Life Processes Research Articles

Highly selective Zn2+ near-infrared fluorescent probe and its application in biological imaging

Zn2+ plays a vital role in regulating various life processes, such as gene expression, cell signaling, and brain function. In this study, a near-infrared fluorescent probe AXS was synthesized to detect Zn2+ with good fluorescence specificity, high selectivity, and high sensitivity; the detection limit of Zn2+ was 6.924 × 10−11 M. The mechanism of Zn2+ recognition by the AXS probe was investigated by 1H nuclear magnetic resonance titrations, UV–visible spectroscopy, fluorescence spectroscopy, Fourier-transform infrared spectroscopy, and high-resolution mass spectrometry. Test paper experiments showed that the AXS probe could detect Zn2+ in real samples. In addition, quantitative and qualitative detection of Zn2+ in common foodstuffs was achieved. For portable Zn2+ detection, a smartphone detection platform was also developed based on the AXS probe. Importantly, the AXS probe showed good bioimaging capabilities in Caenorhabditis elegans and mice.

Implications of m5C modifications in ribosomal proteins on oxidative stress, metabolic reprogramming, and immune responses in patients with mid-to-late-stage head and neck squamous cell carcinoma: Insights from nanopore sequencing

BackgroundHead and Neck Squamous Cell Carcinoma (HNSCC) is a malignancy characterized by a high incidence and recurrence rate. 5-methylcytosine (m5C) RNA modification is a common alteration affecting cancer progression; however, how m5C operates within the tumor microenvironment of HNSCC remains to be elucidated. MethodsWe conducted Nanopore sequencing on 3 pairs of cancer and paracancerous tissues from mid- and late-stage HNSCC, obtaining 132 upregulated genes (transcriptomically upregulated, m5C elevated) and 129 downregulated genes (transcriptomically downregulated, m5C reduced). Subsequent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed; a differential gene interaction network (PPI) was constructed, revealing the interactions of each gene with others in the network. Co-expression analysis was performed on the genes within the PPI, unveiling their expression and regulatory relationships. Through GSVA analysis, variations in related pathways under different states were identified. Furthermore, results of m5C in lncRNA were screened, followed by target gene prediction. ResultsSequencing results from the 3 pairs of mid- and late-stage HNSCC cancer and paracancerous tissues demonstrated that RPS27A, RPL8, and the lncRNAs including differentiation antagonizing nonprotein coding RNA (DANCR), DCST1 antisense RNA 1 (CCDC144NL-AS1), Growth Arrest-Specific Transcript 5 (GAS5), Nuclear Paraspeckle Assembly Transcript 1 (NEAT1), and Small Nucleolar RNA Host Gene 3 (SNHG3), etc., under m5Cregulation, have close connections with surrounding genes. The differentially m5Cmodified genes are primarily involved in ribosomal protein synthesis, oxidative stress response, metabolic reprogramming, immunity, and other life processes; pathways like mitochondrial protein import and photodynamic therapy induced unfolded protein response are upregulated in the tumor, while pathways, including the classic P53, are suppressed. Analysis on m5C-regulated long non-coding RNAs (lncRNAs) revealed tight associations with RPS27A and RPL8 as well. ConclusionOur study identifies the key factors and signaling pathways involving m5C in HNSCC. The findings suggest that ribosome-related genes might regulate ribosomal protein synthesis, oxidative stress response, metabolic reprogramming, and immune response through m5C RNA modification by means like hypoxia and ferroptosis, thereby playing a pivotal role in the onset and progression of HNSCC. Hence, attention should be paid to the role of ribosomes in HNSCC. These findings may facilitate the precision and individualized treatment of patients with mid- and late-stage HNSCC in clinical settings.

The Experience of Cervical Cancer Patients Undergoing Hysterectomy: A Qualitative Study.

