IL-17A producing mast cells as therapeutic target in spondyloarthritis

N Yeremenko,P P Tak,I Gofita,J Canete,T Noordenbos,D Baeten

doi:10.1186/1479-5876-8-s1-p42

Abstract

We recently observed a remarkably increased synovial infiltration with c-kit-positive mast cells in non-psoriatic and psoriatic spondyloarthritis (SpA) versus rheumatoid arthritis (RA).

Highlights

We recently observed a remarkably increased synovial infiltration with c-kit-positive mast cells in non-psoriatic and psoriatic spondyloarthritis (SpA) versus rheumatoid arthritis (RA).role of cytokine production by mast cells in the synovial inflammation in SpA, we used imatinib mesylate to block c-kit tyrosine kinase in ex vivo synovial tissue cultures
Aim As these mast cells were not degranulated, we investigated whether they could contribute to synovial inflammation by cytokine production, with special focus on IL-17
Mast cells were identified in synovial tissue by immunostaining for c-kit or mast cell tryptase

Summary

Introduction

We recently observed a remarkably increased synovial infiltration with c-kit-positive mast cells in non-psoriatic and psoriatic spondyloarthritis (SpA) versus rheumatoid arthritis (RA). Role of cytokine production by mast cells in the synovial inflammation in SpA, we used imatinib mesylate to block c-kit tyrosine kinase in ex vivo synovial tissue cultures. C-kit blockade strongly reduced IL-6 and IL-8 and IL-17A production by SpA synovial biopsies. Aim As these mast cells were not degranulated, we investigated whether they could contribute to synovial inflammation by cytokine production, with special focus on IL-17

Patients and methods

Results

Conclusion