Nitrogen Transformation Processes Research Articles

Nitrogen loss from the coastal shelf of the East China Sea: Implications of the organic matter

Organic matter is a critical factor which regulates nitrogen loss pathways of denitrification and anammox for microbes in marine ecosystems. However, only a little attention has been paid to contrasting studies on denitrification and anammox in sandy and muddy sediments, especially in the coastal continental shelf dominated by sandy sediments. This study determined the bulk properties and associated microbial nitrogen transformation processes of surface sediments in the East China Sea coastal shelf, with the aim of gaining insight into the interaction of nitrogen loss with organic matter at the molecular level. The results illustrate that nitrogen loss dominates in organic-rich muddy sediments, and its denitrification rate (14.39 nmol N g−1 h−1) and anammox rate (2.73 nmol N g−1 h−1) are greater than those of sandy sediments (denitrification rate = 5.55 nmol N g−1 h−1, anammox rate = 1.57 nmol N g−1 h−1). Furthermore, determination of the mean summed ladderanes shows higher anammox generated in the muddy sediments with a value of 167.78 ng g−1dw. Quantitative analysis demonstrated that organic-rich muddy sediments enhanced the copy number of the denitrifying functional gene nosZ and anammox functional gene hzsB. We inferred that the greater rate of nitrogen loss in muddy sediments was due to the coupling relationship between anammox and denitrification. Overall, the community distribution and abundance of denitrifying bacteria and anammox bacteria changed intricately under the influence of organic matter. Moreover, this study further improves the understanding of nitrogen loss pathways and mechanistic factors from sediments.

The photocatalytic generation of ammonia contained reusable water from antibiotics wastewater by BiOBr nanostructures with oxygen vacancies

An alternative approach for the photocatalytic generation of ammonia was proposed using N-containing antibiotics as nitrogen sources and BiOBr as photocatalyst. • A novel photocatalytic generation route of ammonia was proposed. • BiOBr nanostructures with oxygen vacancies via a solvent controlled method was used as photocatalysts. • N-containing antibiotics was used as nitrogen sources. • Ammonia can be generated while photocatalytic degradation of tetracycline. • The nitrogen transformation and ammonia generation process were explored. N-containing antibiotics wastes with high N content can cause serious environmental pollution. In order to solve the above problem, we prepared the bismuth oxybromide (BiOBr) nanostructures with oxygen vacancies and used as an efficient photocatalysts for the photocatalytic degradation of tetracycline (TC) wastewater to generate ammonia contained reusable water. Results indicated that the BiOBr nanostructures with oxygen vacancies prepared in ethanol solvent showed much enhanced photocatalytic activities, which can degrade TC completely in 1 h and produce 36.38 mg·L −1 ·g −1 of ammonia eventually. The ammonia conversion rate can reach to 48.53% at the end of the reaction. The obtained ammonia contained water without TC can be reused in agriculture field. The possible mechanism of N element transformation was proposed. This study provides an alternative approach for the photocatalytic generation of ammonia using N-containing antibiotics as nitrogen sources and opens a new perspective for the utilizing and reusing of environmental pollutants.

Metagenomics analysis unraveled the influence of sulfate radical-mediated compost nitrogen transformation process.

The mechanism of nitrogen transformation of sulfate radical (SO- 4⋅) in the process of composting is unclear. The objectives of this study were to investigate the influence of SO- 4⋅ on nitrogen biotransformation during composting and to compare the differences in physicochemical parameters and metagenomics analysis between CK (fresh dairy manure and bagasse pith) and PS (the composting raw materials added with potassium persulfate). The results indicated that SO-4⋅ guides electron transfer in the conversion of NH+4-N to NO- 3-N and breaches the extracellular polysaccharide (EPS) structure to promote nitrogen removal. Aminomonooxygenase (AMO) and nitrate reductase (NR) levels displayed an interactive relationship between microorganisms and substrates. Metagenomics analysis revealed distinct microbial community compositions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways between nitrification and denitrification. Correlation analysis indicated that Methanobrevibacter, Bacillus and Pseudomonas were closely related to these processes. This work demonstrates the effect of SO- 4⋅ on nitrogen cycling and retention, and possible mechanisms of nitrification and denitrification during composting.

