Probiotic Food Products Research Articles

Cytotoxicity, metabolic enzyme inhibitory, and anti-inflammatory effect of Lentinula edodes fermented using probiotic lactobacteria.

We found that the fermented Lentinula edodes (FLE) products exhibited various differences in terms of proximate composition, free sugar, and amino acid. In particular, there were higher levels of ergosterol, and ergothioneine in FLE-Pediococcus pentosaceus (PP) and -Lactobacillus acidophilus (LA) than in the L. edodes (LE) products. The survival rates of lactic acid bacteria (LAB) strains on artificial gastric juice, artificial bile, or heat (50-60°C) were observed to vary from 60%-66%, 60%-66%, to 42%-79%, respectively. The FLE products up to 300μg/ml had no cytotoxicity on RAW264.7, AGS, and RBL-2H3 cells, but inhibited the activities of α-amylase, α-glucosidase, and pancreatic lipase, as well as the production of nitrite, IL-1β, IL-4, TNF-α, and prostaglandin E2 (PGE2) from lipopolysaccharide (LPS)-induced inflammatory response. Our findings suggest that FLE products have metabolic enzyme inhibitory and anti-inflammatory effects. PRACTICAL APPLICATIONS: Fermentation plays a critical role in improving the functional and nutritional properties of food. In addition, lactobacteria are the main microorganisms involved in the fermentation of food known to have a variety of biological activities. Therefore, the utilization of lactobacteria for research and development of mushroom food materials can be used as a key strategy to improve the biological activity characteristics of mushroom food materials and to increase their active ingredient content. The present results show that FLE products had promising inhibitory efficacies against the activities of obesity-related metabolic enzymes and LPS-induced inflammatory response. These suggest that FLE products have the potential to be developed as functional probiotic dietary supplements or food products.

Probiotic Delivery through Non-Dairy Plant-Based Food Matrices

Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. Traditionally, dairy products are the major and most popular probiotic carriers. At present, there is a growing demand for non-dairy probiotic products. Both fermented and non-fermented non-dairy plant-based food products are becoming highly appealing to both dairy and non-dairy consumers worldwide. Non-dairy plant-based food matrices such as fruits, vegetables, plant-based milk, cereals, and legumes have been used successfully in producing probiotic products with the minimum recommended viable probiotic numbers at the time of consumption. However, due to the exclusion of dairy, whether these food matrices can enhance the functional properties of probiotics such as gastrointestinal survival and immune-enhancing effects needs a thorough investigation. Hence, this review focuses on some of the popular non-dairy plant-based probiotic food products and their microbiological quality characteristics in terms of maintaining probiotic viability during product storage. Their gastrointestinal tolerance in these products, other functional properties, and product qualities have also been briefly discussed.

Perspectives of Bacillus coagulans MTCC 5856 in the production of fermented dairy products

Probiotic bacteria protect against gastroenteritis, promote toxin reduction, cholesterol reduction, improved digestion and good function, mineral bioavailability enhancement. Bacterial strains, including Lactobacillus and Bifidobacterium, are generally used in probiotic food production. However, they are weakly resistant to the stomach's aggressive environment and high temperature, which can result in significant loss of viable probiotic microorganisms. Application of spore-forming bacteria Bacillus coagulans MTCC 5856 allows developing an innovative natural dairy fermented product with high probiotic microorganism viability (p < 0.05) during the technological process, storage period, at different pH values, and in the presence of bile salts when examining in vitro. Due to the low caseolytic activity of Bacillus coagulans, the proper ratio and fermentation temperature with the use of auxiliary starter culture Streptococcus thermophilus were established. The addition of milk protein improves the rheological characteristics of the product. Bacillus coagulans and Streptococcus thermophilus maintained more than 8 Log10 CFU/g of viable microorganisms during the product storage within 60 days. The use of Bacillus coagulans allows increasing the fermented product's antioxidant activity versus the antioxidant activity of the product when Streptococcus thermophilus is used alone. The results showed that the fermented dairy product itself to be an excellent carrier of Bacillus coagulans.