Due to different social and cultural backgrounds, cervical cancer patients' experience of the treatment process and quality of life after treatment will be different. This study sought to gain in-depth understanding of the experiences of Chinese cervical cancer patients as regards their quality of life and physical symptoms. Semi-structured interviews were used to collect data. We recruited 15 women with cervical cancer in eastern China for in-depth interviews. All data were entered into the NVivo 12 software program for analysis. Four themes emerged from the data: (a) uncertainty; (b) physical suffering; (c) psychological pressure; and (d) challenges of marriage and family. Cervical cancer patients showed concerns about the disease itself and the physical discomfort it causes, as well as changes in social relations. Health professionals need to talk about these issues and develop strategies to address them accordingly.

Cardiac Rehabilitation in Geriatric Individuals

Background: The aim of the study is a current review in the field of cardiac rehabilitation in geriatric individuals. Methods: Literature searches were conducted using PubMed, Embase and Scopus. Additional articles were identifed by searching reference lists. Results: Aging is a physiological condition in the normal process of life and leads to changes in all organs and systems. The aging process significantly affects the cardiovascular, pulmonary, musculoskeletal, and metabolic systems. Cardiac rehabilitation provides numerous psychological and physiological benefits to older adults. All comorbidities, frailty status, and social and financial factors should be comprehensively evaluated while planning cardiac rehabilitation programs that consist of the main components of exercise training, risk factor management, and psychosocial counseling in older adults. Conclusions: Regular participation in cardiac rehabilitation programs can reduce morbidity and mortality rates and improve cardiorespiratory fitness and overall health outcomes by modifying coronary artery disease risk factors in older adults with chronic conditions, including cardiovascular disease, stroke, and respiratory disease

Photochemical regulatory strategies for nucleic acid function and their biomedical applications

AbstractNucleic acids are not only essential biomolecules that drive critical life processes such as growth, development, reproduction, inheritance, and mutation, but also serve as significant markers for disease diagnosis, pathogen identification, and cancer screening. Nevertheless, several challenges have hindered the widespread use of nucleic acids in biomedicine, such as susceptibility to degradation, limited cellular uptake efficiency, potential toxicity, and uncontrollable activity. Photo‐regulation offers an effective solution to address these challenges. It allows for the precise control of nucleic acid structure and function and enhances the stability and safety of their application in biomedicine. In this review, we systematically review the structural characteristics of the three primary photosensitive groups commonly used in the regulation of nucleic acid molecules (i.e., photocleavable molecules, photoisomerization molecules, and photo‐crosslinking molecules) under light irradiation. Subsequently, recent research advances in the development and application of photo‐modulation strategies based on these photosensitive molecules in antisense oligonucleotides, RNA interference, nucleic acid amplification, and CRISPR/Cas systems are outlined. Finally, we discuss the challenges faced in the widespread application of these photo‐regulatory strategies and outline potential future directions for their development.

Transcriptome Analysis on the ALAN-Induced Zebrafish Ovary: Desynchronization of Life Processes and Initiation of Diseases

Objective: The effect of artificial light at night (ALAN) on “transcriptome” is prominent owing to its capacity for “desynchronization” of organismal physiology. Light influences the circadian rhythm. This study aims to explore the ALAN-induced ovarian transcriptome of zebrafish for desynchronization of life processes. Methods: Four experimental conditions were set up for female zebrafish: one normal 12-hour light and 12-hour dark (LD) cycle, and three continuous exposures to ALAN for one week (LLW), one month (LLM), and one year (LLY). The whole transcriptome data analysis of the ALAN-exposed samples was then compared with the normal sample using RNA-Seq, followed by exploratory analyses. Results: The analysis revealed two different patterns of expression of genes where LLW and LLM differ with LLY samples in comparison to LD. Compared to LD, downregulation of the predicted hub genes was observed in all treatments; ribosome and oxidative phosphorylation pathways were enriched. LLY vs. LD contrast depicts the enrichment of three more pathways—RNA polymerase, adhesion junction, and signaling. The gene ontology (GO) enrichment portrays more prevalent biological processes in LLW vs. LD and LLM vs. LD than in LLY vs. LD. Contrast-wise disease annotation represents neoplasms as the most prevalent; disease enrichment denotes the major class of neoplasm, carcinoma, coupled with intellectual disability, global developmental delay, and seizures. Conclusion: Our study displayed desynchronization of various genes and pathways leading to the initiation of diseases, for the first time in zebrafish. Even though, this data shows that ALAN is a serious threat, further research is needed to determine the intensity and the duration of ALAN which might cause potential repercussions.

