Processing Steps Research Articles

Exploring the antioxidant realm of green tea: From extraction to fortification

AbstractGreen tea, a widely consumed beverage with a long history of traditional use has garned renewed scientific interest due to its potential health benefits. Unlike other tea types, green tea undergoes minimal oxidation and thus preserving its potent catechins and antioxidant properties. This review devels into the fascinating chemistry of green tea, exploring how processing steps like fixation and rolling, shape its distinct profile. We will further explore the potential health benefits associated with green tea consumption, acknowledging the existing research focus on tea polyphenols and aiming to provide a broader perspective on its potential health impact. Finally, the review examines the emerging application of green tea extract fortification in food matrices. This innovative approach offers exciting possibilities for enhancing food stability and sensory appeal while potentially contributing to a range of health benefits.

One step production from the monomer of poly(L‐lactide)/flax biocomposites by thermoplastic resin transfer molding: Mechanical properties and aging

AbstractDevelopment of biocomposites is becoming a necessity due to environmental concerns. Polylactide is a matrix of choice, regarding its bio‐sourced and compostable nature along with its mechanical properties. In this study, poly(L‐lactide)/flax biocomposites were produced using thermoplastic resin transfer molding (TP‐RTM) in one step process from the monomer, affording sustainable and fully compostable composite materials. The polymerization of L‐lactide (L‐LA) monomer was promoted by tin octoate, affording poly(L‐lactide) (PLLA) matrices displaying monomer conversions superior to 96% and mass‐average molar masses above 140,000 g/mol. Flax fibers were used as received without any functionalization which is usually conducted to prevent the hydroxyl groups to conduct side reactions. Mechanical testing revealed bending modulus of 9.3 GPa and bending strength of 177 MPa comparable to existing literature values for PLLA/flax composites produced by other techniques. Digital microscopy analysis provided insights into impregnation quality and void characterization within the composites. In addition, the durability of these biocomposites was also evaluated via accelerated aging tests.Highlights PLLA/flax biocomposites were produced by TP‐RTM for the first time. In situ polymerization of the PLLA matrix associated with non‐functionalized flax fabrics was conducted. Bending properties were found in line with those of PLLA/flax composites produced by compression molding. Aging tests under UV at 50°C of PLLA/flax biocomposites were conducted.

Pd-Catalyzed Ring-Opening Polymerization of Cyclobutanols through C(sp3)-C(sp3) Bond Cleavage.

A new approach to ring-opening polymerization (ROP) based on C(sp3)-C(sp3) bond cleavage is reported. This process is based on the ability of Pd to promote both the β-carbon elimination of a bifunctional cyclobutanol precursor and the C-C coupling process with the resulting Pd-alkyl intermediate. Consequently, novel polyketone materials are obtained. Owing to the modular synthesis of the used cyclobutanol monomers, the present ROP reaction allows the introduction of substitution patterns in the polymeric chain that are not accessible by current polyketone synthesis methodologies. We have explored in detail the initiation, propagation, and termination steps of this new polymerization process.

Adaptive second-order backstepping control for a class of 2DoF underactuated systems with input saturation and uncertain disturbances

An adaptive second-order backstepping control algorithm is proposed for a kind of two degrees of freedom (2DoF) underactuated systems. The system dynamics is transformed into a nonlinear feedback cascade system with an improved global change of coordinates. Fully taking the cascade structure into consideration and in order to simplify the design process, each step in the backstepping process is designed for a second-order subsystem. Two neural networks are applied to approximate system unknown functions and two adaptive laws are designed to estimate the upper bound of the sum of approximation error and external disturbances. To overcome the explosion problem of complexity, a second-order filter is applied to produce the virtual control and its second-order derivative that is needed in the next backstepping step. Two auxiliary dynamic systems are proposed and integrated into the backstepping process to eliminate the effects of filtering error and input saturation. The system stability is analyzed by the Lyapunov stability theory and verified by numerical simulations with two 2DoF benchmark underactuated systems: the translational oscillator with a rotational actuator (TORA) and the inertial wheel pendulum (IWP).