Interaction mechanism between nitrogen conversion and the microbial community in the hydrodynamic heterogeneous interaction zone.

To study the inorganic nitrogen in the process of interaction of river and groundwater and the changes in the microbial community, a vertical simulation device was used to simulate groundwater recharge to river water (upwelling) and river water recharge to groundwater (downwelling). The inorganic nitrogen concentrations in the soil and water solution as well as the characteristics of the microbial community were assessed to determine the inorganic nitrogen transformation and microbial community response in the heterogeneous interaction zone under hydrodynamic action, and the interaction mechanism between nitrogen transformation and the microbial community in the interaction zone was revealed. The removal rates of NO3--N in the simulated solution reached 99.1% and 99.3% under the two fluid-groundwater conversion modes, and the prolonged hydraulic retention time (HRT) of the oxidization-reduction layer in the fine clay area and the high organic matter content made the inorganic nitrogen transformation process dominated by microorganisms more complete. The denitrification during upwelling, dominated by denitrifying bacteria in Sphingomonas, Pseudomonas, Bacillus, and Arthrobacter, was stronger than that during downwelling. Dissimilatory nitrate reduction to ammonium (DNRA), controlled by some aerobic bacteria in Pseudomonas, Bacillus, and Desulfovibrio, was more intense in downflow mode than upflow mode.

Sources and transformations of nitrate in Qixiangcuo Lake and its inflow rivers in the northern Tibetan Plateau.

Human activities and climate change input more reactive nitrogen into alpine lakes. Alpine saline lakes are usually located in endorheic watersheds at high-altitude areas, with no other drainage methods than evaporation, and are prone to accumulate nutrients. Meanwhile, alpine saline lakes are usually oligotrophic and sensitive to reactive nitrogen inputs, and even modest reactive nitrogen inputs may have significant effects on them, such as eutrophication. Nitrate is the main form of reactive nitrogen in lakes; therefore, clarifying the sources and transformations of nitrate in alpine saline lakes is important to prevent or mitigate eutrophication in alpine saline lakes. In this study, the sources and transformations of nitrate in Qixiangcuo Lake and its inflow rivers in the northern Tibetan Plateau were identified using dual nitrate isotopes and hydrochemistry. The results show that (1) the ranges of NO3- concentrations, δ15N - NO3-, and δ18O - NO3- values were 3.6 ~ 26.1μg/L, - 10.5 to + 6.0‰, and - 10.4 to + 9.2‰ in Qixiangcuo Lake and 194.4 ~ 728.1μg/L, + 5.8 ~ + 8.8‰, and - 1.9 to + 2.4‰ in its inflow rivers, respectively. The NO3- concentrations and δ15N - NO3- values were significantly lower in Qixiangcuo Lake than in its inflow rivers (P < 0.05). (2) The main sources of nitrate in both surface water and bottom water of Qixiangcuo Lake were ammonium in atmospheric deposition (mean probability estimate (MPE) 41.0% and 32.2%, respectively) and livestock manure (MPE 28.9% and 21.7%, respectively). The main sources of nitrate in the inflow rivers of Qixiangcuo Lake were domestic sewage (MPE 35.7%) and livestock manure (MPE 29.6%). (3) The main nitrogen transformation process in Qixiangcuo Lake was nitrification. The conservative mixing of multiple sources controlled the nitrate concentration and isotopic composition of Qixiangcuo Lake. Improvement in grazing area planning and the installation of sewage treatment facilities are effective measures to prevent eutrophication in Qixiangcuo Lake and its inflow rivers.

