Background An increasing number of occupational injuries occur due to work-related musculoskeletal disorders (WRMSDs) in both developing and industrialized countries. These disorders have an impact on workers' health as well as society at large, posing medical, occupational, and economic challenges. Objectives This research assessed work-related musculoskeletal disorders and its risk factors among Bus drivers for private long-distance Transport Companies in Port Harcourt, Nigeria. Materials and Methods This study employed a cross-sectional study design. The multi-stage sampling technique was used to sample 338 long-distance bus drivers in the city of Port Harcourt. Participants' demographic data and information regarding work related musculoskeletal disorders were collected using a modified version of the Standardized Nordic Musculoskeletal Survey questionnaire. Descriptive statistics determined prevalence and inferential statistics was used to determine associated risk factors of WRMSD. The p-value of the test of statistical significance was set at ≤0.05. The participants were all male. Result 58.6% of the research sample had WRMSDs, according to the findings. After logistic regression analysis, only marital status (AOR=5.714, 95% CI for AOR=3.046- 10.721; p=0.001), prolonged sitting of ≥8 hours, (AOR=0.350; 95% CI for AOR=0.166-0.741; p=0.006) and previous history of pain in a previous occupation (AOR=5.714, 95% CI for AOR=3.046-10.721; p=0.001), were significantly associated with the prevalence of WRMSD. Conclusion WRMSDs are prevalent in private long-distance bus drivers and are strongly correlated with marrital status, prolonged sitting times (more than eight hours), and a history of muscle or joint discomfort in a prior job.