Adaptive second-order backstepping control for a class of 2DoF underactuated systems with input saturation and uncertain disturbances

An adaptive second-order backstepping control algorithm is proposed for a kind of two degrees of freedom (2DoF) underactuated systems. The system dynamics is transformed into a nonlinear feedback cascade system with an improved global change of coordinates. Fully taking the cascade structure into consideration and in order to simplify the design process, each step in the backstepping process is designed for a second-order subsystem. Two neural networks are applied to approximate system unknown functions and two adaptive laws are designed to estimate the upper bound of the sum of approximation error and external disturbances. To overcome the explosion problem of complexity, a second-order filter is applied to produce the virtual control and its second-order derivative that is needed in the next backstepping step. Two auxiliary dynamic systems are proposed and integrated into the backstepping process to eliminate the effects of filtering error and input saturation. The system stability is analyzed by the Lyapunov stability theory and verified by numerical simulations with two 2DoF benchmark underactuated systems: the translational oscillator with a rotational actuator (TORA) and the inertial wheel pendulum (IWP).

Does the XPA–FEN1 Interaction Concern to Nucleotide Excision Repair or Beyond?

Nucleotide excision repair (NER) is the most universal repair pathway, which removes a wide range of DNA helix-distorting lesions caused by chemical or physical agents. The final steps of this repair process are gap-filling repair synthesis and subsequent ligation. XPA is the central NER scaffolding protein factor and can be involved in post-incision NER stages. Replication machinery is loaded after the first incision of the damaged strand that is performed by the XPF–ERCC1 nuclease forming a damaged 5′-flap processed by the XPG endonuclease. Flap endonuclease I (FEN1) is a critical component of replication machinery and is absolutely indispensable for the maturation of newly synthesized strands. FEN1 also contributes to the long-patch pathway of base excision repair. Here, we use a set of DNA substrates containing a fluorescently labeled 5′-flap and different size gap to analyze possible repair factor–replication factor interactions. Ternary XPA–FEN1–DNA complexes with each tested DNA are detected. Furthermore, we demonstrate XPA–FEN1 complex formation in the absence of DNA due to protein–protein interaction. Functional assays reveal that XPA moderately inhibits FEN1 catalytic activity. Using fluorescently labeled XPA, formation of ternary RPA–XPA–FEN1 complex, where XPA accommodates FEN1 and RPA contacts simultaneously, can be proposed. We discuss possible functional roles of the XPA–FEN1 interaction in NER related DNA resynthesis and/or other DNA metabolic processes where XPA can be involved in the complex with FEN1.

The Thermal Energy Storage Characteristics of Oleic Acid Modified ZnO‐Decorated Polymer Matrix‐Supported Composite Phase Change Materials: Synthesis and Characterization

AbstractIn this study, modified nano zinc oxide (ZnO)‐reinforced polymer‐supported novel thermally enhanced form‐stable composite phase change materials (PCMs) are presented, which are prepared via water in oil emulsion polymerization and following impregnation process steps. First, ZnO nanoparticles are modified with oleic acid (OA) to obtain lipophilic structures for emulsion stability, which are designed to take a role as a heat transfer activator. To ensure the shape stabilization of n‐hexadecane used as organic PCM, polymeric support materials are synthesized in the presence of modified ZnO nanoparticles (ZnO@OA). The polymeric frameworks exhibit open porous morphology, and the thermal stability of the support matrix improves with the addition of ZnO nanofiller. In the second step, composite PCMs are prepared by incorporation of n‐hexadecane with the solvent‐assisted vacuum impregnation method into polymer composites. The 1.0% ZnO@OA incorporated composite PCM has the highest incorporation ratio and exhibits a thermal storage capability (η) of 100%. According to the T‐history and thermal conductivity tests, it is observed that the heat conduction rate is enhanced with the addition of ZnO@OA nanofiller. The conclusion is that the obtained ZnO@OA integrated composite PCMs have a remarkable potential for latent heat storage applications requiring low temperature in the range of 5–25 °C.

