Abstract: Functional near infrared spectroscopy (fNIRS) has received increasing attention as a non-invasive, portable brain hemodynamic monitoring tool due to its potential for use in natural Settings. Traditional fNIRS systems commonly use light-emitting diodes (LEDs) as light sources, which have become the mainstream choice because of their low cost, easy integration, low heat output, and availability of a variety of near-infrared wavelengths. However, LEDs are limited by their low optical output power and broad wavelength range, which restrict their effectiveness in deep tissue penetration and signal quality. In contrast, Laser Diodes (LD) have the advantages of good monochromaticity and high optical output power, which can provide deeper tissue penetration and higher signal stability. Therefore, this study explores the feasibility of using lasers instead of leds in fNIRS systems and their impact on signal quality and imaging depth. By comparing the performance of LED and laser-based fNIRS systems, it is evident that lasers provide significant improvements in signal quality and imaging depth, although they face challenges in terms of cost, thermal management, and safety. The research in this paper provides an important reference for the future design of fNIRS system, especially in the application scenarios requiring high precision imaging.