Confluences characteristics determine the influence scope of microbial community from confluence hydrodynamic zone on river network

River confluences are crucial parts of the river networks, which connect different rivers and promote material exchange between them. Joining of two rivers would cause sharp shifts of flow dynamics and form confluence hydrodynamic zone (CHZ) with specific flow structure and biogeochemical processes. However, CHZs are small areas compared with river networks, whether and how the biogeochemical processes in CHZs would influence the lower reaches and even the whole river networks remain unclear. To address this gap, the present study focused on the nitrogen dynamics in a river network consisting a series of confluences, and combined molecular biological tools, gene-centric modelling approach and SourceTracker analysis to reveal the microorganisms induced nitrogen transformation processes in CHZs and other normal hydrodynamic zones (NHZs). Results illustrated that the enriched microbial species in CHZs were associated with nitrogen transformation, such as Terrimonas and Sphingobacterium, which were reported to be vital participants in nitrate and nitrite reduction processes. The reactions rates of different nitrogen transformation processes, calculated based on functional genes abundances, further revealed that N2 production rates were significantly higher in CHZs than NHZs (p < 0.05), demonstrating CHZs were the hotspots for nitrogen removal in the researched river network. Besides, the specific microbial communities and stimulated nitrogen removal processes in CHZ would sustain in lower reaches. SourceTracker analysis illustrated that microbial communities would exert significant influences on lower reaches, and the influence scope was controlled by confluences characteristics, such as flow ratio between tributary and main stream. Higher discharge ratio would increase the influence scope of microbial community in CHZ. These results indicate that reasonable design of confluence characteristics can be applied as a critical engineering measure for the improvement of ecological health of river networks.

Nutrient status of integrated rice-crayfish system impacts the microbial nitrogen-transformation processes in paddy fields and rice yields.

Increasing rice yield is essential for alleviating global food crisis. High soil nutrient level guarantees high rice yields in conventional rice monoculture (RM) systems, but excessive unconsumed nutrients act as pollutants and can even threaten rice growth. The integrated rice-crayfish (IRC) system aims to transfer the excess nutrients from crayfish to paddy fields to improve the comprehensive utilization rate of nutrients and create additional profits, while the responding characteristics of IRC microbial communities in paddy fields and rice yields to the nutrient status remain unclear. Considering the crucial roles of microbiomes in promoting nutrient cycling for crop absorption in rice production progresses, the composition and functional characteristics of soil microbial communities from six IRC farms with variant nutrient statuses in the Yangtze River Delta were surveyed in this study. Compared with RM systems, IRC systems with appropriately improved (p < 0.05) soil quality created favorable nutrient (FN) status accompanied by 15% rice yields increase, while IRC systems with extremely high nutrients (HN) status (p < 0.01) accompanied by 14% rice yields reduction. Soil microbial diversity and network complexity were maintained in FN-IRC systems, but declined in HN-IRC systems, with the Shannon index significantly decreased by 9.2% and network density decreased from 0.135 (in RM) to 0.062. In the FN-IRC systems, the keystone taxa identified by co-occurrence networks displayed inextricably positive correlations with soil nitrification potential (calculated by normalization of amoA gene abundance) and rice yields. While in HN-IRC systems, the large loss of keystone taxa might limit soil nitrogen fixation potential (calculated by normalization of nifH gene abundance), and further rice yields. Our study indicates that soil nutrient management in IRC systems claim attention, and the improvement of nitrogen metabolism is the key to realize agricultural cleaner production.

Feammox in alluvial-lacustrine aquifer system: Nitrogen/iron isotopic and biogeochemical evidences

