellent self-sufficient photo-Fenton activity for HTC-cf/NMIL-101(Fe) can be obtained, which ascribed to the synergistic effect of self-generated H2O2 production as well as the in-situ catalytic H2O2 decomposition of •OH by Fe species in NMIL-101(Fe) and photogenerated electrons. The optimized HTC-cf/NMIL-101(Fe) photocatalyst showed simultaneous improvements in both H2O2 production and activation capabilities. Impressively, the effective circulation of Fe3+/Fe2+ in NMIL-101(Fe), aided by photogenerated electrons, results in a 5-fold increase toward H2O2 decomposition rate constant (Kd) compared to HTC-cf alone under light irradiation in open-air conditions. The enhanced charge separation and appropriate band structure of HTC-cf/NMIL-101(Fe) photocatalyst assured the high selectivity of two-electron O2-reduction to H2O2. HTC-cf/NMIL-101(Fe) displayed excellent photocatalytic sterilization efficiency against E. coli and S. aureus under visible light, and its sterilization application in actual water was also demonstrated. Notably, the adaptability of this system to ambient conditions, including open-air atmosphere, non-sacrificial reagents, and low leaching of Fe ions, highlights the promising practical utility of the HTC-cf/NMIL-101(Fe) photocatalyst.

Test results of new breeding potato hybrids in the conditions of the Volga-Vyatka region

In the period from 2016 to 2022 in the conditions of the Kirov region, an assessment of hybrid potato combinations was carried out according to the most important traits - high productivity and yield, marketable and consumer qualities of tubers. New potato hybrids bred by the Falenki Breeding Station – branch of the FARC North-East were used as the objects of the research: 6-17 (‘Gornyak’ x ‘Manifest’); 15-17 (428-05 x ‘Manifest’); 33-17 (132-07 x ‘Manifest’); 72-17 (‘Gornyak’בIrbitsky’); 78-17 (‘Udacha’ x 428-05); 120-17 (‘Utyonok’בReggi’); 25-16 (‘Kupalinka’ x 428-05). They were noted as the most promising ones according to the results of the research of 2016-2022. The medium-early ‘Nevsky’ cultivar approved for use in the Kirov region was used as a standard. According to the methodological guidelines of the potato breeding process, phenological observations, productivity assessment (on day 65), total and commercial yields were carried out in the nursery, and the chemical composition of tubers was determined. As a result, on average, over three years of testing for a complex of economically valuable traits, a promising breeding number 33-17 was identified – a significant excess of the total yield (29.9 t/ha) over the standard ‘Nevsky‘ cultivar was 14.6 t/ha. The tubers are oval with a red, slightly reticulated skin, the eyes are small, unpainted, the flesh is white. In addition, when evaluating the productive capabilities of potato hybrids, the coefficient of adaptability of breeding number 33-17 was 1.40, which makes it possible to recommend it for cultivation in this region in order to obtain stable yields.

Design and research of multi-information fusion RFID sensor for fruit and vegetable quality detection.

With the increasing demand for fruits and vegetables in the market, the development of cold chain logistics has put forward higher requirements for the quality of fruits and vegetables in storage. To ensure the freshness of fruits and vegetables during storage and transportation and avoid unnecessary loss, it is necessary to conduct real-time detection of their odor to ensure their quality. Therefore, based on nano-composite materials combined with Radio Frequency Identification (RFID) technology, this paper designs an integrated RFID sensor that can simultaneously detect temperature, carbon dioxide, and ethanol concentrations. The test results show that the sensor has a high sensitivity of 0.25dB/°C, 0.011dB/ppm, and 0.65MHz/ppm for detecting temperature, carbon dioxide, and ethanol concentration, respectively. The sensor also uses Printed Circuit Board (PCB) technology to make the sensor base, which has the advantages of low cost, easy portability, and mass production capability. The results obtained evidencethat the system meets the requirements of environmental monitoring for fruit and vegetable storage, runs stably, and has a high use value.

Metabolic-methane mitigation by combination of probiotic Escherichia coli strain Nissle 1917 and biochar in rumen fluid in vitro fermentation of dairy cow

