Emissive Probe Research Articles

An Open-Source Smartphone Otoacoustic Emissions Test for Infants.

Universal hearing screening is essential for early identification of infants with hearing loss, yet there is a lack of low-cost, scalable equipment suitable for resource-constrained settings. Here we test a low-cost smartphone device for infant hearing screening. Infants aged 0 to 6months were recruited from 3 ambulatory clinics at Seattle Children's Hospital with a high prevalence of hearing loss. We compared results from a low-cost open-source distortion product otoacoustic emission (OAE) probe and smartphone app with results from a commercially available OAE device. Hearing status was confirmed using newborn hearing screening, diagnostic testing, or both. Primary outcomes were referral rate as well as sensitivity, specificity, positive predictive value, and negative predictive value compared with known hearing status. Among N = 76 infants, the mean age at screening was 3.1 ± 1.9months and 13% had hearing loss. Referral rates were 24% and 26% for the smartphone and conventional devices, respectively. Both devices demonstrated 100% sensitivity (95% CI, 0.69-1.00) and negative predictive value (95% CI, 0.94-1.00). Specificity was 88% (95% CI, 0.78-0.95) and 85% (95% CI, 0.74-0.92) for the smartphone and conventional devices, respectively. Positive predictive value was 56% (95% CI, 0.31-0.78) for the smartphone and 50% (95% CI, 0.27-0.73) for the conventional device. The smartphone-based OAE device effectively screened hearing in high-prevalence infants. Thus, smartphone-based OAE detection may be a promising low-cost solution to the challenge of building scalable universal newborn and infant hearing screening programs in resource-constrained settings.

Spatial Resolution Measurement of Microviscosity Using Length-Regulated Aggregation-Induced Emission Probes.

The measurement of microviscosity gradients across different regions of self-assembled systems is crucial for optimizing their performance in both biological and industrial applications. However, this task has long been challenging due to the spatial barrier within the self-assembled systems, the interference from polarity gradients, and the lack of region-positioned probes. To overcome these challenges, we developed three kinds of length-regulated aggregation-induced emission (AIE) probes for the spatial resolution measurement of microviscosity. These AIE probes with varying alkyl chain lengths showed high sensitivity (0.81) over a viscosity range from 2 to 435 mPa s and unaffected luminescence to environmental polarity, ensuring accurate measurements in diverse environments. Using micelles as a model of self-assembled systems, these AIE probes were able to localize selectively in different regions ranging from the hydrophobic core to the hydrophilic shell and interface. The results indicated that microviscosity was highest in the core and gradually decreased toward the outer regions. Furthermore, these AIE probes were successfully applied for monitoring microviscosity in hydrogels and food thickeners, showing a strong correlation among fluorescence intensity, tensile strength, and thickening effects. These findings underscore the potential of length-regulated AIE probes for evaluating the microviscosity in diverse applications.

NIR emissive probe for fluorescence turn-on based dead cell sorting and in vivo viscosity mapping in C. elegans.

Dead cell sorting is pivotal and plays a very significant role in homeostasis. Apoptosis and ferroptosis are the two major regulatory cell death processes. Apoptosis is a programmed cell death process, while ferroptosis is a regulatory cell death process. Monitoring the dead cells coming out from these processes is extremely important to stop various cellular dysfunctions. Here, we present a single NIR emissive probe that can observe both apoptotic and ferroptosis regulatory cell deaths. We were able to directly visualize the dead cells in both animal and plant cells upon a significant increase in the fluorescence intensity of the probe. During cell death, the increased cytoplasm viscosity restricted the rotor motion and helped in the fluorescence turn-on of the probe. Lysosomal viscosity was found to play a crucial role in the ferroptosis pathway. On the other hand, the probe was not only efficient in mapping the viscosity in various parts of live Caenorhabditis elegans (C. elegans) bodies but also able to differentiate between live and dead animals.

Probing Cell Membrane Tension Using DNA Framework-Encoded Vibration-Induced Emission Molecular Assemblies.