Influence of the 3-Hydroxyvalerate Content on the Processability, Nucleating and Blending Ability of Poly(3-Hydroxybutyrate-co-3-hydroxyvalerate)-Based Materials.

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate (P(3HB-co-3HV) copolymers are an attractive class of biopolymers whose properties can be tailored by changing the 3-hydroxyvalerate monomer (3HV) concentration, offering the possibility of counteracting problems related to high crystallinity, brittleness, and processability. However, there are few studies about the effects of 3HV content on the processability of copolymers. The present study aims to provide new insights into the effect of 3HV content on the processing step including common practices like compounding, addition of nucleation agents and/or amorphous polymers as plasticizers. P(3HB-co-3HV)-based films containing 3, 18, and 28 mol % 3HV were processed into films by extrusion and subsequent molding. The characterization results confirmed that increasing the 3HV content from 3 to 28 mol % resulted in a decrease in the melting point (from 175 to 100 °C) and an improvement in mechanical properties (i.e., elongation at break from 7 ± 1% to 120 ± 3%). The behavior of P(3HB-co-3HV) in the presence of additives was also investigated. It was shown that an increase in the 3HV content leads to better miscibility with amorphous polymers.

The Thermal Energy Storage Characteristics of Oleic Acid Modified ZnO‐Decorated Polymer Matrix‐Supported Composite Phase Change Materials: Synthesis and Characterization

AbstractIn this study, modified nano zinc oxide (ZnO)‐reinforced polymer‐supported novel thermally enhanced form‐stable composite phase change materials (PCMs) are presented, which are prepared via water in oil emulsion polymerization and following impregnation process steps. First, ZnO nanoparticles are modified with oleic acid (OA) to obtain lipophilic structures for emulsion stability, which are designed to take a role as a heat transfer activator. To ensure the shape stabilization of n‐hexadecane used as organic PCM, polymeric support materials are synthesized in the presence of modified ZnO nanoparticles (ZnO@OA). The polymeric frameworks exhibit open porous morphology, and the thermal stability of the support matrix improves with the addition of ZnO nanofiller. In the second step, composite PCMs are prepared by incorporation of n‐hexadecane with the solvent‐assisted vacuum impregnation method into polymer composites. The 1.0% ZnO@OA incorporated composite PCM has the highest incorporation ratio and exhibits a thermal storage capability (η) of 100%. According to the T‐history and thermal conductivity tests, it is observed that the heat conduction rate is enhanced with the addition of ZnO@OA nanofiller. The conclusion is that the obtained ZnO@OA integrated composite PCMs have a remarkable potential for latent heat storage applications requiring low temperature in the range of 5–25 °C.

The cytidine deaminase APOBEC3A regulates nucleolar function to promote cell growth and ribosome biogenesis.

Cancer initiates as a consequence of genomic mutations and its subsequent progression relies in part on increased production of ribosomes to maintain high levels of protein synthesis for unchecked cell growth. Recently, cytidine deaminases have been uncovered as sources of mutagenesis in cancer. In an attempt to form a connection between these 2 cancer driving processes, we interrogated the cytidine deaminase family of proteins for potential roles in human ribosome biogenesis. We identified and validated APOBEC3A and APOBEC4 as novel ribosome biogenesis factors through our laboratory's established screening platform for the discovery of regulators of nucleolar function in MCF10A cells. Through siRNA depletion experiments, we highlight APOBEC3A's requirement in making ribosomes and specific role within the processing and maturation steps that form the large subunit 5.8S and 28S ribosomal (r)RNAs. We demonstrate that a subset of APOBEC3A resides within the nucleolus and associates with critical ribosome biogenesis factors. Mechanistic insight was revealed by transient overexpression of both wild-type and a catalytically dead mutated APOBEC3A, which both increase cell growth and protein synthesis. Through an innovative nuclear RNA sequencing methodology, we identify only modest predicted APOBEC3A C-to-U target sites on the pre-rRNA and pre-mRNAs. Our work reveals a potential direct role for APOBEC3A in ribosome biogenesis likely independent of its editing function. More broadly, we found an additional function of APOBEC3A in cancer pathology through its function in ribosome biogenesis, expanding its relevance as a target for cancer therapeutics.

