Probiotic Complex Research Articles

The Suppressive Effect of Supplementation of Combined Probiotic on Helicobacter pylori Infection

Abstract Objectives Helicobacter pylori (H. pylori) is the leading cause of chronic gastritis and gastric ulcers and significantly increases the risk of developing gastric cancer. Even though many therapeutic improvements in H. pylori eradication rate, it is still associated with the high prevalence of infection. Several studies have verified that probiotics treatment were beneficial to gastrointestinal tract infections is increasingly documented as an alternative to antibiotics. This study aimed to investigate the efficacy of novelty probiotic complex on H. pylori infection in patients. Methods Between October 2018 and August 2019, all subjects with a clinical of Chung Shan Medical University Hospital in Taiwan, subjects who confirmed H. pylori infection (n = 20) were randomized to receive probiotic complex or placebo for 3 weeks. The probiotic complex contained Lactobacillus johnsonii No.1088 (HK-LJ88) 1010 CFU/mL, Bacillus subtilis 1012 CFU/mL, green tea extract and sake lees. Primary outcome of this study was The amounts of change of 13C urea breath test (13C UBT) that was assessed 3 weeks after the end of supplementation. The hematological results, dietary records, anthropogenic indicators were measured/assessed at the 0 and 3th week. Results Subjects in the probiotic group had significantly lower 13C UBT (39.6%) at the 3th week when compared to the value at week 0. In contrast with subjects in the placebo group had higher 50% 13C UBT. The levels of anthropometric indices, blood glucose and lipid profiles had no significant changes during the intervention study period in both groups. After adjusting for age and gender, supplement had significant effect on reducing the change of 13C UBT (β = -13.64, p = 0.047) and diastolic blood pressure (β = -6.91, p = 0.03). Conclusions These results suggest that supplement with the combination of probiotic Lactobacillus johnsonii and Bacillus subtilis for 3 weeks may have a favorable effect on H. pylori infection in humans. The probiotics intervention also resulted in improved blood pressure. Funding Sources Yang Ming Biomed Co., Ltd.

Synergism of components as the main approach to creation of a probiotic complex

Synergism of components as the main approach to creation of a probiotic complex

Influence of probiotic complex on productive performance and metabolism in growing pigs

Topicality. To date, an extensive scientific and practical material has been accumulated to study the effectiveness of probiotic feed additives in the feeding of farm animals. Some mechanisms of their action on the animal body have been studied, positive effects have been established, which are expressed in improving the microbial balance of the intestine, increasing the protective properties of the body, and realizing the productive qualities of animals. It is necessary to study the effectiveness of the use of probiotic complexes in comparison with the action of antibiotic-containing drugs.Methodology. It was studied the probiotic complex of Enzymsporin containing a consortium of bacteria of the genus Bacillus (Bacillus subtilis VKM B-2998 D, Bacillus licheniformis VKM B-2999 D, Bacillus subtilis VKM B-3057D) at least 5x109 CFU/g was studied in comparison with antibiotic-containing drug Virginiamycin in feeding fattened young pigs. The studies were carried out on four groups (30 heads in each group) of growing pigs in the conditions of LLC “Bryansk meat processing plant” and in the laboratories of L.K. Ernst Federal Science Center for Animal Husbandry. The duration of the experiment was 113 days.Results. During the studies, the control group of animals received compound feeds without probiotic additives, while the animals of the 2nd and 3rd experimental groups received compound feed with the addition of probiotic complex in an amount of 0.5 and 1.0 kg/t, the 4th experimental group of pigs received compound feed with the addition of antibiotic-containing drug Virginiamycin 250 g/t. By the end of the study, the live weight and average daily increments of experimental animals increased by 5.0; 6.0; 10.02 and 5.8; 6.5; 12.2% (p < 0.001), respectively, compared with the control. Biochemical, morphological, immunological blood indices corresponded to physiological norms, the inclusion in the feed of pigs fed different levels of probiotic complex has led to a significant increase lysozyme, bactericidal and phagocytic activity of blood in animals 2nd and 3rd groups during rearing. Additional profit for the period of research was in the experimental groups of fattened young pigs +705,0; +849,0 and 1440.0 rubles/ head, accordingly, in comparison with the control, which indicates the effective use of not only antibiotic-containing drugs in feeding pigs, but also suggests the effective use of probiotics instead of antibiotic-containing feed preparations.

