Alkalization Process Research Articles

Effect of Alkalization on the Bacterial Cellulose Film Structure Produced Using the Pineapple Waste

Bacterial cellulose is natural polymers materials produced by Acetobacter xylinum with attractive physical properties because they are ordinary and uniform in structure. An alternative of cellulose from renewable source with more effective results to produce bacterial cellulose fibers. For this reason, the purpose of this paper is to show the effect of alkalization process on the bacterial cellulose film structure. The methods were the synthesis of bacterial cellulose using the extracts of pineapple waste. The pellicle product was treated using in the concentration of 0%, 1%, 5% and 10% then bacterial fiber films obtained from drying process of treated pellicle. Furthermore, the XRD and FTIR of bacterial cellulose were observed. The results of the structure of bacterial cellulose film was changed after a process in a high concentration of NaOH.

Changes in cocoa properties induced by the alkalization process: A review.

Alkalization, also known as "Dutching," is an optional, but very useful, step taken in the production chain of cocoa to darken its color, modify its taste, and increase natural cocoa solubility. Over the years, various attempts have been made to design new and more effective alkalization methods. Moreover, different authors have attempted to elucidate the impact of alkalization on the physicochemical, nutritional, functional, microbiological, and sensory characteristics of alkalized cocoa. The aim of this review is to provide a clear guide about not only the conditions that can be applied to alkalize cocoa, but also the reported effects of alkalization on the nutritional, functional, microbiological, and sensory characteristics of cocoa. The first part of this review describes different cocoa alkalization systems and how they can be tuned to induce specific changes in cocoa properties. The second part is a holistic analysis of the effects of the alkalization process on different cocoa features, performed by emphasizing the biochemistry behind all these transformations.

The Effect of Environmental Conditions on Pollution Deposition and Canopy Leaching in Two Pine Stands (West Pomerania and Świętokrzyskie Mountains, Poland)

This study analyzed the effect of atmospheric deposition on canopy leaching processes in stands predominantly composed of Scots pines. The research was conducted in two stands: the first located in the southwestern part of the Świętokrzyskie Mountains in the area known as Białe Zagłębie (Malik), and the second in the northwestern part of Poland in Western Pomerania (Czarne). The study was conducted in the hydrological years 2010–2013. The goal of the study was to assess the chemical transformation of precipitation as a result of various human activities. In Malik, the main factor that determined the physicochemical and chemical properties of throughfall was the cement/lime dust emitted by nearby industrial plants, which not only affected the quantity of deposit but also contributed to the alkalization process of throughfall. By contrast, the main source of pollution in Czarne, where local emitters are absent, was long-distance transport. We conducted a principal component analysis (PCA) in both stands that produced components representing local pollution (Malik) and long-distance transport (Czarne). For the stand in Czarne, sea aerosols were an important source of Na+ and Cl− ions in precipitation. In both stands, the highest enrichment ratio (ER) values were recorded for K+ ions. The contribution of leaching processes to the bulk deposition of K+ ions in Malik was 85.8%, whereas in Czarne, it was 73.8%. Regardless of the degree of anthropopressure, the intensity of quantitative and qualitative transformation in pine stands was comparable.

Optimizing the Cellulose Content of Shea Butter Bark Wood Fibre through Alkali Pretreatment for Composite Application

Objectives: This study was aimed to optimize the cellulose content (CC) increase with the percentage removal of hemicellulose (HC) and lignin content (LC) of shell butter bark wood fiber (SBBWF) by alkalization process. The truth remains that natural fibers have many uses to be employed in all human activities. The minimization of plant waste is a secret for close economy by effectively converting it to useful ventures. Methods/findings: The SBBWF was inserted in variant solutions for the concentration of sodium hydroxide (CSH) at 3–9 wt% and the soaking time (ST) for 6–18 h. The SBBWF before and after pretreatment were analyzed through gravimetric method and Fourier transform infra-red (FTIR) to determine its composition and organic substance with corresponding functionality, respectively. The optimization was examined using the central composite design (CCD), an option in response surface methodology (RSM). The results at optimal conditions indicated that the increment in CC with the reduction of HC and LC was 97.9988%, 44.5245%, and 45.716%, respectively. The factors at this junction were CSH and ST at 3 wt% and 18 h, respectively. The R2 values of CC, HC and LC had an approximation nearer to 1. The errors approximated by RSM and experimental readings were <0.43%. Application: Therefore, the modified SBBWF at this state is potential material in the component of composite manufacturing to be applied in domestic utilization. Keywords: Shell Butter Bark Wood Fiber, SBBWF, Wealth from Waste, Natural Source, Wood Fibre.

