Probiotic Food Products Research Articles

Fruit Juices as Alternative to Dairy Products for Probiotics’ Intake

In the context of functional foods and beverages, probiotic fruit juices offer a unique opportunity to combine probiotics’ health benefits with fruit juices’ nutritious properties. Since most probiotic food products are dairy products, fruit juices could be a viable alternative to dairy foods. Moreover, the variety of tastes with which these juices can be produced can expand the market for these products to consumers of all ages. Important challenges for the probiotic juice manufacturing industry are stability and sensory properties, which can be altered by probiotics or their metabolites. Any functional food must be healthy but still acceptable to consumers’ tastes. To evaluate the safety of these products, and improve the efficacy of probiotics in fruit juices, innovative technologies have been proposed, such as microencapsulation and the contemporary use of antioxidants.

Co-Encapsulation of Coffee and Coffee By-Product Extracts with Probiotic Kluyveromyces lactis.

Coffee and coffee by-products contain several chemical compounds of great relevance, such as chlorogenic acid (CGA), trigonelline, and caffeine. Furthermore, yeasts have been the target of studies for their use as probiotics because of their interesting biochemical characteristics. The combined administration of probiotic microorganisms with components that provide health benefits mediated by alginate encapsulation is an alternative that ensures the stability of cells and chemical compounds. In this context, the aim of this work was to co-encapsulate the probiotic yeast Kluyveromyces lactis B10 and extracts of green coffee beans, coffee silverskin, and PVA (black, green or immature, and sour coffee beans). The bioactive composition, antioxidant and antimicrobial activities of the extracts, microcapsule morphological characteristics and encapsulation efficiency, ability of the encapsulation to protect the yeast cells subjected to gastrointestinal conditions, and antioxidant activity of the microcapsules were evaluated. All the evaluated extracts showed antioxidant activity, of which PVA showed 75.7% and 77.0%, green coffee bean showed 66.4% and 45.7%, and coffee silverskin showed 67.7% and 37.4% inhibition of DPPH and ABTS•+ radicals, respectively, and antimicrobial activity against the pathogenic bacteria E. coli, Salmonella, and S. aureus, with high activity for the PVA extract. The microcapsules presented diameters of between 1451.46 and 1581.12 μm. The encapsulation efficiencies referring to the yeast retention in the microcapsules were 98.05%, 96.51%, and 96.32% for green coffee bean, coffee silverskin, and PVA, respectively. Scanning electron microscopy (SEM) showed that the microcapsules of the three extracts presented small deformations and irregularities on the surface. The K. lactis cells encapsulated in all treatments with the extracts showed viability higher than 8.59 log CFU/mL, as recommended for probiotic food products. The addition of green coffee bean, coffee silverskin, and PVA extracts did not reduce the encapsulation efficiency of the alginate microcapsules, enabling a safe interaction between the extracts and the K. lactis cells.

The Potential of Bacillus Species as Probiotics in the Food Industry: A Review.

The demand for probiotics is increasing, providing opportunities for food and beverage products to incorporate and market these foods as a source of additional benefits. The most commonly used probiotics belong to the genera of Lactobacillus and Bifidobacterium, and traditionally these bacteria have been incorporated into dairy products, where they have a wider history and can readily survive. More recently, there has been a desire to incorporate probiotics into various food products, including baked goods. In recent years, interest in the use of Bacillus species as probiotics has greatly increased. The spores of various Bacillus species such as Bacillus coagulans and Bacillus subtilis, have significantly improved viability and stability under harsher conditions during heat processing. These characteristics make them very valuable as probiotics. In this review, factors that could affect the stability of Bacillus probiotics in food products are highlighted. Additionally, this review features the existing research and food products that use Bacillus probiotics, as well as future research opportunities.