Growth, survival, and metabolic activities of probiotics Lactobacillus rhamnosus GG and Saccharomyces cerevisiae var. boulardii CNCM-I745 in fermented coffee brews

Amidst rising demand for non-dairy probiotic foods, and growing interest in coffees with added functionalities, it would be opportune to ferment coffee brews with probiotics. However, challenges exist in maintaining probiotic viability in high-moisture food products. Here, we aimed to enhance the viability of the probiotic bacteria, Lactobacillus rhamnosus GG, in coffee brews by co-culturing with the probiotic yeast, Saccharomyces cerevisiae var. boulardii CNCM-I745. The yeast significantly enhanced the viability of L. rhamnosus GG, as bacterial populations beyond 7 Log CFU/mL were maintained throughout 14 weeks of storage at 4 and 25 °C. In contrast, the single culture of L. rhamnosus GG suffered viability losses below 6 Log CFU/mL within 10 weeks at 4 °C, and 3 weeks at 25 °C. Growth and survival of S. boulardii CNCM-I745 remained unaffected by the presence of L. rhamnosus GG. Volatile profiles of coffee brews were altered by probiotic metabolic activities, but co-culturing led to suppressed generation of diacetyl and ethanol compared to single cultures. Probiotic fermentation did not alter principal coffee bioactive compounds and antioxidant capacities; however, declines in peroxyl radical scavenging capacities were observed after ambient storage. Overall, we illustrate that yeasts are effective in enhancing probiotic bacterial viability in coffee brews, which may be useful in developing shelf stable probiotic food products.

Potential of Changchong pear (Pyrus pyrifolia Nakai cv. Changchong) to improve the growth and survival of probiotic lactobacilli strains

The application of most probiotics in the food industry has been hampered by their sensitivity to oxygen. Natural antioxidants have shown a broad prospect in counteracting the oxygen stress and improving the growth of probiotics in the context of oxygen stress.This study reported on a pear cultivar (Pyrus pyrifolia Nakai cv. Changchong), whose fruit is characterized by a lower browning rate compared to its counterparts. A moderate but persistent antioxidant pear juice was obtained and biochemical analysis suggested its potential to counteract the oxygen stress. Its effect on the growth and survival of the probiotic lactobacilli strains was investigated in the context of oxygen stress. The results indicated that Changchong pear juice is efficient in growth improvement as well as survival enhancement for the Lactiplantibacillusplantarum strain ST-III and other probiotic representatives of this species. Additionally, experiments performed with other probiotic lactobacilli strains showed that it is also effective on Lacticaseibacillusrhamnosus GG and Lactobacillusacidophilus NCFM, suggesting its potential in a broader sense. These results highlighted the potential of a moderate but persistent natural antioxidant in the production and preservation of probiotic foods, and offered a new perspective for protecting oxygen-sensitive probiotics from chronic oxygen stress.

Consumer awareness and willingness to purchase probiotic food and beverage products: a study of Sonipat district, Haryana

PurposeIn a developing country like India, consumers are shifting from high-energy diet to balanced nutritional diet, which enhances the metabolic, physiological, functional and health benefits of consumers. The growing self-care and health-embracing population made the probiotic sector as lucrative market for functional food manufacturers and suppliers. Therefore, an analysis is required to understand consumers’ knowledge and buying behavior regarding probiotic products for product innovation and development.Design/methodology/approachAwareness, knowledge and consumption patterns of probiotic products were investigated among randomly selected 306 consumers in the Sonipat city of Haryana, which comes in the proximity of national capital, i.e. Delhi, India. A structured questionnaire with exploratory characteristics was developed and face-to-face interviews were conducted as a part of data collection.FindingsThe inference is that there is immense potential for the probiotic fruit beverage market as a higher proportion of the population was interested in probiotic products delivered with fruits as a base matrix followed by dairy products. Further, government’s initiation and support are required not only for manufacturing such health-beneficial products having therapeutic value but also to spread awareness regarding the necessity of including such products in the meals of the growing children.Originality/valueThe study shows changing customer attitudes toward probiotic products and sheds light on consumers’ preference on the medium (food matrix) of probiotic delivery, thereby exploring market opportunities in the area of the functional food sector.