Metabolic and Immune Systems Are the Primary Tenets of Biological Life

A theory is much broader than a hypothesis or a conjecture if it can provide explanations for a wide range of observations while remaining robust to challenges through experimentation. There is a paucity of such theories in biology. The main motivation behind this research was to propose a broad theory of biological life and to explore whether it can be used as a framework for understanding health and disease conditions. A conceptual and theoretical approach was followed for this purpose. Extensive exploration and analysis of the biomedical literature in a non-systematic but purposeful manner were undertaken. They were then extrapolated using a top-down approach to identify the fundamental systems mediating life processes. Two attributes must exist for the survival of any biological life form, including the ability to grow and the ability to self-protect. These two functions are performed by the metabolic system and immune system, respectively. It is proposed that all other processes and every entity in a life form are involved in conducting these two fundamental functions. It is inferred that both of these functions exhibit the universal characteristics of duality and complementarity, features that cut across disciplines. A given life form may thus be biased toward one of the two processes mediated by the immune system and metabolic system at different stages in its life history. Identifying factors that confer bias might provide a better understanding of biology with direct implications for medicine. It is proposed that life and disease could be the result of the interplay of immune and metabolic systems acting complementarily. Other physiological systems lend support to the balancing act between the immune and metabolic systems in a biological life form.

STUDY OF POLYPHENOLS CONTENT IN EXTRUDED MIX

Vegetable foods are rich not only in vitamins, fiber, healthy fats, but also contain another class of useful substances - polyphenols. Once in the human body, the polyphenols have a positive effect on all organs and life processes. These compounds have antioxidant activity, protecting our body from harmful environmental factors. The study presents data on the polyphenols content in extruded grain products with the addition of byproducts. The highest content of polyphenols is observed in extruded mix based on grains and 20 % grape pomace powder. Utilization of by-products improves the ecology of the country, and also allows the use of non-waste production.

An experimental study to assess the effectiveness of video-assisted childbirth education programme on knowledge regarding childbirth among primigravida mothers attending antenatal out patient department of a selected hospital in Bhopal.

Childbirth is a normal physiological process in a woman's life, yet it is a life-changing experience for the primigravida mothers. Boosting confidence regarding pregnancy and childbirth by enhancing knowledge is an important component of care. The aim was to assess the effectiveness of the video-assisted childbirth education programme on the knowledge of the primigravida mothers regarding childbirth. A quantitative research approach with a quasi-experimental non-equivalent control group design was adopted. Non-probability convenient sampling technique was used to select the 60 primigravida mothers who completed 34 weeks of gestation and are attending the Gynaec Outpatient Department (OPD) of a tertiary care hospital in Bhopal. The self-structured knowledge questionnaire was developed. On the first day of contact with the sample, pretest was taken from both groups. A tailor-made video was administered to the experimental group on the same day. Posttest was taken on the seventh day from both groups. Repeated measures analysis of variance (ANOVA) used to determine the effectiveness of video-assisted childbirth education programme revealed a significant interaction between the intervention and time, F (1,58) =29.398, P < .001. The mean difference in knowledge score over time was greater in the experimental group compared to the control group and the difference was statistically significant. The video-assisted childbirth education programme was effective in enhancing the knowledge of primigravida mothers regarding childbirth.