The use of green mass of Jerusalem artichoke together with the “Enzimsporin” probiotic complex and the “Fungistat-GPK” sorbent as part of compound feeds for rabbits

To evaluate the effectiveness of the “Enzimsporin” probiotic preparation together with grass meal from Jerusalem artichoke green mass for feeding young rabbits, 200 French “Hypharm” rabbits (males) rabbits (males) were selected in the conditions of the “Lipetsk rabbit” LLC industrial complex, which are 45 days old on the principle groups – analogues were divided into 2 groups. Fattening was carried out until the age of 105 days. Rabbits of the 1st group (control) received mixed feed without additives, rabbits of the 2nd group were additionally injected with the probiotic preparation “Enzimsporin” 1.0 g/kg of mixed feed and a sorbent-toxin neutralizer “Fungistat-GPK” 2.0 g / kg mixed feed along with grass meal from green mass of Jerusalem artichoke in an optimally selected dosage of 15% by weight of the mixed feed. Upon reaching the age of 105 days, rabbits of the 1st group (control) were characterized by live weight, which was less than the weight of individuals of the 2nd group - by 284.0 g or 9.80%. The safety of the livestock in the control group was 90%, in the 2nd group 100%. An analysis of the morphological composition of rabbit carcasses showed that the use of the studied feed additives contributes to a more intensive growth of muscle tissue and an increase in the quality indicators and nutritional value of the obtained raw meat. Thus, the “Enzimsporin” preparation together with herbal flour from the green mass of Jerusalem artichoke is a promising product from a number of probiotics used in animal feeding and can increase the productivity and quality of rabbit meat.

Лечебно-профилактическая эффективность пробиотического комплекса при инфекциях, протекающих с сочетанным поражением дыхательной и пищеварительной систем

Лечебно-профилактическая эффективность пробиотического комплекса при инфекциях, протекающих с сочетанным поражением дыхательной и пищеварительной систем

Use of probiotic complexes in feed diets to improve the quality of rabbit meat

We examined the efficiency of probiotic “Sporotermin” and “A2” for increasing the productivity and physiological status of the rabbits. The studies were conducted on a crossbreed population of rabbits (Soviet chinchilla and New Zealand red) at the age of 45 days, divided into control and experimental groups of 15 heads in each by the method of pairs-analogues in the vivarium of the faculty of veterinary medicine and animal husbandry technology of the Voronezh State University in 2017-2018. Rabbits of group 1 (control) received all-mash without additives, rabbits of group 2 and 3 additionally received probiotic preparation “Sporotermin” in a dosage of 1.0 g/kg of feed (rabbits of group 2) and “A2” in a dosage of 0.6 g/kg of feed (rabbits of group 3). Upon reaching the age of 120 days, the live weight of all groups exceeded 3.0 kg, while rabbits of the 2nd group had an advantage over individuals of the 1st and 3rd groups by 295.0 g (9.10 %) and 115.0 g (3.36%). At the end of the experiment (120 days) in animals of the 2nd and 3rd groups there was a significant increase in the amount of total blood serum protein by 5.59 and 3.92% (P>0,95), respectively, compared with the control, as well as an increase in the amount of hemoglobin. In the experimental groups carcass yield made up 60.38 % and 56.88% (P>0,99), while in the control amounted to 55.56 % Thus, the preparation “Sporotermin” is a promising product from a number of probiotics used in animal nutrition and allows improving the productivity and meat quality of rabbits.