Groundwater nitrogen contamination is becoming increasingly serious worldwide, and natural nitrogen attenuation processes such as anaerobic ammonium oxidation coupled to iron reduction (“Feammox”) play an important role in mitigating contamination. Although there has been intensive study of Feammox in soils and sediments, still lacks research on this process in groundwater. This study makes effort to demonstrate the occurrence of Feammox in groundwater by combining information from Fe/N isotope composition, the quantitative polymerase chain reaction (qPCR) assay, and 16S rRNA gene sequencing. Poyang Lake Plain of Yangtze River in central China was selected as the case study area. The critical evidences that indicate Feammox in groundwater include favorable hydrogeochemical conditions of the alluvia-lacustrine aquifer systems, the simultaneous enrichment of 15N in ammonium and 56Fe, the relative high abundance of Acidimicrobiaceae bacterium A6, and the joint elevation of the abundance of the Feammox bacteria and the concentration of Fe(III). Redundancy analysis (RDA) indicated that Geothrix and Rhodobacter may participate directly or cooperatively in the Feammox process. Ammonium-oxidizing archaea (AOA) involved in ammonium-oxidizing or Feammox process may be stimulated by Fe(III) under a low oxygen concentration and weakly acidic condition. Anammox may be indirectly enhanced by products of the nitrogen transformation processes involving Feammox bacteria and AOA. Fe(III) concentration is an important environmental factor affecting the abundance of functional microorganisms related to nitrogen cycling and the composition of ammonium-oxidizing and iron-reducing microbes. Specific geological background (such as the widespread red soils) and anthropogenic input of ammonium, iron, and acidic substances may jointly promote Feammox in groundwater.

Soil Nitrogen Transformation Process Influenced by Litterfall Manipulation in Two Subtropical Forest Types.

Nitrogen (N) is often recognized as the primary limiting nutrient element for the growth and production of forests worldwide. Litterfall represents a significant pathway for returning nutrients from aboveground parts of trees to the soils and plays an essential role in N availability in different forest ecosystems. This study explores the N transformation processes under litterfall manipulation treatments in a Masson pine pure forest (MPPF), and Masson pine and Camphor tree mixed forest (MCMF) stands in subtropical southern China. The litterfall manipulation included litterfall addition (LA), litterfall removal (LR), and litterfall control (LC) treatments. The project aimed to examine how litterfall inputs affect the soil N process in different forest types in the study region. Results showed that soil ammonium N (NH4+-N) and nitrate N (NO3−-N) content increased under LA treatment and decreased under LR treatment compared to LC treatment. LA treatment significantly increased soil total inorganic N (TIN) content by 41.86 and 22.19% in MPPF and MCMF, respectively. In contrast, LR treatment decreased the TIN content by 10 and 24% in MPPF and MCMF compared to LC treatment. Overall, the soil net ammonification, nitrification, and N mineralization rates were higher in MCMF than in MPPF; however, values in both forests were not significantly different. LA treatment significantly increased annual net ammonification, nitrification, and mineralization in both forest types (p < 0.05) compared to LC treatment. LR treatment significantly decreased the values (p < 0.05), except for ammonification, where LR treatment did not differ substantially compared to LC treatment. Our results suggested that changes in litterfall inputs would significantly alter soil N dynamics in studied forests of sub-tropical region. Moreover, mixed forest stands have higher nutrient returns due to mixed litter and higher decomposition compared to pure forest stands.

Depth induced assembly discrepancy of multitrophic microbial communities affect microbial nitrogen transformation processes in river cross-sections

Understanding how the structures and functions of bacterial and microeukaryotic communities vary within cross-sections will improve managements aimed at restoring river ecological functions. However, no comprehensive investigation has examined how microbial community characteristics vary within cross-sections, which makes the accurate calculation and prediction of microbial metabolic processing of substances in rivers difficult. Here, the distributions, co-occurrence networks, and assemblies of bacterial and microeukaryotic communities and their feedback to nitrogen transformation in cross-sections of the Yangtze River were studied by coupling ecological theory, biogeochemistry, and DNA meta-barcoding methods. The study found that depth in cross-sections was the primary driving factor regulating the composition of sediment bacterial and microeukaryotic communities. Co-occurrence network analysis indicated that the effect of bacteria on the co-occurrence network decreased and the network become more simplified and instability with depth in river cross-sections. Quantified using the β-nearest taxon index, the H2 layer sediment (depth 10–20 m) displayed the largest variation in selection processes for microbial assemblies, while homogeneous selection and homogenizing dispersal contributed most to the bacterial and microeukaryotic assemblies in the H3 layer (depth >20 m). Cross-sectional depth and denitrification genes had a significant quadratic correlation, with the highest microbial nitrogen-removal potential occurring in the H2 layer sediment. Structural equation models showed that the sediment nitrogen distributions were regulated by distinct environmental pathways at different depths, and that the H2 layer sediment was primary driven by bacterial community. In this layer, river cross-sectional depth influenced nitrogen transformation by regulating the distribution of sediment particle sizes, which then influenced the assembly of the multitrophic microbial communities. This study will improve river management by clarifying the importance of cross-sectional depth to the ecological function of rivers.