The Effect of Different Temperatures on Addition of Powdered Lactobacillus plantarum Dad-13 on Peanut Chocolate Jam Towards Total Lactic Acid Bacteria (LAB), Sublethal Injury, pH, and Total Acids

Lactobacillus plantarum Dad-13 is an indigenous probiotic that was isolated from Dadih. Peanut chocolate jam has the potential to be developed into a probiotic food product. However, peanut chocolate jam involves a heating process which can affect the number of probiotics and their metabolic processes. This research aims to determine the optimal temperature of L. plantarum Dad-13 probiotic powder in addition to peanut chocolate jam products. The different temperatures of probiotic powder L. plantarum Dad-13 addition at 30 oC, 40 oC, 50 oC, and 60 oC were used to measure total lactic acid bacteria (LAB), sublethal injury, pH value, and total acids. Total lactic acid bacteria and sublethal injury were measured by Total Plate Count (TPC), pH value was measured by pH meter and total acids were analyzed using the titration method. Results show significant differences (p<0,05) on total lactic acid bacteria (LAB) and sublethal injury. Total lactic acid bacteria (LAB) resulting in a value of 8.04±0.02 to 7.67±0.05 and sublethal injury 0.04±0.01 to 0.26±0.03. Meanwhile, no significant differences (p>0,05) in pH value (7.20±0.16 to 7.30±0.15) and total acids (0.09±0.01 to 0.08±0.01). The best temperature of L. plantarum Dad-13 probiotic powder addition on peanut chocolate jam was at 30–40 oC because it showed the highest amount of total lactic acid bacteria (LAB) and the least sublethal injury.

Bigel Matrix Loaded with Probiotic Bacteria and Prebiotic Dietary Fibers from Berry Pomace Suitable for the Development of Probiotic Butter Spread Product.

This study presents a novel approach to developing a probiotic butter spread product. We evaluated the prebiotic activity of soluble dietary fibers extracted from cranberry and sea buckthorn berry pomace with different probiotic strains (Limosilactobacillus reuteri, Lacticaseibacillus paracasei, and Lactiplantibacillus plantarum), uploaded selected compatible combination in the bigel matrix, and applied it in the probiotic butter spread formulation. Bigels and products were characterized by physical stability, rheological, textural properties, and viability of probiotics during storage at different conditions. The highest prebiotic activity score was observed in soluble cranberry (1.214 ± 0.029) and sea buckthorn (1.035 ± 0.009) fibers when cultivated with L. reuteri. The bigels loaded with probiotics and prebiotic fiber exhibited a significant increase in viscosity (higher consistency coefficient 40-45 Pa·sn) and better probiotic viability (>6 log CFU/g) during long-term storage at +4 °C temperature, surpassing the bigels loaded with probiotics alone. Bigels stored at a lower temperature (-18 °C) maintained high bacterial viability (above 8.5 log CFU/g). The butter spread enriched with the bigel matrix was softer (7.6-14.2 N), indicating improved spreadability. The butter spread product consistently met the required 6 log CFU/g for a functional probiotic food product until 60 days of storage at +4 °C temperature. The butter stored at -18 °C remained probiotic throughout the entire storage period, confirming the protective effect of the bigel matrix. The study's results showed the potential of the bigel to co-encapsulate, protect, and deliver probiotics during prolonged storage under different conditions.

Encapsulation techniques, action mechanisms, and evaluation models of probiotics: Recent advances and future prospects

AbstractThe related studies of probiotics‐based functional foods have recently attracted increasing interests in developing new types of oral administration systems. Probiotics has various biological functions, including increasing adsorption ability of nutrients, competitively rejecting pathogenic organisms, and regulating the immune system. It is well known that probiotics cannot be worked until their colonies reach the intestine alive, as well as the amount reaches 106–107 colony‐forming units/g at the end of product's shelf life. However, the delivery of probiotics to the colon through oral administration is always challenging due to weak viability under exposure to harsh conditions in the presence of ions and small molecules from the upper gastrointestinal tract. Moreover, the low viability of the probiotics due to the lack of resistance to the acidity of food matrices, high temperature during food processing, and oxygen changes during storage also limit the applications of probiotics in food products. Therefore, encapsulation of probiotics could particularly protect the probiotics from degradation and inactivation, resulting in enhanced viability during the transition from consumption to digestion in the gut. Emulsion cross‐linking, complex coacervation, microcapsules, spray drying, layer‐by‐layer self‐assembly, electrospinning, hydrogel, and other methods are used to encapsulate probiotics. Herein, this review mainly highlights the gut health functions and encapsulation techniques of probiotics, followed by the current challenges and future development prospects.