Mechanosensitive fluorescent probes are valuable tools for detecting changes in cellular mechanics and viscosity. While numerous mechanosensitive probes have been developed, the construction of molecular assemblies for probing cellular mechanics remains largely unexplored, possibly due to the challenges of designing assemblies with synergistic and integrated functionalities. Here, we report the design and synthesis of mechanosensitive molecular assemblies by integrating DNA frameworks with vibration-induced emission (VIE) probes to enable live-cell membrane tension imaging. The molecular assemblies consist of a rigid tetrahedral DNA framework anchored with prescribed numbers of VIE probes. We find that VIE probes on the DNA framework retain their ratiometric fluorescence response characteristics in aqueous systems and on lipidic model membranes. Importantly, VIE assemblies exhibit distinct cell membrane targeting behaviors depending on the number of contact points between the molecular assemblies and the cell membrane. Especially, trivalent molecular assemblies can inhibit the internalization of the probes by the cells, a property absent in free VIE and mono/divalent molecular assemblies, thereby achieving targeted and prolonged retention on the cell membrane. Using the trivalent molecular assemblies, we successfully achieve ratiometric fluorescence imaging of cell membrane tension using confocal laser scanning microscopy, revealing the potential of such multifunctional mechanical-sensitive probes for live-cell applications.

Anthraldehyde-based aggregation induced emissive probe for hydroxylamine detection and latent fingerprint imaging

Anthraldehyde-based aggregation induced emissive probe for hydroxylamine detection and latent fingerprint imaging

Aggregation Induced Emission Based Luminogenic (AIEgenic) Probes for the Biomarker Detection.

Various biomarkers such as proteins play key roles in controlling crucial biochemical processes. The critical concentration of the biomarkers is important to maintain a healthy life. In fact, imbalance in concentration or irregular activity of these can lead to various diseases like Cancer, Alzheimer's etc. Therefore, the disease related biomarkers and their timely detection would be the key to control the illness. In the literature, a few fluorescent activity-based probes for the detection of such biomarkers are available.In this regard, several fluorescent probes are of great choice. Although these fluorescent probes face certain challenges such as aggregation caused quenching, which heavily affects the sensitivity and photostability is another major concern for many fluorescent probes. To overcome these challenges aggregation-induced emissive fluorescent probes could be an excellent alternative. Aggregation induced emissive luminogens (AIEgens) offer higher signal to noise ratios and found to possess better photostability during sensing and imaging. In the present review we have summarized the development of AIEgenic probes for sensing and imaging of disease related biomarkers.

Near-Infrared Visualization of NAD(P)H Dynamics in Live Cells and Drosophila melanogaster Larvae Using a Coumarin-Based Pyridinium Fluorescent Probe.

A near-infrared fluorescent probe, A, was designed by substituting the carbonyl group of the coumarin dye's lactone with a 4-cyano-1-methylpyridinium methylene group and then attaching an electron-withdrawing NADH-sensing methylquinolinium acceptor via a vinyl bond linkage to the coumarin dye at the 4-position. The probe exhibits primary absorption maxima at 603, 428, and 361 nm, and fluoresces weakly at 703 nm. The addition of NAD(P)H results in a significant blue shift in the fluorescence peak from 703 to 670 nm, accompanied by a substantial increase in fluorescence intensity. This spectral shift is attributed to the transformation from an A-π-A-π-D configuration to a D-π-A-π-D pyridinium platform in probe AH, owing to the addition of a hydride from NADH to the electron-accepting quinolinium acceptor producing the electron-contributing 1-methyl-1,4-dihydroquinoline donor in probe AH. This conclusion is supported by theoretical calculations. The probe was utilized to investigate NAD(P)H dynamics under various conditions. In HeLa cells, treatment with glucose or maltose resulted in a substantial elevation in near-infrared emission intensity, suggesting increased NAD(P)H levels. Chemotherapeutic agents including cisplatin and fludarabine at concentrations of 5, 10, and 20 μM brought about a dose-dependent increase in emission intensity, reflecting heightened NAD(P)H levels due to drug-induced stress and cellular damage. In vivo experiments with hatched, starved Drosophila melanogaster larvae were also conducted. The results showed a clear relationship between emission intensity and the levels of NADH, glucose, and oxaliplatin, confirming that the probe can detect variations in NAD(P)H levels in a living organism. Our investigation also demonstrates that NAD(P)H levels are significantly elevated in the cystic kidneys of ADPKD mouse models and human patients, indicating substantial metabolic alterations associated with the disease. This near-infrared emissive probe offers a highly sensitive and specific method for monitoring NAD(P)H levels across cellular, tissue and whole-organism systems. The ability to detect NAD(P)H variations in reaction to varying stimuli, including nutrient availability and chemotherapeutic stress, underscores its potential as a valuable resource for biomedical research and therapeutic monitoring.

Benzo[c,d]Indole-Quinoline-Based Deep-Red Emissive Probes for Live-Cell Imaging of Nucleolar RNA.