Digital light processing printed hydrogel scaffolds with adjustable modulus

Hydrogels are extensively explored as biomaterials for tissue scaffolds, and their controlled fabrication has been the subject of wide investigation. However, the tedious mechanical property adjusting process through formula control hindered their application for diverse tissue scaffolds. To overcome this limitation, we proposed a two-step process to realize simple adjustment of mechanical modulus over a broad range, by combining digital light processing (DLP) and post-processing steps. UV-curable hydrogels (polyacrylamide-alginate) are 3D printed via DLP, with the ability to create complex 3D patterns. Subsequent post-processing with Fe3+ ions bath induces secondary crosslinking of hydrogel scaffolds, tuning the modulus as required through soaking in solutions with different Fe3+ concentrations. This innovative two-step process offers high-precision (10 μm) and broad modulus adjusting capability (15.8–345 kPa), covering a broad range of tissues in the human body. As a practical demonstration, hydrogel scaffolds with tissue-mimicking patterns were printed for cultivating cardiac tissue and vascular scaffolds, which can effectively support tissue growth and induce tissue morphologies.

Anodization-free fabrication process for high-quality cross-type Josephson tunnel junctions based on a Nb/Al-AlO x /Nb trilayer

Josephson tunnel junctions form the basis for various superconductor electronic devices. For this reason, enormous efforts are routinely taken to establish and later on maintain a scalable and reproducible wafer-scale manufacturing process for high-quality Josephson junctions. Here, we present an anodization-free fabrication process for Nb/Al-AlO x /Nb cross-type Josephson junctions that requires only a small number of process steps and that is in general intrinsically compatible with wafer-scale fabrication. We show that the fabricated junctions are of very high quality and, compared to other junction types, exhibit not only a significantly reduced capacitance but also an almost rectangular critical current density profile. Our process hence enables the usage of low capacitance Josephson junctions for superconductor electronic devices such as ultra-low noise dc-superconducting quantum interference devices (SQUIDs), microwave SQUID multiplexers based on non-hysteretic rf-SQUIDs and RFSQ circuits.

TUMDOT–MUC: Data Collection and Processing of Multimodal Trajectories Collected by Aerial Drones

Currently available trajectory data sets undoubtedly provide valuable insights into traffic events, the behavior of road users and traffic flow theory, thus enabling a wide range of applications. However, there are still shortcomings that need to be addressed: (i) the continuous temporal recording (ii) of a coherent area covering several intersections (iii) with the detection of all road users, including pedestrians and cyclists. Therefore, this study focuses on the design of a large-scale aerial drone observation in the city of Munich, Germany, as well as the processing steps and the description of the resulting data set. Using twelve camera-equipped, unmanned aerial drones, the observation monitored an inner urban road section with a length of 700 m continuously for several hours during the afternoon peak hours on two working days. The trajectories of all road users were then extracted from the videos and post-processed in order to obtain a coherent and accurate data set. The resulting trajectories contain information on the category, dimensions, location, velocity, acceleration and orientation of each road user at each frame, merged continuously in time and space across several drone observation areas and subsequent time slots. The data, therefore, includes various interactions between different modes of motorized traffic and active mobility users like pedestrians and cyclists. The whole data set and the supporting data are available open source for research purposes to ensure global accessibility.