Formulation of a probiotic product using Almond gum.

The application of probiotics in food has expanded significantly, yet its viability remains a challenge. In response to this issue, this study explores a unique approach. Almond gum, a natural extract from Prunus dulcis, is utilized as the primary carrier matrix for a novel probiotic product featuring Saccharomyces boulardii, a probiotic yeast. This study involves the entrapment of S. boulardii in almond gum through centrifugation (5 min at 1300 × g) and subsequent 24 h drying at 50 °C. Sensory evaluation and other investigations were conducted at different pH levels to assess viability and performance. Post-drying entrapment efficiency was 83.85%, underscoring the benefits of choosing almond gum as a carrier matrix. Promising results were observed from viability testing conducted in gastric juice (pH 1.2) and in simulated intestinal fluid (pH 6.8). Matrix stability was assessed by measuring cfu ml-1 following 7 days' storage at different temperatures, complemented by sensory analysis. Almond gum is a promising carrier matrix for probiotic products. Its high entrapment efficiency and its viability under challenging pH conditions demonstrate its efficacy. It is rich in carbohydrates and serves a dual purpose by acting as a prebiotic source, as confirmed through ultraviolet-visible (UV-visible) analysis. The study underscores the potential of this novel approach, providing insights into responses to viability challenges in probiotic food products. © 2024 Society of Chemical Industry.

Selection of a Probiotic for Its Potential for Developing a Synbiotic Peach and Grape Juice.

Due to recent interest in the potential of probiotics as health promoters and the impact of health and environmental concerns on eating habits, non-dairy probiotic food products are required. This study aimed to evaluate the viability of different probiotic microorganisms in peach and grape juice (PGJ) with or without the prebiotic inulin and their antimicrobial activity against the foodborne pathogen Listeria monocytogenes and the juice spoilage microorganism Saccharomyces cerevisiae. Firstly, the viability of seven probiotic strains was studied in PGJ with an initial concentration of 107 CFU/mL for 21 days at 4 °C and for 3 days at 37 °C. In parallel, the physicochemical effect, the antimicrobial effect and the lactic acid production in PGJ were evaluated. Secondly, the probiotic with the best viability results was selected to study its antimicrobial effect against L. monocytogenes and S. cerevisiae, as well as ethanol and acetaldehyde production by the latter. L. casei showed the highest viability and grew in both refrigerated and fermentation conditions (1 log), produced the greatest lactic acid (5.12 g/L) and demonstrated in vitro anti-Listeria activity. Although the addition of the prebiotic did not improve the viability, lactic acid production or anti-Listeria activity of the probiotics, under the conditions studied, the prebiotic potential of inulin, support the design of a synbiotic juice. Finally, although none of the probiotic, fermentation products, or postbiotics showed any antimicrobial activity against L. monocytogenes or S. cerevisiae, the addition of L. casei to the PGJ significantly reduced the production of S. cerevisiae metabolite ethanol (29%) and acetaldehyde (50%). L. casei might be a suitable probiotic to deliver a safe and functional PGJ, although further research should be carried out to determine the effect of the probiotic and fermentation on the nutritional profile of PGJ.