High-quality genome sequence assembly of R.A73 Enterococcus faecium isolated from freshwater fish mucus

BackgroundWhole-genome sequencing using high throughput technologies has revolutionized and speeded up the scientific investigation of bacterial genetics, biochemistry, and molecular biology. Lactic acid bacteria (LABs) have been extensively used in fermentation and more recently as probiotics in food products that promote health. Genome sequencing and functional genomics investigations of LABs varieties provide rapid and important information about their diversity and their evolution, revealing a significant molecular basis.This study investigated the whole genome sequences of the Enterococcus faecium strain (HG937697), isolated from the mucus of freshwater fish in Tunisian dams. Genomic DNA was extracted using the Quick-GDNA kit and sequenced using the Illumina HiSeq2500 system. Sequences quality assessment was performed using FastQC software. The complete genome annotation was carried out with the Rapid Annotation using Subsystem Technology (RAST) web server then NCBI PGAAP.ResultsThe Enterococcus faecium R.A73 assembled in 28 contigs consisting of 2,935,283 bps. The genome annotation revealed 2884 genes in total including 2834 coding sequences and 50 RNAs containing 3 rRNAs (one rRNA 16 s, one rRNA 23 s and one rRNA 5 s) and 47 tRNAs. Twenty-two genes implicated in bacteriocin production are identified within the Enterococcus faecium R.A73 strain.ConclusionData obtained provide insights to further investigate the effective strategy for testing this Enterococcus faecium R.A73 strain in the industrial manufacturing process. Studying their metabolism with bioinformatics tools represents the future challenge and contribution to improving the utilization of the multi-purpose bacteria in food.

Antimicrobial activity of Satureja Khuzestanica Jamzad and Satureja bachtiarica Bunge essential oils against Shigella flexneri and Escherichia coli in table cream containing Lactobacillus plantarum LU5.

The essential oils (EOs) from Marzeh khuzestani (Satureja Khuzestanica Jamzad) and Marzeh bakhtiari (Satureja bachtiarica Bunge) were analyzed and their antibacterial activities on Shigella flexneri and Escherichia coli in probiotic table cream containing Lactobacillus plantarum LU5 were evaluated. Carvacrol (86.5%) was the main component of Marzeh khuzestani, but thymol (33.5%), carvacrol (14.2%), borneol (13.4%), and linalool (11.5%) were the major constituents of Marzeh Bakhtiari EOs. Marzeh khuzestani exhibited the highest antibacterial/bactericidal activity on the tested bacteria. EOs combination showed no interaction on the L. plantarum but a synergism effect to inhibit the pathogen strains observed. Agar diffusion assay showed the highest inhibitory effect on S. flexneri (32.7mm), E. coli (28.4mm), and L. plantarum (24.7mm) for the combination 2:1 Marzeh khuzestani:Marzeh Bakhtiari (p≤.05). The antibacterial activity of mixture of EOs in creams was evaluated and the sample contained of 1%k+1%b showed the highest antibacterial activity after day 10 of storage (by lowering the number of E. coli, S. flexneri, and L. plantarum to 2.3, 1.9, and 1.4 log CFU/g compared to control sample). Overall acceptability of creams slightly decreased by the increase in EOs addition and the highest acceptability score of 7.9 observed for the sample contained 0.5%k+0.5%b EOs. However, all treatments exhibited a high acceptance level that it confirms that the addition of EOs mixture had no effect on the sensorial attributes of the creams. The combination of tested EOs can be used as an antimicrobial agent in probiotic food products containing L. plantarum.