Small molecular weight fluorescent probes capable of binding to RNAs have been powerful tools for understanding the intracellular behaviors of RNAs. In this chapter, we describe the fluorescence imaging of nucleolar RNA in living cells using deep-red emissive probes with benzo[c,d]indole-quinoline (BIQ) monomethine cyanine scaffolds. These probes feature a significant fluorescence "off-on" ability upon binding to RNAs (>100-fold) in the deep-red spectral region (λem>650nm). In addition, they have many advantages for fluorescence sensing of RNAs and nucleolar RNA imaging, such as longer emission wavelength, higher photostability, and better counterstaining compatibility for live cell imaging, compared to a commercially available RNA-binding probe, SYTO RNA select.

Theoretical guiding with molecular docking for the screening of high-sensitive AIE probes for specific protein detection

The detection of proteins is crucial in the fields of disease diagnosis and drug development. Detection of proteins by aggregation-induced emission (AIE) probes is a quick and convenient method. However, the current AIE probes for the specific protein detection mainly depended on experimental test, lacking a guiding strategy. This study presented a novel approach to design AIE probes for detecting protein using molecular docking depending on AIE luminescence mechanism of the restriction of intramolecular motions. As an example to show its feasibility, three AIE probes with pyrrolo[3,2-b]pyrrole motif were designed and synthesized. The binding of the three probes to nine common proteins were predicted in advance using the molecular docking technique, obtaining information including intermolecular forces, binding energy, and binding sites between probes with proteins. The prediction results were verified through experimental data, and the mutual comparation of experimental and molecular docking results confirmed the reliability of the information provided by the molecular docking technique. Furthermore, the probes TPPP-2Na and TPPP-4Na exhibited limit of detection as low as 0.33 μg/mL for BSA and 0.35 μg/mL for HSA, respectively. The study revealed an innovative approach for the screening and molecular design of AIE probes for the detection of proteins.

Fluorescence Detection and Inhibition Mechanisms of DNTPH on Aβ42 Oligomers Characterized as Products in the Four Stages of Aggregation.

Aβ42 aggregation was implicated in the pathogenesis of Alzheimer's disease (AD) without effective treatment available currently. Future efforts in clinical trials should instead focus on applying those antiamyloid treatment strategies to the preclinical stage and "the earlier, the better". How to identify and inhibit Aβ42 oligomers in the different stages of aggregation is therefore becoming the key to controlling primary aggregation and consequent AD development. Aggregation-induced emission probe DNTPH was demonstrated recently, enabling detection of amyloid at wavelengths up to 710 nm and exhibiting strong inhibitory effects on Aβ fibrosis at low dose. However, the detection and inhibition mechanisms of Aβ oligomers at various early stages of aggregation remain unknown. To this end, we built four different morphologies of Aβ42 pentamers characterized by products in monomeric aggregate (PM), primary nucleation (PP), secondary nucleation (PS), and fibril stages (PF) to explore the distinguishable ability and inhibition mechanisms of DNTPH with different concentrations upon binding. The results showcased that DNTPH does detect the four different Aβ42 oligomers with conspicuous fluorescence (λPM = 657 nm, λPP = 639 nm, λPS = 630 nm, and λPF = 648 nm) but fails to distinguish them, indicating that additional improvements are required further for the probe to achieve it. The inhibition mechanisms of DNTPH on the four Aβ42 aggregation are however of amazing differences. For PM and PP, aggregation was inhibited by altering the secondary structural composition, i.e., by decreasing the β-sheet and toxic turn (residues 22-23) probabilities, respectively. For PS, inhibition was achieved by segregating and keeping the two disordered monomeric species (PSM) away from the ordered secondary seed species (PSF) and consequently blocking further growth of the PSF seed. The inhibition mechanism for PS is first probed and proposed so far, as far as we know, and the corresponding aggregation stage of PS is the most important one among the four stages. The inhibition of PF was triggered by distorting the fibril chains, disrupting the ordered fibril surface for the contact of monomers. In addition, the optimal inhibitory concentrations of DNTPH for PM, PP, and PF were determined to be 1:3, while for PS, it was 1:5. This outcome offers a novel perspective for designing drugs targeting Aβ42 oligomers at different aggregation stages.

