In Brazil, the overpopulation of dogs and cats is growing exponentially, causing adverse public and environmental health problems. The multiplication of these stray animal’s results in an increase in abandonment and mistreatment, also facilitating the proliferation of zoonoses, causing impacts on human health. Surgical sterilization, linked to educational programs aimed at the population, promotes awareness among owners regarding responsible ownership and can largely solve these problems, especially through greater incentives from public policies. In view of the above, this study aimed to demonstrate the effectiveness of neutering programs for dogs and cats with the aim of controlling this population, focusing on the One Health approach. On November 15th, 16th and 17th, 2019, the Veterinários da Amazônia group carried out a free surgical sterilization action in the municipality of Salinópolis, Pará, using the minimally invasive technique with a snook hook for ovariohysterectomy in female felines and canines, and pre-scrotal orchiectomy in male canines, and scrotal in male felines, resulting in 98 sterilizations, 55.1% canines and 46.9% felines, demonstrating a greater demand for the castration of female animals. Based on this, it is estimated that approximately 1,152 births of puppies and kittens were presented annually by the sterilization campaign in the municipality of Salinópolis - PA, of which 576 were felines and 576 were dogs, representing an impact of approximately 16.52% on the feline community and approximately 6.27% on the canine community of the municipality. These data indicate that the campaign was of great importance in reducing the birth rate, contributing significantly to the control of the stray animal population and to the promotion of animal welfare in the region. However, for effective birth control, greater awareness of the population regarding responsible guardianship as a predominant factor in animal welfare and subsequently the reduction of abandoned dogs and cats is required.