Logistics 4.0 – digital transformation with smart connected tracking and tracing devices

Tracking and tracing devices can provide real-time information from the supply chain and enable digital transformation in the logistics and supply chain industry. In this connection, Logistics 4.0 refers to the potential for information technology and smart connected assets to be used in logistics in the same way as the Industry 4.0 concept which has been applied in operations and manufacturing. This paper analyses current tracking and tracing-focused applications that can provide value for logistics operations through a case study approach.This study employs an exploratory multiple-case study approach, which is based on interviews with development project stakeholders. The paper analyses three industrial case studies and how tracking/tracing applications are connected to value processes.The findings of this study show that the value of Logistics 4.0, through the deployment of tracking/tracing applications, is delivered in terms of operational efficiency, visibility, transparency, and safety/security. The payback depends on the volume of transactions, the possibility of reusing the tracking tags, the duration of trips, and the supply chain structure. The paper provides insight into how Logistics 4.0 technology can enhance logistics performance value. Based on the analysis, the study proposes the following potential application domains: (1) intermodal tracking of the shipments for operations control, (2) asset management of containers, and (3) certification of the process steps and authenticity.

Generation of precision microstructures based on reconfigurable photoresponsive hydrogels for high-resolution polymer replication and microoptics

Microstructured molds are essential for fabricating various components ranging from precision optics and microstructured surfaces to microfluidics. However, conventional fabrication technology such as photolithography requires expensive equipment and a large number of processing steps. Here, we report a facile method to fabricate micromolds based on a reusable photoresponsive hydrogel: Uniform micropatterns are engraved into the hydrogel surface using photo masks under UV irradiation within a few minutes. Patterns are replicated using polydimethylsiloxane with minimum feature size of 40 μm and smoothness of Rq ~ 3.4 nm. After replication, the patterns can be fully erased by light thus allowing for reuse as a new mold without notable loss in performance. Utilizing greyscale lithography, patterns with different height levels can be produced within the same exposure step. We demonstrate the versatility of this method by fabricating diffractive optical elements devices and a microlens array and microfluidic device with 100 µm wide channels.

Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein.

Plants are a newly developing eukaryotic expression system being explored to produce therapeutic proteins. Purification of recombinant proteins from plants is one of the most critical steps in the production process. Typically, proteins were purified from total soluble proteins (TSP), and the presence of miscellaneous intracellular proteins and cytochromes poses challenges for subsequent protein purification steps. Moreover, most therapeutic proteins like antigens and antibodies are secreted to obtain proper glycosylation, and the presence of incompletely modified proteins leads to inconsistent antigen or antibody structures. This work introduces a more effective method to obtain highly purified recombinant proteins from the plant apoplastic space. The recombinant Green fluorescent protein (GFP) is engineered to be secreted into the apoplast of Nicotiana benthamiana and is then extracted using an infiltration-centrifugation method. The GFP-His from the extracted apoplast is then purified by nickel affinity chromatography. In contrast to the traditional methods from TSP, purification from the apoplast produces highly purified recombinant proteins. This represents an important technological improvement for plant production systems.

Tested mirror precision alignment system using a pinhole point diffraction interferometer based on machine vision

In the pinhole point diffraction interferometer (PPDI), proper alignment between the reflection spot of the tested component and the pinhole is critical to obtain accurate interferograms. At present, adjusting for tilt error requires manual manipulation, and defocus error cannot be corrected. These limitations impede the instrumentation process of PPDI. To address this issue, the proposed tested mirror alignment system utilizes diffraction theory to analyze the mathematical error caused by the misalignment of the tested mirror’s reflected beam and pinhole. An alignment system based on machine vision was designed according to specific requirements. This system incorporates a CCD camera with a zoom lens, the classic PPDI with a pinhole substrate containing a lithography-made mark, and a 3-DOF stepper motor adjusting frame to mount the tested mirror. Additionally, image processing algorithms and step motor driving programs were applied to achieve precise alignment. The system implementation and experimental results indicated that the tilt errors are well-controlled, achieving the defocus error modification, making the interferogram acquisition process more convenient. From the results, this system offers desirable precision and efficiency for PPDI’s tested mirror alignment.

Automated pipeline processing X-ray diffraction data from dynamic compression experiments on the Extreme Conditions Beamline of PETRAIII.