Effect of input power on plasma expansion and ion acceleration in a radio-frequency plasma thruster

Exploring the physics of low pressure plasmas expanding in a diverging magnetic nozzle, and the resulting acceleration mechanisms, plays an important role in the development of a new-type of electrode-less plasma propulsion systems. This study discusses the effects of input power on plasma expansion and ion beam acceleration in a magnetic nozzle electrode-less plasma thruster. The experiments were conducted in a radio-frequency magnetic nozzle plasma device at The University of Auckland with four different power configurations PRF. Different plasma diagnostics were used to measure the characteristics of the plasma plume. A planar Langmuir probe was used to measure the floating potential and ion saturation current both in the plasma source and in the expansion chamber. The potential drop in the plasma source was obtained with an emissive probe. A retarding field energy analyser was employed to evaluate the local plasma and ion beam potentials, the ion energy distribution functions, and to estimate the ion beam speed in the expansion region. Measurements showed that, as expected, increasing the power input resulted in a higher plasma and supersonic ion density, while the ion beam speed did not increase further for PRF>100W. Interestingly, and contrary to the idealised physical model, the ion sonic transition did not occur at the magnetic nozzle throat, but instead close to the geometrical expansion point, i.e. near the interface between the source tube and the expansion chamber. This feature would result in a lower performance of the thruster given the reduced expansion ratio. An E-H mode change is also observed to occur in the device with increasing radio-frequency power that would help explain the different plasma characteristics observed at the 200W transition point.

Rapid and Efficient Dual Detection Of Zn2+ Ions and Oxytetracycline Hydrochloride Using a Responsive Fluorescent "On-Off" Sensor Based on Simple Salen-Type Schiff Base Ligand.

This research has progressed to an effective detection chemosensor of zinc, aluminum ions and oxytetracycline hydrochloride antibiotic based on the fluorescence technique. A straightforward method utilizing microwave irradiation was employed to synthesize the salen-type Schiff base ligand N,N'-bis(salicylaldehyde)4,5-dichloro-1,2-phenylenediamine (H2I), providing a good 70 % yield. In ethanol, the H2I sensor demonstrated remarkable rapidity, selectivity, and sensitivity in detecting zinc ions. The fluorescence spectrum exhibited a 44-fold substantial enhancement at 522 nm and achieved a low limit of detection (LOD) of 1.47 μM. Furthermore, the H2I probe's emission intensity increased by 124 times when compared to the ligand's ability to detect Al3+ ions at 494 nm with a LOD value of 7.4 μM. Additional research was done using the H2I probe's effective Zn2+ detection capability. The ability to recognize zinc ions in different real water samples demonstrated a recovery rate of 98.67 % to 103.31 %. Interestingly, a naked-eye visible fluorescence color of H2I solution impregnated filter papers turned colorless into yell ow under UV irradiation by adding Zn2+ ions, renders it suitable for developing a practical zinc ion detection kit test. In particular, the I-Zn2+ complex effectively quenched the fluorescence toward oxytetracycline hydrochloride (OTC) with a LOD value of 1.49×10-2 μM in DMSO:H2O (6 : 4, v/v). This is a novel and effective procedure for sensing OTC antibiotic by the I-Zn2+ complex. These findings hold immense potential for the development of dual fluorescent probes, thereby enhancing sensitivity and specificity in identifying metal ions and antibiotics in a wide range of applications.

Synergistically Activated Aggregation-Induced Emission Probe for Precise In Situ Staining of Lipids in Atherosclerotic Plaques.

Visualizing the localization and distribution of lipids within arteries is crucial for studying atherosclerosis. However, existing lipid-specific probes face challenges such as strong hydrophobicity and nonspecific staining of lipophilic organelles or tissues, making them impractical for the precise identification of atherosclerotic plaques. To address this issue, we design a synergistically activated probe, Cbz-Lys-Lys-TPEB, which responds to cathepsin B (CTB) and H2O2 for the in situ generation of aggregation-induced emission luminogens (AIEgens). This enables specific staining of lipids within arteries and precise imaging of atherosclerotic plaques. The probe combines a tetraphenylethene building block with a hydrophilic peptide sequence (Cbz-Lys-Lys) and phenylboric acid module, providing excellent water solubility and fluorescence quenching in a molecular dispersion state. Upon interaction with H2O2 and CTB within plaques, the hydrophilic Cbz-Lys-Lys-TPEB probe is specifically cleaved and converted into hydrophobic AIEgens, leading to rapid aggregation and significant fluorescence enhancement. Interestingly, the in situ-liberated AIEgens display distinct lipid binding ability, effectively tracking the location and distribution of lipids in plaques. This synergistic target-activated AIEgen liberation strategy demonstrates significant feasibility for the reliable and accurate identification of atherosclerotic plaques, holding tremendous potential for clinical diagnosis and risk stratification of atherosclerosis.

