Producing Bacillus Sp Research Articles

Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6

A thermostable alkaline α-amylase producing Bacillus sp. ANT-6 was isolated from soil samples. Enzyme synthesis occurred at temperatures between 25 and 45 °C with an optimum of 37 °C. There was a slight variation in amylase synthesis within the pH range 7 and 11 with an optimum pH of 9. The optimum temperatures for amylase production and growth were the same. Analyses of the enzyme by sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed a single band, which show amylolytic activity, detected in starch gel. The relative molecular mass of the partial purified enzyme was estimated to be 94 500 Da. The enzyme showed optimum activity at pH 10.5 and 80 °C. The partial purified enzyme was highly active in the alkaline range of pH (9.5–13), and it was completely active up to 100 °C retaining 85.5% initial activity at pH 10.5. Enzyme activity was enhanced in the presence of 5 mM CaCl 2 (110%) and 3 mM PMSF (103%) , and inhibition with 5 mM by Zn, Na, Na-sulphide, EDTA (10 mM), Urea (8 M) and SDS (0.1%) was obtained 36.9, 21.5, 22.2, 4.90, 86% and 10.27, respectively. The enzyme was stable (55%) at high alkaline pH for 24 h. So our result showed that the enzyme was both alkaline, thermostable, thermopile and chelator resistant. The ANT-6 amylase enzyme may be suitable in liquefaction of starch, in detergent and textile industries and in other industrial applications.

抗菌物質産生性細菌ＡＢ８９について Ｉ． 産生菌の諸性質ならびに抗菌物質抽出法

抗菌物質産生性細菌ＡＢ８９について Ｉ． 産生菌の諸性質ならびに抗菌物質抽出法

The isolation of a thermophilic biosurfactant producing Bacillus SP

A thermophilic Bacillus strain has been isolated on a hydrocarbon containing medium and grew at up to 50°C. This strain produced biosurfactant and its 20h old culture broth had low surface and interfacial tension (27–29 and 1.5 mN/m, respectively). It emulsified Kerosene and other hydrocarbons efficiently (E−24 = 95 %) and was able to recover more than 95 % of the residual oil from sandpack columns. Potential uses in oil industries are discussed.