Optimization of NaOH concentration and trichloroacetic acid in bacterial carboxymethylation cellulose

The aims of this study were to obtain the optimal concentration of NaOH and trichloroacetic acid in the process of making CMC from bacterial cellulose. The experimental design used is response surface method. In the present study, NaOH as the variable X1 is concentration with a minimum limit (-1) of 30%, optimal limit (0) of 35% while the maximum limit (+1) of 40%. Variable X2 is the trichloroacetic acid concentration with a minimum limit (-1) of 25%, an optimal limit (0) of 30% and a maximum limit (+1) of 35%. Based on calculations using the program, expert design combination of treatments was obtained by 13 samples. The selection of the treatment above is based on the fact that in making CMC the alkalization process and carboxymethylation play an important role. The results showed variable optimum points of NaOH and Trichloroacetic Acid concentrations were 35.95% and 30.87%. The optimum point of the independent variable, the optimum response value of the substitution degree obtained was 0.897, water content of 20.08%, pH of 10.04 and viscosity of 17. 26%. In conclusion, that the best treatment of NaOH concentration on 35.95%, trichloroacetic acid on 30.87%, substitution degrees on 0.93, water content on 19.86%, pH on 10.17, and viscosity on 17.39 cPs.

The Effect of Alkalization on Carboxymethil Cellulose Synthesis from Stem and Peel Cellulose of Banana.

BACKGROUND:North Sumatra is one of the regions in Indonesia that produce bananas. Banana stems and peels contain cellulose and it can be isolated in nanofiber form. Carboxymethylcellulose is a cellulose derivative that undergoes an alkalization and etherification processAIM:This research was conducted to evaluate the alkalization effect on synthesis of carboxymethyl cellulose from stem and peel cellulose of banana.METHODS:Stem and peel of banana was extracted with NaOH 17.5% and the extract was synthesized to carboxymethyl cellulose (CMC). The synthesis of CMC was beginning with alkalization process in variation of NaOH concentration and the reaction was then etherificated with sodium monochloroacetate. CMC was characterized by FTIR and DS values were determined.RESULTS:FTIR spectra of synthesized CMC had different pattern compared to cellulose. It showed that an etherification reaction had been occurred in the cellulose compound. Spectra of CMC with variation in alkali concentration was not much different from one another. The synthesized CMC from stem and peel cellulose of banana had different degree of substitution (DS) values due to variate concentration of NaOH in alkalization.CONCLUSON:Alkalization on CMC synthesis affects the DS value of synthesized CMC. The increase DS value is proportional to the rise in NaOH concentration even though there is a boundary concentration to obtain the optimum DS value.

The Utilization of Carboxymethyl Cellulose (Cmc) from Groundnut (Arachis Hypogaea L) Cellulose as Stabilizer for Cow Milk Yogurt

The utilization of carboxymethyl cellulose (CMC) from groundnut (Arachis hypogaea L) cellulose as stabilizer for cow milk yogurt has been done in three steps. The first step was α-cellulose isolation from groundnut skin powder which was analysed with FTIR. The result was compared to FTIR analysis of commercial cellulose to verify the compound obtained is cellulose. The second step involves alkalization process using isopropanol and NaOH, carboxymethylation process with sodium chloroacetate (NaMCA), neutralization using CH3COOH 90% and ethanol, purification with aquadest and followed by centrifugation and addition of acetone to produce carboxymethyl cellulose. The CMC produced gave positive result in the qualitative anlysis, the FTIR spectrum was similar to commercial CMC and the degree of substitution obtained was 0.71. The last step is yogurt making process. In this stage, the CMC concentration added was varied from 0 – 0.5%. Then, the yogurt produced went through quality analysis such as syneresis, pH, viscosity, protein, fat content and organoleptic tests. The best result was obtained at the addition of 0.5% CMC concentration with 7.69% and 2.11% protein and fat content, pH 4.6, viscosity was 1676.01 x 102 cP, low syneresis with 90.66% stability and 22 days of storage life. Organoleptic result shows that yogurt with 0.3% CMC addition gave the best result with distinctive aroma and sourness, and rather thick texture. The panelists preferred such yogurt to others. The quality analysis for yogurt with CMC stabilizer still meets SNI standard. [Use 10 pt Times New Roman for the abstract body with single spacing and 10 pt spacing for the next heading. Left indent is 2 cm and right indent is 0 cm. Please write abstract paper in English with maximum length is 200 words.