Cyanide and chloroform detection through J-aggregates based aggregation induced emission probe with real sample applications

Isopthalamide based probe DPI has been synthesized by an easy two-step substitution reaction. Unique fluorescence properties of probe DPI were exploited for sensing of CNˉ and chloroform. Various spectroscopic techniques such as NMR, LC-MS, SEM, DLS, UV-Vis. and fluorescence spectroscopy in combination with DFT studies were used to confirm efficient detection of CN‾ through a non-covalent interaction of cyanide with probe. Furthermore, probe showed fluorescence emission at 360 nm which shifted significantly to 415 nm upon addition of water exhibiting unique AIE characteristics and formation of desired J-aggregates. Mechanistically, CN‾ and chloroform were selectively detected through fluorescence quenching with 9 nM and 0.2 % v/v limit of detection (LOD), respectively. Photoinduced electron transfer (PET) was proven to be involved as a sensing mechanism. Moreover, DPI exhibited interesting solvatochromism properties. DPI was proven to be a highly sensitive probe which showed solid-state and vapor phase on-field detection of CN‾. Similar sensing behavior of DPI probe towards CN‾ was seen in food and water samples.

Elucidating Polyphosphate Anion Binding to Lanthanide Complexes Using EXAFS and Pulsed EPR Spectroscopy.

Reversible anion binding to lanthanide complexes in aqueous solution has emerged as an effective method for anion sensing. Through careful design of the organic ligand, luminescent lanthanide complexes capable of binding biologically relevant anions in a bidentate or monodentate manner can be realized. While single-crystal X-ray diffraction analyses and NMR spectroscopy have revealed the structural geometry of several host-guest complexes, the challenge remains in designing preorganized lanthanide receptors with enhanced anion selectivity for broader applications in diagnostics and bioimaging. To address this challenge, innovative and complementary methods to investigate host-anion binding geometry are becoming increasingly important. Herein, we demonstrate the combined use of Eu L3-edge extended X-ray absorption fine structure (EXAFS) and electron paramagnetic resonance (EPR) spectroscopy to elucidate the binding of nucleoside phosphates (ATP, ADP, and AMP) to a cationic lanthanide complex. We establish that ATP unequivocally binds the lanthanide center in a bidentate manner in water, while ADP adopts both bidentate and monodentate modes, and AMP binds in a monodentate manner. This interdisciplinary approach provides deeper insight into lanthanide host-guest chemistry in solution, laying the groundwork for designing emissive probes that undergo specific anion-induced structural changes and elicit desired optical responses upon binding.

Monitoring of mercury ion in environmental media and biological systems using a red emissive fluorescent probe with a large Stokes shift

The development of practical fluorescent probe for detecting toxic mercury ions (Hg2+) is desirable for environmental assurance and public health. In this study, a new red emissive fluorescent probe (KJL) was designed and synthesized for monitoring trace Hg2+ both in vitro and in vivo with distinct features including ideal response rate (within 4 min), red emission (596 nm), large Stokes shift (162 nm), highly sensitivity (LOD = 4.79 nM) and excellent specificity. KJL also validated the good capability for accurately monitoring trace Hg2+ levels in actual samples (faucet water, drinking water, river water, lake water, urine and serum) and possessed the eye-catching ability in visualization of Hg2+ under environmental/biological conditions, which revealed the great potential of this red-emitting fluorescent probe for practical applications in complex environmental and biological systems.

Ratiometric fluorescence probe based on boric acid-modified carbon dots and alizarin red for sensitive and rapid detection of glyphosate.

Bycombining boric acid-modified carbon dots (p-CDs) and alizarin red (ARS), a double emission probe p-CDs@ARS with fluorescence at 410nm and 600nm is designed for the detection of glyphosate. When Cu2+ is added, it binds with ARS to cause ARSreleasefrom p-CDs@ARS, which decreases the fluorescence at 600nm. However, in the presence of glyphosate, glyphosate competes to thebinding ofCu2+, releasing ARS to bind with p-CDs again. Therefore, the fluorescence of 600nm recovers. Based on this, the fluorescence of 410nm and 600nm act as the reference and response signal, respectively,achieving the ratiometric fluorescence detection of glyphosate. The linear range of glyphosate detection is 0.5-50µM with a limit of detection at 0.37µM which is well below the maximum residue limit for glyphosate in food. When the probe is used to detect the glyphosate residue in Pearl River water and cucumber, the detection results are well consistent with those detected by HPLC. The established method based on p-CDs@ARS has the advantages that the assembly of ratiometric fluorescence probe is simple, and the detection speed is fast. Additionally, a typical INHIBIT logical system has been successfully constructed based on glyphosate, Cu2+, and the fluorescence signal of p-CDs@ARS.