Probiotics and animal feed in primates feeding

This paper presents the results of experiments on primates feeding. In diet structures that were used animal feed, of which fish flour is the most valuable, as found, for monkeys, due to its unique chemical composition. And the effect of the probiotic complex “Bactistatin” on the digestibility of feed in the diet of rhesus monkeys was studied. This preparation belongs to the new generation of probiotics. It is a biological product with immobilized probiotic strains and their metabolites. The study was conducted on a large population of male rhesus macaques in enclosure conditions. Feeding diets, their nutritional value as a result of the inclusion of fish flour in the amount of 18% were studied. And also, the effect of “Bactistatin” in the amount of 3 g/head, on the formation of symbiotic microflora of the gastrointestinal tract, was studied in order to improve the absorption of nutrients in complete feed. Changes in homeostasis during the experiment were controlled by hematological and biochemical parameters of the blood of both experimental and control primates. At the end of the experiment, the cost of feed, nutrients, and exchange energy per head was calculated. The experimental data obtained were analyzed and mathematically processed.

Improving physiological status and meat quality of rabbits by the application of probiotic complex “Sporotermin” in compound feeds

The productive qualities of rabbits and meat quality depend on proper feeding. But due to the intensive technologies introduction in rabbit breeding, the animals stress sensitivity increases, their physiological status decreases, causing pathological conditions. Probiotic additives enhancing the animals immune status and resistance and having a positive effect on their productivity are introduced into the production cycle to increase the safety of young animals, as well as to restore and maintain the microflora of the digestive tract. This approach allows to reduce the consequences of the uncontrolled antibiotics use , which contribute to increased variability of pathogenic microflora and their accumulation in the breeding object tissues. The effectiveness of the Sporothermin probiotic complex application to increase the physiological status and efficiency of rearing young rabbits was studied in the work. The studies were carried out on a cross-breeding stock of rabbits obtained by industrial crossing of the parental forms of the breeds Soviet chinchilla and New Zealand red at the age of 60 days in the industrial complex LLC Lipetsk rabbit. The effect of the probiotic complex on the immune and clinical status was studied in 60 rabbits, divided into control and experimental groups using the pair-analogue method. Rabbits of the control group received PK-90 compound feed, the experimental group rabbits were fed with PK-90 compound feed with the introduction of the Sporotermin probiotic complex in a dosage of 0.6 and 1.0 g / kg of compound feed. The application of a probiotic complex in an optimally selected dosage of 1.0 g / kg of compound feed had a positive effect on the growth rate, physiological status of the organism and the quality indicators of rabbit meat, which is the basis for its use in industry

Use of different concentrations of enzymesporin probiotic in feeding of growing young pigs

The study, which included four groups of growing young pigs (each consisting of 30 animals), was arranged under conditions of the Bryansk meat-processing plant and laboratories of the Ernst Federal Research Center of Animal Husbandry. The duration of the experiment was 113 days. Animals of the group 1 (control) were fed with the SK-4, SK-5 and SK-6 mixed fodders without any probiotic additives. The Groups 2 and 3 were fed by a mixed fodder supplemented with the Enzymesporin probiotic (0.5 and 1.0 kg t-1, respectively). The Group 4 was fed by a mixed fodder supplemented with the Virginiamycin antibiotic (250 g t-1). The feeding of different concentrations of the Enzymesporin probiotic complex and Virginiamycin antibiotic to the growing young pigs of the experimental Groups 2, 3, and 4 provided the average daily liveweight gain equal to 771.4, 775.4, and 818.8 g (5.8, 6.5, and 12.2%), respectively, as compared to the control. Addition of different dosages of the probiotic complex or the antibiotic to the fodder fed to the growing young pigs (Groups 2, 3, and 4) did not result in a significant increase in the lysozyme and bactericidal activity of blood serum and the phagocytic activity, though a tendency to the phagocytic index increase was observed. Addition of the probiotic complex to forage did not provide any negative impact on the intestinal microbiocoenosis; moreover, it positively influenced on the growth of lactobacteria.