КОНТРОЛЬ ВЫЖИВАЕМОСТИ БИФИДОБАКТЕРИЙ В НОВЫХ ВИДАХ ПРОБИОТИЧЕСКИХ ПИЩЕВЫХ ПРОДУКТОВ НА ФЕРМЕНТИРОВАННОЙ РАСТИТЕЛЬНОЙ ОСНОВЕ

This study aimed to determine the survival rate of bifidobacteria in new types of plant-based probiotic food products over their shelf life and to identify the identified cultures using polymerase chain reaction (PCR). A variety of new plant-based probiotic food products, including yogurt alternatives made from soy, almond, and coconut, and fermented plant-based beverages, were selected for the study. Microbiological indicators of product safety, such as total viable count, coliforms, and pathogens, were analyzed according to standard methods. The survival of bifidobacteria was evaluated throughout the shelf life of the products, as declared by the manufacturer, by plating on selective media and counting viable bifidobacteria colonies. Additionally, PCR analysis was performed to confirm the presence and identify the specific strains of bifidobacteria present in the samples. The microbiological analysis of the plant-based probiotic food products confirmed that they met the safety criteria, with no coliforms or pathogens detected. The survival of bifidobacteria was found to be variable among different products, but overall, bifidobacteria were able to maintain their viability throughout the entire shelf life stated by the manufacturer. PCR analysis confirmed the presence of live bifidobacteria in all samples and identified the specific strains present, including Bifidobacterium animalis subsp. lactis, Bifidobacterium bifidum, and Bifidobacterium longum. The results of this study demonstrate that new types of plant-based probiotic food products containing bifidobacteria are safe to consume and can provide a source of live beneficial bacteria throughout their shelf life.

Верификация оптимальных условий и параметров биотрансформации мышечной ткани филе рыб бактериальными заквасочными культурами

The article describes the relevance and expediency of using a method of biotechnological processing, namely biotransformation of fish raw materials by bacterial starter cultures, which is a priority of developing areas according to the Strategy of improving the quality of food products in the Russian Federation until 2030. The studied method of processing will positively affect the shelf life of fish products, its organoleptic properties and nutritional value due to the formation of metabolites of the vital activity of bacterial starter cultures, which are the main factor of bioconservation, synthesis of vitamins and other biologically active substances. Probiotic properties of bacterial starter cultures will have a significant impact on the optimization of the internal microflora of the gastrointestinal tract, thereby strengthening the human body and its immune system as a whole, which supports the principles of a healthy lifestyle. In accordance with this, studies have been conducted to study the possibility of using bacterial starter cultures for the biotransformation of fish raw materials in order to further obtain a probiotic food product based on it. Data on the effect of bacterial starter cultures on organoleptic microstructural, physico-chemical and functional-technological characteristics of fish raw materials are presented. The number of residual cells of bacterial starter cultures in the muscle tissue of fish fillets, which meets the requirements for probiotic products, was revealed. The survival rate under the influence of temperatures and the dynamics of changes in the number of bacterial starter cultures during storage in fish fillet muscle tissue samples were determined, the data on which will be used to develop and adjust the thermal treatment modes of the final product. The influence of bacterial starter cultures on the changes in total viable count after biotransformation and during storage is analyzed, on the basis of which the effect of bioconservation is justified. The results of the conducted studies became the basis for the selection of the most suitable bacterial starter cultures, as well as verification of optimal conditions and parameters predicted by mathematical modeling algorithms for biotransfor-mation of the muscle tissue of fish fillets. At the next stage, research will be conducted to study the possibility of using fish raw materials treated with bacterial starter cultures in the formulations of “fast food” products containing functional components, which is justified by the accelerated rhythm of life and the desire of modern society to monitor their nutrition by eating foods that correspond to a healthy lifestyle.

Consumers’ perceptions about probiotic food products and their effects on purchase intention: A case study of Eastern Mediterranean Region of Turkey

Consumers’ perceptions about probiotic food products and their effects on purchase intention: A case study of Eastern Mediterranean Region of Turkey

Evaluation of probiotic, safety, and anti-pathogenic properties of Levilactobacillus brevis HL6, and its potential application as bio-preservatives in peach juice

