Background: Diabetes is a metabolic disease characterized by hyperglycemia because of defect in insulin secretion or action or both. Asymptomatic bacteriuria (ASB) is common in diabetics and elderly which predisposes them to severe urinary tract infections. Reports on prevalence, etiology, antibiotic susceptible pattern are sparse in southern India. This study aimed to determine the prevalence of asymptomatic bacteriuria in patients with type 2 Diabetes mellitus Material & Method: This cross sectional study was conducted among the diabetic patients presenting to inpatient and outpatient ward in department of general medicine AVMC&H with ailments other than urinary tract infection were included. patients with symptoms of UTI, vulvovaginitis, pregnancy, recent history of hospitalization, recent urinary tract instrumentation, use of antimicrobial in last 14 days or UTI in recent past were excluded. Mid stream urine samples were collected from consented subjects. Urine samples were processed and examined for the various pathogens using the standard microbiological procedures. The spectrum of organisms causing asymptomatic bacteriuria and their antibiotic susceptibility prole. All data were collected by the investigator and was analysed statistically to nd the level of signicance of the study using SPSS v21. Results: In the present study total of 150 diabetic patients fullling inclusion criteria are included, with mean age of 55.6±10yrs, among them 66 were female and 84 were male patients. The isolates found to be in 50 patients (33.33%) out of 150 patients. Among the isolates, majority were with Escherichia Coli 52%, followed with 22% showing Klebsiella pneumoniae, 16% with Enterococcus species, 6% with proteus and 2% with Coagulase negative staphylococcus. Conclusion:The present study document 33.33% of the patients showing the asymptomatic bacteriuria. Among the isolates Escherichia coli was the most common organism among the patients with ASB. The presence of isolates was also correlated with the poor glycaemic control among the study participants, as one of the risk factor for ASB. Most isolates showed multiple resistance, indicating the need to speed up sensitization against antibiotic abuse