Influence of the efficacy of a probiotic complex containing Bacillus subtilis and Pichia farinosa on the growth performance and fecal microbiota of finishing pigs

A total of 120 finish pigs [(Landrace × Yorkshire) × Duroc; 52.61 ± 2.37 kg] were randomly allotted into three treatments for a 70 d trial to evaluate the growth performance and fecal microbiota counts in finishing pigs. Treatment groups were (i) basal diet (CON), (ii) CON + 0.1% probiotic complex (TRT1), and (iii) CON + 0.2% probiotic complex (TRT2). Each treatment consisted of eight replicate pens with five pigs per pen. The average daily gain and fecal Lactobacillus counts of TRT2 were increased (P < 0.05) compared with CON treatment. The result showed that 0.2% complex probiotic was more effective than 0.1%.

Comparative effects of low zinc oxide dose with or without probiotics relative to high zinc oxide dose on the performance, nutrient digestibility, blood metabolites, and noxious gases emission in weaned piglets

The objective of this study was to assess the effects of low-dose zinc oxide (ZnO) supplemented with or without probiotic complex compared with pharmacological ZnO (3000 mg kg−1) on the performance, digestibility, blood metabolites, fecal Lactobacillus and enterobacteria counts of weaned piglets. One-hundred and twenty crossbred piglets were randomly allocated to three treatments based on their initial body weight (BW). Treatments consisted of corn–soybean-meal-based basal diet supplemented with 3000 mg kg−1 ZnO as positive control (ZH), basal diet supplemented with 300 mg kg−1 ZnO as negative control (ZL), and ZL + 0.1% probiotic complex (ZLP). At the end of the experiment, fecal samples were collected by direct rectal massage to determine nutrient digestibility, Lactobacillus and enterobacteria counts, and gas emission, whereas blood samples were taken via jugular venipuncture for determination of blood metabolites. The BW of piglets at week 6, and the average daily gain (ADG) at week 6 and during overall period were higher (P < 0.05) in pigs fed ZH diet compared with those fed ZL diet. The supplementation of ZLP increased the ADG during week 6 and overall making it comparable with ZH diet (P < 0.05). However, other parameters described above were comparable with ZH in pigs fed ZLP diet.

The impact of compound feeds with probiotic complexes on metabolism and productive indicators of rabbits

Probiotic preparations based on bacteria of different species composition, having the ability to increase the resistance and physiological status of the organism of the breeding object, are used to increase the productivity and preservation of the livestock of bred animals under the conditions of recent intensive cultivation technologies. The effectiveness of the probiotic complex A2 using to increase the productivity and quality of rabbit meat was studied by us. The studies were carried out in LLC "Lipetsk rabbit' on a hybrid livestock "Hypharm" of French selection at the age of 45 days. The experimental population was divided into control and experimental groups by the method of pair-analogues. The rabbits of the control group received PZK-92 compound feed, the livestock of the experimental groups was fed with PZK-92 compound feed with the introduction of the A2 probiotic complex in dosages of 0.6 and 1.0 g / kg of compound feed. When applying the optimal dosage of the used probiotic complex, which amounted to 0.6 g / kg of feed, 100% livestock safety is achieved, slaughter yield is increased by 4.51% with a decrease in feed costs by 1.42 ECE, as well as a positive effect on nutritional and biological value of rabbit meat was observed. An increase in profit by 3616.0 and profitability (up to 35%) by 12% in relation to the control group (23%) was achieved. This creates the prerequisites for the widespread use of this feed additive in the industrial complexes of the rabbit industry.