The inclusion of probiotics in food products is increasingly common as a method to develop functional foods that provide potential health advantages. This study examined the probiotic characteristics of Levilactobacillus brevis HL6, and its potential use in peach juice. The strain demonstrated resilience to low pH levels, bile salts, and gastrointestinal conditions. Its cell surface hydrophobicity (48.53%), auto-aggregation (35.20%), co-aggregation (23.10%), adhesion capacity (11.10%), antioxidant activity (46.80%), and cholesterol removal ability (41.50%) were remarkable. Staphylococcus aureus was most sensitive to Lev. brevis cell-free supernatants, while Escherichia coli was most resistant. The strain demonstrated no hemolytic or DNase activity and did not possess the ability to produce biogenic amines. Additionally, it exhibited susceptibility to nearly all antibiotics tested. Hence, this strain was incorporated into the peach juice model as a probiotic component at concentrations of 0, 0.1, and 0.2 g.L−1 During storage at 4 °C, the pH and acidity levels of the probiotic peach juices remained stable. The peach juice containing 0.2 g.L−1 probiotic had the highest viable cells (8.01 log CFU/mL) and overall acceptance compared to other samples. Peach juice could potentially serve as a suitable medium for Lev. brevis, paving the way for the formation of a health-promoting beverage.

Probiotic‐edible film in active packaging with antimicrobial effect and storage viability: a review

SummaryThe drive towards the consumption of healthy foods and adoption of beneficial eating habits has spurred to develop innovative probiotic food products with antibacterial characteristics. In recent years, researchers have been exploring the use of probiotic edible films with antimicrobial properties to improve the viability and effectiveness of probiotics in meals and to protect from harmful pathogens. However, there are various factors that can affect the effectiveness of probiotic edible films. These include the composition of the film, the type of probiotic used, and the conditions in which the film was stored and transported. This review provides a brief overview on the use of probiotic edible films in active packaging with antimicrobial effects, its storage stability, and future prospects. In summary, food system sustainability is crucial, and tackling packaging issues is vital. Synthetic plastic packaging harms the environment, so we need eco‐friendly alternatives like probiotic edible films. These films offer benefits like better food safety, longer shelf life, and pathogen protection. Challenges include preserving probiotics, dealing with regulations, and expanding use. Collaboration between science and the food industry is essential for a greener, healthier food future.

A Model for Probiotic Fermented Food Production

The past few decades have demonstrated how important the human gut microbiota is for human health. Because of this, the use of microbiota-modulating dietary interventions such as probiotics and prebiotics is growing in popularity amongst consumers, food manufacturers, healthcare professionals, and regulators. In particular, there is interest in making a wider variety of foods with probiotic properties. However, as a solution for food manufacturers to produce fermented foods compatible with the “probiotic foods” label definition, we used an impedometric analysis to identify the survival and growth capacity of microbial strains in specific environmental contexts. Using this approach, manufacturers can more effectively select the strains with the highest growth rate for use in probiotic fermented food production trials. To provide a proof of concept, we tested three Lacticaseibacillus rhamnosus probiotic strains growing in milk at different temperatures. We quantified the probiotic’s growth using species-specific primers and quantitative real-time PCR. Overall, our results demonstrate the feasibility of this type of model in facilitating the production of probiotic fermented foods by allowing manufacturers to select strains able to grow under specific conditions. Our model can be used to develop, increase, and target the beneficial health properties of a multitude of fermented foods produced worldwide.

Production and Characterization of a Novel Symbiotic Plant-based Beverage Rich in Antioxidant Phenolic: Mung Bean and Rye Sprouts.