Presented and discussed here is the implementation of a software solution that provides prompt X-ray diffraction data analysis during fast dynamic compression experiments conducted within the dynamic diamond anvil cell technique. It includes efficient data collection, streaming of data and metadata to a high-performance cluster (HPC), fast azimuthal data integration on the cluster, and tools for controlling the data processing steps and visualizing the data using the DIOPTAS software package. This data processing pipeline is invaluable for a great number of studies. The potential of the pipeline is illustrated with two examples of data collected on ammonia-water mixtures and multiphase mineral assemblies under high pressure. The pipeline is designed to be generic in nature and could be readily adapted to provide rapid feedback for many other X-ray diffraction techniques, e.g. large-volume press studies, in situ stress/strain studies, phase transformation studies, chemical reactions studied with high-resolution diffraction etc.

Discovery of Heparin Mimetic, Potent, and Selective Inhibitors of Human Clotting Factor XIIIa.

Factor XIIIa (FXIIIa) is a cysteine transglutaminase that catalyzes the last step in the coagulation process. An anion-binding site inhibition of FXIIIa is a paradigm-shifting strategy that may offer key advantages of controlled inhibition. Such an approach is likely to lead to novel FXIIIa inhibitors that do not carry bleeding risks. We previously reported a flavonoid trimer-based allosteric inhibitor of FXIIIa with moderate potency and selectivity. To further advance this approach, we evaluated a series of 27 variably sulfonated heparin mimetics against human FXIIIa. Only 13 molecules exhibited inhibitory activity at the highest concentration tested with IC50 values of 2-286 μM. Specifically, inhibitor 16 demonstrated an IC50 value of 2.4 ± 0.5 μM in a bisubstrate, fluorescence-based trans-glutamination assay. It also demonstrated a significant selectivity over other clotting factors including thrombin, factor Xa, and factor XIa as well as other cysteine enzymes including papain and tissue transglutaminase 2. Inhibitor 16 did not affect the viability of three human cell lines at a concentration that is 5-fold its FXIIIa-IC50. The molecule had a very weak effect on the activated partial thromboplastin time of human plasma at a concentration of >700 μM, further supporting its functional selectivity. Importantly, molecule 16 inhibited FXIIIa-mediated polymerization of fibrin(ogen) in a concentration-dependent manner as shown by the gel electrophoresis experiment. Michaelis-Menten kinetics revealed that the molecule competes with the Gln-donor protein substrate, i.e., dimethylcasein, but not with the Lys-donor small substrate, i.e., dansylcadaverine. Molecular modeling studies revealed that this type of molecule likely binds to an anion-binding site comprising the basic amino acids of Lys54, Lys61, Lys73, Lys156, and Arg244 among others. Overall, our work puts forward a new anion-binding site, selective, nontoxic, sulfonated heparin mimetic FXIIIa inhibitor 16 for further development as an effective and safer anticoagulant.

The Role of Ubiquitination in Osteosarcoma Development and Therapies.

The ubiquitin-proteasome system (UPS) maintains intracellular protein homeostasis and cellular function by regulating various biological processes. Ubiquitination, a common post-translational modification, plays a crucial role in the regulation of protein degradation, signal transduction, and other physiological and pathological processes, and is involved in the pathogenesis of various cancers, including osteosarcoma. Osteosarcoma, the most common primary malignant bone tumor, is characterized by high metastatic potential and poor prognosis. It is a refractory bone disease, and the main treatment modalities are surgery combined with chemotherapy. Increasing evidence suggests a close association between UPS abnormalities and the progression of osteosarcoma. Due to the complexity and pleiotropy of the ubiquitination system, each step in the ubiquitination process can be targeted by drugs. In recent years, research and development of inhibitors targeting the ubiquitin system have increased gradually, showing great potential for clinical application. This article reviews the role of the ubiquitination system in the development and treatment of osteosarcoma, as well as research progress, with the hope of improving the therapeutic effects and prognosis of osteosarcoma patients by targeting effective molecules in the ubiquitination system.