In response to climate change, there have been various trials to reduce methane emissions from ruminant animals in the livestock industry, and one of the typical candidates is probiotics used as feed additives. We employed Escherichia coli Nissle 1917 (EcN) as a promising probiotic for the reduction of methane emissions and investigated the molecular mechanism of methane-reducing effects using CRISPR/Cas9. The anaerobic culture of EcN with biochar (BC) could significantly increase acetate and hydrogen production. Additionally, RNA-seq analysis revealed an upregulation in the expression of acetate synthesis genes, namely, pta, poxB, and ackA. Subsequently, treatment of rumen fluid with EcN significantly decreased methane and increased acetate and propionate production, as observed through the analysis of short-chain fatty acids, and these effects could accelerate with additional supplementation of BC. The observation of changes in the microbial composition of rumen fluid following treatment with EcN and BC was made through rumen metagenomic analysis and RT-qPCR. The results revealed an increase in acetogen and propionate producing bacteria abundance and a decrease in methanogen abundance. Based on these findings, the CRISPR/Cas9 system was employed to elucidate the molecular mechanism of the methane-reducing capability of EcN. Gene deletion was performed targeting the genes poxB, ackA, and pta as key factors in acetate pathway in E. coli. The knockout of poxB, ackA, and pta could lead to the elimination of the methane-reduction of EcN as well as acetate-producing abilities. Collectively, the methane reduction ability of EcN in rumen fluid is associated with its acetate production capability, and the addition of BC significantly enhances methane mitigation capability of EcN.

Gram-Scale Synthesis of Renal-Clearable Tantalum Nanodots with High Water Solubility for Computed Tomography Imaging In Vivo.

Tantalum (Ta) emerges as a promising element for advanced computed tomography (CT) imaging probes owing to its high X-ray attenuation coefficient and excellent biocompatibility. Nevertheless, the synthesis of renally clear Ta-based imaging probes through simple methods remains a significant challenge. Herein, we introduce a simple and gram-scale approach for the synthesis of renal-clearable Ta nanodots with high water solubility for CT imaging in vivo. The Ta nanodots, coordination polymers, are fabricated via coordination reactions involving Ta(OH)5, citric acid (CA), and hydrogen peroxide. The Ta nanodots exhibit an ultrasmall hydrodynamic diameter (2.8 nm), high water solubility (1.88 g/mL, 688 mg Ta/mL), superior X-ray absorption capacity, gram-scale production capability (>10 g in lab synthesis), renal-clearable ability, and good biocompatibility. The Ta nanodots possess superior CT imaging efficacy across diverse tube voltages, enabling highly sensitive gastrointestinal CT imaging, renal CT imaging, and CT angiography (CTA). Moreover, Ta nanodots maintain robust CT imaging capabilities even at high X-ray energies, and Ta nanodots-based spectral CT achieves metallic artifacts-minimized CTA. The proposed Ta nanodots present substantial potential as a potent CT imaging probe for diagnosing various diseases.

Molecular identification of lactic acid bacteria from traditional fermented foods and screening exopolysaccharide production by using food wastes.

In this study, lactic acid bacteria (LAB) isolation from fermented foods and molecular identification using magnetic bead technology were performed. And then exopolysaccharide (EPS) production possibility was tested in agar medium, and the positive ones were selected for the next step. The bacteria that could produce higher carbohydrate level were grown in MRS medium fortified with whey and pumpkin waste. In our study, 19 different LAB species were identified from fermented products collected from different places in Hatay(Türkiye) province. In molecular identification, universal primer pairs, p806R/p8FPL, and PEU7/DG74 were used for PCR amplification. After that, PCR products purified using paramagnetic bead technology were sequenced by the Sanger sequencing method. The dominant species, 23.8% of the isolates, were identified as Lactiplantibacillus plantarum. As a technological property of LAB, exopolysaccharide production capability of forty-two LAB isolate was tested in agar medium, and after eleven isolates were selected as positive. Two LAB (Latilactobacillus curvatus SHA2-3B and Loigolactobacillus coryniformis SHA6-3B) had higher EPS production capability when they were grown in MRS broth fortified with pumpkin waste and whey. The highest EPS content (1750mg/L glucose equivalent) was determined in Loigolactobacillus coryniformis SHA6-3B grown in MRS broth fortified with 10% pumpkin waste. Besides the produced EPS samples were validated with FTIR and SEM methods.

Advancements in Synthetic Biology for Enhancing Cyanobacterial Capabilities in Sustainable Plastic Production: A Green Horizon Perspective

This comprehensive review investigates the potential of cyanobacteria, particularly nitrogen-fixing strains, in addressing global challenges pertaining to plastic pollution and carbon emissions. By analyzing the distinctive characteristics of cyanobacteria, including their minimal growth requirements, high photosynthetic efficiency, and rapid growth rates, this study elucidates their crucial role in transforming carbon sequestration, biofuel generation, and biodegradable plastic production. The investigation emphasizes cyanobacteria’s efficiency in photosynthesis, positioning them as optimal candidates for cost-effective bioplastic production with minimized land usage. Furthermore, the study explores their unconventional yet promising utilization in biodiesel production, mitigating environmental concerns such as sulfur emissions and the presence of aromatic hydrocarbons. The resulting biodiesel exhibits significant combustion potential, establishing cyanobacteria as a viable option for sustainable biofuel production. Through a comprehensive assessment of both achievements and challenges encountered during the commercialization process, this review offers valuable insights into the diverse contributions of cyanobacteria. Its objective is to provide guidance to researchers, policymakers, and industries interested in harnessing bio-inspired approaches for structural and sustainable applications, thereby advancing global efforts towards environmentally conscious plastic and biofuel production.

