Event Abstract Back to Event Participation of Treg cells in equine Recurrent Airway Obstruction Claudio Henriquez1*, Gabriel Moran1, Barbara Perez1, Natalia Morales1 and Hugo Folch1 1 Universidad Austral de Chile, Immunology, Chile Recurrent Airway Obstruction (RAO) is a common allergic inflammatory disease that affects stabled adult horses. As etiological factor, environmental air borne antigens are implicated, and airway hypersensitivity types I and III, are well recognized factors of the disease. A deeper study of the disease, clearly show that other immunologic components are involved. In this work we explored the participation of the T regulatory cells (Tregs) in equine RAO. For this purpose, a RAO susceptible herd of nine horses were exposed to Aspergillus fumigatus and the clinical condition monitored before and after the allergen exposure, obtaining between other samples bronchioalveolar lavage fluid (BALF) in order to evaluate the cell content the airways, and in particular the presence of Treg cells. The results show that the Treg CD-4+, CD-25+, FoxP3+ increases in BALF obtained from healthy animals from 0.56% to 1.59% in RAO horses, if we consider as Treg population the CD-4+, FoxP3+, CD25- cells, we find that the content of this sub-population increases from 1.80% to 3.54% in normal and RAO positives animals. This increment represent represents the largest contribution to the increase of total FoxP3 cells in airways and has a direct correlation with number of neutrophils in BALF as well as with the clinical and the mucus score shown by each animal. The role of Treg in the resolution of the disease as well as the significance of the great number of FoxP3 positives Tregs that do not show the CD-25 molecule in the membrane are discussed. Acknowledgements Fondecty11100196, Fondecyt 1130355, Postgraduate School of Veterinary Medicine Department References Moran G, Folch H, Henriquez C, Ortloff A, Barria M. (2012). Reaginic antibodies from horses with recurrent airway obstruction produce mast cell stimulation. Vet Res Commun. 36, 251-8. Keywords: regulatory T cells (T-Regs), RAO, Horses, Respiratory diseases, BALF Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Henriquez C, Moran G, Perez B, Morales N and Folch H (2013). Participation of Treg cells in equine Recurrent Airway Obstruction. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00090 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Claudio Henriquez, Universidad Austral de Chile, Immunology, Valdivia, Chile, claudio.henriquez@uach.cl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Claudio Henriquez Gabriel Moran Barbara Perez Natalia Morales Hugo Folch Google Claudio Henriquez Gabriel Moran Barbara Perez Natalia Morales Hugo Folch Google Scholar Claudio Henriquez Gabriel Moran Barbara Perez Natalia Morales Hugo Folch PubMed Claudio Henriquez Gabriel Moran Barbara Perez Natalia Morales Hugo Folch Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.