Quality evaluation of probiotic pasta produced with Bacillus coagulans GBI-30

Nowadays, the production of non-dairy and heat-treated probiotic foods has been gaining high interest. With this aim, due to its spore-forming ability Bacillus coagulans has been widely used in research studies. In this study, probiotic pasta production was accomplished with the addition of Bacillus coagulans GBI-30 spores into semolina. Samples were packed in zip-lock bag and stored at room temperature for 6 months. The microbiological characteristics, cooking behavior, texture, color and sensory attributes of pasta samples were investigated at 0 and 6 months of storage. Results showed that B. coagulans GBI-30 count of pasta samples decreased from 7.20 to 6.08 log CFU/g during cooking and storage showed statistically significant effect on B. coagulans GBI-30 count. At the end of the cooking in boiling (100 °C) distilled water for 8 min, B. coagulans GBI-30 did not negatively affect the sensorial quality of pasta according to the sensory panel of 7 experts. Therefore, it was concluded that the pasta can be considered as a probiotic product after 6 months of storage at room temperature due to the fact that B. coagulans GBI-30 count was above 6 log CFU/g.

Probiotic oats milk drink with microencapsulated Lactobacillus plantarum – an alternative to dairy products

Purpose Interest in probiotic food products has constantly increased due to the awareness on importance of gut microbiome; an increasing demand has encouraged the development of other matrices such as cereals, vegetable and fruit juices to deliver probiotics. The purpose of this paper is to standardize and evaluate a ready to serve probiotic oats milk drink fermented with microencapsulated Lactobacillus plantarum to be further used as a therapeutic module. Design/methodology/approach Fermentation of oats milk extract with microencapsulated L.plantarum was subjected to various trials in making it favourable for consumption and tested for sensory characteristics, physicochemical parameters, nutrient content, viable colony count and shelf life. Findings Fermented oats milk drink with 3% inoculum of microencapsulated L.plantarum was able to achieve desirable level of 2.5 × 108 and 2.3 × 108 colony forming units (CFU)/mL for spice and strawberry flavoured drink, respectively. Antioxidant property significantly increased after fermentation showing inhibitory effect against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (p = 0.05). Research limitations/implications Analysis of all the parameters were conducted only with three samples; this was the potential limitation identified in this study as large sample size always be a better representative of the results. Practical implications Spice and strawberry flavoured nondairy oats milk drink facilitated to be a suitable carrier for microencapsulated L. plantarum with good sensory attributes, low fat, moderate calorie, high fiber content, antioxidant potential and a shelf life of two-week period at 4°C. Originality/value The developed ready to serve, spice and strawberry flavoured nondairy oats milk drink with compactly packed functional components inclusive of beneficial probiotic organisms, ß-glucan and antioxidants can be prescribed as a therapeutic food for many clinical conditions and would serve as a good probiotic option for vegans.

Challenges of Probiotics Microencapsulation by Spray Drying Method

Introduction: In the recent years, attention to the use of probiotic drugs and probiotic food products, due to probiotic’s functional and health properties has been increased. The storage and the viability of the probiotics in the products and passing through the gastrointestinal tract was always a big challenge. Drying is one of the functional and important choices that can be used for stabilizing microbial species and makes the bacterial usage easy. Among different microencapsulation methods, spray drying is one of the important drying methods that can be used in industrial scale and size. Optimizing the effective parameters on the cultivation and separation of probiotic bacteria, the most important and challenging parameters of spray drying that have influence on probiotic products, and choose of the best wall materials for the cores, are the most important and challenging factors that are necessary for optimizing the microencapsulation in industrial scale. In this review article, in this review article, the factors affecting the survival and resistance of bacteria in the stage before entering the spray dryer and the factors affecting the survival of probiotic bacteria during the drying stage are discussed. In addition, the important criteria for selecting microfiber wall materials and the tests that are often performed on probiotic microfiber resulting from spray drying have been examined.