Improvement of rabbit productivity using probiotics and herbal supplements

The use of probiotic supplements that normalize the microbiocenosis and enhance the resistance to pathogenic microflora can increase safety and meat productivity of farm animals. The aim of the article is scientific and practical substantiation of the increase in rabbit productivity using girasole beet pulp and the “Sporothermin” probiotic complex. The effects of the “Sporothermin” girasole beet pulp on safety, productivity, physiological status and chemical composition of rabbit meat were studied. 30 cross-bred rabbits aged 45 days were selected. The control group received the basic ration consisting of the PK-90 feed; the experimental groups received the PK-90 feed which included the probiotic complex “Sporothermin” (0.6 g/kg and 1.0 g/kg) and girasole beet pulp (10% to the feed weight). The quality of meat was evaluated at the age of 120 days after the control slaughter in the amount of 3 heads from each group. The data of morphological composition showed that rabbits of the experimental groups had a larger muscle tissue mass than rabbits of the control group by 9.07% and 20.15%, respectively. The analysis of the muscle tissue composition showed that the use of the Sporothermin at a dosage of 1.0 g/kg and girasole beet pulp at a dosage of 10% to the feed weight weight increases the average daily gains and meat productivity, improves the chemical composition which was confirmed by testing the data in the conditions of the industrial company “Lipetsk rabbit” and assessing physicochemical, functional, technological and organoleptic indicators of the resulting meat raw materials.

The effect of probiotics on the secretory immunity of saliva in patients with type 2 diabetes

To study the effect of the use of probiotics on the state of mucosal immunity in patients with type 2 diabetes. Evaluation of the results was carried out based on immunological parameters of the saliva of 50 patients suffering from type 2 diabetes with moderate periodontitis, before and 1 month after taking a probiotic complex based on Lactobacillus. In the salivary fluid of patients, a decrease in the concentration of pro-inflammatory cytokines IL-1β, TNF-α and IL-8 was noted, while an increase in the anti-inflammatory cytokine IL-10 was observed. An increase in local mucosal immunity was also observed, manifested in an increase in the synthesis of secretory immunoglobulin A.

Evaluation of an Orally Administered Multistrain Probiotic Supplement in Reducing Recurrences Rate of Bacterial Vaginosis: A Clinical and Microbiological Study

Background: Bacterial vaginosis (BV) is the most common urogenital disease in women, affecting about 19% - 24% of them in reproductive ages annually and after treatment, a single recurrence or more may occur in up to 58% of women within 12 months. Objective: The aim was to evaluate the effectiveness of a new orally administered food supplement, containing different probiotic strains, on women of childbearing age after the antibiotic treatment when compared with no probiotic intake. Methods: A prospective study was undertaken on 62 patients with BV. All patients were cured with metronidazole vaginal formulations (5 g of 0.75% gel once daily for 5 days or 500 mg ovules once daily for 7 days), then after was offered the option of using a new an orally administered food supplement containing: Lactobacillus plantarum PBS067, Lactobacillus rhamnosus LRH020 and Bifidobacterium animalis lactis BL050, with a total viability of 3 × 109 CFU/capsule (Intimique® Femme). Among these women, 50 accepted to use the new orally food supplement, while 25 patients decided to use only metronidazole (control group). Results: The recurrence rate of BV after treatment with Intimique® Femme was about 16%, compared to 40% in the control group. The incidence of abnormal vaginal microbiota decreased in both groups, but it was significantly higher in the Intimique® Femme group at the end of treatment. Conclusion: This study showed that, in case of BV diagnosis, the complementary treatment of a strain-specific probiotic complex after antibiotics prophylaxis, is mandatory to reduce potential recurrences and cyclic use of further antibiotics.

PSIX-17 Effects of dietary Allium mongolicum Regel leaves or probiotic complexes on mutton eating quality and antioxidant activities of DuHan sheep.

PSIX-17 Effects of dietary Allium mongolicum Regel leaves or probiotic complexes on mutton eating quality and antioxidant activities of DuHan sheep.

Growth Performance and Post-Weaning Diarrhea in Piglets Fed a Diet Supplemented with Probiotic Complexes.