Novel coupled hydromechanical model considering multiple flow mechanisms for simulating underground hydrogen storage in depleted low-permeability gas reservoir

Depleted low-permeability gas reservoirs are the primary storage media for underground hydrogen storage (UHS). An accurate assessment of the geomechanics and complex flow mechanisms in a depleted low-permeability gas reservoir is crucial for predicting the injection and production capabilities of UHS facilities. This paper proposes a novel hydromechanical multicomponent model that couples non-Darcy flow, relative permeability hysteresis (RPH), and geomechanics to evaluate the hydrogen storage capacity of UHS facilities in a depleted low-permeability gas reservoir. The coupled model was solved using a fully coupled strategy, employing the finite volume method to solve non-Darcy flow equations and the virtual element method to solve geomechanical problems. The results indicate that the high-velocity non-Darcy (hvnD) and geomechanical effects reduce the working gas volume by 12.82% and 10.56%, respectively. Additionally, the low-velocity non-Darcy (lvnD) effect causes the third stress/strain distribution to exhibit a “focusing” phenomenon, resulting in a 13.19% reduction in the working gas volume. This paper also describes a case where residual gas is captured by mechanisms such as “water lock” and “capillary capture” through the RPH effect. Considering the RPH effect, the gas-water transition zone expanded by 30%, and the peak volume of gas-containing pores in the water zone increased by 1.48 times. Ignoring the RPH effect led to a 19.73% overestimation of the utilization rate of the gas-containing pore space. Hence, this study achieves safe and successful seasonal hydrogen storage and provides theoretical support for designing multicycle operational plans.

GLOBAL FOOD SECURITY: SECURITY FACTORS AND CURRENT TRENDS

The issue of ensuring global food security remains one of the leading and decisive issues for all countries in the world, without exception. The implementation of the goals of sustainable development, which have become a strategic priority for society, has separated the problems of overcoming hunger and ensuring the health of the population as key and the main priority for the further existence of humanity. The article examines the essence of the category "food security" from various scientific and methodological positions and conducts a comprehensive review of the prominent trends that accompany the aspects of solving the issue of achieving food security on a global scale. The main elements of the agro-food system as a resource base for ensuring global food security are separated: 1) a sufficient amount of safe, high-quality food; 2) healthy nutrition of the population; 3) inclusive distribution of income and expenses of the population; 4) prevention of climate change, sustainability and conservation of biological resources. A set of key factors influencing the possibilities of ensuring global food security has been identified: population size, science and technology, climate change, imperfect global food distribution mechanisms, problems of gender inequality, urbanization processes, and inclusive gaps between the living conditions of the population, the growing scale of political and military conflicts The model's architecture, which contains the essential characteristics of achieving global food security, is outlined. Critical trends for achieving global food security were identified and assessed. Among them: the relative stability of the problem of world hunger, in particular, in individual world regions; increasing the level of dependency, hunger, and gender inequality; the growth of inclusive gaps in the economic availability of food; increase in the level of urbanization of the population; the health problem of a large part of the world's population and the strengthening of the trend of healthy eating. Ukraine, a significant player in the global agri-food market, holds substantial potential to combat the world's hunger crisis. The key to unlocking this potential lies in resolving the ongoing military conflict within Ukraine's borders and restoring the country's agricultural resources and production capabilities through sustainable development.

Plant‐Derived Extracellular Vesicles as Potential Emerging Tools for Cancer Therapeutics

AbstractExtracellular vesicles (EVs) are membranous structures secreted by cells that play important roles in intercellular communication and material transport. Due to its excellent biocompatibility, lipophilicity, and homing properties, EVs have been used as a new generation of drug delivery systems for the diagnosis and treatment of tumors. Despite the potential clinical benefits of animal‐derived extracellular vesicles (AEVs), their large‐scale production remains sluggish due to the exorbitant cost of cell culture, challenging quality control measures, and limited production capabilities. This constraint significantly hinders their widespread clinical application. Plant‐derived extracellular vesicles (PEVs) share similar functionalities with AEVs, yet they hold several advantages including a wide variety of source materials, cost‐effectiveness, ease of preparation, enhanced safety, more stable physicochemical properties, and notable efficacy. These merits position PEVs as promising contenders with broad potential in the biomedical sector. This review will elucidate the advantages of PEVs, delineating their therapeutic mechanisms in cancer treatment, and explore the prospective applications of engineered PEVs as targeted delivery nano‐system for drugs, microRNAs, small interfering RNAs, and beyond. The aim is to heighten researchers’ focus on PEVs and expedite the progression from fundamental research to the transformation of groundbreaking discoveries.