Quantitation of DNA reactive pyrrolic metabolites of senecionine – A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis

High hydrostatic pressure processing (HPP) is a non-thermal pasteurization technology which has already been applied in the food industries. Besides maintaining the food safety and quality, HPP also has potential applications in the enhancement of the health benefits of food products. This study examines the current progress of research on the use of HPP in the development of health foods. Through HPP, the nutritional value of food products can be enhanced or retained, including promotes the biosynthesis of γ-aminobutyric acid (GABA) in the food materials, retains immunoglobulin components in dairy products, increases resistant starch content in cereals, and reduces the glycemic index of fruit and vegetable products, which facilitates better control of blood glucose levels and decreases calorie intake. HPP can also be utilized as a hurdle technology in combination with existing processing technologies for the development of low-sodium food products and the maintenance of microbial safety, thereby lowering the risk of triggering cardiovascular disease. Additionally, HPP can be used to enhance the diversity of probiotic food products. Appropriate sporogenous probiotics can be screened and added to various high-pressure processed food products as a certain bacterial count is still retained in the products after HPP. As HPP causes physical damage to the structures of food products, it can also be used as a synergistic extraction technology to enhance the extraction efficiency of functional components, thereby reducing extraction time. By applying HPP in the extraction of functional components from food waste, the production costs of such components can be effectively reduced. This study provides a summary of the mechanisms by which HPP enhances the health benefits of food products and the current progress of relevant research. HPP possesses huge potential in the development of novel health foods and may provide an abundance of benefits to human health in the future. © 2019

INVESTIGATION ON QUALITY PROPERTIES OF TRADITIONAL BULK BREAD COVERED WITH PROBIOTICS AND SOYBEAN OIL EDIBLE COATING

Probiotic food products are available at the supermarket commercially, but probiotic bakery products are much less in evidence. In the present study, methyl cellulose (2%), whey protein concentrate (2%), corn starch (1%), and soybean oil at 2, 4, and 6% were used for coating layer on the bulked bread surface, and then the quality properties were studied. The results showed that Lactobacillus rhamnosus GG, as probiotic component of the coating, immobilized in corn starch, whey protein, and methyl cellulose films had enhanced viability throughout shelf-life. The probiotics remained viable for 4 days, maintaining high viable cell number levels. Adding soybean oil at 6% concentration enhanced texture, sensory properties, and image index during storage.

Novel encapsulation approach for Bifidobacterium subsp. lactis (BB-12) viability enhancement through its incorporation into a double emulsion prior to the extrusion process

Due to the shift towards healthy lifestyles, the food industry focuses on expanding the production of probiotic food products. Τhe incorporation of probiotic bacteria into the food matrix is challenging because various processes can damage cells’ viability. In this study, an innovative and advanced encapsulation approach combining the extrusion technique with the technique of double emulsification (W1/O/W2 emulsion system) is proposed for the protection of Bifidobacterium lactis (BB-12). Different W1/O/W2 emulsion formulations were investigated using various encapsulating agents as wall materials for satisfactory encapsulation of BB-12 cells. The probiotic cells were incorporated in the double emulsion prior to extrusion and their viability was evaluated by exposing them to different storage temperatures and various pH values, as well as to simulated gastrointestinal fluids. The results demonstrated that the viability of probiotics, when using the proposed encapsulation system, maintained above 6 log cfu g−1 during storage at 4 °C and −18 °C for 4 weeks, while the BB-12 cells encapsulated through conventional extrusion were decreased significantly (survival rate 46.8%) after 15 days of storage. Moreover, this novel system provides high survival rates during the in vitro simulation of the gastrointestinal system (up to 86.1%) and at various pH environments (up to 98.5%).

Application of edible films containing probiotics in food products

The trend towards functional foods and healthy diets encourages researchers to produce new probiotic food products with improved probiotic delivery methods. Recently, edible films have been applied as carriers for probiotics, improving their survival in the foods and the gastrointestinal tract. This article is structured as follows: a description of edible films as probiotic carriers is followed by an introduction of the materials used as edible films in three main chapters (1) hydrocolloids (polysaccharides and proteins), (2) lipids and (3) composites. Then, probiotic viability in all mentioned edible films is discussed in detail and the impact of prebiotic incorporation is mentioned. Microbial, physicochemical, and sensory properties of edible film containing probiotics in food matrices are reviewed. Finally, the application of probiotic edible films in different food products is described.