Anya’s Posttraumatic Growth Over Her Loss Trauma as Reflected in Natassa’s Critical Eleven

It has been commonly assumed that trauma only lead to a negative impact on its survivors. However, on another hand, trauma can bring positive changes. This situation is commonly said as post-traumatic growth (PTG). The PTG process takes a quite long time and each survivor has different capabilities and processes to achieve it. Anya, the main character in the novel of Critical Eleven, experiences the trauma of loss after her first baby dies. Since her baby left, Anya has been in a dark period. This research aims to determine the type of loss trauma experienced by Anya. As a trauma survivor, Anya's post-traumatic growth was also explained in this research. Two theories were utilized in this research. Freud's theory of mourning and melancholia was used to analyze the type of loss trauma that Anya experienced. Meanwhile, the post-traumatic growth theory (PTG) proposed by Tedeschi and Calhoun was used to determine Anya's post-traumatic growth domain. The primary data for this research were taken from the novel of Critical Eleven written by Ika Natassa. In addition, research related to traumatic loss and post-traumatic growth was used as supporting data. The research results show that the trauma of loss experienced by Anya is grief or mourning. Furthermore, the PTG domains found in Anya's personality reflected personal strength, inter-personal relationships, and spiritual change. In addition, this research also found that not all trauma survivors can achieve the five domains of post-traumatic growth due to the respective capabilities and life processes of each survivor.

DEFINITION OF THE MAIN PREREQUISITES FOR THE EMERGENCE OF THE TERM “SPACE ERGONOMICS”

The article discusses the main reasons for the formation of space ergonomics as a separate scientific discipline, since the knowledge used on the organisation of human activity in the surrounding techno-sphere on Earth differs significantly from the subject environment in outer space. The state of weightlessness dictates not only new rules in ergonomics, but also leads to the development of unique design solutions for a comfortable human stay in the space environment. Space ergonomics studies the laws of human life processes in the conditions of space flight. It is shown that the development of ergonometric requirements for the conditions of human professional activity in the space environment is an important factor for successful space exploration. The requirements include life support systems, safety, development of scientific and household equipment, design of the surrounding space, as well as consideration of psychological features of cosmonauts’ work.

Breast cancer symptom profile longitudinal changes: data mining study

ObjectivesIdentifying stable co-occurring symptoms in breast cancer (BC) patients during chemotherapy can improve symptom management and the treatment process. This study examines symptom cluster stability in Iranian BC patients receiving...

Modulating lipid bilayer permeability and structure: Impact of hydrophobic chain length, C-3 hydroxyl group, and double bond in sphingosine

Sphingosine, an amphiphilic molecule, plays a pivotal role as the core structure of sphingolipids, essential constituents of cell membranes. Its unique capability to enhance the permeability of lipid membranes profoundly influences crucial life processes. The molecular structure of sphingosine dictates its mode of entry into lipid bilayers and governs its interactions with lipids, thereby determining membrane permeability. However, the incomplete elucidation of the relationship between the molecular structure of sphingosine and the permeability of lipid membranes persists due to challenges associated with synthesizing sphingosine molecules. A series of sphingosine-derived molecules, featuring diverse hydrophobic chain lengths and distinct headgroup structure, were meticulously designed and successfully synthesized. These molecules were employed to investigate the permeability of large unilamellar vesicles, functioning as model lipid bilayers. With a decrease in the hydrophobic chain length of sphingosine from C15 to C11, the transient leakage ratio of vesicle contents escalated from ∼ 13 % to ∼ 28 %. Although the presence of double bond did not exert a pronounced influence on transient leakage, it significantly affected the continuous leakage ratio. Conversely, modifying the chirality of the C-3 hydroxyl group gives the opposite result. Notably, methylation at the C-3 hydroxyl significantly elevates transient leakage while suppressing the continuous leakage ratio. Additionally, sphingosines that significantly affect vesicle permeability tend to have a more pronounced impact on cell viability. Throughout this leakage process, the charge state of sphingosine-derived molecule aggregates in the solution emerged as a pivotal factor influencing vesicle permeability. Fluorescence lifetime experiments further revealed discernible variations in the effect of sphingosine molecular structure on the mobility of hydrophobic regions within lipid bilayers. These observed distinctions emphasize the impact of molecular structure on intermolecular interactions, extending to the microscopic architecture of membranes, and underscore the significance of subtle alterations in molecular structure and their associated aggregation behaviors in governing membrane permeability.