Fabrication and tensile properties of bamboo micro-fibrils (BMF)/poly-lactic acid (PLA) green composite

In this research has been focused on the fabrication of green composites (GC) from emultion-type poly-lactic acid (PLA) biodegradable resin with bamboo micro-fibrils (BMF) reinforcement. BMF were extracted by combination methods: chemical solution and mechanical treatment. The chemical solution: alkalization of 5% (weight/volume) sodium hydroxide (NaOH) for 72 hours and bleaching of 2,8% volume natrium hypoclorit (NaClO) for 12 hours. And for mechanical treatment: high speed-blender for up to 30 minute along with the alkalization process. The chemical structure of the resulting cellulose BMF was studied by Fourier Transform Infra-Red (FTIR) spectroscopy. The alkalization and bleaching treatment were affected by the BMF cellulose content. Fabrication of green composites with BFC loading from 0%, 20%, 30%, 40% and 50% of mass were prepared using hot-press molding at constant temperature of 170°C, pressure of 10 MPa and time molding of 10 minutes. Tensile strength and elastic modulus was investigated using computerized Universal Testing Mechines HT-2402 with crosshead speed of 5.0 mm/min. The measurement finding showed that the tensile strength and elastic modulus were affected by the BMF content. The results obtained emphasize the application of bamboo micro-fibrils composites (BMC), as a potential materials in biodegradable based or green composites (GC).

Formation and Characterization of Polyphenol-Derived Red Chromophores. Enhancing the Color of Processed Cocoa Powders: Part 2.

Very recently, we described the formation of (+)-catechin- and (-)-epicatechin-derived polar chromophores by means of a cocoa alkalization process. In this study we focus on the generation of unpolar chromophores using model reactions via Dutching with softer alkaline conditions. UPLC-HR-ESI-TOF-MSe analysis and one-dimensional and two-dimensional NMR spectroscopy led to the unequivocal identification of dehydrodicatechin- and hydroxyxanthene-derived chromophores. The previously unknown 6- C-linked constitutional isomers of C-6'B/C-6D-(2 R,3 S)-dehydrodicatechin (3, 5) were first described, and furthermore, the dimers dehydrocatechin-C-6'B/C-8D-(2 S,3 S)-epicatechin (2) and dehydrocatechin-C-6'B/C-6D-(2 S,3 S)-epicatechin (4, 6) as well as the reddish-colored chromophores 8- C-xanthenocatechin (7), 8- C-xanthenoepicatechin (8), 6- C-xanthenocatechin (9), and 6- C-xanthenoepicatechin (10) were identified as new compounds. A LC-MS/MS method was developed to screen and quantify both classes of chromophores as well as their precursors in alkalized cocoa powders. The dehydrocatechin dimers showed degradation via the alkalization process; in contrast, the xanthenocatechins highlighted an increase in concentration with stronger alkalization, and, therefore, contribute to cocoa reddening. These results, together with those previously published, give a clear insight into the chemistry of polyphenol-derived chromophores generated by cocoa powder alkalization and enable a better understanding of chromophore formation mechanisms toward a more comprehensive color design of cocoa powders.

Formation and Characterization of Polyphenol-Derived Red Chromophores. Enhancing the Color of Processed Cocoa Powders: Part 1.

Although cocoa powder alkalization (Dutching) is a widely used industrial process to improve taste, dispersibility, and coloring of the final product, nevertheless knowledge about the compounds causing a change in coloring is fragmentary. By means of alkaline model reactions starting from the major cocoa polyphenol monomers, (+)-catechin or (-)-epicatechin, eight chromophores were derived from the first rearrangement product catechinic acid. LC-MS/MS analysis, one- and two dimensional-NMR, and electron paramagnetic resonance (EPR) spectroscopy led to the unequivocal identification of 6'-hydroxycatechinic acids (1a, 2a) and their radical states (1b, 2b), which were highlighted as main red chromophores. Six new dehydrocatechinic acid dimers (dehydrocatechinic acid-C-6'B/C-8D-(2 R,3 S)-catechin (3), dehydrocatechinic acid-C-6'B/C-6D-(2 R,3 S)-catechin (4, 5), dehydrocatechinic acid-C-6'B/C-8D-(2 R,3 R)-epicatechin (6), and dehydrocatechinic acid-C-6'B/C-6D-(2 R,3 R)-epicatechin (7, 8)) were also characterized as chromophores. 1-8 as well as their precursors were detected and quantified in alkalized cocoa powders via LC-MS/MS. With the increasing grade of alkalization, a decrease in catechin and epicatechin together with an increase in catechinic acid was observed. Compounds 1b, 2b, and 3-8 also showed a decrease in concentration by Dutching, which correlates to the accumulation of/to higher ordered chromophore oligomers and underlined the increase of the high molecular weight fraction. These findings give a first insight into the formation of structures causing the red coloring of cocoa, which offers the opportunity to optimize the alkalization process toward a better color design of cocoa powders.