Sources of ammonium enriched in groundwater in the central Yangtze River Basin: Anthropogenic or geogenic?

The occurrence of excessive ammonium in groundwater threatens human and aquatic ecosystem health across many places worldwide. As the fate of ammonium in groundwater systems is often affected by a complex mixture of transport and biogeochemical transformation processes, identifying the sources of groundwater ammonium is an important prerequisite for planning effective mitigation strategies. Elevated ammonium was found in both a shallow and an underlying deep groundwater system in an alluvial aquifer system beneath an agricultural area in the central Yangtze River Basin, China. In this study we develop and apply a novel, indirect approach, which couples the random forest classification (RFC) of machine learning method and fluorescence excitation-emission matrices with parallel factor analysis (EEM-PARAFAC), to distinguish multiple sources of ammonium in a multi-layer aquifer. EEM-PARAFAC was applied to provide insights into potential ammonium sources as well as the carbon and nitrogen cycling processes affecting ammonium fate. Specifically, RFC was used to unravel the different key factors controlling the high levels of ammonium prevailing in the shallow and deep aquifer sections, respectively. Our results reveal that high concentrations of ammonium in the shallow groundwater system primarily originate from anthropogenic sources, before being modulated by intensive microbially mediated nitrogen transformation processes such as nitrification, denitrification and dissimilatory nitrate reduction to ammonium (DNRA). By contrast, the linkage between high concentrations of ammonium and decomposition of soil organic matter, which ubiquitously contained nitrogen, suggested that mineralization of soil organic nitrogen compounds is the primary mechanism for the enrichment of ammonium in deeper groundwaters.

Pronounced temporal changes in soil microbial community and nitrogen transformation caused by benzalkonium chloride

As one typical cationic disinfectant, quaternary ammonium compounds (QACs) were approved for surface disinfection in the coronavirus disease 2019 pandemic and then unintentionally or intentionally released into the surrounding environment. Concerningly, it is still unclear how the soil microbial community succession happens and the nitrogen (N) cycling processes alter when exposed to QACs. In this study, one common QAC (benzalkonium chloride (BAC) was selected as the target contaminant, and its effects on the temporal changes in soil microbial community structure and nitrogen transformation processes were determined by qPCR and 16S rRNA sequencing-based methods. The results showed that the aerobic microbial degradation of BAC in the two different soils followed first-order kinetics with a half-life (4.92 vs. 17.33 days) highly dependent on the properties of the soil. BAC activated the abundance of N fixation gene (nifH) and nitrification genes (AOA and AOB) in the soil and inhibited that of denitrification gene (narG). BAC exposure resulted in the decrease of the alpha diversity of soil microbial community and the enrichment of Crenarchaeota and Proteobacteria. This study demonstrates that BAC degradation is accompanied by changes in soil microbial community structure and N transformation capacity.

Experimental and Simulation Research on the Process of Nitrogen Migration and Transformation in the Fluctuation Zone of Groundwater Level