There is an increasing demand for non-dairy probiotic food due to the constraints associated with dairy probiotics. In this study, a co-culture synbiotic beverage was prepared using a mixture of mung bean and rye sprouts inoculated with Lactobacillus plantarum (B-28) and Lactobacillus casei (B-29), along with inulin and oligofructose as prebiotics. The effects of prebiotic addition and starter culture on the survival of probiotics during cold storage and simulated gastric conditions were examined. Additionally, titratable acidity, pH, phenolic content, antioxidant activity, and sensory characteristics were evaluated over a 28-day period. The resulting product demonstrated good survival for L. casei (107CFU.ml-1) and L. plantarum (106CFU.ml-1) after 4weeks under refrigeration with no significant changes in quality. The samples exhibited significantly high total phenolic content (TPC), ranging from 19.18 to 25.75mg GAE/100mL, which L. casei-containing drinks exhibited the highest TPC activity (p < 0.05). All treatments showed a significant reduction in probiotic survival during gastrointestinal digestion in the laboratory conditions (p < 0.05), although more than 50% survival was observed for all strains. The addition of prebiotics to the beverages led to a significant decrease in phenolic content (p < 0.05), but improved sensory scores. The highest turbidity was observed in the sample containing both probiotics and inulin on the 28th day at 38.1 (NTU). In general, the synergistic effect of probiotics was more pronounced when used together with both prebiotics in the beverages compared to their individual use. The results suggest that the production of this beverage could serve as a nutritious alternative to lactose-sensitive dairy beverages and contribute to the development of future probiotic food products.

Online Monitoring of the Growth of Probiotic Bacteria and Metabolites in the Fermentation of a Teff Substrate Using Model-Based Calibration of 2D Fluorescence Spectra.

The demand for probiotic bacteria-fermented food products is increasing; however, the monitoring of the fermentation process is still challenging when using conventional approaches. A classical approach requires a large amount of offline data to calibrate a chemometric model using fluorescence spectra. Fluorescence spectra provide a wide range of online information during the process of cultivation, but they require a large amount of offline data (which involves laborious work) for the calibration procedure when using a classical approach. In this study, an alternative model-based calibration approach was used to predict biomass (the growth of Lactiplantibacillus plantarum A6 (LPA6) and Lacticaseibacillus rhamnosus GG (LCGG)), glucose, and lactic acid during the fermentation process of a teff-based substrate inoculated with mixed strains of LPA6 and LCGG. A classical approach was also applied and compared to the model-based calibration approach. In the model-based calibration approach, two-dimensional (2D) fluorescence spectra and offline substituted simulated data were used to generate a chemometric model. The optimum microbial specific growth rate and chemometric model parameters were obtained simultaneously using a particle swarm optimization algorithm. The prediction errors for biomass, glucose, and lactic acid concentrations were measured between 6.1 and 10.5%; the minimum error value was related to the prediction of biomass and the maximum one was related to the prediction of glucose using the model-based calibration approach. The model-based calibration approach and the classical approach showed similar results. In conclusion, the findings showed that a model-based calibration approach could be used to monitor the process state variables (i.e., biomass, glucose, and lactic acid) online in the fermentation process of a teff-based substrate inoculated with mixed strains of LPA6 and LCGG. However, glucose prediction showed a high error value.

Quality Analysis of Probiotic Animal Food Products: A Test of Protein and Blood Fats

Dissemination on Bangka Island will be carried out at the UBB Probio_FM Technology Product Downstream Partnership activity for the Institutionalization of Community Enterprises based on Organic Agriculture.The dissemination program on Bangka Island is aimed at increasing the number of technology users on Bangka Island. Animal food products are one of the agricultural commodities that have high economic value, especially on Bangka Belitung Island. Animal food products are a source of protein, examples of animal food products include fish, chicken and duck meat, eggs, and beef. Catfish, tilapia, duck and broiler and laying duck farming businesses will be carried out in the MF activities of the 2022 design shop, with the aim of producing healthy food products. The results of farming activities such as fresh catfish, tilapia, eggs, chicken meat and ducks, will be used as further research samples to analyze the content of protein, fat and cholesterol. The purpose of this study is to analyze the content of cholesterol, protein, and blood fats in duck eggs, chicken and duck meat, and probiotic fish. Produce probiotic food products with labels containing cholesterol, protein, and fat values. The timing of the implementation of this research began in October and ended in December 2022. The research was conducted at the FPPB UBB Basic Laboratory. The form of research is laboratory research, using a random design method using several samples as a comparison of treatments, preparing samples, testing cholesterol and fat protein content, collecting data and analyzing data. The protein content obtained was duck 31.34%, duck eggs 1.229%, probiotic catfish 42.37%, and non-probiotic catfish 34.8%. The fat content obtained was duck meat 33.79%, duck eggs 46.21%, probiotic catfish 21.46%, and non-probiotic catfish 21.359%.&#x0D; Keywords: Animal Food Products, Probio FM Technology, Protein Content, Fat Content.