Effect of Alkalization Treatment on The Tensile Strength and Interface Character Matrix-Fibber of Bamboo Petung (Dendrocalamus Asper) Reinforced Polyester Resin Composite

The purpose of this study is to determine the alkalization process effect of bamboo petung fiber (Dendrocalamus Asper) on the Tensile strength of Polyester composite reinforced with bamboo petung fiber. The alkalization process is expected to increase the bond between the automatic and the fiber which in turn can increase the tensile strength of the composite. In this study NaOH was used as an alkaline substance. NaOH is an alkali commonly used as a substance for the alkalization process in various natural fibers for natural composite reinforcing material. Alkalization was done by soaking the fiber into NaOH solution at room temperature and time variation for 1 hour, 2 hours, and 3 hours. After the alacalization process, the fiber was processed to be made into a composite with a polyester matrix with the direction of unidirectional fiber orientation. The process of making composites used vacuum infusion method. Before being used as reinforcing fibers, petung bamboo fiber composites were tested for single fiber tensile strength both after alkalization treatment and fiber before alkalization process. Composite tensile testing was conducted on all variations of both composites with fibers before Alkalization and composite after the alkalization process by using ASTM D638-03 standard. Scaning Electronics Microscope (SEM) was also conducted to observe the morphology of fibers before and after alkalization, besides that SEM was also used to determine the fracture shape of polyester composite reinforced with bamboo petung fiber. From the Tensile testing conducted on single fiber and composite, it was obtained results that either the single fibers strength or composites decreased with increasing NaOH soaking time.

Estimation of long-term Ph changes in lakes of the Caucasus using a bioindication method based on diatomaceous analysis

To analyze processes that may lead to long-term changes in pH, lake sediments from five small lakes in the Western and Central Caucasus were studied according to diatomaceous complexes from sediment cores. A proprietary principle of hydrological parameter unification was used to reconstruct numerical pH values. In isotopic dating experiments, a series of numerical pH values for 2000–130 years were generated for the lakes. These data indicate an absence of noticeable changes in pH in the lakes of the Western Caucasus and alkalization processes in the lakes of the Central Caucasus.

Cell-Surface-Anchored Ratiometric DNA Tweezer for Real-Time Monitoring of Extracellular and Apoplastic pH.

Precise and dynamic imaging of extracellular pH is one crucial yet challenging task for studying cell physiological and pathological processes. Here, we construct a DNA tweezer to dynamically monitor pH changes of cellular microenvironments. The DNA tweezer contains three key elements: a three-strand ssDNA-frame labeled with cholesterol to anchor it on the cell membrane, a pH-sensitive i-motif sequence in the middle to dynamically control the switch between the "open" and "closed" states of the DNA tweezer, and a pair of FRET fluorophores (rhodamine green and rhodamine red) on the two arms of the tweezer to reflect its state. With cholesterol, a natural component of cell membranes, as an anchoring element, the sensor exhibited high cell-membrane-insertion efficiency and low cytotoxicity. Using the i-motif as a sensing element, it can quickly and reversibly respond to extracellular pH in the pH range of 5.0-7.5 and further perform real-time imaging of cell-surface-pH changes with excellent spatial and temporal resolution. Moreover, apoplastic-pH change during the alkalization process of plant roots caused by rapid-alkalinization factor (RALF1) was directly detected by the sensor, demonstrating the potential applications of the sensor in cell biology, biomedical research, and plant-tissue engineering.

