We experimentally demonstrate the generation and transmission of probabilistic shaping (PS) 64-ary quadrature amplitude modulation (64-QAM) discrete multi-tone (DMT) signal with low-density parity-check (LDPC)-coded modulation based on two-stage bit-weighted distribution matching (TS-BWDM) in a high-speed unamplified intensity modulation and direct detection system. The TS-BWDM scheme is based on a two-stage bit-weighted inversion algorithm, which has the advantages of no complex multiplication and division operations and low hardware implementation complexity. As the key content of TS-BWDM, before FEC coding, the bit-weighted intervention operation is used to change the bit probability of a specific sequence in the parallel binary sequence. The two-stage bit-weighted inversion operation and one-stage weight-labeling bit insertion is performed for achieving the target symbol probability distribution. As a result, it does not add a significant amount of extra redundant bits. Additionally, the high-accuracy training symbols-based sampling frequency offset (SFO) estimation method and digital interpolation are used to compensate SFO. In our experiments, the TS-BWDM-based PS-64QAM DMT signals with a net rate of 157.3-Gb/s transmission over 10-km non-zero dispersion- shifted fiber can be achieved. The system performance is investigated under two LDPC code rates (3/4 and 9/10) and three PS parameter values (k = 3, 5 and 9). The experimental results show that the receiver power sensitivity and the system fiber nonlinear effect tolerance can be significantly improved compared with uniformly-distributed signals.