The fluctuation of groundwater causes a change in the groundwater environment and then affects the migration and transformation of pollutants. To study the influence of water level fluctuations on nitrogen migration and transformation, physical experiments on the nitrogen migration and transformation process in the groundwater level fluctuation zone were carried out. A numerical model of nitrogen migration in the Vadose zone and the saturated zone was constructed by using the software HydrUS-1D. The correlation coefficient and the root mean square error of the model show that the model fits well. The numerical model is used to predict nitrogen migration and transformation in different water level fluctuation scenarios. The results show that, compared with the fluctuating physical experiment scenario, when the fluctuation range of the water level increases by 5 cm, the fluctuation range of the nitrogen concentration in the coarse sand, medium sand and fine sand media increases by 37.52%, 31.40% and 21.14%, respectively. Additionally, when the fluctuation range of the water level decreases by 5 cm, the fluctuation range of the nitrogen concentration in the coarse sand, medium sand and fine sand media decreases by 36.74%, 14.70% and 9.39%, respectively. The fluctuation of nitrogen concentration varies most significantly with the amplitude of water level fluctuations in coarse sand; the change in water level has the most significant impact on the flux of nitrate nitrogen and has little effect on the change in nitrite nitrogen and ammonium nitrogen, and the difference in fine sand is the most obvious, followed by medium sand, and the difference in coarse sand is not great.

The long-term decomposition of wild animal corpses leads to carbon and phosphorus accumulation and disturbs the ecological succession of the denitrification community encoded by narG

Corpse decomposition is a key process of the biogeochemical cycle in natural ecosystems, in which denitrification takes part. Denitrification is the process of nitrogen transformation which converts nitrate into nitrogenous gas. Nevertheless, the response and succession patterns of the denitrification communities during cadaver decomposition are largely unknown. To address this gap, we investigated the succession of the narG-encoding denitrifying communities during the decomposition process of wild animals (plateau pika) corpses using high-throughput Miseq sequencing. Our results showed that soil TP significantly accumulated in response to long-term cadaveric decomposition and could not be restored to control levels. But the abundance of many genera (e.g., Thiohalorhabdus, Acidovorax, Caballeronia) exhibited quadratic curvilinear recovery patterns during the cadaver decomposition. The alpha diversity also showed strong resilience and resistance to the cadaver decomposition. However, corpse decay led to the structural separation of the denitrifying communities with succession and increased the temporal turnover rate of the narG-type denitrifiers. The migration of the narG denitrification community was gradually limited during the cadaver decomposition. Moreover, the networks of the cadaver groups were more connected and complex than those of the control groups. Incubation time, cadaver decomposition, total carbon, and ammonium nitrogen were the most important factors driving the narG denitrification communities. These results suggest that denitrifying communities have a strong response to the decomposition of wild animal carcasses in soil habitats. This experiment provides a new view of the effect of wild animal corpse decomposition on the succession of denitrifying communities.

Chronic effects of cerium dioxide nanoparticles on biological nitrogen removal and nitrous oxide emission: Insight into impact mechanism and performance recovery potential

The influence of cerium dioxide nanoparticles (CeO2 NPs) on biological nitrogen removal and associated nitrous oxide (N2O) emission has seldom been addressed yet. Herein, the chronic effect of CeO2 NPs on the nitrogen transformation processes during wastewater treatment and the impacted system's self-recovery potential after CeO2 NP stress removal were investigated. CeO2 NP of 10–50 mg/L induced significant declines of the ammonia nitrogen (NH4+-N) and the total nitrogen removal efficiencies, but triggered the nitrite accumulation and the N2O emission. The N2O reductase (NOS) activity was negatively correlated with the N2O emission level, and the inhibition of NOS activity under CeO2 NP stress was probably due to the depressions of the sludge denitrifiers' metabolic activities. The NH4+-N removal efficiency was successfully regained after the recovery period although the N2O emission level was still higher than the pre-exposure period, which was probably due to the residual CeO2 NPs inside the activated sludge.

Soil dissolved organic matters mediate bacterial taxa to enhance nitrification rates under wheat cultivation