Comparative Assessment of the Effects of Soil Carbon and Nitrogen Amendment on Subsurface Soil pH, Eh and Sulfate Content of Acid Sulfate Soils

The harmful impacts of acid sulfate soils (ASS) on various ecosystems are diverse and management of the impacts needs ecosystem-specific strategies. The importance of subsurface soil carbon and nitrogen for amelioration of sulfuric soil and curtailing of sulfidic soil oxidation as an option for ASS management was investigated in this study. The results showed the mechanisms involved in amelioration of sulfuric soil and curtailing of sulfidic soil oxidation were dependent on amendment and the microbial ecology the resources were capable of supporting microbial metabolism. Glucose as a single nutrient source capable of engaging a few soil microbes was ineffective in ameliorating the sulfuric soil, compared to complex organic matter, which is a source of multiple nutrients and is capable of engaging a diversity of soil microbes. Addition of complex organic matter effectively ameliorated the sulfuric soil and prevented sulfidic soil oxidation. The alkalization processes was limited by resource availability and the processes ceased as resources got depleted. Polynucleotide analysis of at least one group of sulfate reducing bacteria (SRB, Desulfovibrio) showed that the concentrations of these polynucleotides is high when availability of resources is high and decreases as the resource is limited over time. A three-fold increase in RNA and a ten-fold increase in DNA concentrations of the target SRB were measured in soil amended with organic matter and incubated for four weeks; after six weeks, they decreased.

Synthesis and characterization of carboxymethyl cellulose (CMC) from salak-fruit seeds as anode binder for lithium-ion battery

Lithium-ion batteries are most popular battery for applications as portable electrical energy storage. Lithium-ion battery have some advantages compared to other battery such as have large specific energy capacity, light weight and long-life span. One of the components that contribute to the quality of Lithium-ion batteries is the binder material. Carboxymethyl Cellulose (CMC) is one of the most widely used binder materials other than PVDF. CMC can be synthesized from various organic materials such as empty bunches of palm, water hyacinth and salak-fruit seed. In this research, CMC is obtained from waste of salak-fruit seed (Salacca zalacca) and then used as anode binder in lithium-ion battery. The steps of CMC synthesis consist of cellulose isolation from salak-fruit seed, followed by alkalization process, carboxymethylation and finally the purification process. FTIR spectrum of CMC shows the successfully of CMC synthesis, the presence of strong absorption band at 1600 cm-1 and 1438 cm-1 is related to stretching vibration from the carboxyl group (COO-) and carboxyl group as it salts (COO-Na), respectively. The absorption in the region 1321–1400 cm-1 is due to symmetrical deformations of CH2 groups while broad absorption at around 3400 cm-1 is due to the stretching of the hydroxyl groups (-OH). Application CMC as binder of graphite anode with 5wt% CMC shows good surface profile and evenly distributed cavities with conductivity of about 0.815 S/cm.

EKSTRAKSI KARAGINAN RUMPUT LAUT MERAH (Kappaphycus alvarezii) DENGAN PERLAKUAN PERENDAMAN DALAM LARUTAN BASA

Semi-refined carrageenan are a type of carrageeanan product that have a low level of purity because it still contains a small amount of selulose within the carageenan. The purpose of this study is to find out the effect of the concentration of both NaOH and KOH towards rendemen, and the physical and chemical charactheristic of semi refined carrageenan made from kappaphycus alvarezii seaweed, and also to minimize the use of chemical product on SRC production process. The method used in this study is steaming method. The results are the rendemen from NaOH is 10% and KOH 14%. This proves that the concentration of alkali affects the amount of rendemen. The higher the amount of alkali used, the higher the amount of rendemen obtained. Water content obtained from the NaOH samples are 3,75%; while those from the KOH samples are 5%. The ash content of semi-refined carrageenan obtain from NaOH samples are 55,42% and KOH are 55,27%. For the pH level on semi-refined carrageenan obtain from the NaOH samples are 8,06; and KOH are 8,69. The alkali concentration greatly affects the amount of rendemen that is obtained because a higher concentration of alkali during the alkalization process will result in higher pH level, therefore the extration ability of alkali are increased.

Effect of Agitation in Alkalization Process on the Characteristics of Sodium Carboxymethyl Sago and Cassava Starches

Sodium carboxymethyl starch (Sodium CMS) is a modified starch prepared by two successive processes, alkalization and etherification. Alkalization will change the activated hydroxyl group of starch to more reactive alkoxide (St-O-), and then carboxymethyl group will substitute the hydroxyl group into sodium CMS. This research investigated the effect of agitation (1000 rpm of stirring and 4000 rpm of homogenization) in alkalization process to the modification of native starch into sodium CMS. Cassava and sago starches were mixed with sodium hydroxide (1.8 and 1.9 moles per mole anhydrous glucose units). The combination of NaOH and homogenizing gave the highest degrees of substitution for cassava (DS 0.73) and sago (DS 0.55) starches. The sodium CMS characteristics (paste clarity, water and oil absorption capacities, solubility, swelling power) were a function of mixing method but not on the amount of NaOH used.