PROBIOTIC CHARACTERIZATION OF SACCHAROMYCES CEREVISIAE Y196 AND Y197 ISOLATED FROM RICE CHHANG- A FERMENTED BEVERAGE OF LAHAUL SPITI

Fermented foods and beverages are rich source of probiotic microorganisms and yeasts are the most common microorganisms found in these fermented products. Fermented beverages are less explored for probiotics, therefore; the present study was focused on isolation, screening and characterization of probiotic yeast from rice Chhang, a fermented beverage of Lahaul Spiti. Ten yeast isolates were initially isolated from rice Chhang and out of which two isolates Saccharomyces cerevisiae Y196 and Saccharomyces cerevisiae Y197 were selected based on their antimicrobial activity. These two yeast isolates were examined for various probiotic properties such as survival at low pH, bile tolerance, microbial adhesion to hydrocarbons, cholesterol assimilation, exopolysaccharide production and haemolytic activity. Both isolates showed antimicrobial activity against all the tested pathogens used in this study, also showed high acid tolerance at pH 2 and pH 3 and can withstand high bile concentration as compared to control. Percentage of hydrophobicity was found to be 57 % and 41 % in Saccharomyces cerevisiae Y196 and Saccharomyces cerevisiae Y197, respectively. Both isolates were able to show high cholesterol assimilation with a maximum of 90 % in case of Saccharomyces cerevisiae Y197 with taurocholate. They were also found to be positive for exopolysaccharide production and showed no haemolytic activity. Hence, keeping in view the results of present study, these isolates can be proposed for preparation of various probiotic food products.

Isolation and Characterization of Lactobacillus crispatus, Lactococcus lactis, and Carnobacterium divergens as Potential Probiotic Bacteria from Fermented Black and Green Olives (Olea europaea): An Exploratory Study.

Table olives are becoming well recognized as a source of probiotic bacteria that might be used to create a health-promoting fermented food product by traditional procedures based on the activities of indigenous microbial consortia present in local environments. Methodology. In the present study, the characterization of probiotic bacteria isolated from mince, chunks, and brine of fermented green and black olives (Olea europaea) was done based on morphological, biochemical, and physiological characteristics. Bacterial isolates demonstrated excellent survival abilities at 25, 37, and 45°C and at a variable range of pH. However, the optimum temperature is 37 and the optimum pH is 7 for all three isolates. An antimicrobial susceptibility pattern was found among these isolates through the disc diffusion method. Most of the isolates were susceptible to streptomycin, imipenem, and chloramphenicol, whereas, amoxicillin showed resistance to these isolates, and variable results were recorded for the rest of the antibiotics tested. The growth of the isolates was optimum with the supplementation of 3% NaCl and 0.3% bile salt. The isolated bacteria were able to ferment skimmed milk into yogurt, hence making it capable of producing organic acid. Isolates of Lactobacillus crispatus MB417, Lactococcus lactis MB418 from black olives, and Carnobacterium divergens MB421 from green olives were characterized as potential candidates for use as starter cultures to induce fermentation of other probiotic food products.

Probiotic microorganisms – Propionibacterium ssp

Propionic acid bacteria belong to probiotic microorganisms, probiotic properties of which have not yet been thoroughly investigated. The research describes the probiotic features of milk propionic acid bacteria, including growth at high acidity, tolerance to bile acid, ability to suppress pathogenic microorganisms, and antibiotic susceptibility, as well as the ability to extend the shelf life of various fermented milk products due to the synthesis of propionic acid. Propionic acid bacteria have a robust nature, which makes it easy to overcome technological difficulties in the process of optimizing production. This enables them to be used in the future for the production of various fermented probiotic foods.