Studies have shown that dissolved organic matters (DOMs) may affect soil nutrient availability to plants due to their effect on microbial communities; however, the relationships of soil DOM-bacterial community-N function in response to root exudates remains poorly understand. Here, we evaluated the DOM composition, bacterial taxonomic variation and nitrogen transformation rates in both acidic and alkaline soils, with or without the typical nitrate preference plant (wheat, Triticum aestivum L.). After 30 days' cultivation, DOM compositions such as sugars, amines, amino acids, organic acid, and ketone were significantly increased in soil with wheat vs. bare soil, and these compounds were mainly involved in nitrogen metabolism pathways. Soil core bacterial abundance was changed while bacterial community diversity decreased in response to wheat planting. Function prediction analysis based on FAPROTAX software showed that the bacterial community were significantly (p < 0.05) affiliated with nitrification and organic compound degradation. Additionally, db-RDA and VPA analysis suggested that the contribution of soil DOM to the variance of bacterial community was stronger than that of soil available nutrients. Furthermore, the N-transformation related bacteria like Burkholderiales and ammonia-oxidizing bacteria (AOB) were positively correlated with soil gross nitrification rate, confirming that the soil N transformation was enhanced in both acidic and alkaline soils. Our results provide insight into how soil DOM affects the community structure and function of bacteria to regulate the process of nitrogen transformation in plant-soil system.

Variations of Bacterial and Diazotrophic Community Assemblies throughout the Soil Profile in Distinct Paddy Soil Types and Their Contributions to Soil Functionality.

ABSTRACTSoil microbiota plays fundamental roles in maintaining ecosystem functions and services, including biogeochemical processes and plant productivity. Despite the ubiquity of soil microorganisms from the topsoil to deeper layers, their vertical distribution and contribution to element cycling in subsoils remain poorly understood. Here, nine soil profiles (0 to 135 cm) were collected at the local scale (within 300 km) from two canonical paddy soil types (Fe-accumuli and Hapli stagnic anthrosols), representing redoximorphic and oxidative soil types, respectively. Variations with depth in edaphic characteristics and soil bacterial and diazotrophic community assemblies and their associations with element cycling were explored. The results revealed that nitrogen and iron status were the most distinguishing edaphic characteristics of the two soil types throughout the soil profile. The acidic Fe-accumuli stagnic anthrosols were characterized by lower concentrations of free iron oxides and total iron in topsoil and ammonia in deeper layers compared with the Hapli stagnic anthrosols. The bacterial and diazotrophic community assemblies were mainly shaped by soil depth, followed by soil type. Random forest analysis revealed that nitrogen and iron cycling were strongly correlated in Fe-accumuli stagnic anthrosol, whereas in Hapli soil, available sulfur was the most important variable predicting both nitrogen and iron cycling. The distinctive biogeochemical processes could be explained by the differences in enrichment of microbial taxa between the two soil types. The main discriminant clades were the iron-oxidizing denitrifier Rhodanobacter, Actinobacteria, and diazotrophic taxa (iron-reducing Geobacter, Nitrospirillum, and Burkholderia) in Fe-accumuli stagnic anthrosol and the sulfur-reducing diazotroph Desulfobacca in Hapli stagnic anthrosol.IMPORTANCE Rice paddy ecosystems support nearly half of the global population and harbor remarkably diverse microbiomes and functions in a variety of soil types. Diazotrophs provide significant bioavailable nitrogen in paddy soil, priming nitrogen transformation and other biogeochemical processes. This study provides a novel perspective on the vertical distribution of bacterial and diazotrophic communities in two hydragric anthrosols. Microbiome analysis revealed divergent biogeochemical processes in the two paddy soil types, with a dominance of nitrogen-iron cycling processes in Fe-accumuli stagnic anthrosol and sulfur-nitrogen-iron coupling in Hapli stagnic anthrosol. This study advances our understanding of the multiple significant roles played by soil microorganisms, especially diazotrophs, in biogeochemical element cycles, which have important ecological and biogeochemical ramifications.