Interaction networks within disease-associated GαS variants characterized by an integrative biophysical approach

Activation of G proteins through nucleotide exchange initiates intracellular signaling cascades essential for life processes. Under normal conditions, nucleotide exchange is regulated by the formation of G protein–G protein-coupled receptor (GPCR) complexes. Single point mutations in the Gα subunit of G proteins bypass this interaction, leading to loss-of-function or constitutive gain-of-function, which is closely linked with the onset of multiple diseases. Despite the recognized significance of Gα mutations in disease pathology, structural information for most variants is lacking, potentially due to inherent protein dynamics that pose challenges for crystallography. To address this, we leveraged an integrative spectroscopic and computational approach to structurally characterize seven of the most frequently observed clinically-relevant mutations in the stimulatory Gα subunit, GαS. A previously proposed allosteric model of Gα activation linked structural changes in the nucleotide binding pocket with functionally important changes in interactions between switch regions. We investigated this allosteric connection in GαS by integrating data from variable temperature CD spectroscopy, which measured changes in global protein structure and stability, and molecular dynamics (MD) simulations, which observed changes in interaction networks between GαS switch regions. Further, saturation-transfer difference NMR (STD–NMR) spectroscopy was applied to observe changes in nucleotide interactions with residues within the nucleotide binding site. These data have enabled testing of predictions regarding how mutations in GαS result in loss or gain of function and evaluation of proposed structural mechanisms. The integration of experimental and computational data allowed us to propose a more nuanced classification of mechanisms underlying GαS gain-of-function and loss-of-function mutations.

Simultaneous Life Cycle Assessment and Process Simulation for Sustainable Process Design

Simultaneous Life Cycle Assessment and Process Simulation for Sustainable Process Design

Terrestrial site fidelity of maternal polar bears in western Hudson Bay

Seasonal migrations allow access to temporally varying resources and individuals may show fidelity to specific locations, particularly for important life processes such as reproduction. Polar bears ( Ursus maritimus) are a sea ice-dependent species that migrate between marine and terrestrial habitats, the latter being important for parturition and early cub rearing. However, fidelity to maternity dens is poorly understood. Using capture and telemetry data collected between 1979 and 2018, we assessed site fidelity of female polar bears in the Western Hudson Bay subpopulation in Manitoba, Canada. We compared within-individual inter-year distances between dens to between-individual distances via non-parametric bootstrapping, and used generalized additive models to assess how maternal age, years between denning events, and sea ice conditions affected fidelity. Within-individual distances were smaller than between-individual distances by approximately 18.7 km, indicating some evidence of fidelity. Inter-den distances increased with time between denning events, but no other variables significantly affected fidelity. Our findings suggest that western Hudson Bay polar bears may exhibit fidelity to areas within the larger denning region, but not to specific dens. Understanding the site fidelity of maternal polar bears has important implications for population dynamics, on-land spatial distributions, and conservation efforts aimed at preserving important habitat for reproduction.

Relationship between GTSE1 and Cell Cycle and Potential Regulatory Mechanisms in Lung Cancer Cells

The regulation of the cell cycle is essential for maintaining normal cellular function, especially in the development of diseases such as lung cancer. The cell cycle consists of four major phases (G1, S, G2 and M phases), which are characterized by a series of precise molecular events to ensure proper cell proliferation and division. In lung cancer cells, cell cycle dysregulation can lead to disordered proliferation and increased invasiveness of cancer cells. G2 and S-phase expressed 1 (GTSE1) is a regulatory protein found in the cytoplasm of the cell, which plays a key role in the cell cycle distribution of a wide range of cancer cells and is involved in life processes such as cell proliferation and apoptosis. GTSE1 affects cell cycle progression by interacting with cyclin-dependent kinase inhibitor 1A (p21) and maintaining the stability of p21, which in turn inhibits the activity of cyclin-dependent kinase 1/2 (CDK1/2). In addition, GTSE1 is also involved in the regulation of tumor protein 53 (p53) signaling pathway. With the assistance of mouse double minute 2 homolog (MDM2), GTSE1 is able to transport p53 from the nucleus to the cytoplasm and promote its ubiquitination and degradation, thus affecting cell cycle and cell death-related signaling pathways. This paper reviews the expression of GTSE1 in lung cancer cells and its effects on lung cancer, as well as its potential mechanisms involved in cell cycle regulation. .

