Event Abstract Back to Event Early local IL-12p40/p(40)2 production and intensity of peritoneal inflammation correlate with susceptibility to pristane induced arthritis Cristiano Rossato1, Layra L. Albuquerque1, Orlando G. Ribeiro1, Nancy Starobinas1, Mônica S. Ferreira1, Wafa Cabrera1, Andrea Borrego1, Olga M. Ibañez1, Marcelo De Franco1 and José R. Jensen1* 1 Instituto Butantan, Brazil Genetically selected HIII (high antibody producer) mice are resistant, while LIII (low antibody producer) are susceptible (1) to pristane-induced arthritis (PIA). The peritoneum (PerC), site of PIA induction, is rich in myeloid and lymphoid cells and might be the site where the genetic divergence in susceptibility of HIII/LIII mice would translate into functional differences, affecting the early inflammatory cell response and cytokine balance. Mice were injected with 0.5mL pristane i.p. and after 48h, 4, 7, 15 or 30 days, the PerC was washed with RPMI1640+10%FBS. The supernatant was used for cytokine ELISA and cells were counted in hemocytometric chambers and phenotyped by FACS. Inflammatory cell infiltration was highest in LIII mice at 7 days, with increased numbers of most cell populations. Leukocyte infiltration in HIII mice, consisting mostly of neutrophils, increased slowly until d30, while monocytes/macrophages and lymphocytes were reduced. Dendritic cells and T cells increased only in pristane treated LIII mice. Both lines produced similar amounts of CCL-2, IL-1beta, IL-6, IL-4, and low levels of IL-12p70 after pristane injection, while IFN-gama was not detected. However, IL-12p40/p(40)2 was produced only in LIII mice, increasing from d4 to d30. Our results suggest that the divergent susceptibility of HIII/LIII mice might be linked to their distinct peritoneal response to pristane, and that IL-12p40/p(40)2, which is mainly produced by dendritic cells, might be involved by promoting dendritic cell recruitment and migration to lymph nodes, thereby increasing the potential of autoreactive T cell activation in susceptible LIII mice. Acknowledgements Brazilian National Research Council (CNPq, fellowship n.º137108/2009-5) and São Paulo Research Foundation (FAPESP). References Jensen JR, Peters LC, Borrego A, Ribeiro OG, Cabrera WH, Starobinas N, Siqueira M, Ibañez OC, and De Franco M. Involvement of antibody production quantitative trait loci in the susceptibility to pristane-induced arthritis in the mouse. Genes Immun. (2006) 7:44-50. Keywords: pristane, experimental arthritis, genetically selected mice, peritoneal inflammation, cytokines. Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Rossato C, Albuquerque LL, Ribeiro OG, Starobinas N, Ferreira MS, Cabrera W, Borrego A, Ibañez OM, De Franco M and Jensen JR (2013). Early local IL-12p40/p(40)2 production and intensity of peritoneal inflammation correlate with susceptibility to pristane induced arthritis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00984 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. José R Jensen, Instituto Butantan, São Paulo, Brazil, jose.jensen@butantan.gov.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Cristiano Rossato Layra L Albuquerque Orlando G Ribeiro Nancy Starobinas Mônica S Ferreira Wafa Cabrera Andrea Borrego Olga M Ibañez Marcelo De Franco José R Jensen Google Cristiano Rossato Layra L Albuquerque Orlando G Ribeiro Nancy Starobinas Mônica S Ferreira Wafa Cabrera Andrea Borrego Olga M Ibañez Marcelo De Franco José R Jensen Google Scholar Cristiano Rossato Layra L Albuquerque Orlando G Ribeiro Nancy Starobinas Mônica S Ferreira Wafa Cabrera Andrea Borrego Olga M Ibañez Marcelo De Franco José R Jensen PubMed Cristiano Rossato Layra L Albuquerque Orlando G Ribeiro Nancy Starobinas Mônica S Ferreira Wafa Cabrera Andrea Borrego Olga M Ibañez Marcelo De Franco José R Jensen Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.