Nitrate source apportionment and risk assessment: A study in the largest ion-adsorption rare earth mine in China

Nitrate (NO3−) pollution in water bodies has received widespread attention, but studies on nitrogen transformation and pollution risk assessment are still limited, especially in rare earth mining areas. In this study, surface and groundwater samples were collected from the largest rare earth mining site in southern China, and analyzed for the hydrochemical and stable isotopic characteristics. The results showed that the NO3− concentrations ranged from 1.61 to 453.11 mg/L, with 35% of surface water and 53.3% of groundwater samples exceeding the WHO standard (i.e., 50 mg/L). Health risk assessment showed that 31.4% of the water samples had a moderate to high non-carcinogenic risk, and the high-risk areas were concentrated in rare earth mining regions. Additionally, adults were more vulnerable to the non-carcinogenic health risks than children. The high variability of δ15N–NO3- (from −6.43 to 17.09‰) and δ18O–NO3- (from −7.91 to 22.79‰) showed that NO3− was influenced by multiple nitrogen sources and transformation processes. Hydrochemistry and isotopic evidence further indicated that NO3− was primarily influenced by nitrification and hydraulic connection between surface and groundwater. The results of the Bayesian mixing model showed that about 70% of NO3− originated from mine drainage and soil N in the rare earth mining area, while more than 90% of NO3− originated from fertilizer, soil N, and manure and sewage in rural and urban areas in the middle and downstream. This study suggests reducing anthropogenic nitrogen discharge (e.g., leaching agents and fertilizer inputs) as the primary means of NO3− pollution control with biogeochemical processes (e.g., denitrification) to further reduce its pollution.

The mechanisms and potentially positive effects of seven years of delayed and wetter wet seasons on nitrous oxide fluxes in a tropical monsoon forest

Tropical forest soils contribute to global warming and ozone depletion due to large nitrous oxide (N2O) emissions. However, it is unknown whether the soil N2O fluxes will change under ongoing precipitation regime changes. In this study, two typical precipitation regimes were simulated in a tropical monsoon forest for seven years: delayed wet season (DW) and wetter wet season (WW). Although we did not find significant effects of the precipitation changes on soil N2O fluxes annually or seasonally, significant increases in soil N2O emission were observed in treatment months for DW and WW. Importantly, the underlying mechanisms for their effects were different. For the DW treatment, the increase in soil N2O emission was attributed to the increase in abundance of nitrifying bacteria (amoA), which provide nitrate for N2O production, and to the changed microbial structure of denitrifying bacteria (nosZ), which reduce N2O consumption. For the WW treatment, the stimulation of soil N2O emission was attributed to the relatively large increase in N2O production via an increased abundance of denitrifying bacteria (nirS) compared with the increased N2O consumption via increased abundance of N2O reducing bacteria (nosZ). If the projected precipitation regimes of delayed or wetter wet seasons last for more than two months, the N2O emitted from tropical forest soils has the potential to significantly increase due to specific microbial nitrogen transformation processes. This increase implies that the contribution of tropical forests to global warming and ozone depletion would increase, depending on precipitation regimes.

Field Study of the Road Stormwater Runoff Bioretention System with Combined Soil Filter Media and Soil Moisture Conservation Ropes in North China

Growing concerns about urban runoff pollution and water scarcity caused by urbanization have prompted the application of bioretention facilities to manage urban stormwater. The purpose of this study was to evaluate the performance of proposed bioretention facilities regarding road runoff pollutant removal and the variation characteristics of the media physicochemical properties and microbial diversity in dry-cold regions. Two types of bioretention facilities were designed and then constructed in Tianjin Eco-city, China, on the basis of combined soil filter media screened by a laboratory-scale test with a modified bioretention facility (MBF) containing soil moisture conservation ropes. Redundancy analysis was performed to evaluate the relationships between the variation in media physicochemical properties and microbial communities. An increase in media moisture could promote an increase in the relative abundance of several dominant microbial communities. In the MBF, the relatively low nitrate-nitrogen (NO3-N) (0.75 mg/L) and total nitrogen (TN) (4.71 mg/L) effluent concentrations, as well as better removal efficiencies for TN and NO3-N in challenge tests, were mainly attributed to the greater relative abundance of Proteobacteria (25.2%) that are involved in the microbial nitrogen transformation process. The MBF also had greater media microbial richness (5253 operational taxonomic units) compared to the conventional bioretention facility and in situ saline soils. The results indicate that stormwater runoff treated by both bioretention facilities has potential use for daily greening and road spraying. The proposed design approach for bioretention facilities is applicable to LID practices and sustainable stormwater management in other urban regions.