The effect of processing methods on the improvement of tensile properties of Random Ramie Fiber – Reinforced Tapioca Starch Biocomposites

The disposal of Glass Fiber-Reinforced Plastic (GFRP) composites is becoming an environmental issue. To overcome the problem, biocomposites made of ramie fibers and tapioca starch might become an alternative to GFRP composites, due to their renewable properties, environmental friendly and easily degraded by nature. However, mechanical properties of fiber-reinforced starch based biocomposites are quite low due to high void content and poor fiber-matrix interface. In this research, the effect of the processing methods on tensile properties of tapioca starch - reinforced random ramie fiber biocomposites were studied. Ramie fiber reinforced tapioca starch biocomposites were fabricated by means of solution casting and compression molding technique. Random ramie fiber-reinforced tapioca starch biocomposites were succesfully made with the highest tensile strength and modulus elasticity of biocomposites were 18 MPa and 959 MPa respectively. The use of 1% NaOH alkalization process on ramie fiber increased tensile strength and modulus elasticity of biocomposites 64% and 54% respectively. Application of 120 °C compression temperature, increased tensile strength and modulus elasticity of biocomposites 18% and 74% respectively. Homogenous dispersion of ultimate fiber increased the tensile strength up to 91%. However, the addition of glycerol decreased the tensile strength and modulus elasticity for 46% and 39% respectively.

Fluorescent Silicon Nanorods-Based Ratiometric Sensors for Long-Term and Real-Time Measurements of Intracellular pH in Live Cells.

Long-term and real-time investigation of the dynamic process of pHi changes is critically significant for understanding the related pathogenesis of diseases and the design of intracellular drug delivery systems. Herein, we present a one-step synthetic strategy to construct ratiometric pH sensors, which are made of europium (Eu)-doped one-dimensional silicon nanorods (Eu@SiNRs). The as-prepared Eu@SiNRs have distinct emission maxima peaks at 470 and 620 nm under 405 nm excitation. Of particular note, the fluorescence emission intensity at 470 nm decreases along with the increase of pH, while the one at 620 nm is nearly unaffected by pH changes, making Eu@SiNRs a feasible probe for pH sensing ratiometrically. Moreover, Eu@SiNRs are found to be responsive to a broad pH range (ca. 3-9), biocompatible (e.g., ∼100% of cell viability during 24 h treatment) and photostable (e.g., ∼10% loss of intensity after 40 min continuous UV irradiation). Taking advantages of these merits, we employ Eu@SiNRs for the visualization of the cytoplasmic alkalization process mediated by nigericin in living cells, for around 30 min without interruption, revealing important information for understanding the dynamic process of pHi fluctuations.

Preparation of micro-fibrillated cellulose from sorghum fibre through alkalization and acetylation treatments

Recently, the pollution due to non-degradable materials including plastics, has led to needs on the development of environmental-friendly material. Owing to its biodegradability nature, sorghum fibres are interesting to be modified with petro-polymer as a composite. These materials are also expected to reduce the impact of environmental pollution. Surface modification of sorghum through chemical treatment was aimed to enhanced crystalline part of micro-fibrillated cellulose, thus increased compatibility to petro-polymer, as mean to improve composite properties. The experiments were conducted by alkalization process (10% NaOH) followed by acetylation with acetic acid glacial and acetic anhydride (CH3CO2)2 with additions of 1 and 2 drops of 25% H2SO4. Fourier transform infra-red (FTIR) spectroscopy, field-emission scanning electron microscope (FE-SEM) and x-ray diffraction (XRD) were used to characterize the treated and untreated fibres. The results of investigation showed that the chemical treatments have effectively produced MFC with the smallest fibre size around 5.5 – 6.5 microns and reduced lignin and hemicellulose where the highest crystalline part up to 80.64% was obtained through acetate acid treatment of 17.4 M, followed acetic anhydride with 1 drop of H2SO4 addition. Based on the current results, it is promising that the synthesized composites can be improved for their compatibilities.