Poverty, Inequality, and Social Protection in Ethiopia: A Policy Brief

In this brief commentary the author argues that social protection is not a useful approach to addressing poverty and inequality in low-income countries like Ethiopia. Instead, the author suggests focusing on wealth creation and the capability development approach to poverty reduction. To achieve this, the author proposes targeted state investment expenditure to enhance the productive capabilities of the economically vulnerable population. Besides, the social protection system should prioritize the design and implementation of livelihood improving investment programs, as in-kind or cash transfer support is not sustainable given the elevated level of economic vulnerability. The effectiveness of Ethiopia’s social protection system lies on improving coverage, targeting deserving individuals, and sustainable financing solutions.

CRP and IHF act as host regulators in Royal Jelly's antibacterial activity

Royal Jelly (RJ) is a natural substance produced by honeybees, serving not only as nutrition for bee brood and queens but also as a functional food due to its health-promoting properties. Despite its well-known broad-spectrum antibacterial activity, the precise molecular mechanism underlying its antibacterial action has remained elusive. In this study, we investigated the impact of RJ on the bacteria model MG1655 at its half-maximal inhibitory concentration, employing LC–MS/MS to analyze proteomic changes. The differentially expressed proteins were found to primarily contribute to the suppression of gene expression processes, specifically transcription and translation, disrupting nutrition and energy metabolism, and inducing oxidative stress. Notably, RJ treatment led to a marked inhibition of superoxide dismutase and catalase activities, resulting in heightened oxidative damage and lipid peroxidation. Furthermore, through a protein–protein interaction network analysis using the STRING database, we identified CRP and IHF as crucial host regulators responsive to RJ. These regulators were found to play a pivotal role in suppressing essential hub genes associated with energy production and antioxidant capabilities. Our findings significantly contribute to the understanding of RJ's antibacterial mechanism, highlighting its potential as a natural alternative to conventional antibiotics. The identification of CRP and IHF as central players highlights the intricate regulatory networks involved in RJ's action, offering new targets for developing innovative antimicrobial strategies.

Cairo pentagon tessellated covalent organic frameworks with mcm topology for near-infrared phototherapy

Despite the prevalent of hexagonal, tetragonal, and triangular pore structures in two-dimensional covalent organic frameworks (2D COFs), the pentagonal pores remain conspicuously absent. We herein present the Cairo pentagonal tessellated COFs, achieved through precisely chosen geometry and metrics of the linkers, resulting in unprecedented mcm topology. In each pentagonal structure, porphyrin units create four uniform sides around 15.5 Å with 90° angles, while tetrabiphenyl unit establish a bottom edge about 11.6 Å with 120° angles, aligning precisely with the criteria of Cairo Pentagon. According to the narrow bandgap and strong near-infrared (NIR) absorbance, as-synthesized COFs exhibit the efficient singlet oxygen (1O2) generation and photothermal conversion, resulting in NIR photothermal combined photodynamic therapy to guide cancer cell apoptosis. Mechanistic studies reveal that the good 1O2 production capability upregulates intracellular lipid peroxidation, leading to glutathione depletion, low expression of glutathione peroxidase 4, and induction of ferroptosis. The implementation of pentagonal Cairo tessellations in this work provides a promising strategy for diversifying COFs with new topologies, along with multimodal NIR phototherapy.

Etika Periklanan dan Perlindungan Anak dalam Iklan Mie Sedaap Papa Hidup Lagi

In Indonesia's advertising context, upholding ethics is a crucial aspect for practitioners. The Indonesian Advertising Council recognizes the importance of ethics in creating advertisements that are not only creative but also ethical. As a strategic instrument in marketing communication, advertising must prioritize ethics when conveying messages to the public. Creating ethical ads requires specific methods to conform to established standards. This article evaluates ethical violations in the 'Papa Hidup Lagi' edition of Mie Sedaap's advertisement. This study will examine scenes that allegedly violate the advertising code of ethics in detail. This study uses a qualitative approach with content analysis techniques and data validity is strengthened with supporting related reference sources. Based on the research results, it was found that the 'Papa Hidup Lagi' edition of Mie Sedaap's advertisement violated Indonesian Advertising Ethics, especially Chapter. III A, article 3.1, sub-article 3.1.2 deals with depicting children in situations that are misleading or inappropriate to be shown. In addition, this advertisement violates the Chapter. III A, point 1.27 states that the benefits of a product must be conveyed honestly, correctly, and responsibly, and not add other benefits beyond the product's capabilities.