Functional Prototissues Using Artificial Cells as Building Blocks and Their Biomedical Applications

AbstractThe construction of living systems from the bottom‐up helps to explore the complex processes of life and to understand their working mechanism. Prototissues, constructed using artificial cells as building blocks, mimic life systems at a high‐order tissue level, whilst artificial cells usually mimic living cells at the individual cell level. The 3D biomimetic prototissues demonstrate exceptional performances and collective functions, which reveal the working mechanisms of living tissues and hold promising potential for biomedical applications. This review systematically summarized the research progress of the field of prototissues. The engineering methods for fabricating two types of prototissues are introduced first, followed by the functions of prototissues including collective behaviors and signal communications, as well as their biomedical applications. The challenges and future trends are proposed at the end of the paper.

Pengungkapan Kinerja Lingkungan Instansi Pemerintah Daerah Melalui Laporan Keberlanjutan Berbasis Global Reporting Initiative

Laundry washing is a crucial process in daily life. Although automatic washing machines have simplified the washing process, utilizing advanced technology like fuzzy logic control can enhance the performance of smart washing machines to achieve optimal washing results. This research aims to implement fuzzy logic control using the Mamdani method on smart washing machines to improve efficiency and washing quality. The research methodology consists of several stages. Firstly, analyzing variables that affect the washing process, such as laundry load, fabric thickness, dirt level, and water temperature. Next, designing a Mamdani fuzzy logic control system by determining membership functions for each variable and creating fuzzy rules to link input and output variables. Lastly, evaluating washing results based on predefined input variables. The research findings demonstrate that implementing Mamdani fuzzy logic control on smart washing machines can significantly enhance washing quality and adaptively determine washing parameters to achieve cleaner washing outcomes. Therefore, integrating Mamdani fuzzy logic control into smart washing machines has the potential to improve overall washing performance. In conclusion, utilizing Mamdani fuzzy logic control on smart washing machines is an effective step towards enhancing efficiency and effectiveness in laundry washing. This research contributes to the development of more adaptive and environmentally-friendly smart washing machine technology and plays a crucial role in advancing fuzzy logic control technology for other smart household applications.

Predictive values of cervix length measurement based on transvaginal ultrasonography.

Pregnancy is an important process in women's life, which is widely concerned by women. In recent years, the incidence of premature delivery (PTD) becomes more and more higher due to the development of auxiliary reproduction and ovulation induction technologies and the changes of pregnant women's lifestyle and physical quality. PTD not only affects postpartum recovery and causes great physical pains, but it also has adverse effects on the birth state of neonates and even leads to neonatal death. The predictive values of cervix length (CL) measurement based on transvaginal ultrasonography (TVUS) and pathological examination of placenta for premature delivery (PTD) were investigated and the correlation between PTD and infection was analyzed. 120 pregnant women with PTD or high-risk factors for PTD admitted to The Affiliated Hospital of Southwest Medical University between February 2020 and March 2022 were included as the subjects and underwent pathological examination of placenta and TVUS for CL measurement. The final gestational age was set as the standard for the evaluation on the predictive values of pathological examination of placenta and TVUS. What's more, 36 subjects in PTD group and 84 in normal delivery group (control group) underwent pathological examination of placenta for the analysis of the correlation between PTD and infection. The joint inspection method showed significantly better sensitivity, specificity, PPV, and NPV compared to single CL or pathological examination of the placenta (P< 0.05). Among pregnant women, those with CL ⩽ 30 mm and positive placental pathology had a higher proportion compared to those with CL > 30 mm and negative placental pathology (P< 0.05). Furthermore, the incidence of Ureaplasma Urealyticum (UU), Chlamydia Trachomatis (CT), and Chorioamnionitis (CA) in vaginal discharge of the preterm delivery (PTD) group was significantly higher than that of the control group (P< 0.05). The combination of CL ⩽ 30mm and positive placental pathology could effectively predict PTD and placental infection was notably correlated with the occurrence of PTD.