Retail refrigerated probiotic foods and their association with evidence of health benefits.

Probiotic usage in food is widespread and growing. The objective of this study was to determine the percentage of probiotic food products sold in the refrigerated section of retail grocery stores in the Washington DC area that we could link to evidence of any health benefit. We surveyed refrigerated sections of eight large grocery stores representing five national chains for probiotic products. Based on declared probiotic composition (strain and count) for each product, we searched PubMed for controlled trials that provided evidence of any health benefit. Our assessment showed that 49% (22 out of 45 distinct probiotic foods) could be linked to evidence supporting a health benefit. All products indicating strain composition could be linked to evidence. Our study suggests that consumers have a reasonable likelihood of purchasing a refrigerated probiotic food with evidence, but room for improvement exists.

Bioactive compounds produced by probiotics in food products

Probiotics and associated health benefits emerged as an area of increasing interest in the past few decades. Probiotics confer their beneficial health effects by different mechanisms of action such as preventing pathogen adhesion or colonization, the production of metabolites, and modulation of the immune system by production of immunoglobulins antibodies. The most frequently used probiotics belong to the genera Bifidobacterium and Lactobacillus. This review focuses on the health benefits of different bioactive compounds produced by probiotics, their mode of action and applications in food. However, viability of the microbial cells during processing, packaging, and storage of the food should be maintained in the foods supplemented with probiotic microorganisms.

Ethnic and industrial probiotic foods and beverages: efficacy and acceptance

Microbial fermentation is an indigenous process known to be adapted for centuries by different communities and folks for the improvement of the quality of the food. Quality enhancement of the food is mostly carried out by lactic acid fermentation and sometimes probiotic fermentation mainly for the elongation of shelf-life, nutritional value addition and improvement of the sensory property. Technological interventions have led to the commercial production of many indigenous probiotic foods and beverages. The article reviews varieties of ethnic and industrial probiotic food and their distribution throughout the globe. Also, the health perspective, the involvement of microorganisms and their impact on the development of the food product have been elucidated in the article. Moreover, bottlenecks and the direction of research and commercialization with regards to ethnic and industrial probiotic food are of crucial importance and are also discussed in this review.

Celiac therapy by administration of probiotics in food products: a review

Celiac disease (CD) is a systematic immune-mediated enteropathy induced in the most genetically predisposed people by exposure to prolamin proteins. The only effective strategy for inhibiting CD is a lifelong exclusion of gluten from patient’s diet; however, adherence to a gluten-free diet (GFD) could be difficult for them. Because of this challenge, there is a demand for novel therapies. The utilization of probiotics in CD patient’s diets has been proposed as a novel therapy for reducing CD symptoms. Probiotic strains can suppress the gliadin toxicity through some probable mechanisms such as enzymatic hydrolysis of toxic peptides derived from gliadin. The present review describes the recent scientific researches about mechanisms of suppressing gliadin by probiotics.

Fermented milk: The most popular probiotic food carrier.

Fermented milks are extensively produced and consumed all around the world. The production of these products is an old process that was used for extending the shelf life of milk. Nowadays, numerous traditional and industrial fermented milks with various texture and aroma can be found as an important part of human diet that exhibit several health benefits. In recent years, consumers' awareness about the effect of diet on health and tendency for consuming healthful food products directed manufacturers to develop functional foods. In this context, production of probiotic food products is a common approach. Fermented milks are suitable carrier for probiotics and their production and consumption can be a beneficial way for improving health status. For development of probiotic fermented milks, probiotic viability during fermentation and storage time, their interaction with starter cultures in the product as well as their effect on sensory properties of the product should be taken into account. This chapter describes different fermented milks, probiotics used in fermented milks, process of their production and quality aspects associated with these products.