Weaning stress can affect the growth performance and intestinal health of piglets. Dietary alternatives to antibiotics, such as dietary probiotics, especially those containing multiple microbial species, are a preventive strategy for effectively controlling post-weaning diarrhea. In this study, we investigated forty-eight crossbred piglets in three treatment groups for 21 days: the control and experimental groups were supplemented with Enterococcus faecium DSM 7134, Bacillus subtilis AS1.836 plus Saccharomyces cerevisiae ATCC 28338 (EBS) or Lactobacillus paracasei L9 CGMCC No. 9800 (EBL). On day 21, weaned piglets supplemented with two kinds of probiotic complexes showed increased growth performance and significantly reduced post-weaning diarrhea (p ＜ 0.05). The EBS treatment increased acetic acid and propionic acid in the feces (p ＜ 0.05), and the EBL treatment increased fecal acetic acid, propionic acid, butyrate and valerate (p ＜ 0.05). Moreover, the fecal microbiota of the piglets changed markedly in EBL treatment. The addition of EBS and EBL may have similar effects on the prevention of diarrhea by improving the intestinal morphology and regulating the microbiota during the weaning period.

A probiotic complex, rosavin, zinc, and prebiotics ameliorate intestinal inflammation in an acute colitis mouse model

BackgroundAn altered gut microbiota balance is involved in the pathogenesis of inflammatory bowel disease (IBD), and several probiotic strains are used as dietary supplements to improve intestinal health. We evaluated the therapeutic effect of 12 probiotics in combination with prebiotics, rosavin, and zinc in the dextran sodium sulfate (DSS)-induced colitis mouse model.MethodsThe probiotic complex or the combination drug was administered orally to mice with DSS-induced colitis, and the body weight, disease activity index, colon length, and histopathological parameters were evaluated. Also, the combination drug was applied to HT-29 epithelial cells, and the expression of monocyte chemoattractant protein 1 (MCP-1) was evaluated by real-time polymerase chain reaction.ResultsAdministration of the combination drug attenuated the severity of DSS-induced colitis. Moreover, the combination drug significantly reduced the levels of the proinflammatory cytokines tumor necrosis factor-α, interleukin (IL)-6, IL-1β, and IL-17, and significantly increased the levels of Foxp3 and IL-10 in colon sections. Additionally, treatment with the combination drug reduced MCP-1 expression in HT-29 cells. Treatment with the combination drug decreased the levels of α-smooth muscle actin and type I collagen compared with vehicle treatment in mice with DSS-induced colitis.ConclusionThese results suggest that the combination of a probiotic complex with rosavin, zinc, and prebiotics exerts a therapeutic effect on IBD by modulating production of pro- and anti-inflammatory cytokines and the development of fibrosis.

The Combination of Probiotic Complex, Rosavin, and Zinc Improves Pain and Cartilage Destruction in an Osteoarthritis Rat Model.

Osteoarthritis (OA), a degenerative disorder, induces pain, joint inflammation, and destruction of the articular cartilage matrix. Probiotic complex, rosavin, and zinc have been used as dietary supplements that exhibit anti-inflammatory and antioxidant properties. However, there is no evidence demonstrating a synergic effect in OA. This study aims to determine whether combination with probiotic complex, rosavin, and zinc decreases progression of monosodium iodoacetate (MIA)-induced OA rat model. The combination improved pain levels by preventing cartilage damage. The expression of proinflammatory cytokines and catabolic factors was reduced by the combination within the joint tissue. However, the combination increased anti-inflammatory cytokines as well as the anabolic factor production. The gene level of catabolic factors was decreased with treatment of the combination in chondrocytes isolated from OA patients. These results suggest that the combination can improve MIA development through the inhibition of proinflammatory cytokines and cartilage destruction, thus playing a key role as a therapeutic candidate for OA treatment.

Пробіотичні комплекси TM GOODFOOD як ефективний підхід до корекції порушення харчування у дітей раннього віку

Пробіотичні комплекси TM GOODFOOD як ефективний підхід до корекції порушення харчування у дітей раннього віку