Aggregation-Induced Emission (AIE) Probes in Fluorescent Sensing: Progress and Applications for Pesticide Detection

Pesticide residues pose significant risks to human health and the environment, emphasizing the need for sensitive detection and analysis methods. Fluorescence-based sensors, particularly those utilizing aggregation-induced emission (AIE) fluorophores (AIEgens), have demonstrated exceptional performance in this area. This review summarizes key advancements in pesticide detection sensors based on AIEgens, detailing their luminescence mechanisms and fluorescence sensing principles. It explores various applications of AIEgens in fluorescence sensors, including organic small-molecule sensors, nanocomposite sensors, metal-organic framework sensors, supramolecular sensors, fluorescent porous organic polymer sensors, and lateral flow immunoassay sensors, with specific examples illustrating their detection mechanisms and performance. This review also discusses current challenges and future perspectives for the development of these sensors. We anticipate that this review will serve as a valuable and timely resource for researchers working to advance the development and application of AIEgens-based sensors in pesticide detection.

Plasma potential and ion energy characteristics in BP-HiPIMS discharge with double layer

Abstract As an emerging ion acceleration plasma source, the bipolar-pulse high power impulse magnetron sputtering (BP-HiPIMS) discharge has been widely studied by academia and industry due to its ability to adjust the ion kinetic energy. Formation of the double layer (DL) potential structure during the BP-HiPIMS positive pulse is vital for accelerating ions, but its structural characteristics are still unclear. In this work, to understand the DL characteristics affected by various discharge parameters, the evolution of plasma potential V p and ion energy in BP-HiPIMS discharge with copper target has been investigated systematically using an emissive probe and mass spectrometer together. Spatial plasma potential measurements show that the DL is established in front of the target during the positive pulse, whose boundary potential drop U DL to accelerate ions can be increased to ∼60 V at a lower operating gas pressure (p= 0.6 Pa) and a higher applied positive pulse voltage (U + = 200 V). The ignition onset time of DL after applying the positive pulse can be shortened to ∼25 μs by decreasing the gas pressure and increasing the positive pulse voltage or negative pulse duration. After DL ignition, a group of high-energy copper ions with energy higher than the surrounding plasma potential can be recognized in the ion energy distribution function curves in the downstream plasma. This result illustrates that the copper ions can be ionized in the high-potential plasma region and be accelerated by the DL boundary potential drop. In addition, a global current balance model of the DL in BP-HiPIMS is developed, which suggests that the U DL can be well adjusted by increasing the positive pulse voltage U + especially for U + > 200 V as verified by the experimental potential measurements. All results suggest that the copper particles play an important role in the formation of DL and the DL plays an important role in accelerating copper ions.

Monitoring a batch crystallization process by acoustic emission

Crystallization is regarded as an important unit operation for separation and purification, as well as the production of solid particles with specified end-use properties. However, it is still difficult to control or to optimize the crystallization process due to the complexity of the coupled phenomena taking place simultaneously in the liquid and solid phases. In order to overcome such drawback, different analytical technologies have been implemented in the literature for monitoring (in situ, at best) the key crystallization parameters, e.g. solute concentration and crystal size distribution, which in turn enhances the comprehension about this operation. In our work, a multi-probe monitoring system composed of acoustic emission, ATR-FTIR and imaging probes was applied to the crystallization of a model system (an aqueous solution of adipic acid) in a semi-batch crystallizer. The crystallization was carried out under two different feed rates and under vacuum or atmospheric pressure. The goal was to demonstrate the usefulness of the multi-probe system for monitoring the crystallization process and to study the influence of the process (stirring rotation speed, cooling speed, etc.) on the acoustic emission. As a matter of fact, the multi-probe system tracked information about the dissolved adipic acid in the liquid phase with the ATR-FTIR spectrometry, while the crystal size, shape and number were monitored with the help of the imaging probe (with image treatment methods). The acoustic emission probes were used to detect the beginning of crystallization and the phenomena of crystal growth and agglomeration